Labshake search
Citations for Agilent :
551 - 600 of 4557 citations for 6 chloro 2 N 2 N diethyl 4 N propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: Reagents for immunohistochemistry were HRP-conjugated anti-rabbit Ig (Agilent Cat# K400311-2), HRP-conjugated anti-mouse Ig (Agilent Cat# K400111-2) ...
-
bioRxiv - Neuroscience 2024Quote: ... Coverslips were secured onto slides with fluorescence mounting medium (Dako Agilent, #S302380-2) and imaged using a Zeiss Inverted Microscope attached to a Dell PC ...
-
bioRxiv - Neuroscience 2024Quote: ... Coverslips were secured onto slides with fluorescence mounting medium (Dako Agilent, #S302380-2) and imaged using a Zeiss Inverted Microscope attached to a Dell PC ...
-
bioRxiv - Cancer Biology 2024Quote: ... or 2 mM L-glutamine for the Glycolysis Stress Test (Agilent #103020-100). After an hour incubation at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... 2 cm x 100 µm) before being separated on an analytical column (Agilent Poroshell EC-C18 ...
-
bioRxiv - Cancer Biology 2022Quote: ... the slices were incubated with primary antibodies: rabbit polyclonal anti-PSMA/GCPII (1:150) (M362029-2; DAKO, US), anti-CD68 (1:250 ...
-
bioRxiv - Genomics 2020Quote: ... 1 - 2 ng / µl was examined using a 2100 Bioanalyzer and a corresponding High Sensitivity DNA Kit (Agilent) to determine the MW profile of the size-selected library ...
-
bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 15 min) before incubation with either rabbit anti-mouse IgG (1.3 µg ml−1, 1% BSA in PBST; Agilent, P026002-2), goat anti-rabbit IgG (250 ng ml−1 ...
-
bioRxiv - Cell Biology 2020Quote: ... and a mix of rotenone/antimycin A (stock solution 50 μM) (cat n. 103015-100, Agilent, Santa Clara, USA). Crystal violet was purchased from Sigma-Aldrich (cat n ...
-
bioRxiv - Bioengineering 2020Quote: ... The PHA monomers’ methylesters were assayed by GC using a Hewlett-Packard 6890 N chromatograph equipped with a HP-Innowax capillary column (30 m × 0.25 mm, 0.50-μm film thickness; Agilent Technologies) and a flame ionization detector (FID) ...
-
bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... N≥50 worms (10 worms/ well) were transferred to the Seahorse XF96 Cell Culture Microplate (Agilent Technologies, 101085-004) in a total volume of 180 uL ...
-
bioRxiv - Immunology 2022Quote: ... The final set of nucleotide sequences was collapsed at 100% nucleotide sequence identity (n = 238,068) and then ordered from Agilent Technologies.
-
bioRxiv - Bioengineering 2023Quote: ... After calibrating and verifying the performance of the column using the LC Bio-standard (Agilent, P/N : 5190-9417), the recombinant antibody samples were diluted to 2mg/ml in 1XPBS and loaded onto a pre-conditioned column at a flow rate of 0.35ml/min ...
-
bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
-
bioRxiv - Cell Biology 2021Quote: ... Single cells were separated using a 100 μm strainer and labelled with CD31 antibody (1:200, M082329-2, DAKO) at 4°C for 45 min followed by 30 min staining with a goat anti-mouse secondary antibody conjugated to AlexaFluor 488 or 555 (1:400 ...
-
bioRxiv - Immunology 2022Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Basal OCR and ECAR were measured in the presence of Oligomycin (1.5 μM ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2% BSA (PAA Laboratories GmbH, Germany) and 1:50 dilution of normal goat serum (Dako Denmark A/S, Denmark). Subsequently ...
-
bioRxiv - Immunology 2020Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
-
bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
-
bioRxiv - Biophysics 2021Quote: ... 2 mL of the cell suspension was loaded into a quartz cuvette (Agilent Technologies). Fluorescence was measured with an Agilent Cary Eclipse fluorescence spectrophotometer with slit widths at 5 and 10 nm for excitation wavelength of 370 nm and emission wavelength of 415 nm ...
-
bioRxiv - Developmental Biology 2022Quote: ... 2 µg amplified cDNA was Cy5-labeled using the SureTag DNA labeling kit (Agilent). Hybridization to 8×60K 60mer oligonucleotide spotted microarray slides (Human Mouse Genome ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... Data were analysed with the 2–ΔCt method on MxPro qPCR software (Agilent Technologies), and values are expressed as the mean of duplicates.
-
bioRxiv - Pathology 2019Quote: ... Slides were then washed in 1X EnVision FLEX Wash Buffer (Agilent DAKO, K800721-2) for 5 minutes ...
-
bioRxiv - Pathology 2019Quote: ... Slides were then washed in 1X EnVision FLEX Wash Buffer (Agilent DAKO, K800721-2) for 5 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... unfrationated 2-5A oligomers were run on an HPLC (1260 Infinity II Agilent technologies) equipped with a preparative Dionex column (BioLCRDNAPacRPA-100 ...
