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Citations for Agilent :
251 - 300 of 313 citations for Brucella Abortus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Biopsy sections were deparaffinized and antigens were retrieved by boiling in 10mM sodium citrate buffer pH6 for 40 minutes followed by endogenous biotin blocking (Agilent) and normal goat serum blocking (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were thoroughly washed with 0.3% Triton-TBS followed by antigen retrieval with target retrieval solution citrate pH6 (1:10, Dako S2031) for 20 minutes at 80°C ...
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bioRxiv - Zoology 2020Quote: ... Slides were rinsed with PBS (pH = 7.4) and then subjected to antigen retrieval procedure using proteinase K 1:30 (Dako, Denmark). Nonspecific binding sites were blocked with 2% BSA for 30 min in a humid chamber at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Antigen retrieval was performed either with Proteinase K (for F4/80) or boiling the sections in a high pH antigen retrieval buffer (Dako Target retrieval Solution ...
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bioRxiv - Microbiology 2021Quote: ... Specific antigen-antibody reactions were visualized by means of 3,3’-diaminobenzidine tetrahydrochloride staining using the Dako Envision system (Dako Cytomation).
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bioRxiv - Immunology 2019Quote: ... Epithelial cells in the EDTA fraction were depleted by incubation with anti-human epithelial antigen antibody (clone Ber-EP4, Dako) followed by anti-mouse IgG dynabeads (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were subjected to staining with F4/80 and CSF1R antibodies (cycle zero, no antigen retrieval, Table S3) and hematoxylin staining (S3301, Dako) for 1-5mins followed by whole slide scanning ...
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bioRxiv - Cancer Biology 2022Quote: ... and antigen retrieval was performed by heat-induced epitope retrieval (HIER) in Dako PT Link with a high pH buffer (Dako). The staining was performed with an autostainer (Autostainer Link 48 ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-GFAP immunohistochemistry (Polyclonal Rabbit Anti-Human, Proteinase K enzymatic antigen retrieval, dilution 1:1000, DAKO Omnis, Code Number: GA524) was employed to assess reactive astrogliosis ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were subjected to heat-induced antigen retrieval by incubation in a low pH buffer (Envision Flex TRS low pH (DAKO) for 20 min at 97°C (PT-Link ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were de-paraffinized using a graded alcohol series and antigen retrieval was carried out using target retrieval solution (Dako) at pH 6 or pH 9 in a pressure cooker ...
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bioRxiv - Cancer Biology 2023Quote: ... The heat-induced antigen retrieval was performed with a microwave oven or a pressure cooker in a citrate buffer solution (Dako). Histochemical stainings were carried out using standard techniques for IHC and IHC-IF ...
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bioRxiv - Immunology 2023Quote: ... Heat-activated antigen retrieval was carried out on rehydrated tissue sections by incubating slides in prewarmed Agilent Dako pH6 antigen retrieval solution (catalog no. S2367; Agilent) for 20-minutes in a vegetable steamer (Black &Decker) ...
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bioRxiv - Microbiology 2023Quote: ... Specific antigen-antibody reactions were visualized by means of 3,3’-diaminobenzidine tetrahydrochloride staining using the Dako Envision system (Dako Cytomation). Histopathological severity score of pneumonia was determined based on the percentage of alveolar inflammation in a given area of a pulmonary section collected from each animal in each group using the following scoring system ...
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bioRxiv - Microbiology 2023Quote: ... were prepared and stained with hematoxylin eosin (HE) for histological examination or subjected to immunohistological staining to detect SARS-CoV-2 antigen (performed in an autostainer; Agilent), using the horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2023Quote: ... Comparator an d frozen shoulder capsular tissue sections were obtained through deparaffinization and target retrieval steps (high pH, heat-mediated antigen retrieval) using an automated PT Link (Dako). Antibody staining was performed using the EnVision FLEX visualization system with an Autostainer Link 48 (Dako) ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was first performed with high or low pH buffer depending on the primary antibody (CC1m, Roche or low pH antigen retrieval buffer, Dako), endogenous peroxidase was blocked (peroxide hydrogen at 3% ...
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bioRxiv - Developmental Biology 2023Quote: For IHC staining slides were thawed and antigen-retrieval performed by incubating the sections with preheated Target Retrieval Solution (DAKO) 20 min at 70°C ...
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bioRxiv - Cell Biology 2022Quote: ... Immediately after the slides were immersed in pre-heated (90-100 degree Celsius) 1X antigen retrieval solution (Dako, cat # S1699) for 25 min ...
