Labshake search
Citations for Agilent :
4901 - 4950 of 8383 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... was used as a template for cDNA synthesis with the Affinity Script QPCR cDNA Synthesis Kit (Agilent Technologies). RT-qPCR and data analysis were performed as described in [75] ...
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bioRxiv - Microbiology 2021Quote: The NdeI site within the TbFUT1 sequence was eliminated using the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) and primers P24/P25 ...
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bioRxiv - Microbiology 2020Quote: ... and rRNA removal efficiency was then analyzed via the Agilent 2100 Bioanalyzer RNA 6000 Pico kit (Agilent Technologies). Library preparation for sequencing on the Illumina HiSeq platform was completed using the TruSeq stranded mRNA HT library preparation kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... RNA quality was measured using the Agilent 2100 Bioanalyzer RNA 6000 Nano kit (Agilent Technologies, Santa Clara, CA) and all RNA samples utilized for downstream RNAseq library preparation had an RNA integrity number (RIN ...
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bioRxiv - Microbiology 2020Quote: ... Mutations were introduced using standard PCR or using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent, 210515). Double A3H fusion constructs I-I ...
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bioRxiv - Microbiology 2020Quote: ... R134A and E321N) were incorporated into the gene sequence using the QuickChange XL site-directed mutagenesis kit (Stratagene), along with a set of several primers ...
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bioRxiv - Cancer Biology 2020Quote: ... Sample integrity was evaluated using the Agilent Bioanalyzer RNA Nano and Pico kits (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: ... we performed the mitochondrial stress test using the Seahorse Mito-stress test kit (part nb 103015-100, Agilent) with a final concentration of 4mM Oligomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Site-directed mutagenesis with a Stratagene QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent, Inc. Santa Clara, CA) was used to generate pom152Y180L,Q182L-GFP (pKBB553 ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was amplified and labeled with the Low Input Quick-Amp Labeling Kit (Agilent Technologies, # 5190-2305). In brief ...
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bioRxiv - Genetics 2022Quote: ... and its quality was assessed using a 2100 Bioanalyzer Expert system using a total RNA nano Kit (Agilent), all samples displayed an RNA integrity number (RIN ...
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bioRxiv - Microbiology 2022Quote: ... IN mutations were introduced in the pLR2P-VprIN plasmid using the QuikChange Site- Directed Mutagenesis kit (Agilent Technologies). The presence of the desired mutations and the absence of unwanted secondary changes was assessed by Sanger sequencing.
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bioRxiv - Immunology 2022Quote: ... MARS-seq barcoded RT primers were used for reverse transcription with the Affinity Script cDNA Synthesis Kit (Agilent) in a 10 μl reaction volume.
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bioRxiv - Neuroscience 2022Quote: ... All RNA samples were validated for high quality using Bioanalyzer RNA 6000 Pico Kit (Agilent, San Diego, CA). 1 ng of total RNA was used to generate full length cDNA using Clontech’s SMART-Seq v4 Ultra Low Input RNA Kit ...
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bioRxiv - Genomics 2022Quote: ... cDNA and library quality were determined using the High Sensitivity DNA Kit on a Bioanalyzer 2100 (Agilent Technologies). Multiplexed RNA-seq libraries were sequenced as paired end 150 runs on a NextSeq 500 to a sequencing depth of ~5 million read pairs.
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Metabolic switching and cell wall remodelling of Mycobacterium tuberculosis during bone tuberculosisbioRxiv - Microbiology 2022Quote: ... tuberculosis RNA was accomplished using One-Color Microarray-Based Low Input Quick Amp WT Labelling kit (Agilent Technologies) as per the manufacturer’s protocol using 300ng of input RNA ...
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bioRxiv - Cell Biology 2022Quote: Emerin mutants were generated using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies; Santa Clara, CA) according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA quality was assessed on a bioanalyzer using the Agilent RNA 6000 Nano kit (5067-1511; Agilent Technologies); 0.2-1 μg of RNA with RNA integrity number > 7 proceeded for library preparation using an Illumina TruSeq RNA prep kit ...
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bioRxiv - Immunology 2022Quote: ... L452R and E484Q for the κ variant) were introduced using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... and samples were run on a fragment analyzer by using the High Sensitivity NGS Fragment Analysis Kit (Agilent) to define the size of cfDNA extracted and genomic DNA contamination ...
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bioRxiv - Cell Biology 2022Quote: ... the stop codon was first mutagenized to glycine using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) with the STOP-to-glycine primers ...
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bioRxiv - Developmental Biology 2022Quote: ... size profiles examined in the Bioanalyzer using the DNA High Sensitivity DNA Kit (Agilent, Part No. 5067-4626). Samples were pooled and sequenced in a NovaSeq by Novogene (Cambridge ...
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bioRxiv - Cancer Biology 2022Quote: ... the quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Mutagenesis was performed using the QuikChange Lightning Multi site directed mutagenesis kit (Agilent, Santa Clara, USA, Cat#210513) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Removal efficiency of rRNA was confirmed using an Agilent 2100 Bioanalyzer system and RNA 6000 Nano kit (Agilent). Enriched mRNA samples were then used for RNA sequencing using an Illumina NextSeq ...
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bioRxiv - Microbiology 2021Quote: ... for correct size and purity with the NGS standard sensitivity fragment analysis kit (Agilent Cat. No. DF-473). Of all suitable samples a pool of 2 nM was generated and loaded to the Illumina MySeq 2500 system.