-
bioRxiv - Cancer Biology 2020Quote: ... and -2 of the zymogen sequence of KLK3 (Quick Change Lightning Mutagenesis Kit; Stratagene) enabled furin ...
-
bioRxiv - Cancer Biology 2022Quote: ... Stainings were visualized using Liquid DAB+ 2-component system (3,3’-diaminobenzidine, DAKO, Agilent K3467) following the manufacturer’s protocol and washed three times with TBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... Stainings were visualized using Liquid DAB+ 2-component system (3,3’-diaminobenzidine, DAKO, Agilent K3467) following the manufacturer’s protocol and washed three times with TBS ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then washed 2 times with XF DMEM assay medium (Agilent, #103575-100), supplemented with glucose (Agilent ...
-
bioRxiv - Cell Biology 2020Quote: ... RT-qPCR assays were analyzed with 2(-ΔΔCt) method 85 via MxPro software (Stratagene) and expressed as relative quantity to normalizer 86.
-
bioRxiv - Neuroscience 2022Quote: ... the slides were exposed to a mouse-specific biotinylated secondary antibody (GV82111-2, Dako) for 15 minutes ...
-
bioRxiv - Immunology 2020Quote: ... 500 ng of F(ab’)2 fragments of α-μ (clone JDC-15; Dako [α-human] ...
-
bioRxiv - Microbiology 2019Quote: ... The cells were stained with HAstV mouse monoclonal antibody 8E7 (2 μg/ml DakoCytomation) for 1 hour at room temperature followed by anti-mouse IgG labeled with Alexa Fluor 488 (anti-mouse IgG-Alexa Fluor 488 ...
-
bioRxiv - Genomics 2020Quote: ... and two Peltier thermal stations (CPAC Ultraflat HT 2-TEC, #7000166A, Agilent Technologies, USA) with PCR adapter having a mounting frame at positions 4 and 6 on the Bravo Deck and connected to an Inheco MTC Controller ...
-
bioRxiv - Immunology 2021Quote: ... washed twice with PBS/2% FCS and stained with PE (Prozyme, 20 µg/ml). RNA flow cytometry measurement of Bhlhe40 expression was performed using the PrimeFlow RNA Assay (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... and 2) size distribution using the High Sensitivity DNA BioAnalyzer kit (Agilent, 5067-4626). Libraries were constructed using the Nextera XT library Prep kit (Illumina ...
-
bioRxiv - Cell Biology 2023Quote: ... and 2 µM Antimycin A + Rotenone (Seahorse XF Cell Mito Stress Test Kit, Agilent) was added sequentially via injection ports ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by HRP-conjugated anti-mouse secondary antibody polymer (EnVision+; Dako-Cat# K400311-2) and visualized by Cy7-tyramide as substrate ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM glutamine and 10 mM glucose (All from Agilent, Santa Clara, CA, USA). The plates were read using a XF HS Mini analyser (Agilent ...
-
bioRxiv - Cell Biology 2024Quote: ... and incubated with primary antibodies diluted in background reducing antibody diluent (Agilent S302283-2) overnight at RT ...
-
bioRxiv - Immunology 2024Quote: ... and 2 mM glutamine and analyzed with an XF-96 Extracellular Flux Analyzer (Agilent). Four consecutive measurements were obtained under basal conditions followed by the addition of 1 μM oligomycin ...
-
bioRxiv - Microbiology 2024Quote: ... for 24 h or by 15 µg/ml α-human IgG (Agilent #A042301-2) for 48 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... β1-4 galactosidase (Agilent Technologies), or double digestion with Sialidase A and β-galactosidase ...
-
bioRxiv - Immunology 2024Quote: ... β(1-4)-Galactosidase (Prozyme) (designated as “G”) ...
-
bioRxiv - Biophysics 2022Quote: ... 1 and 3.5 μm diameter fibers were manufactured from 2 and 5 wt% of high molecular weight polystyrene (MW: 15,000,000 g/mol, Agilent Technologies, Santa Clara, CA, USA) and equally spaced at approximately 60 μm ...
-
bioRxiv - Plant Biology 2023Quote: ... Ten-μl B314-2 sample was injected into an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 μm, Agilent, Santa Clara, CA, USA) and eluted with the same gradient solvent system described above ...
-
Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Coverslips were then incubated with 1X DAPI for 5 min before mounting on slides using DAKO mounting media (Agilent Technologies, Cat #S302380-2). Images were captured at 60X or 100X using a Nikon C2+ confocal microscope coupled to NIS-Elements AR software (Nikon).
-
bioRxiv - Molecular Biology 2023Quote: ... Desorption was performed at 250°C for 1’ (splitless mode) in the injection port of a 6890 N gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) coupled to PEGASUS 4D mass spectrometer (LECO Corporation) ...