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Citations for Agilent :
301 - 350 of 350 citations for 6 Aminomethylnicotinic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... the RNA integrity number was 10 as assessed by the Stanford Protein and Nucleic Acid (PAN) Biotechnology Facility using the RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-SΔ19 expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) by following the manufacturer’s instructions and using mutagenic oligonucleotides SaCoV2-K417T-F ...
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bioRxiv - Cancer Biology 2022Quote: ... RAS double mutants with point-mutations in the 95th and 96th amino acid residues were generated by site-directed mutagenesis (Agilent Technologies #200521) using the primers listed in Supplementary Table 2 per manufacturer’s recommended PCR conditions ...
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bioRxiv - Physiology 2023Quote: ... High-resolution gas chromatography capillary column coated with 0.25µm film thickness was used (DB-FFAP) for the detection of volatile acids (Agilent Technologies, Santa Clara, CA). The oven temperature was 145°C and the FID and injection port were set to 225°C ...
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bioRxiv - Microbiology 2023Quote: ... Elemental quantification on acid-digested liquid samples was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA). The following settings were fixed for the analysis Cell Entrance = −40 V ...
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bioRxiv - Neuroscience 2021Quote: ... For ChR2-mediated optogenetic stimulation, blue light (473 nm, 6 Hz or 20 Hz) was produced using an arbitrary waveform generator (Agilent, 33220A) and a diode-pumped solid-state laser (Laserglow ...
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bioRxiv - Developmental Biology 2019Quote: ... The quality of the purified DNA library was assessed on 6%TBE gels as well as on a Bioanalyzer (2100 Expert software, Agilent Technologies) using High Sensitivity DNA Chips (5067-4626 ...
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bioRxiv - Developmental Biology 2020Quote: ... RIN scores (RIN = 10 for all 6 samples) were measured on a BioAnalyzer 2100 using the RNA 6000 Nano assay (Agilent Technologies). Poly(A)-purified mRNA-seq library preparation was performed according to the TruSeq Stranded mRNA Reference Guide (Illumina) ...
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bioRxiv - Synthetic Biology 2021Quote: Thermal denaturation experiments were performed using a qCHANGER 6/Cary60 (Quantum Northwest) interfaced to a Cary 60 UV-Visible spectrophotometer (Agilent Technologies) using a custom ADL script developed by Quantum Northwest to collect full spectra at each temperature as described previously ...
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bioRxiv - Developmental Biology 2021Quote: ... for 6 minutes (30 seconds ON, 30 seconds OFF) at 4°C to obtain ~200 bp fragments (confirmed using an Agilent Bioanalyzer). Sonicated DNA was extracted using ethanol precipitation ...
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bioRxiv - Molecular Biology 2023Quote: The complex formation between CtBP1 and CtBP2 variants and RAI2 variants was investigated on an analytical Superose 6 3.2/300 GL column (Cytiva, MA, USA) coupled to a 1260 Infinity HPLC system (Agilent, CA, USA). Proteins were dialyzed against the SEC buffer and injected onto a pre-equilibrated S6 column using an autosampler ...
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bioRxiv - Cancer Biology 2024Quote: ... using miR30-adapted sequences (5-6 shRNAs per target) by PCR-cloning a pool of oligonucleotides synthesized on 55k customized arrays (Agilent Technologies) using a well-established system 84–87 ...
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bioRxiv - Molecular Biology 2019Quote: ... Amino acids were analyzed using an Agilent 1260 Infinity High Performance Liquid Chromatography System equipped with ChemStation (Agilent Technologies, Santa Clara, CA, USA) by injecting 5 µL of the derivatization mix onto an AccQTag™ Amino Acid Analysis C18 (Waters ...
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bioRxiv - Genomics 2019Quote: ... Nucleic acid concentrations were measured by NanoDrop ND-1000 spectrophotometer and RNA integrity was analyzed using Agilent 2100 Bioanalyzer (Agilent Technologies cat.# G2939BA).
