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Citations for Agilent :
251 - 300 of 653 citations for 7 Bromo benzo c isothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: FFAs in liver tissue were extracted and measured by GC-MS (Agilent 7890 A / 5975 C) according to the method described by Tang et al(26) ...
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bioRxiv - Physiology 2019Quote: ... at 95°C for 20 min in a high pH buffer (Dako Antigen retrieval, high pH). Endogenous peroxidase was blocked using the EnVision FLEX Peroxidase-Blocking kit followed by two washes for 5 min each (Dako wash buffer) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated from 25 to 96°C in a Mx3005P real-time PCR instrument (Stratagene). Fluorescence was monitored with excitation and emission filters set to 465 and 590 nm ...
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bioRxiv - Biochemistry 2021Quote: ... proSP-C BRICHOS D105N was obtained with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, US), and the DNA sequence was confirmed (GATC Bioteq ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were incubated overnight (4 °C) with rabbit anti-GFAP (1:500; DAKO, Z0334). The antibody was prepared in 10% donkey serum in PBS containing 0.02% sodium azide and 0.3% triton X-100 ...
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bioRxiv - Immunology 2019Quote: ... for 12h at 37°C before immunostaining according to standard protocol with anti-CD3 (Polyclonal; Dako), anti-CD4 (clone 1F6 ...
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bioRxiv - Biophysics 2022Quote: ... Data acquisition and analyses were performed using OpenLAB CDS ChemStation Edition C.01.05 (Agilent Technologies, Inc.).
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bioRxiv - Microbiology 2023Quote: ... cells were grown with agitation at 37°C in an imaging multimode reader (Cytation 5, Agilent). Cell growth was monitored every 5 min by measuring absorbance at 600 nm ...
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bioRxiv - Genetics 2023Quote: ... Promoter capture Hi-C was performed using a custom-made RNA capture bait system (Agilent Technologies) consisting of 39,021 individual biotinylated RNAs targeting the ends of 22,225 promoter-containing mouse HindIII restriction fragments ...
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bioRxiv - Cell Biology 2022Quote: ... samples were exposed to antigen retrieval (10 minutes at 105°C in 1 x TRS (DAKO)) ...
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bioRxiv - Microbiology 2023Quote: ... with agitation at 37°C for 16 hours in an imaging multimode reader (Cytation 5, Agilent). Optical density at 600 nm (OD600) ...
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bioRxiv - Neuroscience 2023Quote: ... Partial purification was achieved using C-18 solid phase extraction columns (Agilent Technologies, Lake Forest, CA). A series of 4 elutions with 1.5 mL of 60% ...
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bioRxiv - Neuroscience 2023Quote: ... Mutation of Syngap1 (SYNGAP1-c.2214_2217del) was introduced by site-directed mutagenesis (QuikChange Lightning, Agilent #210518). HEK293T cells were co-transfected with expression vectors using Lipofectamin 3000 (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... at 4 °C ON and afterward incubated with rabbit anti-HA antibody 1:2000 (Dako, A0001) 2 h at RT ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatant was removed and analyzed by LC-QQQ using MRM in negative ionization mode on an EclipsePlus C-18 RRHD column (1.8 µm, 2.1 × 50 mm, r.t, Agilent) or a Poroshell 120 SB-Aq column (2.7 µm ...
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bioRxiv - Plant Biology 2020Quote: ... a 6x Histidine tag was appended to the C-terminus of rbcS via the Quikchange protocol (Stratagene). Restriction free cloning of the RBS-AtRbcLScHis cassette was utilized to insert the cassette into the multiple cloning site 1 of the pRSFDuet™-1 plasmid (Novagen) ...
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bioRxiv - Microbiology 2019Quote: ... The sub-fraction was further purified by semipreparative HPLC with C-18 column (9.4 mm × 250 mm, 5.0µm, Agilent) using an acetonitrile/water gradient (0.1% formic acid ...
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Biophysics 2020Quote: Samples were thawed and injected onto a cooled (2 °C) HPLC System (Agilent Infinity 1260, Agilent Technologies) equipped with a home packed pepsin column (IDEX guard column with an internal volume of 60 µL ...
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bioRxiv - Biophysics 2020Quote: Samples were thawed and injected onto a cooled (2 °C) HPLC System (Agilent Infinity 1260, Agilent Technologies) equipped with a home packed pepsin column (IDEX guard column with an internal volume of 60 µL ...
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bioRxiv - Neuroscience 2022Quote: ... They were then incubated overnight at +4 °C with the 6F3D anti-Aβ antibody (Dako, 1/200), polyclonal anti-tau antibody (Dako ...
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bioRxiv - Microbiology 2019Quote: Oxygen consumption rate (OCR) was measured at 37 °C using Seahorse XFp extracellular flux analyser (Seahorse Bioscience). XF cell culture microplate plates were coated with 10 μl of Cell-Tak (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... Collected volatiles were thermally desorbed for 3 min at 220°C and analyzed by GC-MS (Agilent 7820A GC interfaced with and Agilent 5977E MSD ...
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bioRxiv - Developmental Biology 2019Quote: ... was applied at 4°C overnight and secondary polyclonal goat anti-mouse-HRP (Agilent P0447; 1:1,000) was applied for 1 h at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... Root length was measured 24h upon UV-C exposure (900 J/m2) using the Stratalinker 2400 (Stratagene). The relative root growth was calculated ...
