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Citations for Agilent :
301 - 350 of 598 citations for Crimean Congo Hemorrhagic Fever Virus Gn Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Ligand binding to protein was detected using DSF on an MX3005P qPCR system from Agilent Technologies as described elsewhere.37 Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The nsp14 and nsp10 proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Stratagene). The bacteria were grown in Luria–Bertani medium supplemented with 50 mg/mL kanamycin at 37 °C to an OD600 of 0.6 ...
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bioRxiv - Cancer Biology 2019Quote: ... sections were incubated for 5 min at room temperature with Protein Block Serum-Free (Dako). The tissue sections were then incubated with the anti-249T-P pAbs or TpMab-1 mAb (1 ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were expressed using E.coli BL21 (DE3) RIL according to the manufacturer’s protocol (Stratagene). The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs ...
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bioRxiv - Physiology 2019Quote: ... Slides were incubated with DAKO Protein Block Serum-Free (Agilent Technologies, Inc, Santa Clara, CA) at room temperature for 10 minutes and then with the primary antibodies diluted in Dako Antibody Diluent (Agilent Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were expressed in BL21-CodonPlus (DE3)-RIL Escherichia coli (Agilent Technologies, catalog no. 230245) at 37°C with 0.25 mM IPTG (Isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were washed and blocked for 10 min (Dako protein block; Agilent, Santa Clara, CA) before being treated with primary antibodies overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasma samples were depleted of high- and mid-abundant proteins using immune-depletion columns (Agilent Multiple Affinity Removal Column MARS3 (Agilent 5188-5218 ...
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bioRxiv - Plant Biology 2023Quote: ... Substrate proteins were induced and expressed in Escherichia coli BL21 CodonPlus(DE3)-RIL cells (Stratagene) over night at 18°C after induction with 1 mM IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used in this study were glial fibrillary acidic protein (GFAP; Source: Dako; Host ...
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bioRxiv - Immunology 2023Quote: ... VP protein masses were then measured using an in-line 6545XT QTOF system (Agilent Technologies) using the following parameters ...
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bioRxiv - Biochemistry 2023Quote: ... Secreted proteins were captured by addition of 100:1 (v/v) secretome:Strataclean resin (Agilent Technologies) (∼2 ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and treated for a further 10 min with serum-free protein block solution (Agilent; #X0909). Primary antibodies were diluted in antibody diluent (Agilent ...
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bioRxiv - Biophysics 2023Quote: Halo-tagged dCas9 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pET302-6His-dCas9-halo (Addgene #72269 ...
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bioRxiv - Cell Biology 2023Quote: For expression of recombinant proteins (listed in Table S1) we used ArcticExpress (DE3) bacteria (Agilent) or SF9-ESF S ...
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bioRxiv - Neuroscience 2023Quote: ... All other proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ overnight ...
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bioRxiv - Systems Biology 2024Quote: ... VA) or glial fibrillar acidic protein (GFAP; 1:1000; G3893-100uL; Dako, Santa Clara, CA). For Iba1 staining ...
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bioRxiv - Neuroscience 2023Quote: ... and 200 μL of blocking solution containing 50% serum-free protein block (X090930-2, Dako) and 50% normal horse serum (2.5% blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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bioRxiv - Cell Biology 2023Quote: Bsp1 proteins were expressed overnight at 20°C in BL21 pLysS Gold competent cells (Agilent) using autoinduction LB medium (Formedium) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4°C overnight with primary antibody in Protein Block (Dako, CA). Sections were rinsed 4x in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were visualized with 3,3′-diaminobenzidine (DAB) (K346811-2, Agilent Technologies, Inc., Santa Clara, CA) or Vina Green (BRR807AS ...
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bioRxiv - Cancer Biology 2024Quote: ... Each cycle included a 5 min blocking step with serum free Protein Block (Agilent, #X0909), primary antibody incubation for 30 min ...
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bioRxiv - Genomics 2021Quote: ... using a mixture of equal volume of ready-to-use protein block serum-free solution (Agilent Dako ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were submitted to the Stanford Protein and Nucleic Acid Facility for quality assessment by Agilent Bioanalyzer to determine the RNA integrity (RIN ...
