Labshake search
Citations for Agilent :
4801 - 4850 of 7040 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... L452R and E484Q for the κ variant) were introduced using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... HIV-1 GagG2A -EGFP was made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). Venus6 was a gift from Steven Vogel (76 ...
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bioRxiv - Genomics 2022Quote: ... and samples were run on a fragment analyzer by using the High Sensitivity NGS Fragment Analysis Kit (Agilent) to define the size of cfDNA extracted and genomic DNA contamination ...
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bioRxiv - Cell Biology 2022Quote: ... the stop codon was first mutagenized to glycine using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) with the STOP-to-glycine primers ...
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bioRxiv - Developmental Biology 2022Quote: ... size profiles examined in the Bioanalyzer using the DNA High Sensitivity DNA Kit (Agilent, Part No. 5067-4626). Samples were pooled and sequenced in a NovaSeq by Novogene (Cambridge ...
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bioRxiv - Cancer Biology 2022Quote: ... the quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Mutagenesis was performed using the QuikChange Lightning Multi site directed mutagenesis kit (Agilent, Santa Clara, USA, Cat#210513) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Removal efficiency of rRNA was confirmed using an Agilent 2100 Bioanalyzer system and RNA 6000 Nano kit (Agilent). Enriched mRNA samples were then used for RNA sequencing using an Illumina NextSeq ...
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bioRxiv - Microbiology 2021Quote: ... for correct size and purity with the NGS standard sensitivity fragment analysis kit (Agilent Cat. No. DF-473). Of all suitable samples a pool of 2 nM was generated and loaded to the Illumina MySeq 2500 system.
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bioRxiv - Developmental Biology 2020Quote: ... product size distribution and quantity were assessed on a Bioanalyzer using a High Sensitivity DNA Kit (Agilent Technologies). A total of 140 pg of the amplified cDNA was fragmented using Nextera XT (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... IN mutations were introduced into the pLR2P-vprIN plasmid using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies). Presence of the desired mutations and absence of unwanted secondary changes were verified by Sanger sequencing.
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bioRxiv - Genomics 2021Quote: ... Exome capture was performed using a SureSelect Human All Exon V6 capture kit (Agilent Technologies, Santa Clara, CA). Samples were sequenced on a HiSeq 4000 next-generation sequencing system (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with freshly prepared AB mix of Vector Elite ABC Kits and with DAB (DAKO Agilent Pathology Solutions ...
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bioRxiv - Immunology 2021Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and Bioanalyzer Agilent 2100 using a High Sensitivity DNA chip (Agilent). Indexed libraries were pooled according to number of cells and sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... Size and quantity was determined using the Bioanalyzer High Sensitivity DNA Analysis Agilent High Sensitivity DNA Kit (Agilent). Samples were sequenced according to the Illumina user manual with 80 cycles of read 1 (forward ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-ALG-2 was obtained by performing a reverse mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) on a GFP-hALG-2 Y180A (a generous gift from Masatoshi Maki ...
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bioRxiv - Neuroscience 2020Quote: ... The quality and quantity of RNA was assessed using the Agilent RNA 6000 Nano Kit Bioanalyzer (Agilent Technologies). All samples had RIN values between 9 and 10 ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody incubation lasted 2h and was followed by avidin-biotin complexes for signal amplification (ABC kit, Dako). Immunoreactivities were visualized using 3,3’-diaminobenzidine as a chromogen ...
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bioRxiv - Neuroscience 2021Quote: ... The library sizes and quantification were determined using the Bioanalyzer High Sensitivity DNA kit (Cat# 5067-4626, Agilent). The libraries were sequenced using the HiSeq2000 (Illumina).
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bioRxiv - Neuroscience 2021Quote: ... The library sizes and quantification were determined using the Bioanalyzer High Sensitivity DNA kit (Cat# 5067-4626, Agilent). The libraries were sequenced using the Nextseq500 (Illumina ...
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bioRxiv - Biochemistry 2020Quote: ... R256L and Y250L in AQP3 mutations were inserted using the QuikChange II kit (Agilent, Santa Clara, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Mutant libraries of CI2 WT and CI2 I57A were generated using the GeneMorph II Random mutagenesis kit (Agilent). The mutation frequency was aimed at 0-4 amino acid substitutions per gene by adjusting the initial target DNA and the number of amplification cycles ...
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bioRxiv - Neuroscience 2022Quote: ... Phospho-null mutation VASP S239A was generated using the QuikChange II Site-Directed Mutagenesis Kit (Cat.#200523, Agilent). Primers were designed using the QuikChange Primer Design Program and obtained from Integrated DNA Technologies IDT ...
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bioRxiv - Developmental Biology 2022Quote: ... Quality and yield of isolated RNA was assessed using the Eukaryotic Total RNA 6000 Nano kit (Agilent technologies) and the Queensland Brain Institute’s Bioanalyser 2100 (Agilent technologies) ...
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bioRxiv - Microbiology 2022Quote: ... Site-directed mutagenesis of these plasmids for C483A or Pol(-) mutants were obtained with the QuikChange kit (Stratagene) using primers listed in Supplementary Table 1 ...
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bioRxiv - Biochemistry 2022Quote: ... L452R and T478K for the δ variant) were introduced using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and isolated RNA was quantified and its quality was assessed using the RNA 6000 Pico Bioanalyzer kit (Agilent). PBMC RNA samples had RNA integrity number (RIN ...
