Labshake search
Citations for Agilent :
151 - 200 of 3061 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... all PCRs were performed using PfuUltra II Fusion HotStart DNA polymerase (#600672, Agilent Technologies) and restriction enzymes were from New England Biolabs ...
-
bioRxiv - Genomics 2019Quote: ... and 0.25 μl (20 U/μl) of Herculase II fusion DNA polymerase (Agilent Technologies). The amplification profile consisted of an initial denaturation step (3 min at 95 °C ...
-
bioRxiv - Microbiology 2021Quote: ... tig was amplified from purified OG1RF genomic DNA using Pfu Ultra II polymerase (Agilent), digested with BamHI-HF/NheI-HF ...
-
bioRxiv - Microbiology 2020Quote: ... The high-fidelity Herculase II Fusion DNA Polymerase (Agilent, Santa Clara, CA, United States) was used for PCR amplification ...
-
bioRxiv - Microbiology 2022Quote: ... alleles were amplified from purified OG1RF genomic DNA using Pfu Ultra II polymerase (Agilent), digested with BamHI-HF and NheI-HF ...
-
bioRxiv - Biochemistry 2023Quote: ... All mutagenesis PCR reactions were amplified using PfuUltra II Fusion HS DNA Polymerase (Agilent). The parental (template ...
-
bioRxiv - Cell Biology 2020Quote: ... ∼100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2021Quote: PCR amplification of inserted sgRNAs from the genomic DNA was done with the Herculase II Fusion DNA Polymerase (Agilent Technologies) using the primers oMCB-1562 and oMCB-1563 ...
-
bioRxiv - Microbiology 2020Quote: ... The first reaction resulted in a plasmid containing 2,000 bp-long homologous arms (amplified from the genomic DNA of V. dahliae isolate Ls.17 using the Herculase II Fusion DNA polymerase; Agilent) flanking the neo cassette (conferring resistance to geneticin ...
-
bioRxiv - Cancer Biology 2022Quote: ... sgRNA sequence libraries were prepared from genomic DNA by two rounds of PCR using the Herculase II Fusion DNA Polymerase (Agilent). sgRNA sequences were amplified by the primers oMCB1562 and oMCB1563 and then indexed using the Illumina TruSeq LT adaptor sequences (AD002 ...
-
bioRxiv - Immunology 2022Quote: ... 60ng of DNA was used for PCR mix reaction prepared according to the manufacturer protocol (Herculase II Fusion DNA Polymerase, Agilent). The following Cre primers were used to carry out the PCR amplification ...
-
bioRxiv - Cell Biology 2022Quote: ... Guide sequence libraries were prepared from genomic DNA by two rounds of PCR using the Herculase II Fusion DNA Polymerase (Agilent). First ...
-
bioRxiv - Cancer Biology 2023Quote: ... ~100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
-
bioRxiv - Genetics 2021Quote: ... while the longer “plasmid” PCR amplicons were generated using Herculase II Fusion DNA Polymerase (Agilent). Primer sequences used for chimeragenesis are listed in Table S8.
-
bioRxiv - Genetics 2020Quote: ... and a pair of specific oligonucleotides (Table S1) and PfuUltra High-Fidelity DNA polymerase (Agilent).
-
bioRxiv - Evolutionary Biology 2020Quote: ... with the following composition: 0.5 μL Herculase II fusion DNA polymerase (Agilent, Santa Clara CA) per 50 μL reaction ...
-
bioRxiv - Molecular Biology 2021Quote: ... Site-directed mutagenesis was performed by PCR techniques using PfuUltra high-fidelity DNA Polymerase (Stratagene). All mutations were confirmed by DNA sequencing ...
-
bioRxiv - Microbiology 2020Quote: ... PCR was performed using the PfuUltra II Fusion High-fidelity DNA polymerase (Agilent, California, USA) using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen ...
-
bioRxiv - Microbiology 2019Quote: ... The RING C15/18A mutant of TRIM5 was generated using PfuTurbo DNA polymerase (Stratagene; 600250) with an annealing temperature of 55°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... with the exception of PfuUltra II Fusion HS DNA Polymerase that was procured from Stratagene, Sweden ...
-
bioRxiv - Biochemistry 2023Quote: ... PCR reactions were performed using a Pfu-Ultra II Fusion High Fidelity DNA Polymerase (Agilent). All sequences were verified using Sanger sequencing (Microsynth) ...
-
bioRxiv - Plant Biology 2023Quote: ... PCR amplification was carried out using Herculase II Fusion DNA Polymerase enzyme (Agilent Genomics, USA) with specific primers (Supplemental Table S1).
-
bioRxiv - Developmental Biology 2024Quote: ... sgRNAs were amplified and equipped with adapters using Herculase II Fusion DNA Polymerase (Agilent, #600677) for 25 cycles ...
-
bioRxiv - Neuroscience 2024Quote: ... PCR reactions were performed using a Pfu-Ultra II Fusion High Fidelity DNA Polymerase (Agilent), whereas Gibson Assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2024Quote: ... sgRNA was amplified from 50 mg gDNA using Herculase II Fusion DNA Polymerase (Agilent Technologies), column purified using Select-a-Size DNA Clean & Concentrator kit (Zymo Research) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 0.2 µl Pfu Polymerase (1x polymerase condition, Agilent), 0.5 µl forward primer (10 µM ...
