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Citations for Agilent :
151 - 200 of 518 citations for 7α 12α Dihydroxy 5β cholestan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... 3 x 150-mm Zorbax SB-Aq column (Agilent, Santa Clara, CA). A coupled DAD-3000RS diode array detector (Dionex) ...
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bioRxiv - Microbiology 2020Quote: ... with products monitored every 2 to 3 cycles on a TapeStation (Agilent) to ensure correct fragment sizes (∼500bp) ...
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bioRxiv - Cell Biology 2022Quote: ... grown cells were blocked with 1x PBS containing 3% goat serum (DAKO) for 30 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... followed by the red 3-amino-9-ethylcarbazole (AEC) HRP substrate (Dako) and counterstaining with haematoxylin ...
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bioRxiv - Neuroscience 2022Quote: ... and goat-anti-mouse IG (Dako, P0447, 1:3000 in 3% milk) antibodies were used ...
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bioRxiv - Cancer Biology 2023Quote: ... for cleaved Caspase-3 and HRP labeled polymer and DAB chromagen (Dako) for PCNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining was performed following the epitope retrieval process using VectaStain Kit from Vector Labs for cleaved Caspase-3 and horseradish peroxidase-labeled polymer from Dako (K4001) for PCNA ...
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bioRxiv - Biochemistry 2023Quote: ... The free HSF2BP protein was analyzed using a BIOSEC 3 column (Agilent) equilibrated in 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA expression was measured using SYBR green on a QuantStudio 3 (Agilent). Probes were generated using NCBI primer blast and are listed in Table X ...
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bioRxiv - Cell Biology 2020Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... One μg/ml GFP antibody (Nacali Tesque Inc., cat# GF200) and 0.5 μg/ml HRP-conjugated mouse antibody (Dako, cat# P0447) were used for detecting DNA-binding proteins ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Purified total RNA (100 ng) was labelled with Cyanine3 (Cy3) using a Low Input Quick Amp WT labelling kit (One-color; Agilent Technologies). The Cy3-labelled cRNA was checked for its amount (> 5 μg ...
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bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
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bioRxiv - Neuroscience 2019Quote: ... After one last wash with DPBS for 5 minutes at RT the coverslips were imbedded in fluorescent mounting medium (DAKO #S3023). Primary antibodies that were used ...
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bioRxiv - Physiology 2021Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies), according to the manufacturer's instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The L480P mutation in GL2 was generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) with primers listed in Supplemental Table 5 ...
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bioRxiv - Microbiology 2020Quote: ... RNA was processed and labeled for microarray analysis using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp WT Labeling kit (Agilent Technologies), according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... Double-stranded complementary DNA (cDNA) synthesis and array processing were performed by One-Color Microarray-Based gene Expression Analysis (Agilent Technologies). The images were analysed with the software DEVA (v1.2.1) ...
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bioRxiv - Microbiology 2021Quote: ... One ul of each of the RNA-seq libraries was loaded on a Bioanalyzer 2100 DNA High Sensitivity chip (Agilent Technologies) to check for size and absence of primer dimers ...
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bioRxiv - Plant Biology 2022Quote: ... then washed at room temperature using the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology, V 6.5, 2010). The hybridized array was immediately scanned with an Agilent Microarray Scanner D (Agilent Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... and gl2Amo mutants were generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies). The Phusion® Site-Directed Mutagenesis Kit (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... One final wash with PBS was performed before mounting the coverslips on glass slides using DAKO mounting medium (DAKO Cytomation, S3023) or acquiring images in PBS when using Ibidi slides.
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bioRxiv - Microbiology 2020Quote: ... Gene-level intensities were scaled based on the intensity values of ten different in vitro-synthesized transcripts contained in the One Color RNA Spike-In kit (Agilent Technologies) in order to account for technical variation associated with sample processing ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed three times with PBS and one time with distilled water and mounted with Dako Fluorescent Mounting Medium (Dako, Denmark). Samples were visualized in the epifluorescence microscope Olympus BX53 fluorescence microscopy with UPLFLN 100× oil objective (numerical aperture 1.30) ...
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bioRxiv - Genetics 2023Quote: ... Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies). The specific activities and cRNA yields were determined by using a NanoDrop ND-1000 (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... Total RNA was quantified using a NanoDropTM One spectrophotometer and quality assessed with an Agilent 2100 Bioanalyser using an RNA chip (Agilent Technologies). For library construction all RNA samples had a RIN value >7 ...
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bioRxiv - Microbiology 2021Quote: ... Subsequent antibody incubation was performed at 4°C overnight or for one hour at room temperature using Dako polyclonal goat anti-rabbit or anti-mouse immunoglobulins/HRP (Agilent Technologies, USA). Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were washed twice with PBS + 0.5% Triton X-100 and then rinsed one time with PBS before mounting with DAKO Fluorescence Mounting Medium (S3023; Dako NA Inc.). Images were analyzed with a Nikon Eclipse Ti fluorescence microscope with a Plan Fluor 40 Å∼ /1.30 Oil DIC N2 objective ...