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bioRxiv - Developmental Biology 2020Quote: ... the slides were further washed and blocked in protein block serum-free buffer (Dako, Cat # X0909). The slides were incubated with AIBP antibody for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... then stained with: rabbit polyclonal (pAb) glial fibrillary acidic protein (GFAP,1:1000, Dako, Z033401-2), goat pAb glial fibrillary acidic protein (GFAP,1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... The labeled proteins were subjected to digestion with Sialidase A (Agilent Technologies, Santa Clara, CA, USA), β1-4 galactosidase (Agilent Technologies) ...
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bioRxiv - Neuroscience 2019Quote: Primary antibodies used for immunofluorescence were polyclonal rabbit anti-glial fibrillary acidic protein antibody (GFAP; DAKO, Santa Clara ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation in 3% hydrogen peroxide and in serum-free protein block solution (Dako, X0909) before adding primary antibody for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The molecular weights for each rNA were estimated using an AdvanceBio SEC 300Å protein standard (Agilent) of known molecular weights that was included in the run and the presence of NA in each fraction was measured with the Mu-NANA assay.
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bioRxiv - Microbiology 2021Quote: ... Separation of digested protein samples was performed on an Agilent 1290 Infinity HPLC system (Agilent Technologies). Samples were loaded on a 100 µm × 20 mm trap column (in-house packed with ReproSil Pur C18-AQ ...
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Long-term alterations in brain and behavior after postnatal Zika virus infections in infant macaquesbioRxiv - Neuroscience 2019Quote: ... we performed immunohistochemical (IHC) analysis by using antibodies against Glial fibrillary acidic protein (Agilent Technologies, M0761), NeuN (EMD Millipore ...
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bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
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bioRxiv - Biochemistry 2022Quote: ... 100 µg of anti-HA antibody (12CA5) was immobilized on Protein A cartridges (Agilent, G5496-60000), using the in-built immobilization methodology ...
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bioRxiv - Microbiology 2021Quote: ... the protein was digested by trypsin overnight and the digested peptides were purified with ZipTips (Agilent). Using a Dionex UltiMate 3000 RSLCnano system equipped with a Dionex UltiMate 3000 RS autosampler ...
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bioRxiv - Neuroscience 2020Quote: ... Peptide/protein identifications and quantitative analysis were performed using Spectrum Mill (Agilent Technologies, Santa Clara, CA) as described previously ...
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bioRxiv - Cancer Biology 2022Quote: ... 1mL fractions were collected and protein was concentrated by incubation with 4μL StrataClean resin (Agilent Technologies) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and slides were incubated with Dako Serum Free Protein Block (#X090930-2, Agilent, Santa Clara, CA) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... FcγR binding was quantified by incubating immune complexes with biotinylated FcγRs (FcγRIIB, FcγRIII, and FcγRIV, courtesy of Duke Protein Production Facility) conjugated to Steptavidin-PE (Prozyme). Flow cytometry was performed with an IQue (Intellicyt ...
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bioRxiv - Neuroscience 2020Quote: ... All recombinant proteins were expressed in the BL21-Gold (DE3) pLysS strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase (GST ...
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bioRxiv - Biophysics 2021Quote: ... Separation of digested protein samples was performed on an Agilent 1290 Infinity HPLC system (Agilent Technologies). Samples were loaded on a 100 µm × 20 mm trap column (in-house packed with ReproSil-Pur C18-AQ ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigens (for HE1-, or LL-) proteins were detected using 10 mg tablets of DAB (Dako Cytomation) diluted in 5 ml Tris-buffer (brown colour).
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bioRxiv - Neuroscience 2022Quote: ... washed in PBS (3 × 5min) and incubated with serum free protein block (Dako, Cat# X090930-2) for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-glial fibrillary acidic protein (GFAP) antibody (RRID:AB_10013382; Dako Agilent, Santa Clara, CA, USA, #z0334), mouse anti-DCX (RRID:AB_10610966 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-glial fibrillary acidic protein (GFAP) antibody (RRID:AB_10013382; Dako Agilent, Santa Clara, CA, USA, #z0334), mouse anti-DCX (RRID:AB_10610966 ...
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bioRxiv - Physiology 2023Quote: ... Slides were blocked with serum free protein block (Dako X0909; Agilent Technologies; Santa Clara, CA, USA). Insulin was labeled with primary guinea pig anti-insulin antibody (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Data were normalized to urea lysate protein levels and analyzed using Wave Desktop Software (Seahorse Bioscience), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After washing with PBS the aortic tissue sections were specifically stained with primary antibodies for platelet GPIbα (CD42b #M042-0 ...