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bioRxiv - Biochemistry 2019Quote: ... Amino acids were then analyzed by liquid chromatography coupled to a TOF MS (1290 UHPLC 6530 QTOF; Agilent Technologies, Santa Clara CA, USA) according to Gu et al ...
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bioRxiv - Pathology 2020Quote: ... resuspended to 0.5 µg/µL in 90% ACN/0.1% trifluoroacetic acid (TFA) and fractionated with an Agilent 1200 high performance liquid chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA) on an in-house 17 cm TSKgel Amide-80 HILIC column (3 µm particle size ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-Strunc expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) using mutagenic oligonucleotides as follow:
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bioRxiv - Immunology 2022Quote: FFPE tissue sections were deparaffinized and antigen retrieval was undertaken in 0.1 M citric acid buffer solution (Dako, target retrieval solution, S169984-2) using an electric pressure cooker (Russell Hobbs RHNHP401) ...
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bioRxiv - Physiology 2021Quote: ... Amino acid substitution mutants were generated by site-directed mutagenesis using a QuickChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA, USA). The predicted DNA sequences of all constructs were verified by sequencing.
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Bioengineering 2023Quote: ... An aliquot of 1 mL was obtained and diluted with 2% nitric acid before running for elemental analysis using an inductively coupled plasma optical emission spectrometer (ICP-OES, Varian 720-ES, Agilent, Santa Clara, CA). For each run ...
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... We extracted extracellular metabolites using solid phase extraction (SPE) with 1 g/6 cc PPL cartridges (BondElut, Agilent, Santa Clara, CA, USA) as described previously (Dittmar et al ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the extraction of amanitins was performed using a vacuum manifold system (VacElut 6 Manifold Processing Station, Agilent Technologies, Santa Clara, CA, USA) with a 6 positions rack and OASIS® PRIME HLB cartridges (1 mL/30 mg ...
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bioRxiv - Pathology 2020Quote: ... Deparaffinization and antigen retrieval were performed in Target Retrieval Solution with pH 9 (ACE2) or pH 6 (Ki-67) at 97°C for 20 min using the PT Link platform (Dako, Glostrup, Denmark). Subsequently ...
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bioRxiv - Synthetic Biology 2021Quote: 384 well qPCR reaction plates for the multiplexed activation of endogenous genes (Figure 5 and 6) were set up using the Brilliant II SYBR master mix (Agilent cat #600828) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... in addition to forward scattering and side scattering pulse area and width measurements (FSC-A, FSC-H, SSC- A, and SSC-H): cytoplasmic TdT/FITC (clone HT-6, Agilent/Dako, cat. F7139), CD56/PE (clone C5.9 ...
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bioRxiv - Cancer Biology 2023Quote: ... depending on the antibody used (HIF-1α, Dako Target Retrieval Solution pH 6; for BACH-1, Dako Target Retrieval Solution pH 9). Next ...
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bioRxiv - Bioengineering 2023Quote: ... bEnd.3 cells were seeded at a density of 6×103 cells/well in rat tail collagen-coated (150 µg/mL) 96-well plates (E-plate 96; Agilent, cat# 300600910) and confluent monolayers were treated as described above for human ECs ...
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bioRxiv - Bioengineering 2023Quote: ... rat ECs were seeded at a density of 6×103 cells/well in 96-well plates (E-plate 96; Agilent, cat# 300600910) coated with collagen IV (100 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... and branched-chain amino acids (BCAAs) was performed with an Agilent 7890A gas chromatograph coupled with an Agilent 5975 mass spectrometer (Agilent Technologies Santa Clara, CA) using a propyl esterification method as previously described [61] ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were loaded with solvent A (0.1% formic acid in water) onto an in-house packed analytical column filled with Poroshell EC120 C18 material (Agilent Technologies, Santa Clara, CA, USA). Peptides were chromatographically separated at a constant flow rate of 250 nL/min using the following gradient ...
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bioRxiv - Cell Biology 2022Quote: ... were diluted with ultra-pure water to reduce the acid concentration below 3% and loaded into the ICP-MS-MS (triple quad Agilent 8800x ICP-MS-MS). The instrumental conditions were optimized to remove interferences by using a collision/reaction cell ...