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bioRxiv - Immunology 2020Quote: mRNA in total RNA was converted to cDNAby using AffinityScript One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA) following the method described elsewhere (17) ...
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bioRxiv - Developmental Biology 2023Quote: ... one µg of total RNA was used to synthetize cDNAs using oligo(dT) primers and affinity script reverse transcriptase (Agilent technologies France). Real-time quantitative PCR analyses were performed using the Light Cycler LC 1.5 (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... The antigen retrieval was achieved with 3 min proteinase K treatment (S3020, Agilent), and the sections were washed in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... both packed with Polaris C18-A 3-μm material (all from Agilent Corp.). After injection of the sample onto the trapping column ...
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bioRxiv - Molecular Biology 2022Quote: ... a J2-4 reverse primer 5’-(GGATAGATCTCTGAGGAGACGGTGACCAGGAG-3’) and HERC II polymerase (Agilent), following the manufacturer’s recommended reaction conditions ...
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bioRxiv - Neuroscience 2020Quote: ... a 3-minute incubation in Envision FLEX Peroxidase-blocking Reagent (GV823, Agilent DAKO) was performed ...
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bioRxiv - Neuroscience 2020Quote: ... a 3-minute incubation in Envision FLEX Peroxidase-blocking Reagent (GV823, Agilent DAKO) was performed ...
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bioRxiv - Microbiology 2022Quote: ... Agilent SEC-3 300-Å HPLC column (Agilent Technologies, cat. no. 5190-2511) was used to purify tRNA from total RNA with a temperature-controlled column compartment at 40 °C with 100 mM ammonium acetate aqueous phase at a flow rate of 1 ml/min ...
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bioRxiv - Developmental Biology 2021Quote: ... and all antibodies (Supplemental Table 3) diluted in antibody diluent solution (DAKO, S0809). Secondary staining was performed for 30 min ...
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bioRxiv - Immunology 2020Quote: ... and backbone plasmid pNL4-3.Luc.R-E-(NIH AIDS Reagent) using GeneJammer (Agilent) in 10% FBS/DMEM enriched with 1% Pennicilin/Streptomycin (D10 medium) ...
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bioRxiv - Immunology 2020Quote: ... For immunohistochemistry the antibodies detailed in follow were used: AE1/3 (Dako/IR053), TTF-1 (Dako/IR056) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... For separation a Zorbax RRHD Eclipse XDB C18 column (1.8µm, 3×50mm; Agilent) was used ...
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bioRxiv - Cell Biology 2023Quote: ... washed 3×10 minutes with PBS again and mounted using glycergel (Dako, Cat.N.C0563). The imaging was performed with the use of ZEISS microscopes ...
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bioRxiv - Neuroscience 2020Quote: ... One microliter of sample was used to determine the concentration (Qubit 3.0 fluorometer) and integrity (Agilent 2100 Bioanalyzer, Agilent High Sensitivity DNA Kit). RNA sequencing was performed using the PE100 strategy (HiSeq 2500 ...
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bioRxiv - Plant Biology 2022Quote: ... RNA labeling and hybridization were performed by using the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology, V 6.5, 2010). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... with HistoVT One (L6F9587, Nacalai Tesque) and stained with the following primary antibodies: anti-insulin antibody (A0564, 1:400; Dako, Santa Clara, CA), anti-glucagon antibody (ab92517 ...
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bioRxiv - Neuroscience 2021Quote: ... for a minimum of one month (De Guzman et al., 2016) before MRI using a 7.0 Tesla MRI scanner (Agilent Inc., Palo Alto, CA). Scanning was performed as in Misquitta et al ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... One microgram of purified cRNA was fragmented to uniform size and applied to Drosophila (V2) Gene Expression microarray (Agilent Technologies, Design ID 021791) in hybridization buffer ...
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Physiological variation reflects bioclimatic differences in the Drosophila americana species complexbioRxiv - Evolutionary Biology 2019Quote: ... Each group of 20 flies was then lightly anesthetized and weighed as a group before being irradiated with UV-B light at one of the four experimental intensities using an ultraviolet Stratalinker 2000 (Stratagene, La Jolla, CA). For the 0J exposure - which essentially measures longevity in the absence of acute UV exposure - flies were simply anesthetized ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then transferred into a buffer storage solution of 0.1M PBS with 2mM ProHance and 0.02% sodium azide for a minimum of one month (De Guzman et al., 2016) using a 7.0 Tesla MRI scanner (Agilent Inc., Palo Alto, CA) as in Anacker et al ...