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bioRxiv - Developmental Biology 2020Quote: ... product size distribution and quantity were assessed on a Bioanalyzer using a High Sensitivity DNA Kit (Agilent Technologies). A total of 140 pg of the amplified cDNA was fragmented using Nextera XT (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... IN mutations were introduced into the pLR2P-vprIN plasmid using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies). Presence of the desired mutations and absence of unwanted secondary changes were verified by Sanger sequencing.
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with freshly prepared AB mix of Vector Elite ABC Kits and with DAB (DAKO Agilent Pathology Solutions ...
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bioRxiv - Immunology 2021Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and Bioanalyzer Agilent 2100 using a High Sensitivity DNA chip (Agilent). Indexed libraries were pooled according to number of cells and sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... Size and quantity was determined using the Bioanalyzer High Sensitivity DNA Analysis Agilent High Sensitivity DNA Kit (Agilent). Samples were sequenced according to the Illumina user manual with 80 cycles of read 1 (forward ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-ALG-2 was obtained by performing a reverse mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) on a GFP-hALG-2 Y180A (a generous gift from Masatoshi Maki ...
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bioRxiv - Neuroscience 2020Quote: ... The quality and quantity of RNA was assessed using the Agilent RNA 6000 Nano Kit Bioanalyzer (Agilent Technologies). All samples had RIN values between 9 and 10 ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody incubation lasted 2h and was followed by avidin-biotin complexes for signal amplification (ABC kit, Dako). Immunoreactivities were visualized using 3,3’-diaminobenzidine as a chromogen ...
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bioRxiv - Neuroscience 2021Quote: ... The library sizes and quantification were determined using the Bioanalyzer High Sensitivity DNA kit (Cat# 5067-4626, Agilent). The libraries were sequenced using the HiSeq2000 (Illumina).
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bioRxiv - Neuroscience 2021Quote: ... The library sizes and quantification were determined using the Bioanalyzer High Sensitivity DNA kit (Cat# 5067-4626, Agilent). The libraries were sequenced using the Nextseq500 (Illumina ...
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bioRxiv - Biochemistry 2020Quote: ... R256L and Y250L in AQP3 mutations were inserted using the QuikChange II kit (Agilent, Santa Clara, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Mutant libraries of CI2 WT and CI2 I57A were generated using the GeneMorph II Random mutagenesis kit (Agilent). The mutation frequency was aimed at 0-4 amino acid substitutions per gene by adjusting the initial target DNA and the number of amplification cycles ...
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bioRxiv - Neuroscience 2022Quote: ... Phospho-null mutation VASP S239A was generated using the QuikChange II Site-Directed Mutagenesis Kit (Cat.#200523, Agilent). Primers were designed using the QuikChange Primer Design Program and obtained from Integrated DNA Technologies IDT ...
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bioRxiv - Developmental Biology 2022Quote: ... Quality and yield of isolated RNA was assessed using the Eukaryotic Total RNA 6000 Nano kit (Agilent technologies) and the Queensland Brain Institute’s Bioanalyser 2100 (Agilent technologies) ...
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bioRxiv - Microbiology 2022Quote: ... Site-directed mutagenesis of these plasmids for C483A or Pol(-) mutants were obtained with the QuikChange kit (Stratagene) using primers listed in Supplementary Table 1 ...
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bioRxiv - Biochemistry 2022Quote: ... L452R and T478K for the δ variant) were introduced using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and isolated RNA was quantified and its quality was assessed using the RNA 6000 Pico Bioanalyzer kit (Agilent). PBMC RNA samples had RNA integrity number (RIN ...
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bioRxiv - Evolutionary Biology 2022Quote: Random mutagenesis of the hdp1 and hdp2 genes was performed with the GeneMorph II Random Mutagenesis Kit (Agilent) using the recommended conditions for obtaining a mean mutation rate of 1 mutation per PCR product ...
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bioRxiv - Immunology 2022Quote: ... Knockout PCR products were cloned into pSC-B-amp/kan with the StrataClone Blunt PCR Cloning Kit (Agilent) and Sanger sequenced to determine the exact deletion ...
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bioRxiv - Cell Biology 2022Quote: ... Mutations were added to wild-type H3.1-V5 and H3.3-V5 sequences using the Quikchange II XL site directed mutagenesis kit (Agilent). Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260 ...
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bioRxiv - Zoology 2019Quote: ... boddarti Elovl5 sequence was carried out using the QuikChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA) according to the manufacturer’s protocol with primers listed in Table 1 using the pGEM®-T Easy vector containing the B ...
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bioRxiv - Microbiology 2019Quote: ... Insert sizes and concentrations for each library were determined with an Agilent Bioanalyzer DNA 100 kit (Agilent Technologies). Libraries were finally sequenced on the Illumina NextSeq platform at the MIT Biomicro Center to generate 2 × 150 bp paired reads ...
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bioRxiv - Genomics 2019Quote: ... Small and long RNA quality was assessed using an Agilent 2100 Bioanalyzer and RNA 6000 nano kits (Agilent) and quantified on a Nanodrop spectrophotometer.
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bioRxiv - Developmental Biology 2019Quote: ... RNA quality was evaluated using Agilent Bioanalyzer with RNA 6000 Pico kit (Agilent Technologies Japan, Ltd., Tokyo, Japan). An amount of 500 ng total RNA was used for RNA-seq library preparation with NEB NEBNext rRNA Depletion Kit and ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs Japan Inc. ...