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bioRxiv - Evolutionary Biology 2022Quote: Random mutagenesis of the hdp1 and hdp2 genes was performed with the GeneMorph II Random Mutagenesis Kit (Agilent) using the recommended conditions for obtaining a mean mutation rate of 1 mutation per PCR product ...
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bioRxiv - Cell Biology 2022Quote: ... Mutations were added to wild-type H3.1-V5 and H3.3-V5 sequences using the Quikchange II XL site directed mutagenesis kit (Agilent). Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260 ...
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bioRxiv - Zoology 2019Quote: ... boddarti Elovl5 sequence was carried out using the QuikChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA) according to the manufacturer’s protocol with primers listed in Table 1 using the pGEM®-T Easy vector containing the B ...
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bioRxiv - Microbiology 2019Quote: ... Insert sizes and concentrations for each library were determined with an Agilent Bioanalyzer DNA 100 kit (Agilent Technologies). Libraries were finally sequenced on the Illumina NextSeq platform at the MIT Biomicro Center to generate 2 × 150 bp paired reads ...
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bioRxiv - Genomics 2019Quote: ... Small and long RNA quality was assessed using an Agilent 2100 Bioanalyzer and RNA 6000 nano kits (Agilent) and quantified on a Nanodrop spectrophotometer.
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bioRxiv - Developmental Biology 2019Quote: ... RNA quality was evaluated using Agilent Bioanalyzer with RNA 6000 Pico kit (Agilent Technologies Japan, Ltd., Tokyo, Japan). An amount of 500 ng total RNA was used for RNA-seq library preparation with NEB NEBNext rRNA Depletion Kit and ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs Japan Inc. ...
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bioRxiv - Neuroscience 2019Quote: Target enrichment by liquid-phase hybridization capture was performed using the SureSelect Mouse Methyl-Seq kit (Agilent Technologies) 42 ...
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bioRxiv - Pathology 2019Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). Library construction was done using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Microbiology 2019Quote: ... A site-directed mutant of Pnf (toxoid) was generated with either the QuikChange site-directed mutagenesis kit (Agilent). To construct the Pnf mutant plasmid pET15b-PnfC190A ...
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bioRxiv - Immunology 2019Quote: ... Baseline ECAR and changes in glycolytic rate were assessed using the Seahorse XFp Glycolytic Rate Assay Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... RNA-seq libraries for control bulk RNA were generated using the SureSelect Strand-specific kit (Agilent Technologies, G9691B). Bulk DNA from over 1 × 105 cells was obtained from the cell culture (T0.5 sample ...
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bioRxiv - Plant Biology 2019Quote: ... Site-directed mutagenesis was carried out using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent, CA, USA) according to manufacturer's instructions.
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bioRxiv - Molecular Biology 2019Quote: ... The average size and concentration of libraries were determined using the 2100 Bioanalyzer High Sensitivity DNA Kit (Agilent) and qPCR with SensiMix SYBR (Bioline ...
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bioRxiv - Cell Biology 2019Quote: ... the pMTT1-GFP-LF4A plasmid was subjected to site-directed mutagenesis using the QuikChange Lightning kit (Agilent 210518) with the primers 5’-CAGGACGTTTGGCACTAGTGGCTGAATTGATGGATCAGAACC-3’ and 5’-GGTTCTGATCCATCAATTCAGCCACTAGTGCCAAACGTCCTG-3’.
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bioRxiv - Biochemistry 2019Quote: ... coli GyrB overexpression was mutated by site directed mutagenesis using the QuikChange XL Site-Directed Mutagenesis kit (Agilent) in order to generate two plasmids harboring R286K or R286Q mutations ...
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bioRxiv - Molecular Biology 2020Quote: ... Preparations were analyzed using an Agilent 2100 Bioanalyzer and Agilent Small RNA kit (Agilent Technologies, Santa Clara CA).
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bioRxiv - Microbiology 2020Quote: ... pT7/EV-A71BrCr and pCMV/hRV14 by using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). All mutants were verified by sequencing after mutagenesis ...
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bioRxiv - Physiology 2021Quote: ... RNA integrity was verified using an Agilent Technologies 2100 Bioanalyzer with the RNA 6000 Nano Kit (Agilent Technologies). RNA quantity was determined using a Nanodrop ND-1000 spectrophotometer (PEQlab Biotechnologie GmbH) ...
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bioRxiv - Molecular Biology 2020Quote: ... L765A) of this fragment were cloned from this vector using a QuikChange II site-directed mutagenesis kit (Agilent). The DNA construct used for proteins for crystallography was similar except it coded for PARP-1 662-1011 (again with V762A ...
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bioRxiv - Neuroscience 2020Quote: ... PLCγ2-myc-DDK was subjected to site-directed mutagenesis (QuikChange Lightning Multi Site-Directed Mutagenesis Kit, Agilent, 210515) to create PLCγ2-P522R-myc-DDK (P522R ...
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bioRxiv - Plant Biology 2019Quote: ... which was Cy3 labelled via the Low Input Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, CA, USA). The labelling efficiency ...
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bioRxiv - Molecular Biology 2021Quote: ... The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent) (Figure S5) ...
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bioRxiv - Microbiology 2021Quote: ... and DNF-473 Standard Sensitivity NGS Fragment Analysis Kit (1 bp −6000 bp; Agilent, Santa Clara, CA, USA). Average library length was 341 bp ...