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Citations for Agilent :
4801 - 4850 of 7361 citations for Chick ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... RNA quality and quantity were evaluated using Bioanalyzer 2100 with the RNA 6000 Nano Kit (Agilent Technologies). The preparation of RNA library and transcriptome sequencing was performed using an external vendor (Novogene ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting libraries were examined for size and quality using the Bioanalyzer High Sensitivity DNA Kit (Agilent) and their concentrations were measured using the KAPA SYBR Fast qPCR kit (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... Active sites of ATG4A and ATG4B were mutated using QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523) and primer pairs AM 622/AM 623 and MA 624/625 ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were checked by Qubit™ dsDNA Quantification Assay Kits and TapeStation D1000 reagents (Agilent; #5067-5583), and ran on a MiSeq to verify balance before being sequenced on the Illumina NovaSeq S4 (Paired-end ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA integrity was analyzed using Bioanalyzer 2100 and the Agilent bioanalyzer small RNA kit (Agilent, #5067-1548). Example bioanalyzer traces are provided in supplementary figure 2.
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bioRxiv - Genomics 2024Quote: ... The quality of the cDNA and final library preps was assessed using DNA HS bioanalyzer kits (Agilent), and concentration was estimated using a Qubit fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Constructed libraries were analyzed by 2100 Bioanalzyer using High Sensitivity DNA kit (Agilent Technologies, Cat# 5067-4626), and quantified by qPCR using KAPA Library Quantification Kits (Kapa Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... mutations were incorporated into PLEKHA4 and PLEKHA5 plasmids using the QuikChange XL Site-directed mutagenesis kit (Agilent). A nuclear export signal with the amino acid sequence LQLPPLERLTLD was inserted either between PLEKHA5 and GFP or downstream of GFP into the PLEKHA5H-BP-PH-EGFP plasmid ...
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bioRxiv - Biochemistry 2024Quote: ... Point mutations were inserted into the SERF gene by site-directed mutagenesis with a QuikChange kit (Agilent) and were verified by Sanger DNA sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... Additional mutations in this construct were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent technologies). The sequence of the full-length TgREMIND or the [495-840] region of the protein (REMIND region ...
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bioRxiv - Genetics 2024Quote: ... Site-directed mutagenesis of NR1H3 was performed using QuikChange Lightning Site-Directed Mutagenesis Kit (210519, Agilent Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole exomic regions were captured using the SureSelect Human All V6 Exon kit (Agilent Technologies, CA, USA), purified using AMPure XP system (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... The individual associated SNPs were introduced by QuikChange® II XL Site-Directed mutagenesis kit (Stratagene, 200523). All clones were confirmed by sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... CXCR4 mutants were generated by PCR using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA) with full-length CXCR4-AcGFP serving as a template ...
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bioRxiv - Cell Biology 2024Quote: ... The additional K198A and D306N were then introduced using the QuikChange II site-directed mutagenesis kit (Agilent). The same kit was used to generate the HA-tagged D159A and H185R SLC39A9 mutants ...
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bioRxiv - Molecular Biology 2024Quote: ... and the quality was evaluated with an Agilent Bioanalyzer system (RNA 600 Nano kit – Agilent, Waldbronn, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Mutagenesis was carried out with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Cat # 210518-5). Mutant gerAB sequences were integrated into the original gerAB locus by a double crossover along with an erythromycin (erm ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 90 minutes in a biotin conjugated secondary antibody (a goat anti-rabbit, a goat anti-mouse or a swine anti-rabbit as appropriate; Dako; diluted 1:500) and for 1h in a peroxidase conjugated extravidin (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3X with TBST followed by incubation with HRP-conjugated secondary antibodies (Rabbit Cytiva/Amersham NA934; Mouse Agilent/Dako P026002-2) for one hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then incubated for 1 hour at room temperature with rabbit anti-glial fibrillary acidic protein (GFAP; rabbit anti-GFAP antibody, 1:300, Dako, Z0334, Carpinteria, CA), rabbit anti-oligodendrocyte specific protein (OSP ...
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bioRxiv - Bioengineering 2019Quote: ... The following primary antibodies were used for immunocytochemistry experiments: rabbit anti-glial fibrillary acidic protein (GFAP) (DAKO, 1:100, Z-0334, Lot #2002332), mouse anti-vascular endothelial (VE)-cadherin (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako, Santa Clara, CA, USA Z0311, 1:400). Following washing ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the coverslips were incubated in donkey anti goat-647 for TRKB and donkey anti rabbit-568 for the RAB-specific antibodies for 45 min at RT and fixed in Dako Fluorescence Mounting Medium (S3023, Dako North America, Inc.). Control coverslips without primary antibody staining were also included.
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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bioRxiv - Cell Biology 2021Quote: ... The elutes were analyzed by Western blotting as above with the following primary antibodies: polyclonal rabbit anti-ubiquitin (Dako, catalog # Z0458, lot # 20016967) at 1:2,000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: All DNA panel and antibody tag libraries were run on a Bioanalyzer High Sensitivity DNA electrophoresis chip (Agilent Technologies, cat. no. 5067-4626) to verify complete removal of primer-dimer products ...
