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Citations for Agilent :
1 - 50 of 2337 citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
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bioRxiv - Neuroscience 2021Quote: ... (12) mouse anti-PCNA was used at 1:1000 (M0879; Dako Immunochemicals). This antibody was raised to a synthetic amino acid sequence (LVFEAPNQEK ...
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bioRxiv - Pathology 2024Quote: ... and anti- CK7 (clone OV-TL 12/30, Agilent Technologies, Santa Clara, CA, USA). Immunostaining was performed automatically using a DAKO Autostainer Link 48 machine (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... A single C4 Type A (Agilent, G9203) SPE cartridge was used for the sample clean-up ...
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bioRxiv - Bioengineering 2023Quote: ... pHelper plasmid (240071-12, Agilent Technologies) and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI ...
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bioRxiv - Immunology 2020Quote: ... Mutations of W169G on ASCCARD (Type IIa) and G20K on caspase-1CARD (Type IIb) were performed by QuikChange Mutagenesis (Agilent Technologies). All plasmids were confirmed by Sanger sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... collagen type IV (2 μg /ml clone M0785, Dako, Agilent Pathology Solutions ...
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bioRxiv - Neuroscience 2022Quote: ... wild type human TTC3 in pCMV-Tag2 vector (Stratagene), mouse AUTS2-myc-DDK in pCMV6 (OriGene ...
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bioRxiv - Biochemistry 2024Quote: ... generated from the wild-type construct using QuikChange mutagenesis (Agilent), all mutations were verified by sequencing ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The samples were loaded onto a C18 Type SPE cartridge (Agilent) using deionized water containing 0.1% trifluoracetic [TFA] at flow rate of 1.5 mL/min and eluted to the mass spectrometer using acetonitrile/deionized water (95/5 ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... PaFtsH2H1-link-12 and site-directed mutagenesis (QuickChange, Agilent) was used to create PaFtsH2H1-link-10 ...
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bioRxiv - Cell Biology 2023Quote: ... A graphite C18/type D solid phase extraction (SPE) cartridge (Agilent Technologies) was used for analyte/IS adsorption/elution ...
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bioRxiv - Biochemistry 2021Quote: ... coli K-12 strains XL1-Blue (Agilent catalog number 200236) or NEB5α (NEB catalog number C2987H ...
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bioRxiv - Microbiology 2023Quote: ... and the membrane was hybridized using QuickHyb (#201220-12 Agilent) to a 32P-labeled dsDNA NP ...
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bioRxiv - Genetics 2023Quote: ... a pHelper (240071-12, Agilent Technologies, Santa Clara, CA, USA), and an ITR-flanked AAV plasmid (pAAVsc-JeT-MCOLN1-pA ...
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bioRxiv - Microbiology 2024Quote: ... and the membrane was hybridized using QuickHyb (#201220–12 Agilent) to a 32P-labeled dsDNA NP ...
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bioRxiv - Genomics 2021Quote: ... The quality of all 12 RNA samples was checked by Agilent RNA 6000 Nano Reagents Part1 (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... using three liquid chromatography methods applied to two distinct types of mass spectrometry instruments (Agilent qTOF 6545 ...
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bioRxiv - Bioengineering 2022Quote: ... collagen type IV (2 μg /ml clone M0785, Dako, Agilent Pathology Solutions, Santa Clara, CA), fibronectin (2 μg/ml clone MAB1918 ...
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bioRxiv - Microbiology 2021Quote: ... and type 1 IFN response (Mx1) was performed with MPO (Dako Cat. No. A0398; polyclonal) at 1:1000 detection using Rabbit Polink-1 HRP ...
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bioRxiv - Physiology 2023Quote: ... Hepatocytes were then cultured in collagen type I coated XF24 cell culture microplates (Seahorse Bioscience) at 1.5 ×104 cells per well and incubated 4-6 h at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... was used with a polyethylene glycol (PEG) type polar phase column (HP-Innowax, ref. Agilent 19091N-116I ...
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bioRxiv - Neuroscience 2021Quote: ... The quality of the cDNA was assessed on a Tape 12 Station (Agilent). A total of 1 ng purified cDNA was used as starting material for the library preparation ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Mutations in the wild type heavy chain plasmid were performed using site directed mutagenesis (Agilent, 200524) following the manufacturer’s protocol to generate EG ...
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bioRxiv - Cell Biology 2024Quote: ... the 8 wild type homomers in M3925 plasmids were subjected to site-directed mutagenesis (QuikChange, Agilent). The misfolded mutants contained mutations to lysine (see Table S1 ...
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bioRxiv - Neuroscience 2021Quote: ... and cover-slipped (Catalog #12-544-E) using fluorescence mounting medium (Dako, Cat. #S3023).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... 12 ‘GG’ at rs234453358) as measured on a TapeStation (Agilent, Santa Clara, CA, USA). The cDNA libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs ...