-
bioRxiv - Neuroscience 2023Quote: ... the four 130 bp α-unit DNA fragments were amplified from each α-unit plasmid using the Herculase II Fusion DNA polymerase (Agilent) and oJS2581 and oJS2582 primers77 ...
-
bioRxiv - Genetics 2021Quote: ... Primers were used in a high fidelity PCR reaction using Pfu DNA polymerase obtained from Agilent® ...
-
bioRxiv - Molecular Biology 2021Quote: ... The fragment was amplified with the high fidelity Pfu Ultra II Fusion HS DNA polymerase (Agilent) according to the manufacturer’s recommendations using the primers ...
-
bioRxiv - Cell Biology 2021Quote: PCR of the insert was performed using PfuUltra II Fusion Hotstart DNA Polymerase (Agilent Technologies, #600672), following the manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2020Quote: ... 0.5 μl 10 mM dNTP mix and 0.5 μl Herculase II fusion DNA polymerase (Agilent Technologies). PCR parameters were as follows ...
-
bioRxiv - Genomics 2021Quote: ... Each aDNA library was double indexed66 in parallel 100 μl reactions using PfuTurbo DNA Polymerase (Agilent) (protocol ...
-
bioRxiv - Physiology 2021Quote: ... By PCR using PfuUltra II Fusion HS DNA Polymerase kit (Agilent technologies, Santa Clara, CA, USA) and the primers (Supplementary Table 1) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The conditions for PCR with high-fidelity Pfu DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) were empirically modified (denaturation at 94 °C for 20 s ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... High fidelity amplification was achieved using Herculase II Fusion DNA Polymerase (Agilent, Santa Clara, California, USA) and a touchdown thermal profile ...
-
bioRxiv - Genetics 2021Quote: ... an in-out PCR was performed with Herculase II Fusion High-Fidelity DNA Polymerase (Agilent Technologies). The specific size of the PCR products was verified in 1% agarose gel ...
-
bioRxiv - Plant Biology 2020Quote: ... Mutations were introduced into the CCT domain by site-directed mutagenesis (Pfu Turbo DNA polymerase, Agilent) and all the constructs were verified by sequencing and subsequently transformed into BL21-CodonPlus (DE3)-RIL Competent Cells for protein production.
-
bioRxiv - Microbiology 2022Quote: ... all clonings in the suicide vector pSW7848T were performed using Herculase II fusion DNA polymerase (Agilent) for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs ...
-
bioRxiv - Immunology 2022Quote: Synthesized cDNA was then subjected to SMART PCR using Herculase II fusion DNA polymerase (Agilent Technologies) with a common forward primer (5Anchor1-FW1 ...
-
bioRxiv - Bioengineering 2022Quote: The targeted loci were amplified from extracted genomic DNA by PCR using Herculase II polymerase (Agilent). PCR amplicons were sequenced using primers ∼200 bp from the expected cut site ...
-
bioRxiv - Bioengineering 2022Quote: The targeted loci were amplified from extracted genomic DNA by PCR using Herculase II polymerase (Agilent). The PCR primers included Illumina sequencing handles as well as replicate-specific barcodes ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.2 μM of each primer and 2.5 U of PfuTurbo Cx Hotstart DNA Polymerase (Agilent Technologies). Following completion of mutagenesis reactions ...
-
bioRxiv - Microbiology 2024Quote: ... pBRplac derivatives were mutagenized using PCR with mutagenic primers and the Pfu Turbo DNA polymerase (Agilent), followed by DpnI digestion of the template ...
-
bioRxiv - Physiology 2020Quote: ... The QuikChange II Site-Directed Mutagenesis protocol was performed using 200 ng of plasmid DNA and PfuTurbo DNA Polymerase according to the manufacturer’s protocol (Agilent Technologies, Santa Clara, CA). Mutagenesis reactions were then digested with Dpn I (New England Biolabs ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cDNA coding TALK1DN was generated by site-directed mutagenesis using PCR and PfuTurbo DNA polymerase (Agilent). The whole cDNAs were sequenced ...
-
bioRxiv - Neuroscience 2019Quote: ... The K186A mutant (AAG>GCG) was generated by site-directed mutagenesis employing PfuTurbo DNA polymerase (Agilent Technologies). All constructs were verified by DNA sequencing (Eurofins Genomics) ...
-
bioRxiv - Cell Biology 2022Quote: ... The UAS site deletion was generated performing single primer mutagenesis using Pfu Turbo Cx DNA polymerase (Agilent). The pBID-GmCherry vector was constructed by replacing the mVenus of the pBID-GV vector by the mCherry sequence ...
-
bioRxiv - Biochemistry 2021Quote: ... B35SSB mutants were obtained by direct mutagenesis using different oligonucleotides (Table S1) and Pfu DNA Polymerase (Agilent). The expression vectors were used to transform E ...
-
bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
-
bioRxiv - Microbiology 2020Quote: Polymerase chain reactions were performed using Pfu Ultra II polymerase (Agilent) or Clontech Hifi polymerase (Takara) ...