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Citations for Agilent :
351 - 400 of 690 citations for 15 PGDH HPGD Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Whole-exome libraries were prepared using a SureSelectXT Human All Exon V5 kit (Agilent Technologies). The RNA seq library from tumor RNA was prepared using Illumina TruSeq Stranded mRNA Library Prep Kit as per the manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: OCR in human liver cells was measured using XFp Extracellular Flux Analyzers (Agilent Seahorse Biosciences). The cells were plated into XFp cell culture mini plates for 24 h ...
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bioRxiv - Developmental Biology 2021Quote: Libraries were generated using the Agilent SureSelect Human All Exon V6 kit (Agilent Technologies, USA) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... cDNA for Sp110 CARD (aa 6-110) were amplified from MegaMan Human Transcriptome Library (Stratagene). cDNA for the tandem SUMO interaction motifs of RNF4 (aa 38-129 ...
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bioRxiv - Immunology 2019Quote: ... and used as hybridization probe on Whole Human Genome Microarrays (Agilent Technologies, Palo Alto, CA). Only probes with signal values >60% quantile in at least one condition were considered for the differential gene expression (DGE ...
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bioRxiv - Neuroscience 2021Quote: ... CD20 (monoclonal mouse – anti-human CD20 IgG2a, clone L26, cat. no. M075501-2, Agilent Technologies), 1:800 ...
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bioRxiv - Immunology 2021Quote: ... beads were incubated with 5 μg/ml FITC-conjugated rabbit anti-human C1q (Dako, F0254).
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bioRxiv - Cancer Biology 2021Quote: ... and enriched with the human kinome DNA capture baits (Agilent Technologies, Santa Clara, CA, USA). Six libraries were pooled for each capture reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 ng of genomic DNA from the test samples and human reference DNA (Agilent Technologies) were differentially labeled with Cy5-dCTP and Cy3-dCTP by random priming ...
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bioRxiv - Cancer Biology 2023Quote: ... and the SureSelect XT HS Human All Exon V7 target-enrichment probes (5191-4028, Agilent) for exome capture ...
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bioRxiv - Biochemistry 2023Quote: ... genomic DNA libraries were prepared using the SureSelect Human All Exon V6 kit (Agilent Technologies). Exome libraries were subjected to next generation sequencing using DNBseq platform (MGI ...
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bioRxiv - Genetics 2023Quote: ... exome enrichment was performed using the SureSelect Human All Exon 50 Mb Kit V5 (Agilent). Sequencing was done on a HiSeq4000 (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... Immunodetection of TTR monomers was performed by employing rabbit anti-human TTR polyclonal antibody (Dako) as a primary and goat anti-rabbit antibody labelled with DyLight 680 (SERACARE ...
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bioRxiv - Immunology 2024Quote: ... WES libraries were prepared using the SureSelect Human All Exon V8 Kit (Agilent; 5191-6873) and sequenced on either a NextSeq 2000 (Illumina ...
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bioRxiv - Biophysics 2021Quote: ... The samples were heated up with a ramp rate of 1 °C/min over a temperature range of 15-95 °C using the qPCR System MX 3005 P (Stratagene). Measurements were performed in triplicate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... with 15 PCR cycles used for the final amplification step and passed through quality control using a 2100 Bioanalyzer (Agilent). Both quality control and sequencing was carried out at Babraham Institute Next Generation Sequencing facility ...
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bioRxiv - Zoology 2020Quote: ... Determination of the fatty acids were run with an Agilent 7890 series gas chromatographer equipped with a 15 m × 0.25 mm DB-WAX column (Agilent, USA), with helium as the carrier gas at 1.4 mL/min ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids expressing gfpA206K fusions to mgrB mutants (pSY3-9, pSY72-75, pJC1-7, pTG1-15) were created by either QuikChange site-directed mutagenesis (Stratagene) or Inverse PCR [58] using pAL38 as template ...
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bioRxiv - Neuroscience 2020Quote: ... the DAKO Autostainer Plus was used for a blocking step during 15 min at room temperature with a commercial wash buffer from DAKO supplemented with 3% normal goat serum ...
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bioRxiv - Plant Biology 2022Quote: ... The digested solution was diluted to 15 mL with ultrapure water and analyzed by ICP-OES (Vista-PRO CCD Simultaneous ICP-OES; Agilent) calibrated with standards ...
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bioRxiv - Microbiology 2023Quote: ... The isolation of RNA fragments corresponding to ribosomal footprints of 15 – 45 nt size was verified using the RNA 6000 Nano Kit (Agilent) and the Agilent 2100 Bioanalyzer ...
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bioRxiv - Genomics 2023Quote: ... The RNA quality was validated using NanoDrop and Agilent DNF-472 HS RNA (15 nt) Kit (Agilent Technologies, CA, USA) on the 5200 Agilent system to confirm the purity ...
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bioRxiv - Plant Biology 2023Quote: ... and portions of samples corresponding to 5 µg of peptide were analyzed by nanoflow reverse-phase liquid chromatography-mass spectrometry using a 15 cm C18 Zorbax column (Agilent), a Dionex Ultimate 3000 RSLC nano-UPLC system ...
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bioRxiv - Genomics 2023Quote: ... OD600 measurements were performed at 30°C every 15 min until a plateau was reached in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent).
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence emission at 340 nm from the reaction mixture was monitored at 37°C with 15 s intervals using a Biotek Synergy H1 microplate reader equipped with Biotek Gen5 software (Agilent). A linear fit was performed for the measured fluorescence within 10 min reaction time to obtain the rate of decrease in NADH fluorescence ...