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bioRxiv - Pathology 2020Quote: ... followed by repeating all steps from antigen retrieval through TSA using an antibody against CK7 (1:200, mouse; Dako cat. no. MS-1352) and the FITC fluorophore (Perkin Elmer cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Primary antibodies were diluted 1:4000 for anti-sGCα1 and 1:2000 for anti-sGCβ1 antibody in 3% dry milk in TBST and incubated with nitrocellulose membranes at 4°C over-night following challenge of membranes with secondary goat anti-rabbit antibody (1:2000 in 3% milk in TBST) conjugated to horseradish peroxidase (Dako A/S, Denmark). Immuno-complexes were visualized using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech ...
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bioRxiv - Pathology 2021Quote: The primary antibodies were incubated at the following concentrations in antibody diluent (PBS + 1% BSA (Fisher BioReagents, BP1605-100) with 0.1% Tween™ 20 (Fisher BioReagents, BP337-500): PanCK (Dako, Z0622, 1:200), Cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Neuroscience 2022Quote: ... In all rats, antibodies staining for neurons (Mouse anti-NeuN, MAB377, MiliporeSigma) and astrocytes (Rabbit anti-GFAP, Z0334, Dako/Agilent, Santa Clara, CA) were used ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were processed as described and probed with a set of 264 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA). Each slide was incubated with one specific primary antibody and a negative control slide was incubated with antibody diluent without any primary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with the conjugated antibody for one hour before being subjected to flow cytometry analysis on an ACEA NovoCyte Quanteon analyzer (Agilent, Santa Clara, CA). For xenograft TME cellular profiling and in vivo cancer cell HLA expression analysis ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies bound to proteins were incubated with a secondary antibody labeled with a polymer as part of the Envision + system containing horseradish peroxidase (Dako Cytomation, Gostrup, Denmark). Images of immunohistochemically stained sections were obtained using an optical microscope (BX51 ...
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bioRxiv - Cancer Biology 2023Quote: ... using the clone DO-7 monoclonal antibody (GA61661-2, Dako Omnis, ready-to-use, incubation 20 min, Agilent Technologies, Santa Clara, CA, USA). Antibody detection was performed using EnVision FLEX/HRP (GV80011-2 ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were processed as described and probed with a set of 258 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA). Each slide was incubated with one specific primary antibody and a negative control slide was incubated with antibody diluent without any primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... HLA-DR-and PLP-stained sections were incubated with HRP-labelled anti-mouse antibody (K5007, Dako Real EnVision detection system; Dako, Nowy Sącz, Poland) for 1 hour and immunostaining was visualized with 3’3-diaminobenzidine (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... The antigen retrieval step was repeated followed by second round staining for pan-CK using anti-pan-cytokeratin antibody (AE1/AE3 mouse monoclonal, Dako-Cat# M351501-2) followed by HRP-conjugated anti-mouse secondary antibody polymer (EnVision+ ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated for 1 hour with horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit antibodies (Agilent Technologies, Santa Clara, CA, USA), washed with PBS 0.05% Tween ...
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bioRxiv - Neuroscience 2023Quote: ... a solution with the secondary antibody and peroxidase (AdvanceTMHRP®, Dako, cat#K4068, Glostrup, Denmark; or EnvisionTM Flex+, Dako, cat#K8002, Glostrup, Denmark) was added for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... before permeabilization with 0.5% Triton X-100 in PBS and stained with primary antibodies: anti-Ki67 (M7240, Agilent Dako, Santa Clara, CA, USA) diluted at 1/50 and anti-Poly-ubiquitin purchased from ENZO (ENZ-ABS840 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 1h at RT before overnight incubation at 4°C with primary antibodies: Glial Fibrillary Acidic Protein (GFAP) (1/500 Dako Cat. Number Z0334); AQP4 (1/500 ...
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bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated with primary antibodies against HER-2 (cell signaling, #4290T, 1:200) or EGFR (cell signaling, #4267, 1:50) and αSMA (Agilent, M085129, 1:100) overnight at +4C ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated overnight at 4°C with the respective primary antibodies: (i) polyclonal rabbit anti-GFAP (1:2000; Dako, Cat. No. Z0334); (ii ...
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bioRxiv - Immunology 2024Quote: ... Ready to Use Antibody reagent was added as marker and incubated for 15 min (CD4: Leica Bio systems, clone 4B12; BCL6: Dako / Agilent, Clone PG-B6p). Detection was achieved using Leica DAB Detection Kit (Bond Polymer Refine Detection ...
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bioRxiv - Plant Biology 2020Quote: ... site-directed mutagenesis (SDM) was performed using a QuikChange II Site-Directed Mutagenesis Kit (Agilent, Santa Clara, US). Primer sequences for SDM are included in Table S9.
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bioRxiv - Plant Biology 2020Quote: LiAc yeast transformation was performed as described in the GAL4 Two-Hybrid Phagemid Vector Kits manual (Agilent Technologies). The constructions including either DNA binding domain or activation domain were co-transformed into yeast strain PJ69-4a ...
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bioRxiv - Biophysics 2021Quote: ... Point mutants were engineered into the DHFR gene using QuikChange II site-directed mutagenesis kits (Agilent cat#200523) using primers specified in Appendix 1 ...
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bioRxiv - Biophysics 2021Quote: ... R214Q/K219N/R353Q triple mutation) were generate using the Quik Change II XL Site-Directed Mutagenesis Kit (Agilent) and verified by sequencing the entire cDNA construct ...