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Citations for Agilent :
151 - 200 of 2030 citations for Calcium Channel Voltage Dependent L Type Alpha 1C Subunit CACNA1C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... All antibodies were diluted in Dako Antibody Diluent (Dako Antibody Diluent S0809; Agilent Technologies). Images were captured by confocal microscopy (Leica DMi8 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in antibody diluent (Dako) and applied for 45 minutes at room temp ...
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bioRxiv - Physiology 2022Quote: ... Primary antibodies were diluted in antibody diluent (Dako) and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in Antibody Diluent (Dako) and a droplet of 25 µl or 50 µl applied on the sample for an 8-well or a coverslip upside down ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were diluted in Antibody Diluent (Dako) and incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies were diluted in antibody diluent (Dako, S0809) containing 0.3% Triton X-100 and incubated overnight at 4 °C in a humidified chamber ...
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bioRxiv - Neuroscience 2023Quote: ... XF L-Glutamine and cells were prepared for the Mito Stress Assay (Agilent) as per manufacture’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... or 2 mM L-glutamine for the Glycolysis Stress Test (Agilent #103020-100). After an hour incubation at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The H335A mutation that renders PLC-γ1 catalytically inactive was introduced into the transfer vector encoding wild-type PLC-γ1 (residues 21-1215) or PLC-γ1 (D1165H) using standard primer-mediated mutagenesis (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: Desired mutations were introduced into mhsT wild-type gene via site-directed mutagenesis (Mutagenesis QuikChange Lightning Kit, Agilent Technolgies, Inc) using designed primers ...
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bioRxiv - Bioengineering 2019Quote: Variant CvFAPG462V was generated by site-directed mutagenesis of the wild-type construct in pETM11 using the QuikChange whole plasmid synthesis protocol (Stratagene) with CloneAmp HiFi PCR premix (Clontech) ...
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bioRxiv - Biochemistry 2021Quote: ... RpoB E310A and RpoB E310K were made by site-directed mutagenesis of the wild type constructs using the Quikchcange II XL kit (Agilent). B ...
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bioRxiv - Biochemistry 2020Quote: The FANCD2 S592A and S592D cDNAs were generated by site-directed mutagenesis of the wild type FANCD2 cDNA using the Quikchange Site-directed Mutagenesis Kit (Stratagene). The forward (FP ...
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bioRxiv - Biochemistry 2020Quote: ... The gene coding for the H349Y GlgE variant was generated from the synthesized wild-type glgE gene using a QuikChange Lightening kit (Agilent). The constructs were ligated into pET21a expression vectors (Novagen ...
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bioRxiv - Biochemistry 2020Quote: The modified pVote0GW vector used for wild-type hTop2α overexpression was mutated by site directed mutagenesis using the QuikChange XL Site-Directed Mutagenesis kit (Agilent) in order to generate the plasmids harboring K418A ...
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bioRxiv - Cell Biology 2019Quote: ... The Lys162Met variant for all AURKA biosensors analysed in the study were obtained from the corresponding wild-type constructs by QuikChange site-directed mutagenesis (Stratagene) with the following primers ...
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bioRxiv - Developmental Biology 2019Quote: ... was created by introducing the point mutation in wild-type vasa cDNA sequence by site directed mutagenesis using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). The transgene is subsequently cloned downstream of GFP into the pUASK10attB vector (gift of Beat Suter) ...
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bioRxiv - Developmental Biology 2019Quote: ... pBMN-HIPK4-Y175F-3xFLAG-IRES-mCherry was later generated by site-directed mutagenesis of the wild type construct using the primers 5’-CGCTATGTGAAGGAGCCTTTCATCCAGTCCCGCTTC TAC-3’ and 5’-GTAGAA GCGGGACTGGATGAAAGGCTCCTTCACATAGCG-3’ and PfuUltra II Fusion polymerase (Agilent).
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bioRxiv - Microbiology 2021Quote: ... D950N) were prepared from wild-type SARS-CoV-2 spike using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: ... Parkin mutant plasmids were cloned beginning with a Homo sapiens wild type eGFP-parkin vector [36] and performing PCR point mutagenesis (Quikchange Mutagenesis kit: Stratagene) utilizing primers ordered from Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: Cys to Ala substitution mutations were introduced at specific sites in the ectodomain of wild type toll by using QuickChange II XL site-directed mutagenesis kit (Stratagene) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... These genes were cloned into an Adenovirus type 5 replication-defective E1/E3 deleted vector using the Ad-Easy Adenoviral Vector System (Agilent). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... All K-Ras secondary mutant and wild-type K-Ras constructs were generated by a PCR based strategy using a site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The C211G mutant was generated by site-directed mutagenesis of the wild-type vector using the QuikChange II protocol (Agilent). To generate the Myc-tagged versions ...
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bioRxiv - Biophysics 2023Quote: ... A clone encoding tagless wild type PUS1 was then generated from PUS1D134A by converting alanine 134 back to wild type aspartate 134 using a QuickChange II XL kit and protocol (Agilent).