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bioRxiv - Microbiology 2024Quote: ... with aeration and optical density (600 nm) was measured in 10-15 min intervals using either a LogPhase 600 (Agilent) or BioScreen C (Growth Curves USA ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed with PBS for 15 mins (3×5min fresh solution) and cover-slipped with fluorescent mounting medium (Dako).
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bioRxiv - Microbiology 2023Quote: ... 5 ml of supernatant was volumetrically subsampled into 15 ml centrifuge tubes for colorimetric spectroscopy using the Mo-Blue method (Cary 8454 UV-vis spectrometer, Agilent) as mentioned earlier for the analysis of inorganic P.
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bioRxiv - Immunology 2023Quote: ... antigen retrieval using sodium citrate buffer (10 mM, pH 6) was performed for 15 min at 110 °C in a DAKO Pascal pressure chamber (Agilent). After blocking in PBS supplemented with 10% goat serum (Sigma Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... then was injected to online SEC-SAXS equipped with a temperature-controlled (15 °C) silica-based SEC column (Agilent BioSEC-3)32 ...
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bioRxiv - Microbiology 2021Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate kept at 250 μl/ml consisting of a 2 minutes hold at 10% B ...
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bioRxiv - Cancer Biology 2019Quote: ... for 15 min and subsequently replaced with anti-249T-P pAbs diluted at 1:250 with antibody diluent solution (Agilent Technologies). Slides were incubated at 4°C overnight and then washed and visualized with DAB+ substrate chromogen (Agilent Technologies) ...
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bioRxiv - Plant Biology 2019Quote: ... 60 °C for 20 s and 72 °C for 15 s (Clontech SYBR Green Master Mix and Mx3000P qPCR Systems, Agilent Technologies). Primers for circadian clock genes were designed using pcrEfficiency (Mallona et al. ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval was performed for 15 min in the autoclave (250°F) using 1x Target antigen retrieval solution (Dako; S169984-2). All antibody steps were performed as described above ...
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bioRxiv - Microbiology 2022Quote: ... The ligation product was enriched with 15 PCR cycles and the final library was validated on an Agilent 2100 Bioanalyzer (Agilent Technologies) using the DNA 7500 kit (Agilent Technologies).
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bioRxiv - Microbiology 2022Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate was kept at 250 μL mL−1 consisting of a 2 min hold at 10% B ...
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bioRxiv - Microbiology 2022Quote: ... containing 1 µM sulfadimethoxine as an internal standard before analyzing 15 µL on a 1290 Infinity II UHPLC system (Agilent Technologies) coupled to an ImpactII ultra-high resolution Qq-TOF mass spectrometer (Bruker Daltonics ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in blocking solution (DAKO, Mississauga, Ontario, Canada). Sections were incubated overnight in polyclonal rabbit anti-GFAP primary antibody and monoclonal mouse anti-tau antibody (DC190) ...
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bioRxiv - Microbiology 2022Quote: ... The sections were sensitized using EnVision FLEX for 15 minutes and visualized by peroxidase-based enzymatic reaction with 3,3’-diaminobenzidine tetrahydrochloride (Dako, Cat# DM827) as substrate for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The sections were sensitized using EnVision FLEX for 15 minutes and visualized by peroxidase-based enzymatic reaction with 3,3’-diaminobenzidine tetrahydrochloride (Dako, Cat# DM827) as substrate for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Sample pH was then adjusted to 4 with 15 μL of 20% formic acid before loading onto conditioned SPE Bond-Elute pH columns (Agilent, 14102062) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were incubated for 15 min with an anti-mouse secondary antibody conjugated to a dextran-labeled polymer and HRP (DakoCytomation K4007), followed by incubation in DAB solution (DakoCytomation DAB # K4007 ...
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bioRxiv - Microbiology 2023Quote: ... The sections were sensitized using EnVision FLEX for 15 minutes and visualized by peroxidase-based enzymatic reaction with 3,3′-diaminobenzidine tetrahydrochloride (Dako, Cat# DM827) as substrate for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 min) before incubation with either rabbit anti-mouse IgG (1.3 µg ml−1, 1% BSA in PBST; Agilent, P026002-2), goat anti-rabbit IgG (250 ng ml−1 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in a blocking solution (DAKO, Ontario, Canada). Sections were incubated overnight in a polyclonal rabbit anti-IBA-1 primary antibody (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The sections were sensitized using EnVision FLEX for 15 m and visualized by peroxidase-based enzymatic reaction with 3,3′-diaminobenzidine tetrahydrochloride (Dako, Cat# DM827) as substrate for 5 m ...
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bioRxiv - Physiology 2024Quote: ... were subjected to quantitative PCR under a temperature profile of 95°C for 3 min followed by 40 cycles for 95°C for 5 sec and 58°C for 15 sec using the Stratagene Mx3000P (Agilent Technologies). For each of the samples ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 5 x 15 min in PBST at RT and slides were mounted in DAKO fluorescent mounting medium (Agilent Technologies). EdU staining was performed on sections from larvae that received an EdU pulse ...
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bioRxiv - Bioengineering 2024Quote: ... IHC antibodies: rabbit anti-EGFR (clone SP84, Cell Marque #14R-15) at 1:25 and mouse anti-Ki67 (clone MIB1, Dako #M7240) at 1:50 with ME Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... libraries for whole-exome sequencing were prepared using the SureSelect Human All Exon V7 kit (Agilent) and the Illumina TruSeq Exome kit ...