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Citations for Agilent :
501 - 525 of 525 citations for 6 Chloro 2H chromene 3 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Primary antibodies were diluted 1:4000 for anti-sGCα1 and 1:2000 for anti-sGCβ1 antibody in 3% dry milk in TBST and incubated with nitrocellulose membranes at 4°C over-night following challenge of membranes with secondary goat anti-rabbit antibody (1:2000 in 3% milk in TBST) conjugated to horseradish peroxidase (Dako A/S, Denmark). Immuno-complexes were visualized using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech ...
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bioRxiv - Biochemistry 2022Quote: 50 μL of pure CrRPE1 concentrated at 9.5 mg mL−1 were injected on BioSEC-3 300 size-exclusion chromatography column (Agilent Technologies, Santa Clara, USA) equilibrated in buffer 20 mM Tris-HCl (pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- ACC TCC ACC ACC AGA ACC ACC TCC ACC GCT CTG AAA GTA CAG ATC CTC GGT ACC TTT ATA TAA TTC GTC CAT-3’) using the Agilent QuikChange SDM kit following the manufacturer’s instructions (Agilent, Singapore, Singapore; Cat: STR_210519). This was followed by amplifying the backbone of the plasmid using primer set Ag43BBGib_F ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in micebioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared using an automated - QuantSeq 3’mRNA-seq (Lexogen GmbH, Vienna) and Agilent NGS Workstation (Agilent Technologies, Santa Clara) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Biophysics 2021Quote: ... and endogenous peroxidase was blocked with 3% H2O2 in TBS for 5 min followed by incubation with anti-mouse EnVision+ labelled polymer (Agilent Technologies, Santa Clara, USA) for 30 min ...
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bioRxiv - Immunology 2022Quote: Naive CD3+ T cells were placed on PLL-coated Seahorse Bioanalyzer XFp culture plates (3 × 105 cells/well) with Seahorse XF RPMI Assay Media (RPMI Medium, pH 7.4, 103576-100; Agilent Technologies, Santa Clara, CA, USA), supplemented with 10 mM glucose (103577-100 ...
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bioRxiv - Cell Biology 2022Quote: ... were diluted with ultra-pure water to reduce the acid concentration below 3% and loaded into the ICP-MS-MS (triple quad Agilent 8800x ICP-MS-MS). The instrumental conditions were optimized to remove interferences by using a collision/reaction cell ...
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bioRxiv - Molecular Biology 2019Quote: ... We measured O2 consumption of fully differentiated adipocytes at day 7 with an XF24-3 extracellular flux analyzer (Agilent Technologies, Santa Clara, CA, USA). Basal respiration was also assessed in untreated cells.
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Physiology 2021Quote: ... was amplified by PCR using cDNA of mouse kidney at the template with the primers 5′-CCGTCGACATGCAGGTCAAGAAATCCTG-3′ (SalI site in italics) and 5′-CCGGATCCTTAAAGGCGTGTGTGATCTT-3′ (M6 reverse primer; BamHI site in italics) and cloned into pBluescript SK(−) (Stratagene, La Jolla, CA, USA) using the SalI and BamHI sites ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For phthalates and BPA extraction we used Agilent Vac Elut Manifold with SPE cartridge C18 ODS 3 ml tubes 200 mg (Agilent Santa Clara CA, USA). SPE cartridges were conditioned with 2 ml of methanol and then 2 ml of MilliQ water ...
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bioRxiv - Molecular Biology 2022Quote: ... amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8×60K V2 Arrays (SurePrint G3 Human Gene Expression 8×60K v2 Microarray Kit ...
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bioRxiv - Synthetic Biology 2023Quote: Samples (3 µL) were injected into an ion mobility drift tube-quadrupole time-of-flight mass spectrometer (LC-IM-QTOF, Agilent 1290 series LC 6560). An Agilent Poroshell 120 HILIC-Z 150 × 2.1 mm ...
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bioRxiv - Neuroscience 2023Quote: ... 50,000 cells per well were plated after 3 days of induction in a specialized Seahorse analyzer 96-well plate (Agilent, cat. no. 103774-100). Cells were washed in PBS once and incubated in a hypoxic chamber following the manufacturer’s procedure ...
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bioRxiv - Neuroscience 2022Quote: ... samples were suspended in a 4M urea buffer containing ampholytes and subjected to off-gel IEF fractionation using a 24 well strip (pH 3-10) (Agilent, 3100 Off- gel fractionator). Following IEF ...
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bioRxiv - Neuroscience 2023Quote: ... 50,000 cells per well were plated after 3 days of induction in a specialized Seahorse analyzer 96-well plate (Agilent, cat. no. 103774-100). 10-12 day old i3Neurons were treated with 5 nM vincristine ...
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bioRxiv - Cancer Biology 2019Quote: ... For 8 other samples (1 normal and 3 tumor regions each from 2 patients) only DNA was isolated and targeted panel sequencing (Agilent SureSelect run on a HiSeq) was performed for 257 genes ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF7-ESR1Y537S cells were seeded at a density of 3×104 in corresponding treatment media without phenol red in each well of the XFp Cell Culture miniplates (Seahorse Bioscience Inc., Billerica, MA, USA). The next day ...
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were incubated for at least 20 min at 25°C before 10 μl were injected onto a SEC-3 HPLC column (300 Å pore size; Agilent Technologies, Santa Clara, CA) equilibrated with SEC buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 3C library preparation and target enrichment using a custom-designed collection of 6979 biotinylated RNA “baits” targeting single MboI restriction fragments chr15:10283500-13195800 (mm9) (Suppl. Table S2; Agilent Technologies; as in Ref.3) were performed following the SureSelectXT Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library protocol ...
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bioRxiv - Pathology 2023Quote: ... Sections from the first level (main experiment) were stained for smooth muscle alpha-2 actin (ACTA2) and galectin 3 (LGALS3) using mouse monoclonal anti-ACTA2 (Dako, cat. no. M0851, 1:100) after blocking with Fab fragment (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2021Quote: ... Collected volatiles were thermally desorbed for 3 min at 220°C and analyzed by GC-MS (Agilent 7820A GC interfaced with and Agilent 5977E MSD, Palo Alto, CA, USA). Desorbed volatiles were separated with split-less mode on a column (HP5-MS ...