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bioRxiv - Cancer Biology 2023Quote: The 4-6um thick TMA sections were deparaffinized and antigens were unmasked using EnVision FLEX Target Retrival Solution Low pH (Dako Agilent) in PT link device (PT200 ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-µm sections were cut and antigen retrieval was carried out by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies) for Ki67 ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Brain sections that were stained with antibodies that required antigen retrieval were incubated in Dako 1X target retrieval solution (Agilent Dako) or sodium acetate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Heat antigen retrieval was performed by steaming at 98°C in target retrieval solution pH 6.1 (Dako, Carpinteira, CA, USA #S1699) for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... heat mediated antigen retrieval was performed for 3 min at 125°C in citrate pH 6.0 target retrieval solution (Dako Cat# S2369) using a decloaking chamber (Biocare Medical) ...
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bioRxiv - Cancer Biology 2022Quote: Formalin-fixed samples tissue microarrays of EwS-samples and normal tissues were stained for GPR64 after antigen retrieval using Target Retrieval Solution (Fa.Agilent Technologies, S1699) with anti-GPR64 (purified using Mouse TCS Antibody Purification Kit (ab128749 ...
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bioRxiv - Neuroscience 2021Quote: ... 3 μm paraffin-embedded tissue sections were either dewaxed and subjected to antigen retrieval treatment with Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent) or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005 ...
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bioRxiv - Neuroscience 2021Quote: ... or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005, Dako-Agilent) for 20 min at 97°C using a PT Link (Dako – Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 was detected using a rabbit polyclonal anti-hACE2 antibody (Cell Signalling, 4355S; citrate antigen-retrieval) and EnVision+ anti-rabbit HRP (Agilent, K4003). Slides were scanned with a bright field slide scanner (Leica ...
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bioRxiv - Immunology 2019Quote: ... Antigen retrieval was performed either with Proteinase K (for F4/80) or boiling the sections in a high pH antigen retrieval buffer (Dako Target retrieval Solution, pH 9, Dako, Denmark) (for Podocalyxin ...
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bioRxiv - Immunology 2021Quote: ... Slides were then rinsed with double distilled water (ddH2O) and boiled in 1X Dako pH9 antigen retrieval solution (Agilent, S236784-2) for 10 min ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed on 4-μm thick formalin-fixed paraffin-embedded (FFPE) whole tissue serial sections following antigen retrieval (Target Retrieval Solution, pH 6.1; Dako, Carpinteria, CA). Immunostains performed included Connexin 26 (Cx26 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Biophysics 2022Quote: ... Hela CD95KO and Hela WT stable CD95-mEGFP was assessed using the QIFIKIT® for quantification of cell surface antigens by flow cytometry (K007811-8, Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: The 4-6um thick TMA sections were deparaffinized and antigens were unmasked using EnVision FLEX Target Retrival Solution Low pH (Dako Agilent) in PT link device (PT200 ...
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bioRxiv - Molecular Biology 2024Quote: ... paraffin sections were rehydrated through graded alcohols and antigen retrieval was performed using Target Retrieval Solution (Dako, Carpinteria, CA, United States), pH 6.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5µm sections were deparaffinized with xylene and rehydrated through graded ethanol washes followed by antigen retrieval in sodium citrate buffer (10 mM, pH 6.0) with a pressure cooker (Dako, Carpinteria, CA), blocked ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence microscopy was performed on de-paraffinized tissue sections that received heat-induced antigen retrieval using Target Retrieval Solution (Agilent Dako, S169984-2). After washing and permeabilization in TBS-T universal buffer (0.2% Triton X-100 in tris-buffered saline) ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were then treated with Proteinase K (20 μg/mL) for 15 minutes for antigen retrieval and blocked with DAKO background reducing protein blocking agent (Agilent, X090930-2) prior to 1 hour incubation of CD206 (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 μm FFPE tissue sections were cut followed by an antigen retrieval by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies, Germany). Slides were incubated with a monoclonal anti-γH2A.X (phosphor-S139 ...
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bioRxiv - Neuroscience 2021Quote: ... or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005, Dako-Agilent) for 20 min at 97°C using a PT Link (Dako – Agilent) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Fixated lung tissue sections underwent antigen retrieval (pH 9 buffer) using a Dako PT Link pre-treatment module (Agilent, Santa Clara, CA). Samples were washed with PBS and blocked with Dako protein block (Agilent ...
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bioRxiv - Cancer Biology 2019Quote: ... 4-µm sections were cut and antigen retrieval was carried out by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies, Germany). The slides were stained with either polyclonal anti-SOX6 antibody raised in rabbit (1:1,600 ...
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bioRxiv - Immunology 2020Quote: ... Sections were deparaffinized by pretreatment with 0.5% H2O2 in methanol and then subjected to antigen retrieval with target retrieval solution (Dako S1700, pH 6.1) followed by heating in an autoclave for 20 min at 121 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and deparaffinized prior to antigen retrieval in 0.1M sodium citrate pH6 and staining with guinea pig anti-insulin antibody (Dako A0564, 1:250) overnight at 4 degrees in the dark and visualized with Anti-Guinea Pig biotin conjugated antibody (Vectastain BA7000 ...