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Citations for Agilent :
151 - 200 of 531 citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... One hundred microliters of the supernatant were transferred to an autosampler vial and analyzed by gas chromatography-mass spectrometry (Agilent 8890 Gas Chromatograph and Agilent 7000D Mass spectrometer) ...
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bioRxiv - Systems Biology 2023Quote: ... and digested using a modified in-solution sample preparation protocol.94 One hundred micrograms of desalted peptides were fractioned on an microflow HPLC (1260, Agilent) using a XBridge peptide BEH C18 column (130 Å ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was amplified and Cy3-labeled by using the one-color Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The E50A variant was further obtained by site-directed mutagenesis using an adapted protocol from the Stratagene “QuickChange” with only one mutator primer carrying the modified sequence and with both the Pfu turbo polymerase (Stratagene) and the Taq ligase (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... 90 µl of ATP reagent was added to each well and ATP-dependent bioluminescence (RLU1) was measured after one minute as relative light units (RLU) by Synergy H1 plate reader (BioTek, Agilent). Two wells without cells were used as a blank (RLU0) ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were microwaved in pH 6 antigen retrieval solution (1X Target Retrieval Solution, Agilent Cat # S1699) to bring near to ...
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bioRxiv - Genomics 2020Quote: ... Adapter-ligated DNA was amplified for 6-8 cycles using Herc II Fusion DNA polymerase (Agilent). PCR products were purified with AMPure XP beads and subjected to Illumina paired-end sequencing (2x 75 bp).
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bioRxiv - Immunology 2023Quote: ... Heat-induced epitope retrieval was performed for 15 min in Target Retrieval solution (pH 6) (Dako) and all other steps were as described above for mouse snout processing ...
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bioRxiv - Immunology 2024Quote: ... Antigen retrieval was performed using Antigen Retrieval Solution (pH 6 or pH 9) (Dako, Glostrup, Denmark) for at least 15 minutes in an autoclave ...
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bioRxiv - Biochemistry 2022Quote: ... with 4 cycles per step using Seahorse XFe96 Analyzer (Agilent). Cell Mito Stress Test was used for assessing mitochondrial function ...
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bioRxiv - Biophysics 2019Quote: ... A 4 mm triple-resonance HXY MAS NMR probe (Agilent) was used under static condition ...
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bioRxiv - Cell Biology 2020Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... One μg/ml GFP antibody (Nacali Tesque Inc., cat# GF200) and 0.5 μg/ml HRP-conjugated mouse antibody (Dako, cat# P0447) were used for detecting DNA-binding proteins ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Purified total RNA (100 ng) was labelled with Cyanine3 (Cy3) using a Low Input Quick Amp WT labelling kit (One-color; Agilent Technologies). The Cy3-labelled cRNA was checked for its amount (> 5 μg ...
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bioRxiv - Neuroscience 2019Quote: ... After one last wash with DPBS for 5 minutes at RT the coverslips were imbedded in fluorescent mounting medium (DAKO #S3023). Primary antibodies that were used ...
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bioRxiv - Physiology 2021Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies), according to the manufacturer's instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The L480P mutation in GL2 was generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) with primers listed in Supplemental Table 5 ...
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bioRxiv - Microbiology 2020Quote: ... RNA was processed and labeled for microarray analysis using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp WT Labeling kit (Agilent Technologies), according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... Double-stranded complementary DNA (cDNA) synthesis and array processing were performed by One-Color Microarray-Based gene Expression Analysis (Agilent Technologies). The images were analysed with the software DEVA (v1.2.1) ...
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bioRxiv - Microbiology 2021Quote: ... One ul of each of the RNA-seq libraries was loaded on a Bioanalyzer 2100 DNA High Sensitivity chip (Agilent Technologies) to check for size and absence of primer dimers ...
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bioRxiv - Plant Biology 2022Quote: ... then washed at room temperature using the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology, V 6.5, 2010). The hybridized array was immediately scanned with an Agilent Microarray Scanner D (Agilent Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... and gl2Amo mutants were generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies). The Phusion® Site-Directed Mutagenesis Kit (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... One final wash with PBS was performed before mounting the coverslips on glass slides using DAKO mounting medium (DAKO Cytomation, S3023) or acquiring images in PBS when using Ibidi slides.
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bioRxiv - Microbiology 2020Quote: ... Gene-level intensities were scaled based on the intensity values of ten different in vitro-synthesized transcripts contained in the One Color RNA Spike-In kit (Agilent Technologies) in order to account for technical variation associated with sample processing ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed three times with PBS and one time with distilled water and mounted with Dako Fluorescent Mounting Medium (Dako, Denmark). Samples were visualized in the epifluorescence microscope Olympus BX53 fluorescence microscopy with UPLFLN 100× oil objective (numerical aperture 1.30) ...