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bioRxiv - Biochemistry 2022Quote: ... system coupled to an Agilent 6470 series QQQ mass spectrometer (MS/MS) was used to analyze fatty acids in each sample (Agilent Technologies, Santa Clara, CA). The methods are similar to what is described in the paper by Yang ...
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bioRxiv - Physiology 2024Quote: ... Etomoxir (an inhibitor of long chain fatty acid oxidation) provided in the Seahorse XF Mito fuel flex test kit (Agilent Technologies, Cat#103260-100). Fuel dependency for a specific pathway was measured after inhibiting this pathway ...
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bioRxiv - Genetics 2021Quote: ... and followed by endogenous HRP quenching before heat-induced epitope retrieval in citrate buffer pH 6 (Target Retrieval Solution, DAKO, cat. number S1700). After epitope retrieval ...
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bioRxiv - Cancer Biology 2022Quote: ... in addition to forward scattering and side scattering pulse area and width measurements (FSC-A, FSC-H, SSC- A, and SSC-H): cytoplasmic TdT/FITC (clone HT-6, Agilent/Dako, cat. F7139), CD56/PE (clone C5.9 ...
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bioRxiv - Plant Biology 2022Quote: ... About 1 μg of total RNA per tissue type at a quality of RNA integrity number ≥ 6 based on a Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) were used to generate RNA-Seq libraries ...
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bioRxiv - Neuroscience 2024Quote: ... 10ug of total synaptosomal protein was plated in each well of a polyethyleneimine coated 96 well plate (n=5-6/animal) and analyzed using the MitoStress kit from Agilent (Santa Clara, CA). The MitoStress kit measures oxygen consumption rate (OCR ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were heated with sodium citrate (pH=6) for antigen retrieval and blocked with a Protein Block reagent (Dako, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... The collection of 152 LMNA substitution mutants and 6 multispecies variants assayed in this study were generated by QuikChange™ mutagenesis (Stratagene, San Diego, CA) with degenerate oligonucleotides or ...
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bioRxiv - Bioengineering 2021Quote: ... The serial dilution was prepared by the robotic liquid handler in a 6 × 8 (48-well square) deep well plate (Agilent Part number 201306-100) according to the DilutionPlan (Section 3.1.3) ...
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bioRxiv - Bioengineering 2021Quote: ... and MBP-tagged (for immunoblotting) NC1 domains of type IV Collagen α1-6 (V) were expressed in BL21-CodonPlus-RP (Agilent Technologies, Santa Clara, CA) transformed with pET-28a (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Cell nuclei were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 5 min before being mounted using DAKO mounting medium (Agilent, catalog no. S302380–2). Imaging of the histological samples was performed on the Microscope Axio Imager.A2 (Carl Zeiss Microscopy ...
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bioRxiv - Microbiology 2020Quote: ... was studied in 50 ml resting cell as mentioned above were monitored till 12 h in an interval of 2 h and later till 48h in an interval of 6 h for determining the rate of CP degradation using high performance liquid chromatography (HPLC) (Agilent Technology 1260 Infinity, USA).
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bioRxiv - Biochemistry 2021Quote: ... Antigen retrieval using a standard pH 6 sodium citrate buffer (BioGenex) was performed and sections were stained with Monoclonal Mouse Anti-Human CD45 (Dako, M0701, dilution 1:200) using the M.O.M ...
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bioRxiv - Cell Biology 2022Quote: ... 2cm x 100µM) for 5min in solvent A (0.1% formic acid in water) before being separated on an analytical column (Agilent Poroshell, EC-C18, 2.7µM, 50cm x 75µM). Solvent B consisted of 0.1% formic acid in 80% acetonitrile ...
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bioRxiv - Pathology 2019Quote: Paraffin-embedded tissue sections were processed for antigen retrieval by heat-induced epitope retrieval in citrate buffer pH 6 (DAKO, Glostrup, Denmark, cat. number S2367). After epitope retrieval ...