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Citations for Agilent :
301 - 350 of 4970 citations for Cell Division Cycle 2 Like Protein Kinase 6 CDC2L6 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... bEnd.3 cells were seeded at a density of 6×103 cells/well in rat tail collagen-coated (150 µg/mL) 96-well plates (E-plate 96; Agilent, cat# 300600910) and confluent monolayers were treated as described above for human ECs ...
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bioRxiv - Bioengineering 2023Quote: ... rat ECs were seeded at a density of 6×103 cells/well in 96-well plates (E-plate 96; Agilent, cat# 300600910) coated with collagen IV (100 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with HRP-conjugated rabbit anti-mouse antibody (DaKo, P0161) diluted 1:5000 in blocking buffer for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were stained with antibodies: Guinea pig anti-insulin (1:100, Dako) followed by anti-guinea pig-Alexa Fluor® 555 (1:100 ...
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bioRxiv - Pathology 2023Quote: ... cells were incubated in anti-BrdU primary monoclonal antibody (DAKO, Glostrup, Denmark), and fluorescein isothiocyanate-conjugated anti-mouse secondary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... Accuscript reverse transcriptase (6×10−5) (Agilent, product literature), and Phusion DNA polymerase after 20 cycles of amplification (1.2×10−5 ...
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bioRxiv - Microbiology 2019Quote: ... GFAP (Dako #M076101-2 or #Z033429-2), OLIG2 (R&D Systems #AF2418 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The multiple charged peaks of the antibody were deconvoluted using the Agilent MassHunter Bioconfirm software (deconvolution for protein, Agilent technology) with the deconvolution range from 20 kDa to 160 kDa ...
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bioRxiv - Bioengineering 2019Quote: ... The primary antibodies sources and dilutions were as follows: rabbit poly-clonal anti-glia fibrillary acidic protein (GFAP; 1:500; DAKO), mouse monoclonal anti-Neuronal Class III B-Tubulin (TuJ1 ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were incubated overnight with the following primary antibodies: rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:6,000, DAKO, UK), rabbit polyclonal anti-allograft inflammatory factor 1 (IBA1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were probed with a set of 216 antibodies against total and phospho-proteins using an automated slide stainer Autolink 48 (Dako). Each slide was incubated with one specific primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and 10% Methanol for 10 min and incubated overnight with the primary antibody (Table 2) in PBS/T 0.1 with 10 % normal swine/ goat or rabbit serum (Dako). Second ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were incubated for 1 hour at room temperature with serum-free protein block (Dako X0909) to block non-specific binding sites followed by Cleaved-Casp3 primary antibody (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Cells (1-2 × 105) were plated in Poly-D-Lysine coated 96-well microplates (Agilent) with 4-5 technical replicates ...
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bioRxiv - Genomics 2020Quote: RNA quality was reassessed after a freeze thaw cycle using the Bioanalyzer RNA 6000 pico chip (Agilent, 5067-1513) and quantity was assessed using the Qubit RNA Broad Range Assay (Invitrogen ...
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bioRxiv - Physiology 2019Quote: ... Final libraries were amplified for 14 cycles by PCR and the final size assessed using a 2100 bioanalyzer (Agilent) with a HS DNA chip (Agilent) ...
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bioRxiv - Genetics 2020Quote: ... Sequencing libraries were prepared by PCR amplification (30 cycles, annealing temperature 55C) using PfuUltra II Fusion DNA polymerase (Agilent) and custom primers that were designed to anneal to a 3’ site within the EGFP gene (F ...
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bioRxiv - Genomics 2019Quote: ... All other pools were designed to allow for amplification in 15 cycles using Herculase II DNA polymerase (Agilent Technologies) with forward primer pool-FP2 (or pool-FP3 ...
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bioRxiv - Bioengineering 2023Quote: ... protein block with Dako protein block solution (Dako, Glostrup, Denmark), incubation with primary antibodies [eGFP (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... protein block (Dako) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Protein Block (Agilent) was used to block nonspecific antibody binding before incubating the sections with primary antibody overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies). Lager scale transient transfections were purified on the Akta FPLC system using 1mL HiTrap MabSelect PrismA™ affinity chromatography resin (Cytiva) ...
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bioRxiv - Pathology 2024Quote: ... sections were incubated overnight at 4 ⁰C with a cocktail of the primary antibodies αSyn (clone KM51 1:500, Monosan Xtra, The Netherlands) and glial fibrillary acidic protein (GFAP, Z0334, 1:4000, DAKO, Denmark). After rinsing in PBS ...