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bioRxiv - Genetics 2023Quote: ... Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies). The specific activities and cRNA yields were determined by using a NanoDrop ND-1000 (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... Total RNA was quantified using a NanoDropTM One spectrophotometer and quality assessed with an Agilent 2100 Bioanalyser using an RNA chip (Agilent Technologies). For library construction all RNA samples had a RIN value >7 ...
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bioRxiv - Cancer Biology 2020Quote: ... Barcoded cDNA libraries containing 5-6 LMD samples plus a Universal Human Reference RNA (UHR) standard (Stratagene) were prepared on the Ion Chef System using the Ion Ampliseq Chef DL8 materials and the Ion AmpliSeq Transcriptome Human Gene Expression Panel Chef-ready Kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from near-confluent 6 cm dishes using the Absolutely RNA Miniprep kit (Agilent) including an on-column DNase treatment step according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Cultures were blocked for 45 min with blocking buffer (BB) containing 6%Goat serum (Dako, S-100) in DPBS ...
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bioRxiv - Neuroscience 2019Quote: ... PSD-95 in tissues from the L4-6 spinal cord segments were amplified by PCR (Stratagene M3005p), and a pair of GAPDH primers and Taqman probe were added into each reaction system as the internal reference of PCR amplification ...
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bioRxiv - Developmental Biology 2020Quote: ... The following primary antibodies and dilutions were used: guinea pig anti-Insulin (1:6, Dako, IR00261-2), mouse anti-Glucagon (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... then impedance was measured every 15 min for 6 h using an xCELLigence RTCA DP instrument (Agilent). Due to the nature of the assay ...
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bioRxiv - Immunology 2023Quote: The following primary antibodies were used after performing antigen retrieval with Target Retrieval solution (pH 6) (Dako): rabbit anti-DCLK1 (Abcam ...
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bioRxiv - Bioengineering 2024Quote: ... serotype 6 (AAV6) vector plasmid derived from the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA). Experiments were performed with rAAV6 vectors produced and purified by SignaGen Laboratories (Frederick ...
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bioRxiv - Microbiology 2021Quote: ... Subsequent antibody incubation was performed at 4°C overnight or for one hour at room temperature using Dako polyclonal goat anti-rabbit or anti-mouse immunoglobulins/HRP (Agilent Technologies, USA). Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were washed twice with PBS + 0.5% Triton X-100 and then rinsed one time with PBS before mounting with DAKO Fluorescence Mounting Medium (S3023; Dako NA Inc.). Images were analyzed with a Nikon Eclipse Ti fluorescence microscope with a Plan Fluor 40 Å∼ /1.30 Oil DIC N2 objective ...
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bioRxiv - Immunology 2020Quote: mRNA in total RNA was converted to cDNAby using AffinityScript One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA) following the method described elsewhere (17) ...
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bioRxiv - Developmental Biology 2023Quote: ... one µg of total RNA was used to synthetize cDNAs using oligo(dT) primers and affinity script reverse transcriptase (Agilent technologies France). Real-time quantitative PCR analyses were performed using the Light Cycler LC 1.5 (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 µm tissue sections were stained and developed using AutostainerPlus (Dako). Antibodies were diluted in block solution and sections were incubated for 30 minutes with primary antibody and 20 minutes with secondary antibodies ...
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bioRxiv - Synthetic Biology 2020Quote: ... or Streptococcus pneumoniae β-N-Acetylhexosaminidase (Prozyme, 4 mU per digest) overnight at 37°C in 20 mM sodium acetate (pH 5.0).
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bioRxiv - Cancer Biology 2019Quote: ... 4°C hold) by Herculase II fusion enzyme (Agilent Technologies, #600679). After each step DNA was purified by Ampure XP beads (Beckmann Coulter Genomics #A63882) ...
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bioRxiv - Cancer Biology 2019Quote: Whole Human Genome Microarray Kit 4×44K (Agilent, Cat. No. G4112F) was used to detect mRNA expression levels in cells transfected with control and miR-101-3p transfected HCT116 cells ...
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bioRxiv - Cell Biology 2021Quote: ... overnight at 4°C after blocking with blocking solution (S3022, Dako) for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Each SBC mouse (4*180K) LncRNA microarray slide (Agilent Technologies Inc.) was hybridized with 1.65 μg Cy3-labeled cRNA using a gene expression hybridization kit (Agilent Technologies ...
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bioRxiv - Immunology 2019Quote: ... and then overnight at 4°C in rabbit anti-CD3 (Dako) in 1% (v/v ...
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bioRxiv - Genetics 2020Quote: ... at 4°C followed by secondary antibody (Dako Cytomation, Glostrup, Denmark) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... using the Canis (V2) Gene Expression Microarray 4×44K (Agilent Technologies). Microarray data were subjected to gene ontology (GO ...