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Citations for Agilent :
1 - 50 of 196 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... GPx7 and GPx8 Cys-Ala mutants were constructed by using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Microbiology 2022Quote: ... A plasmid encoding inactive RHDV2 3CLpro was generated by substituting the nucleophilic Cys with Ala in the active site of 3CLpro in pcDNA3-RHDV2 using site-directed mutagenesis (Agilent’s Quik Change mutagenesis kit ...
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bioRxiv - Biochemistry 2021Quote: ... Cys to Ala mutants of Flag-tagged DJ-1 were using QuikChange Ⅱ Site-Directed Mutagenesis kit (Agilent Technologies, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Mutations in Hs ProXp-ala and Cc ProXp-Ala were introduced using either QuikChange Site-Directed Mutagenesis (Agilent) or Site-directed ...
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bioRxiv - Cancer Biology 2022Quote: ... The RIOK1 Ser22 to Ala mutant and Ser21/22 to Ala double mutant were generated using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Catalog #210518). Primers were designed using the QuikChange Primer Design website ...
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bioRxiv - Cell Biology 2021Quote: SopB and the inactive version were cloned into pESC-LEU (Agilent Technologies) for galactose inducible expression.
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... 2,4-Dinitrophenol (DNP; SantaCruz, Dallas, Texas, US) or Oligomycin (Agilent, Santa Clara, California, US) were applied at indicated concentrations for 24 hours to inhibit mitochondrial respiration ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 HO-1 +/+ and HEK293 HO-1 -/- were seeded at a density of 2.5 × 104 cells/well on a poly-D-lysine (#P6407-5MG) coated Seahorse XF96/XF Pro cell culture microplate (#204624-100, Agilent) on the day prior to the analysis ...
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bioRxiv - Immunology 2020Quote: ... The CyldS7A ser-to-ala mutations were generated using Quikchange Multi Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Molecular Biology 2020Quote: Phosphorylated residues of Spt5 were substituted with Ala or Asp using site-directed mutagenesis kits (Agilent Technologies), the oligonucleotides listed in Supplementary Table 1 ...
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bioRxiv - Immunology 2021Quote: ... Cy-dye labelling and hybridization to Agilent High Definition 4×44k array (Agilent technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... CDB0573K: http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) was constructed from a 129SVJ genomic clone (STRATAGENE, CA). Exon 1 of the Cnot9 locus was targeted and replaced by LacZ and neomycin resistance gene by electroporating TT2 ES cells (Yagi et al. ...
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bioRxiv - Cell Biology 2024Quote: ... The substitution of Asp37 to Ala in pCMV-FLAGWRNIP1 was introduced using the Quick-change XL kit (Stratagene) with mutagenic primer pairs designed according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: dCas9-Cys protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with plasmid 10xHis-MBP-TEV-S ...
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bioRxiv - Microbiology 2020Quote: ... Mx3000/Mx Pro software (Stratagene, USA) was used to construct a melting curve ...
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bioRxiv - Immunology 2024Quote: ... The xFe96 Pro sensor cartridge (Agilent) was hydrated overnight with sterile water at 37°C in a non-CO2 incubator ...
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bioRxiv - Bioengineering 2024Quote: ... Cholesteryl esters and triglycerides were eluted on an aminopropyl column (Bond Elut LRC-NH2, 500mg) (Agilent Technologies). Fatty acids from each fraction were analyzed as their methyl esters after a direct transesterification with acetyl chloride/methanol on a 7890B gas chromatograph coupled to a 5977A Mass Selective Detector (Agilent Technologies ...
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bioRxiv - Immunology 2023Quote: ... 2µg amplified cDNA was Cy-labeled using the SureTag DNA labeling kit (Agilent, Waldbronn, Germany). The Cy5-labeled cDNA was hybridized overnight to 8 x 60K 60mer oligonucleotide spotted microarray slides (Agilent Technologies ...
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bioRxiv - Biochemistry 2021Quote: Tpm isoforms Tpm1.6 and Tpm3.1 (with and without Met-Ala-Ser at the N-terminus) were expressed in ArcticExpress(DE3) RIL cells (Agilent Technologies), grown in Terrific Broth (TB ...
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bioRxiv - Physiology 2021Quote: ... Amino acid exchanges from Thr to Ala at position 972 in murine TRPC5 were introduced by site-directed mutagenesis using the QuikChange system (Stratagene). The cDNA constructs used in the present work were confirmed by Sangar sequencing (Macrogen ...
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bioRxiv - Cell Biology 2024Quote: ... XFe96/XF Pro Cell Culture microplate (Agilent) was coated with 0.1mg/ml Poly-D-Lysine (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... and Leu 305 in PP6C were deleted by inserting a stop codon using Quick Change Lightning Kit (Agilent) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... enterica WbaP Cys-pair variants were generated using primers designed in the quikchange primer tool (Agilent). Successful mutations were confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis for Cys238>Ala active site substitution in scpA was performed using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) using oligonucleotides MP_scpA1 and MP_scpA2 [49].
