Labshake search
Citations for Agilent :
451 - 500 of 3019 citations for Dengue Virus Serotype 1 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: Skd3 variants were expressed as an N-terminally MBP-tagged protein in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in 40mM HEPES-KOH pH=7.4 ...
-
bioRxiv - Cancer Biology 2020Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
-
bioRxiv - Synthetic Biology 2021Quote: Scanning mutant libraries were constructed using saturation mutagenesis using the QuikChange HT Protein Engineering System from Agilent following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... cells or tissue sections were incubated with Protein Block Serum-Free Ready-To-Use (Dako, #X 0909) for 30 min at room temperature ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein in the eluate was detected with a UV detector at 280 nm (Agilent Technology 1260 UV), a quasi-elastic light scattering (QELS ...
-
bioRxiv - Cell Biology 2021Quote: Constructs encoding the 6His-SUMO tagged proteins were transformed into chemically competent BL21(DE3) Escherichia coli (Agilent). A fresh bacteria colony was inoculated to 10 ml LB broth containing 200 μg/ml Ampicillin and 50 μg/ml Chloramphenicol and grown at 37°C overnight ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and slides were incubated with Dako Serum Free Protein Block (#X090930-2, Agilent, Santa Clara, CA, USA) at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins were diluted and scanned in a quartz cuvette with a Cary 60 UV-Vis spectrophotometer (Agilent). Extinction coefficients for Cas9 ...
-
bioRxiv - Biochemistry 2020Quote: ... All E protein variants were obtained by site-directed mutagenesis using QuikChange kit (Stratagene, La Jolla, California) and were confirmed by sequencing the plasmid DNA at Macrogen Company (Seoul ...
-
bioRxiv - Cell Biology 2021Quote: ... A53T and E46K α-synuclein proteins were expressed in BL2-Gold(DE3) competent cells (Agilent, Cat #230132). Protein expression was induced at log phase with 200 μM IPTG for 16 hours at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The immunoprecipitated proteins were analyzed using a precision HPLC unit (1100 series, Agilent, Santa Clara, CA, USA) equipped with a reverse-phase column and a micro-analytical UV detector system (SG Highteco ...
-
bioRxiv - Microbiology 2021Quote: ... 50 μl of protein (10 mg/ml) were injected into a size-exclusion column (Agilent BioSec-3) equilibrated in 50 mM Tris ...
-
bioRxiv - Molecular Biology 2021Quote: Protein expression was conducted in E.coli BL21 (DE3) Codon Plus cells (Agilent Technologies, San Diego, CA, USA). Induced cultures were grown at 18° C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... 600 ng of total protein was injected onto a home-packed PLRP column (PLRP-S) (Agilent Technologies), 10-µm particle size ...
-
bioRxiv - Biochemistry 2023Quote: The EQ2 mutations were introduced sequentially into each protein using QuikChange II Site-Directed Mutagenesis (Agilent Technologies) with all growth media supplemented with 0.4% (w/v ...
-
bioRxiv - Cancer Biology 2023Quote: ... The sections were rinsed in phosphate buffered saline (PBS) and blocked in Protein-Block buffer (DakoCytomation #0909) for 15 min ...
-
bioRxiv - Biochemistry 2023Quote: ... The maltose-binding protein (MAL) gene from Escherichia coli (gene identifier 7129408) in a pBlueScript KS (Stratagene) was amplified by PCR using T7 minimal promoter primers under the following conditions ...
-
bioRxiv - Cell Biology 2023Quote: ... metabolic labelling was performed in BOEC protein extracts using ENG mAb SN6h (Dako Denmark A/S, Denmark). Modifying methods of Pece et al,18 a 1 hour “pulse” with 50μCi/ml of 35S methionine ...
-
bioRxiv - Cell Biology 2023Quote: ... The results were subsequently introduced into the database for protein identification searches using Spectrum Mill (Agilent Technologies). All searches were carried out with “Drosophila melanogaster” as taxonomy in NCBInr database and 0.5 Da of tolerance on MS/MS fragments ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (NeuN, 1:20,000, Millipore, MAB377B; CD68, 1:1,000, Serotec, MCA1957S; GFAP, 1:10,000, Dako Z0334) were diluted in 0.3% Triton X in PBS and incubated for 12 hours at RT ...
-
bioRxiv - Cancer Biology 2020Quote: ... CD21 (DAKO, 1:25, CD23 (Leica, CD23-1B12, 1:50), CD4 (DAKO ...
-
bioRxiv - Cell Biology 2019Quote: ... progesterone receptor (CST 8757, 1:25; Dako A0098, 1:20), estrogen receptor (Santa Cruz sc-542 ...
-
bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Ki67 (1:50, clone MIB-1, Dako, M7240), mouse anti-INSR (1:50 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Anti-Ki-67 mouse (DAKO, clone MIB-1, 1:50); Anti-Ki-67 rabbit (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... GFAP (Biolegend, 829401, 1:1500 and Dako, Z0334, 1:1500), Nestin (Aveslabs ...
