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Citations for Agilent :
301 - 350 of 8096 citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... was generated by site directed mutagenesis using primers oM18_17 and oM18_18 (Table 1) and a Quikchange II Site-directed Mutagenesis kit (Agilent, 200523). The PCR product was digested with DpnI enzyme to remove template vector and transformed into Stbl2 chemically competent cells (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... and cDNA library size was checked using Fragment Analyzer HS NGS Fragment Kit (1-6000bp) (Agilent formerly Advanced Analytical). Only high-quality cDNA libraries without notable degradation were used for sequencing library construction ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation variants found in P.1 and 10-mutation variant (BZΔ10) were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana VSV (rVSV ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The Telomere PNA Kit/FITC kit from Dako (Agilent) was used to estimate Telomere length signal in samples ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Microbiology 2019Quote: ... The resulting products were then run with a Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Quantification was performed using the following equation ...
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bioRxiv - Biochemistry 2020Quote: ... fulgidus Csx3 (Supplementary figure 1) were generated using the QuikChange Site-Directed Mutagenesis kit as per manufacturer’s instructions (Agilent Technologies) and purified as for the wild-type protein.
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bioRxiv - Microbiology 2020Quote: ... an optimal mutation rate (0.3–1 base/kb) for 1µg of template was adopted as recommended in GeneMorph II Random Mutagenesis kit (Agilent Technologies). The PCR products were then digested with EcoRI and HindIII and ligated into the pJF118EH vector using the same restriction enzymes ...
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bioRxiv - Molecular Biology 2020Quote: ... were introduced using RE specific primers (Table 1 and Table 2 in supplementary material) by QuickChange Multi site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... An AgeI site was inserted into exon 1 of Cacna1b by mutagenesis using a QuickChange II XL site-directed mutagenesis kit (Stratagene) with primers 1-For and 1-Rev ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then ran the digested products on 1% agarose gel and extracted the desired fragments using StrataPrep DNA Gel Extraction Kit (Agilent). We then assembled these two fragments using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... the relevant tetrapeptide motifs were inserted into iBox-PAK4cat within the pXJ40 vector [1] using the Quikchange II Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The prepared library was quantified using Qubit and validated for quality by running an aliquot (1 μl) on High Sensitivity DNA Kit (Agilent) which showed expected fragment distribution in the range of ~250–500 bp ...
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bioRxiv - Microbiology 2021Quote: Mutations were introduced into the HA plasmid of A/Hong Kong/1/1968 using a site-directed mutagenesis kit (QuikChange; Stratagene). 2:6 recombinant IAVs containing wt and modified HA of A/Hong Kong/1/1968 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cy3-labelled probes were prepared from RNA using Low Input Quick Amp Labeling Kit 1-color (Agilent Technologies, #5190-2305) and hybridized with the custom microarrays (ID:085740 ...
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bioRxiv - Genomics 2021Quote: ... The shape and the mean fragment size of the libraries were determined on a 5200 Fragment Analyzer using the HS NGS Fragment Kit 1-6000 bp (both Agilent). Enriched libraries were loaded with a final concentration of 10 pM on a MiSeq Flow Cell using v3 reagent chemistry for 2×150 cycles.
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bioRxiv - Biochemistry 2021Quote: ... Cys to Ala mutants of Flag-tagged DJ-1 were using QuikChange Ⅱ Site-Directed Mutagenesis kit (Agilent Technologies, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was carried out with the LSAB2 System-HRP (anti-rabbit/mouse LSAB2 Kit Dako Cat#K0679, dilution 1:100) or the VECTASTAIN Elite ABC HRP Kit (Vector Labs ...
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bioRxiv - Genetics 2023Quote: ... the Prab-3p::hpk-1 plasmid was used and mutations were performed with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All the assembled plasmids were validated by sequencing prior to microinjection ...
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bioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent).
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bioRxiv - Immunology 2023Quote: ... and Seahorse XF Cell Mito Stress Test Kit (catalog number 103015-100, contains 1 each of oligomycin, FCCP, and rotenone/antimycin A.) were all purchased from Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Library size was validated by using Fragment Analyzer 48-Capillary Array with the HS NGS Fragment Kit (1-6000bp) (Agilent). Libraries were sequenced with a Nextseq 2×150 pb paired-end reagent kit (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Plant Biology 2020Quote: ... protein concentrated from the liquid media with Strataclean resin (Agilent Technologies) after filtration was used as an extracellular fraction.
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bioRxiv - Cancer Biology 2021Quote: ... the sections were blocked with Protein block Serum free DAKO (Agilent) for 30 to 45 min at RT ...
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bioRxiv - Physiology 2021Quote: ... and serum-free protein block (X090930-2) were from Dako (Agilent). Rabbit anti-Ki67 mAb D3B5 (9129 ...
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bioRxiv - Cancer Biology 2020Quote: ... and an additional serum free protein block (Dako Cytomation, Carpinteria, CA) using an automated system (Dako Cytomation ...
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bioRxiv - Immunology 2021Quote: ... Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein was purchased from Agilent Dako (Z033401-2) ...
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bioRxiv - Genomics 2020Quote: ... Slides then incubated with serum-free protein block (Agilent X090930-2) for 30 mins as an extra blocking step in order to prevent nonspecific antibody binding ...
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bioRxiv - Cell Biology 2020Quote: ... the protein stored in denatured buffer was desalted with HPLC (Agilent) and freeze-dried by the FreeZone lyophilizer (Thermo Fisher) ...
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bioRxiv - Biochemistry 2021Quote: Proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL (Stratagene) in Luria-Bertani (LB ...
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bioRxiv - Biochemistry 2021Quote: ... Fusion proteins were expressed and purified from BL21 Codon RIL (Stratagene). Expression was induced by adding 0.15 mM IPTG for 4,5 h at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... pre-incubated with Protein Block Serum-Free (X0909, Dako, Jena, Germany) for 30 min at room temperature and then exposed to primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... we immuno-stained for glial fibrillary acidic protein or GFAP (Agilent Technologies ...
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bioRxiv - Biophysics 2021Quote: ... All protein mutants were generated using the QuikChange protocol from Stratagene and obtained following the expression and purification protocols described above ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was detected with 280 nm UV light (Agilent Technology 1260), a quasielastic light scattering module (DAWN-8+ ...
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bioRxiv - Physiology 2022Quote: ... The sections were then blocked with serum-free protein block (Dako) for 10 mins before anti-vimentin antibody was applied to slides ...
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bioRxiv - Pathology 2021Quote: ... blocked with DAKO Protein Block Serum-Free (Dako, Carpinteria, CA, USA), and incubated with anti-human Spred2 polyclonal antibody (Proteintech ...
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bioRxiv - Biochemistry 2020Quote: ... Fusion protein was expressed in E.coli BL21(DE3) CodonPlus-RIL (Stratagene) in Super Broth Auto Induction Media (Formedium ...
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bioRxiv - Biochemistry 2020Quote: ... was immobilized on Protein A (PA-W) cartridges (Agilent, G5496-60000), using the Immobilization App (Agilent Sample Prep Workbench v3.0.0) ...
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bioRxiv - Microbiology 2021Quote: ... Insoluble proteins were purified with StrataClean beads (Agilent, Santa Clara, USA).
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked in a serum-free protein block (X0909, Agilent) for 10 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... pre-incubated with Protein Block Serum-Free (X0909, Dako, Jena, Germany) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein (10 μL) was loaded by an LC system (Agilent 1290 Infinity II ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then blocked with serum-free protein block solution (Dako) and incubated with corresponding primary antibodies at 4°C overnight ...