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bioRxiv - Biochemistry 2022Quote: Offline N-glycan analysis was done using AdvanceBio Gly-X N-glycan prep with InstantPC (GX96-IPC, Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Offline N-glycan analysis was done using AdvanceBio Gly-X N-glycan prep with InstantPC (GX96-IPC, Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... analysis of intact N-glycans was performed using 2-aminobenzamide (2-AB) labeling and separation on a Zorbax NH2 column (Agilent), essentially as described elsewhere [11 ...
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bioRxiv - Immunology 2023Quote: ... analysis of intact N-glycans was performed using 2-aminobenzamide (2-AB) labeling and separation on a Zorbax NH2 column (Agilent), essentially as described elsewhere (Bigge ...
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bioRxiv - Immunology 2021Quote: ... in order to modify its DAG discrimination capacity 17 by replacing Gly652 with Ala (G652A) using the Quick-Change XL site-directed mutagenesis kit (Stratagene, La Jolla, CA) and the oligonucleotides described in Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... a Seahorse XFe96/XF Pro Cell Culture microplate (Agilent) was coated with 0.1mg/ml Poly-D-Lysine (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Data analyses were performed with RTCA Software Pro (Agilent).
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bioRxiv - Microbiology 2024Quote: ... Mutation of the catalytic active site at position 138 (Ser-138 to Ala-138) of RLip was introduced via the QuikChange II XL site-directed mutagenesis kit (Agilent technologies, Cat-No.: 200521-5) according to the manufacturer’s instructions using the using Forward primer 5’-CTTTTAACACCAGCGGCCACGCGCTGGGCGCGATTATC-3’ and Reverse primer 5’-GATAATCGCGCCCAGCGCGTGGCCGCTGGTGTTAAAAG-3’ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 0.5 μL of AccuScript Hi-Fi (Agilent Technologies) was added up to a 10 μL volume ...
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bioRxiv - Systems Biology 2023Quote: ... A 300 × 7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
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bioRxiv - Microbiology 2022Quote: ... A 300×7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
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bioRxiv - Genomics 2023Quote: ... were used to assess the Hi-C libraries (Agilent). The libraries were sequenced at New York Genome Center (NYGC ...
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bioRxiv - Evolutionary Biology 2020Quote: A mutant version of hA5 with a single N-terminal Cys residues were generated via sitedirected mutagenesis using the QuikChange lightning system (Agilent). The Cys was introduced in the Ser-Asn tag leftover from TEV protease cleavage as Ser-Asn-Cys ...
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bioRxiv - Biophysics 2023Quote: ... derivative encoding two cysteine residues at positions 366 and 511 [constructed from plasmid pGEMD (-Cys)46 by use of site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit; Agilent) to replace codons 366 and 511 by a codon encoding cysteine residue] ...
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bioRxiv - Biochemistry 2024Quote: ... were introduced into all TE expression plasmids in place of codons for the catalytic Ser or Cys residues using the QuikChange XL Site-Directed Mutagenesis kit (Agilent), optimized for GC-rich sequences (JuvEV TE S1637amb ...
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bioRxiv - Cancer Biology 2023Quote: ... Percent cytotoxicity was calculated using RTCA Software Pro immunotherapy module (Agilent).33
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bioRxiv - Molecular Biology 2024Quote: ... along with Seahorse XFe96/XF Pro FluxPak (Agilent, Cat# 103792-100), and Seahorse XF DMEM medium (without Phenol Red/pH 7.4/with HEPES/500 mL ...
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bioRxiv - Cancer Biology 2024Quote: ... Seahorse XFe96/XF Pro FluxPak Mini culture plates (Agilent, 103793-100) were coated with 50 μg/mL Poly-Lysine (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... along with Seahorse XFe96/XF Pro FluxPak (Agilent, Cat# 103792-100) and Seahorse XF DMEM medium (without Phenol Red/pH 7.4/with HEPES/500 mL ...
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bioRxiv - Developmental Biology 2024Quote: Mitochondrial respirometry was performed using the Seahorse XF Pro Analyzer (Agilent). Cells were grown on tissue culture plates and 24 hours before testing ...
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bioRxiv - Neuroscience 2024Quote: ... The OCR data were analyzed with XFe Wave Pro software (Agilent), normalized to total cell protein levels (based on the BCA assay) ...
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bioRxiv - Biophysics 2020Quote: ... The desired base pairs coding for Cys were introduced using overlapping primers with the QuikChange II site-directed mutagenesis kit (Agilent Technologies) and were confirmed by DNA sequencing (GENEWIZ ...
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bioRxiv - Biophysics 2023Quote: ... Respirometry on HEK293 cells were performed on a SeaHorse XF Pro (Agilent) using the real-time ATP rate assay kit (103591-100 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and raw hybridization signals were extracted (GenePix Pro 6.0 software, Agilent Technologies). Transcription-level data were processed and analyzed using the LIMMA 2.16 software package (https://www.bioconductor.org/packages/3.3/bioc/html/limma.html) ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the Seahorse XFe96/XF Pro Sensor Cartridge plate (Agilent) and incubated overnight in a non-CO2 incubator at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and a 300×7.8 mm Hi-Plex Exchange column (Agilent, USA) was used to quantify glucose ...