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bioRxiv - Bioengineering 2023Quote: ... The library of CjCas9 variants was produced by random mutagenesis of the wild-type sequence using error-prone PCR approach (GeneMorph II kit from Agilent) (for primers ...
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bioRxiv - Molecular Biology 2023Quote: ... mutant plasmids encoding the different RNA exosomopathy amino acid substitutions were generated by site-directed mutagenesis of the respective wild-type plasmids (pAC3656, pAC3652, pAC3482) using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the desired missense mutations as previously described [33 ...
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bioRxiv - Cell Biology 2023Quote: Basal oxygen consumption rates in kidneys from non-diabetic and diabetic wild-type and Angptl4-/- mice were characterized using the Seahorse Flux Analyzer (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Extracted metabolites were separated on a Cogent Diamond Hydride Type C column of 1200 liquid chromatography coupled to an Mass 6220 TOF spectrophotometer (Agilent). Ion abundances of metabolites were estimated using Profinder 8.0 and log2 fold changes were analyzed and plotted using Gene Cluster 3.0 and Java Treeview.
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bioRxiv - Biochemistry 2023Quote: Oxygen consumption rates (OCR) of wild-type C2C12 under various concentrations of uncouplers were measured with Seahorse XFe24 analyzer (Agilent). Experiments were conducted under the same process as explained above ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies were diluted in antibody diluent (Dako, S302281-2). Nuclei were visualized by DAPI (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... All antibodies were prepared in antibody diluent from Dako envision kit ...
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bioRxiv - Immunology 2022Quote: ... Antibodies were diluted in Background Reducing Antibody Diluent (Agilent). The tissue was subsequently incubated with an anti-rabbit HRP polymer (Leica ...
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bioRxiv - Bioengineering 2021Quote: ... All antibodies were diluted in Dako Antibody Diluent (Dako Antibody Diluent S0809 ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were diluted in Background Reducing Antibody Diluent (Agilent). The tissue was subsequently incubated with an anti-rabbit HRP polymer (Leica ...
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bioRxiv - Microbiology 2022Quote: ... primary antibodies and appropriate HRP-conjugated secondary antibodies (DAKO). A chemiluminescence substrate (West Dura ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were diluted in antibody diluent (Agilent; #S3022) and incubated with sections for 16 hours at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies diluted in antibody diluent (S080983-2, Dako) were applied on sections for overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... antibodies were diluted in Background Reducing Antibody Diluent (Agilent) and subsequently incubated with an anti-rabbit HRP polymer (Leica ...
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bioRxiv - Biochemistry 2020Quote: ... Daily MS calibration was performed with ESI-L Low Concentration Tuning Mix (Agilent Technologies). The internal calibrant was Hexakis(1H,1H,3H-tetrafluoropropoxy)phosphazene (Synquest Laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... The mass spectrometer was calibrated in negative mode using ESI-L solution from Agilent technologies every 6 hours to maintain the best possible mass accuracy ...
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bioRxiv - Microbiology 2024Quote: ... The mass spectrometer was calibrated in negative mode using ESI-L solution from Agilent technologies every 6 h to maintain the best possible mass accuracy ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA quality and concentration of wild-type and mutant were determined using the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Total RNA (~1 μg ...
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bioRxiv - Biochemistry 2021Quote: ... and PcrA V448C were generated by site-directed mutagenesis of the wild type construct using the Quikchange II XL kit (Agilent Technologies). In the last gel filtration chromatography of PcrA V448C ...
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
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bioRxiv - Biophysics 2021Quote: ... An expression construct for R80A ParB was generated by site-directed mutagenesis of the wild type expression plasmid (QuikChangeII XL, Agilent Technologies). The mutant protein was expressed and purified in the same manner as wild type ...
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bioRxiv - Biochemistry 2022Quote: The L26P mutation was introduced into a wild-type AR sequence (pDONR221-AR-AD-WT) using a Quickchange™ protocol with Pfu Turbo polymerase (Agilent) and the following primer pairs:
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bioRxiv - Biophysics 2019Quote: All plasmids for expression of MBP-5-HT3A-ICD fusion constructs with wild-type or engineered ICD were transformed into Escherichia coli (E. coli) BL21-CodonPlus-(DE3)-RIPL cells (Agilent Technologies). Cells were grown in Terrific Broth (TB ...
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bioRxiv - Biochemistry 2022Quote: ... A398P and T435P mutations were introduced step-wise into a wild-type AR sequence (pDONR221-AR-AD-WT) using a Quickchange™ protocol with Pfu Turbo polymerase (Agilent) and the following primer pairs:
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bioRxiv - Biochemistry 2022Quote: ... C238P mutations were introduced step-wise into a wild-type AR sequence encoded in pDONR221-AR-AD-WT using a Quickchange™ protocol with Pfu Turbo polymerase (Agilent) and the following primer pairs: