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Citations for Agilent :
301 - 350 of 430 citations for 7 Azaspiro 3.4 octane 6 8 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cDNA samples were purified with Ampure XP beads at a 1:0.8 ratio and cDNA quality in randomly selected 10 wells/plate was assessed on a High Sensitivity Bioanalyzer chip (Agilent). cDNA concentrations were quantified using Quant-it PicoGreen dsDNA Assay kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were purified with Ampure XP beads at a 1:0.8 ratio and DNA quality in randomly selected 10 wells/plate was assessed on a High Sensitivity Bioanalyzer chip (Agilent). Libraries were quantified as mentioned above and normalized libraries were equimolarly pooled and purified one last time at a 1:0.8 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... 2000 cells were seeded on a well of a 96-well plate and brightfield images were taken every two hours by using the BioTek BioSpa 8 Automated Incubator and Citation 5 reader (Agilent). BioTek Gen5 imaging software (Agilent ...
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bioRxiv - Plant Biology 2023Quote: ... 8 and 12 h and K+ concentration in the sample was determined by ICP-OES (Agilent 710, Agilent Technologies, USA) as described previously (Cai et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Number of pre-capture PCR cycles was 8 - 14 based on input DNA quantity and quality (assessed by Agilent TapeStation) as advised by the manufacturers’ protocol and run on an AB Veriti 96-well Thermocycler (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... 8 and 12 h and K+ concentration in the sample was determined by ICP-OES (Agilent 710, Agilent Technologies, USA) as described previously (Cai et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... at a ratio of 0.8:1 beads:cDNA (20 μl beads:25 μl cDNA for Smart-seq2 and 8 μl beads:10 μl cDNA for Smart-seq3) and inspected on a Bioanalyzer 2100 (High Sensitivity DNA kit, Agilent).
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bioRxiv - Cancer Biology 2020Quote: ... Barcoded cDNA libraries containing 5-6 LMD samples plus a Universal Human Reference RNA (UHR) standard (Stratagene) were prepared on the Ion Chef System using the Ion Ampliseq Chef DL8 materials and the Ion AmpliSeq Transcriptome Human Gene Expression Panel Chef-ready Kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from near-confluent 6 cm dishes using the Absolutely RNA Miniprep kit (Agilent) including an on-column DNase treatment step according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Cultures were blocked for 45 min with blocking buffer (BB) containing 6%Goat serum (Dako, S-100) in DPBS ...
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bioRxiv - Neuroscience 2019Quote: ... PSD-95 in tissues from the L4-6 spinal cord segments were amplified by PCR (Stratagene M3005p), and a pair of GAPDH primers and Taqman probe were added into each reaction system as the internal reference of PCR amplification ...
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bioRxiv - Developmental Biology 2020Quote: ... The following primary antibodies and dilutions were used: guinea pig anti-Insulin (1:6, Dako, IR00261-2), mouse anti-Glucagon (1:500 ...
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bioRxiv - Immunology 2023Quote: The following primary antibodies were used after performing antigen retrieval with Target Retrieval solution (pH 6) (Dako): rabbit anti-DCLK1 (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... then impedance was measured every 15 min for 6 h using an xCELLigence RTCA DP instrument (Agilent). Due to the nature of the assay ...
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bioRxiv - Bioengineering 2024Quote: ... serotype 6 (AAV6) vector plasmid derived from the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA). Experiments were performed with rAAV6 vectors produced and purified by SignaGen Laboratories (Frederick ...
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bioRxiv - Molecular Biology 2019Quote: ... We measured O2 consumption of fully differentiated adipocytes at day 7 with an XF24-3 extracellular flux analyzer (Agilent Technologies, Santa Clara, CA, USA). Basal respiration was also assessed in untreated cells.
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bioRxiv - Biochemistry 2020Quote: ... linear gradient from 5% to 35% ACN in 50 mM TEAAc pH 7 over 15 min at 50 °C, Agilent Technologies Series 1200 HPLC). The collected fractions were freeze dried 3 times ...
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bioRxiv - Molecular Biology 2021Quote: ... To hybridize mRNAs or lncRNAs to whole-genome 8 × 60 K 60-mer oligonucleotide arrays (Agilent Technologies, Palo Alto, CA, USA), 200 ng of total RNA was labeled with the one-color Quick Amp labeling kit (Agilent Technologies).
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bioRxiv - Cancer Biology 2019Quote: ... Scanning of microarrays was performed with 5 μm resolution and XDR extended range (4×44K arrays) or 3 µm resolution (8×60K arrays) using a G2565CA high-resolution laser microarray scanner (Agilent Technologies). Microarray image data were analyzed and extracted with the Image Analysis/Feature Extraction software G2567AA v ...
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bioRxiv - Cancer Biology 2019Quote: Microarray experiments were performed as dual-color or single-color hybridizations on either Agilent Whole Human Genome 4×44K microarrays (Design ID 014850) or 8×60K human custom (Agilent-048908) microarrays comprising identical features for coding genes ...
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bioRxiv - Cancer Biology 2021Quote: Comparative gene expression profiling of the prostate cancer cells starved for glutamine for 24 h or cultured with glutamine supplementation was performed using SurePrint G3 Human Gene Expression 8×60K v3 Microarray Kit (Design ID 039494, Agilent Technologies) according to manufacturer’s recommendations as described previously 4 ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA from each clone was analyzed by microarray-based comparative genomic hybridization using catalog whole-genome microarrays (SurePrint G3 Human CGH microarray, 8 × 60 k format, Agilent technologies) and/or by multiplex ligation dependent probe amplification using the SHOX SALSA MLPA Probemix (assay ID ...