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bioRxiv - Neuroscience 2024Quote: Mouse hind paws were fixed in Zamboni’s fixative for 24-48 hours at 4°C and processed as previously described.44 50 μm floating tissue sections were stained with the primary antibody rabbit anti-protein-gene-product 9.5 (PGP 9.5) (Dako, Z5116, 1:500) overnight and the secondary biotinylated goat anti-rabbit IgG antibody (Vector Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... HEK-293T cells were co-transfected with the following plasmids: pAAV/L7-6-Slc9A6-GFP or pAAV/L7-6-GFP (control) with pHelper (Stratagene, La Jolla, CA, USA), and pUCmini-iCAP-PHP.eB followed by viral particles purification ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated with anti-VP1 antibodies (Dako, anti-enterovirus clone5-D8/1) diluted 1:500 in blocking solution for detection of viral protein VP1 or anti dsRNA antibody (SCICONS ...
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bioRxiv - Physiology 2019Quote: ... and analyzed by Western blotting using the specific first antibodies listed in Supplementary Table 2 with goat anti-rabbit and goat anti-mouse peroxidase-conjugated immunoglobulin as secondary antibodies (Dako). The blots were then processed with the luminescence technique ECL (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... sections were incubated for 2 hours at room temperature with polyclonal guinea pig anti-insulin antibody (1:5, Agilent Technologies). Slides were then washed 3 x 5 minutes with PBS and incubated with the Anti-guinea pig IgG Alexa Fluor 488 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were then washed twice with TBS-T followed by incubation for 1 h at room temperature in immunoreaction enhancer solution 2 (Can Get Signal; TOYOBO) with peroxidase-conjugated antibody against rabbit IgG (Dako) as the secondary antibody ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2 hours at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope.
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bioRxiv - Microbiology 2021Quote: Immunofluorescent (IF) staining was performed using the following primary antibodies: polyclonal rabbit anti-HSV-1 (cross-reactive with HSV-2; Agilent), anti-Iba1 (Wako ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were permeabilized with 0.2% Triton X-100 in 2% fish gel for 2 hours at room temperature and immunohistochemically labelled with the primary antibody (1:200 rabbit anti-GFAP, Z0334 Dako; 1:200 rabbit anti-Iba1 ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal goat anti-rabbit secondary antibody IgG/HRP (P044801-2; Agilent Technologies/Dako ...
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bioRxiv - Cell Biology 2024Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2 hr at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope ...
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bioRxiv - Developmental Biology 2020Quote: ... Guinea Pig anti-Insulin pre-diluted 1:6 (Dako 1R002), Chicken anti-GFP 1:1000 (Abcam ab13970) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... low pH∼6 (Dako, K8005; Agilent, Santa Clara, CA, USA), with preheating to 80°C and increased temperature to 95°C for 20min after slides were added ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... low pH∼6 (Dako, K8005; Agilent, Santa Clara, CA, USA), with preheating to 80°C and increased temperature to 95°C for 20min after slides were added ...
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bioRxiv - Cancer Biology 2021Quote: ... cytokeratin 5/6 (mouse monoclonal, clone 6D5/16 B4, Dako), epidermal growth factor receptor (EGFR ...
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bioRxiv - Biochemistry 2020Quote: Skd3 variants were expressed as an N-terminally MBP-tagged protein in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in 40mM HEPES-KOH pH=7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... cells or tissue sections were incubated with Protein Block Serum-Free Ready-To-Use (Dako, #X 0909) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... A53T and E46K α-synuclein proteins were expressed in BL2-Gold(DE3) competent cells (Agilent, Cat #230132). Protein expression was induced at log phase with 200 μM IPTG for 16 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Protein expression was conducted in E.coli BL21 (DE3) Codon Plus cells (Agilent Technologies, San Diego, CA, USA). Induced cultures were grown at 18° C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... 15 PCR cycles were used for final library amplification which was analyzed on a Bioanalyzer using a high sensitivity DNA chip (Agilent). ChIP-seq libraries were single-end sequenced for 50bp using an Illumina NextSeq platform (Cold Spring Harbor Genome Center ...
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bioRxiv - Cancer Biology 2019Quote: ... 15 PCR cycles were used for final library amplification that was analyzed on a Bioanalyzer using a high sensitivity DNA chip (Agilent). ChIP-seq libraries were single-end sequenced for 50bp using an Illumina NextSeq platform (Cold Spring Harbor Genome Center ...