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bioRxiv - Cell Biology 2023Quote: ... Site directed mutagenesis was performed according to manufacturer’s instructions (QuickChange II, Agilent, 200523-12). Following the PCR reaction ...
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bioRxiv - Neuroscience 2023Quote: ... The fragment length of the libraries was verified using the Fragment Analyzer 12 (Agilent). Paired-end 150- base pair reads were sequenced with the NovaSeq 6000 platform (Illumina).
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bioRxiv - Biochemistry 2020Quote: Wild-type (WT) and F94S His6-CSAD were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIPL cells (Stratagene) at +15 °C using 0.5 mM IPTG induction ...
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bioRxiv - Cell Biology 2022Quote: ... Mutations were added to wild-type H3.1-V5 and H3.3-V5 sequences using the Quikchange II XL site directed mutagenesis kit (Agilent). Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260 ...
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bioRxiv - Genetics 2020Quote: ... Mutations were introduced by site-directed mutagenesis of the wild-type gene using the QuikChange mutagenesis kit (Agilent). Sequencing was performed to confirm correct base pair substitutions or deletions were made.
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bioRxiv - Microbiology 2024Quote: Growth curves for H53 wild type and ΔcirA strains were measured using BioTek Epoch 2 microplate reader with BioTek Gen6 v.1.03.01 (Agilent). Colonies of each strain were inoculated in 5 mL Hv-Cab liquid medium and grown shaking at 45°C until an OD600 0.15 was reached ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product was cloned into pSC vector (Strataclone PCR cloning kit Agilent 240205-12) and verified by Sanger sequencing ...
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bioRxiv - Cell Biology 2020Quote: Mitochondrial respiration was examined in Chmp5Ctsk and wild-type skeletal progenitors using a Seahorse XFe96 Extracellular Flux Analyzer (Agilent) as previously described53 ...
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bioRxiv - Genetics 2022Quote: The wild-type and mutant BRD-1-BRC-1 chimeras were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). The cells were grown at 37°C until OD600 0.6 and were induced by 0.2mM isopropyl-β-D-thiogalactoside in the presence of 100μM ZnCl2 at 37°C for 6 hrs ...
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bioRxiv - Biophysics 2024Quote: Human wild-type α-synuclein and A90C cysteine variants were purified from Escherichia coli BL21 (DE3)-gold (Agilent Technologies) expressing plasmid pT7-7 encoding for α-synuclein as previously described35,42,43 ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 12-well plates were transfected with 450 ng of NF-κB-driven (Stratagene) or AP 1-driven luciferase (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... DNA concentrations were measured with NanoDrop1000 or the Advanced Analytical 12-capillary Fragment Analyzer from Agilent. Fluorescence scans of individual substances were conducted using a Tecan Infinite M1000 Pro ...
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bioRxiv - Biochemistry 2022Quote: ... The H335A mutation that renders PLC-γ1 catalytically inactive was introduced into the transfer vector encoding wild-type PLC-γ1 (residues 21-1215) or PLC-γ1 (D1165H) using standard primer-mediated mutagenesis (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: Desired mutations were introduced into mhsT wild-type gene via site-directed mutagenesis (Mutagenesis QuikChange Lightning Kit, Agilent Technolgies, Inc) using designed primers ...
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bioRxiv - Biochemistry 2021Quote: ... RpoB E310A and RpoB E310K were made by site-directed mutagenesis of the wild type constructs using the Quikchcange II XL kit (Agilent). B ...
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bioRxiv - Biochemistry 2020Quote: The FANCD2 S592A and S592D cDNAs were generated by site-directed mutagenesis of the wild type FANCD2 cDNA using the Quikchange Site-directed Mutagenesis Kit (Stratagene). The forward (FP ...
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bioRxiv - Biochemistry 2020Quote: ... The gene coding for the H349Y GlgE variant was generated from the synthesized wild-type glgE gene using a QuikChange Lightening kit (Agilent). The constructs were ligated into pET21a expression vectors (Novagen ...
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bioRxiv - Biochemistry 2020Quote: The modified pVote0GW vector used for wild-type hTop2α overexpression was mutated by site directed mutagenesis using the QuikChange XL Site-Directed Mutagenesis kit (Agilent) in order to generate the plasmids harboring K418A ...
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bioRxiv - Microbiology 2021Quote: ... D950N) were prepared from wild-type SARS-CoV-2 spike using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: ... Parkin mutant plasmids were cloned beginning with a Homo sapiens wild type eGFP-parkin vector [36] and performing PCR point mutagenesis (Quikchange Mutagenesis kit: Stratagene) utilizing primers ordered from Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: Cys to Ala substitution mutations were introduced at specific sites in the ectodomain of wild type toll by using QuickChange II XL site-directed mutagenesis kit (Stratagene) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... These genes were cloned into an Adenovirus type 5 replication-defective E1/E3 deleted vector using the Ad-Easy Adenoviral Vector System (Agilent). Briefly ...