-
bioRxiv - Cancer Biology 2023Quote: ... thyroid transcription factor (TTF-1) (mouse, M3575, 1:100; Dako), and p40 (rabbit ...
-
bioRxiv - Immunology 2023Quote: ... stained for H&E and HSV-1 (1:1000, Dako), and analyzed by a veterinary pathologist (Dr ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1:1000, ab183741, abcam; n-Myc: 1:500, 51705 Cell Signaling Technology; CD45: 1:1000, 70257 Cell Signaling Technology; CD3: 1:2000 A0452 Dako/Agilent) diluted in TBST+5% goat serum in a humid chamber overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with the required primary antibody (ABri: 338 1:1000 from Ghiso lab; ADan: 5282 1:1000 from Ghiso lab; CD68: 1:150 DAKO; CR3/43: 1:100 DAKO) for 1 hour at room temperature ...
-
bioRxiv - Synthetic Biology 2021Quote: The LbCas12a library targeting the protein coding genes in PO1f were ordered as an oligonucleotide pool from Agilent Technologies Inc ...
-
bioRxiv - Cancer Biology 2021Quote: ... The assay was normalized with protein and analyzed with the XFe 2.0.0 software (Agilent Technologies Inc. CA, USA).
-
bioRxiv - Immunology 2021Quote: Biotinylated proteins were isolated from the organ homogenates using a Bravo AssayMap platform and AssayMap streptavidin cartridges (Agilent). The cartridges were equilibrated with ammonium bicarbonate (50mM ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary anti-CD31 antibody in Tris-HCl buffer containing stabilizing protein and 0.015 mol/L sodium azide (Dako Antibody Diluent ...
-
bioRxiv - Neuroscience 2020Quote: ... proteins were desalted online by reversed-phase chromatography on a Poroshell 300SB C3 column (1.0×75mm, 5um, Agilent Technologies ...
-
bioRxiv - Bioengineering 2021Quote: ... Sections were then incubated for 40 mins in 3% H2O2 TBST solution and blocked with protein block (Dako) for 20 mins ...
-
bioRxiv - Neuroscience 2020Quote: Primary antibodies used for immunofluorescence on brain sections were polyclonal rabbit anti-glial fibrillary acidic protein (GFAP; DAKO, Santa Clara ...
-
bioRxiv - Biochemistry 2020Quote: Affinity depletion of abundant serum proteins was carried out on a HP1290 HPLC system (Agilent, Santa Clara, CA) using the Multiple Affinity Removal Column Human 14 (MARS 14 ...
-
bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X 5 mins with 1X PBS and blocked in DAKO protein-free serum block (DAKO) overnight in a humidified chamber at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... the proteins were purified via reversed phase HPLC using a ZORBAX Eclipse Plus C18 (3.5 μm) column (Agilent) equilibrated with 0.1% trifluoroacetic acid (TFA) ...
-
bioRxiv - Cancer Biology 2021Quote: ... rinsed with water and then blocked in a serum-free protein block buffer for 15 min (Dako, X0909). Primary Axl antibody (RnD Systems ...
-
bioRxiv - Cancer Biology 2021Quote: ... rinsed with water and then blocked in a serum-free protein block buffer for 8 min (Dako, X0909). Primary CD133 antibody (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2020Quote: ... All antibody conjugates were run on a Bioanalyzer Protein 230 electrophoresis chip (Agilent Technologies, cat. no. 5067-1517) to verify successful conjugation.
-
bioRxiv - Microbiology 2020Quote: ... The peptides from proteins digested were desalted and concentrated with C18 reverse phase chromatography (OMIX C18, Agilent technologies) and peptides were eluted with 80% acetonitrile (ACN ...
-
bioRxiv - Biochemistry 2020Quote: ... 50 μl of a protein sample at 8.5-10 mg/ml was injected to a BioSEC3-300 (Agilent) or Superdex 75 10/300 GL increase column (GE Healthcare ...
-
bioRxiv - Immunology 2020Quote: ... To generate mutant proteins these vectors were altered by using QuickChange® XL site-directed mutagenesis (Agilent Technologies) according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were expressed in and purified from BL21-CodonPlus(DE3)pLysS Escherichia coli (Agilent Technologies, Santa Clara, CA) as described 48 and dialyzed against PLS buffer ...
-
bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 14,000 rpm on a tabletop centrifuge to remove aggregated proteins and then sequentially injected into a HPLC (Agilent) equipped with a C18 analytical column (Agilent Poroshell 120).
-
bioRxiv - Biophysics 2022Quote: ... The UV–visible spectra of purified NQO1 proteins were measured in a Cary spectrophotometer (Agilent Technologies, Waldbronn, Germany) and used to quantify NQO1 concentration and the content of FAD as described in (12) ...