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bioRxiv - Neuroscience 2022Quote: ... CAS No 1582-09-8) was accessed using the Seahorse XF 96 Extracellular Flux Analyzer and the XF Cell Mito stress test kit (Agilent Technologies) following Agilent Technologies guidelines ...
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bioRxiv - Genetics 2022Quote: ... We then amplified the transposed DNA for 8 cycles and inspected for fragment length distribution using an Agilent Bioanalyzer High Sensitivity DNA Chip (Agilent Genomics). This revealed the expected nucleosomal laddering pattern ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples that passed quality control according to integrity (RIN>8) and concentration as verified on a Bioanalyzer pico chip (Agilent Technologies) were subjected to further processing by the Center for Applied Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... approximately 8 µg of protein was injected on a Zorbax 300SB-C18 column (5 µm, 300Å, 1×250mm IDxL; Agilent Technologies) and separated using a 30 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Cancer Biology 2021Quote: ... RNAs from 4 different experiments (biological quadruplicate) were extracted as described above and analyzed on SurePrint G3 Human Gene Expression 8×60K v2 Microarrays (#G4851B, Agilent Technologies) as previously described (36) ...
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bioRxiv - Cancer Biology 2021Quote: ... Scanning of microarrays was performed with 3 μm resolution (8×60K) using a G2565CA high-resolution laser microarray scanner (Agilent Technologies). Microarray image data were processed with the Image Analysis/Feature Extraction software G2567AA v ...
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bioRxiv - Bioengineering 2022Quote: Cells were dissociated from differentiation wells and seeded at 8 × 104 cells well−1 in CM support medium on Matrigel-coated XFe96 monolayer plates (Agilent Technologies). Culture medium was changed the next day to the well’s respective treatment ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended at a concentration of 8×106 cells/ml in Seahorse XF Base Medium (Agilent Technologies, Santa Clara, CA) supplemented with 1 mM pyruvate ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using a low-input QuickAmp labeling kit and human SurePrint G3 8×60K microarrays (Agilent Technologies), as previously described (Sulpice et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA integrity (all processed samples had a RIN>8) was verified with the Agilent Bioanalyzer 2100 automated electrophoresis system (Agilent Technologies). mRNA was isolated using the NEBNext® Poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8□×□60 K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Biophysics 2022Quote: ... Hela CD95KO and Hela WT stable CD95-mEGFP was assessed using the QIFIKIT® for quantification of cell surface antigens by flow cytometry (K007811-8, Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... PCR amplified libraries were purified from 8% PAGE gels and subsequently analysed with the Agilent High Sensitivity DNA assay (Agilent, Bioanalyzer).
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bioRxiv - Neuroscience 2024Quote: Free-floating brain sections (8-14 µm thick) were mounted onto slides and blocked with Protein Block (Serum-Free, Dako, CA) for 1h at RT ...
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bioRxiv - Immunology 2023Quote: ... The Cy5-labeled cDNA was hybridized overnight to 8 x 60K 60mer oligonucleotide spotted microarray slides (Agilent Technologies, design ID 074809). Hybridization and subsequent washing and drying of the slides was performed following the Agilent hybridization protocol ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL of eluted RNA and 5 µL RNA ScreenTape sample buffer was mixed in an 8-well PCR tube strip (Agilent Technologies) via vortexing for one minute (IKA MS3 Vortexer ...
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bioRxiv - Synthetic Biology 2024Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity - Waters, USA) on a C-8 column (Zorbax Eclipse XBD - Agilent, USA) at a flow rate of 0.6 mL/min and eluted off the column using a hydrophobicity gradient ...
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bioRxiv - Bioengineering 2019Quote: ... The electrical state was tested with a resistance meter (34401A 6 ½ Digit Multimeter, Agilent, Santa Clara, CA, USA) between different tapping points (connector – solder pads on interconnecting ceramic – wires anterior to interconnecting ceramic) ...
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bioRxiv - Physiology 2020Quote: ... DAPI (4′,6-diamidino-2-phenylindole; 1:1000) was used as nuclei staining and fluorescent mounting medium (DAKO) to cover the slides before imaging acquisition ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... (Mildford, MA, USA) and vacuum manifold system (VacElut 6 Manifold Processing Station, Agilent Technologies, Santa Clara, CA, USA) were used for solid-phase extraction (SPE).
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bioRxiv - Microbiology 2020Quote: ... The plasmid for production of (His)6-SUMO-σAntA-DD was generated by site-directed mutagenesis (Agilent QuikChange) using primers listed in Table S3 ...
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bioRxiv - Biochemistry 2022Quote: ... MNase-digested samples were loaded on 6% PAGE and stained with SybrGOLD and run on a Bioanalyzer (Agilent) using DNA High sensitivity chips ...
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bioRxiv - Molecular Biology 2023Quote: ... serotype 6 (AAV6) vector plasmids were cloned into the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA), comprised of inverted terminal repeats (ITRs ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were steamed for 35 minutes with a pH 6 Dako Target Retrieval (Agilent Technologies, S169984-2) and permeabilized with 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was heated to 40°C for an isocratic 6-minute run paired with a 5977B GC/MSD (Agilent).
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bioRxiv - Cell Biology 2023Quote: ... siRNA-resistant versions have been obtained by mutating 6 bp of the siRNA-targeting sequence using Quickchange (Agilent).
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bioRxiv - Genetics 2024Quote: ... serotype 6 (AAV6) vector plasmids were cloned into the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA), comprised of inverted terminal repeats (ITRs ...
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bioRxiv - Cell Biology 2020Quote: ... A 140 bp region of alpha satellite sequence from chromosome 4 and a 349 bp region of HSATII from chromosome 7 were independently cloned into pTargeT™ DNA backbone via StrataClone TA cloning (Agilent Technologies, Santa Clara, CA).