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bioRxiv - Molecular Biology 2019Quote: ... The immobilized Hi-C libraries were amplified using the SureSelectXT ILM Indexing pre-capture primers (Agilent Technologies) with eight PCR amplification cycles ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... mouse (Fig. S8b) and naked mole-rat (Fig. 2a-c) marrowSeahorse XF96 Cell Culture Microplates (Agilent Technologies) between 50-250*103 cells per 200µl of the following culture media ...
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bioRxiv - Neuroscience 2021Quote: ... The hybridized arrays were then washed and scanned using an Agilent microarray scanner (G2505 C; Agilent Technologies). Data were extracted from the scanned image using Feature Extraction version 10.5.1.1 (Agilent Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... 0-8 min with 0.1% formic acid) and a (C-18 column (4.6 × 50 mm, 1.8 μm) (Agilent). The photoproducts of DEAC-OXM were generated and analyzed in an analogous manner ...
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bioRxiv - Neuroscience 2023Quote: ... 0-8 min with 0.1% formic acid) and a C-18 column (4.6 × 50 mm, 1.8 μm) (Agilent). Each compound was assessed in triplicate ...
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bioRxiv - Microbiology 2023Quote: ... The sections were then treated for 20 minutes at 95 °C with DAKO Target Retrieval solution (Dako) for epitope retrieval ...
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bioRxiv - Biophysics 2023Quote: The samples were shortly centrifuged after thawing and injected onto a cooled (2°C) HPLC System (Agilent Infinity 1260 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C with anti-total tau primary antibody solution (1: 5000, DAKO A0024). After 1 hour incubation at RT in the corresponding secondary antibody solution (donkey anti-rabbit 800) ...
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bioRxiv - Plant Biology 2023Quote: ... volatiles were thermally desorbed for 3 min at 220 °C and analyzed using GC-MS (Agilent, USA). Helium was used as the carrier gas at a flow-rate of 1 ml min-1 with a temperature gradient of 5 °C/min from 60 °C (1 min hold ...
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bioRxiv - Neuroscience 2023Quote: ... were hydrated at 37°C for approximately 24 hours in Seahorse XF Calibrant solution (Agilent 100840-000). Cells were plated on Agilent Seahorse XF96 V3-PS Microplates (Agilent 101085-004 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... (B;C) Bioanalyzer 2100 profile and statistics using a High Sensitivity DNA Assay (Agilent Technologies, Inc 2009) of genomic DNA post sheared with Covaris g-TUBE following manufacturers protocol for 10 kb fragments (B ...
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bioRxiv - Molecular Biology 2024Quote: ... NR2F2 expression vector containing the NM_021005.4:c.737G>A (p.Arg246His) variant was generated by site-directed mutagenesis (QuikChange II, Stratagene), using the forward primer ...
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bioRxiv - Cell Biology 2022Quote: ... The Pfn1 c.318_321delTGCC mutation was introduced in the targeting vector by PCR-mediated mutagenesis (QuikChange Lightning, Agilent). Targeting of the construct was done into the E14Tg2a embryonal stem cell line ...
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bioRxiv - Genomics 2019Quote: ... The following day microarrays were scanned using an Agilent C-scanner with Feature Extraction 9.1 software (Agilent technologies). RT datasets are available in the Gene Expression Omnibus (GEO ...
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bioRxiv - Neuroscience 2019Quote: ... Incubation with the primary antibody was done overnight at 4°C and with secondary antibodies (DAKO #P0217, #P0260) for 1.5 hours at RT with washing in TBS-Tween trice for 15 minutes each after each incubation ...
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bioRxiv - Microbiology 2019Quote: ... The extract was dissolved in methanol and was subjected to preparative HPLC-MS with C-18 column (21.2 mm × 250 mm, 7.0 µm, Agilent) using an acetonitrile/water gradient (0.1% formic acid ...
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bioRxiv - Molecular Biology 2019Quote: ... The C-terminal hexahistidine tag was brought in frame by QuikChange II XL site-directed mutagenesis (Agilent #200521) using the following primers ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was analyzed on a Poroshell 120 EC-C18 column (2.7 μm, 3.0 × 50 mm, 45 °C, Agilent) using a linear gradient from 5 to 55% acetonitrile over 6.5 min with 0.1% formic acid as the aqueous mobile phase after an initial hold at 95% 0.1% formic acid for 0.5 min (0.6 mL/min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibodies at 4°C overnight: mouse monoclonal anti-CD31 (1:100, JC70A, Dako), rabbit polyclonal anti-VWF (1:100 ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaned mixtures were applied to a C-18 reverse phase column equipped on an HPLC (Agilent 1260) with a gradient of 0.1 M KH2PO4 (pH 6.0 ...
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bioRxiv - Systems Biology 2021Quote: ... for 20 min at 96°C with the reagents EnVision FLEX Target Retrieval Solution High pH (50x) (Dako) for CAT and EnVision FLEX Target Retrieval Solution Low pH (50x ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were derivatized at 70°C and injected in a splitless mode to a GC-MS system (Agilent 7890A chromatograph ...
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bioRxiv - Cell Biology 2022Quote: ... the cartridge sensors were incubated overnight at 37 °C in a hydration/ calibration solution (XF Calibrant; Agilent Technologies). Specially designed polystyrene tissue culture-treated 96-well microplates with a clear flat bottom (Seahorse XF96 V3 PS Cell Culture Microplates ...
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bioRxiv - Bioengineering 2022Quote: ... then incubated separately for 30 min at 37 °C with secondary Ab (anti-mouse ab HRP conjugated, DAKO Envision K4001 or anti-rabbit ab HRP conjugated ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...