Labshake search
Citations for Agilent :
4601 - 4650 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... and V203R mutations were introduced into the LbmGAP cDNA using the QuickChange XL Site-Directed Mutagenesis Kit (Stratagene). The results of in vitro mutagenesis were verified by sequencing.
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bioRxiv - Genetics 2024Quote: ... Libraries were subjected to quality control using the Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626). Libraries were sequenced using the Illumina Novaseq 6000 system with 2 × 150 paired-end kits ...
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bioRxiv - Cancer Biology 2024Quote: Mitochondrial bioenergetics in AML cell lines were performed using the Seahorse XFp Cell Mito Stress Kit (Agilent Technologies) on the Seahorse XFe96 Analyzer ...
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bioRxiv - Genetics 2024Quote: ... Quality control for the libraries was performed using Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626). Libraries were sequenced on Illumina Novaseq 6000 system with 2 × 150 paired-end kits using the following demultiplexing ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA integrity was assessed on a Fragment Analyzer System using the DNF-471 RNA Kit (15 nt) (Agilent). Sequencing-ready libraries were generated from 100ng of input RNA using a QuantSeq 3’ mRNA-Seq Library Prep Kit REV for Illumina (015UG009V0271 ...
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bioRxiv - Biophysics 2024Quote: ... All GαS variants were obtained from this plasmid by site-directed mutagenesis using the QuikChange II kit (Agilent) and primers listed in Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... The integrity of RNA was confirmed by analysis on a 2100 Bioanalyzer RNA 6000 Pico Chip kit (Agilent) using the RNA Integrity Number (RIN ...
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bioRxiv - Molecular Biology 2024Quote: ... H65A and K67A were introduced into the native YmoA in pBAD18 using the Quick Change II kit (Agilent) by following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The NR1C2-P918A was created via site-directed mutagenesis using the QuickChange Site-directed Mutagenesis Kit (Agilent Technologies). NR1C2 plasmid and mutation primers were reacted with PfU turbo and then digested with Dpn1 ...
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bioRxiv - Neuroscience 2024Quote: ... The quality of the libraries was checked on the 4200 Agilent Tapestation with High Sensitivity DNA kit (Agilent); per sample libraries were sequenced on Illumina Novaseq 6000 with target sequencing depths of 30,000 - 65,000 reads per cell.
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bioRxiv - Genomics 2023Quote: ... Native DNA size distribution assessed using Femto Pulse with Genomic DNA 165 kb kit (Agilent FP-1002-0275).
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bioRxiv - Developmental Biology 2023Quote: ... and library size distribution was confirmed using a 2100 Bioanalyzer DNA 1000 Kit (Agilent, Santa Clara, CA, USA). A unimodal curve centered around 300 – 500 bp was scored as a successful library preparation ...
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bioRxiv - Genetics 2024Quote: ... Libraries were subjected to quality control using the Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626). The following demultiplexing was used to sequence libraries using the Illumina Novaseq 6000 system with 2 × 150 paired-end kits ...
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bioRxiv - Developmental Biology 2024Quote: ... The quality of libraries was checked with the with the High Sensitivity DNA Reagents Kit (Agilent; 5067-4626) on a 2100 Bioanalyzer and cDNA concentrations were obtained using Qubit BR dsDNA Assay Kit (Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... Size selection performance was evaluated by the Fragment Analyzer High Sensitivity DNA Kit (Agilent, Santa Clara, California, USA). If the sample still contained fragments outside the target range of 150 bp to 200 bp ...
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bioRxiv - Microbiology 2024Quote: ... The point mutant E963A was prepared by site-directed mutagenesis using QuikChange™ site-directed mutagenesis kit (Stratagene) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The quality of the amplified cDNA was quantified with the BioAnalyzer High Sensitivity DNA kit D5000 (Agilent Technologies) and its concentration was measured using the Qubit Fluorometer ...
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bioRxiv - Systems Biology 2023Quote: ... Qualitative assessment of Visium libraries was conducted with the High Sensitivity NGS Fragment Analysis Kit (Agilent, DNF-474) on a 96-channel Fragment Analyzer (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... The sequence library was prepared using a Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced using a 2500 Illumina with 125-bp paired-end reads (Illumina ...
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bioRxiv - Genomics 2023Quote: Quality and quantification of the Hi-C libraries was assessed using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). Four biological replicates have been pooled in an equimolar manner and subjected to sequencing using the Illumina NextSeq 550 System in a 75bp paired-end setup ...
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bioRxiv - Cell Biology 2023Quote: ... RNA quality was assessed using a 2100 Bioanalyzer instrument and an Agilent 6000 RNA Pico Kit (Agilent Technologies).
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bioRxiv - Genetics 2023Quote: ... Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange II-directed mutagenesis kit (#200523, Agilent Technologies), according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... N14Y and S17F) and Cx30 (T5M) were produced using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and confirmed by DNA sequencing.
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was processed for reverse transcription (RT) using AccuScript High-Fidelity First Strand cDNA Synthesis Kit (Agilent). A mix of anchored-Oligo(dT ...
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bioRxiv - Genetics 2024Quote: ... and the fragments’ profiles were inspected using the Agilent DNA 1000 Kit on a 2100 Bioanalyzer (Agilent Technologies). The 201 sample libraries prepared were sequenced on a single run in an Illumina HiSeq3000 with single-end and 101bp configurations ...
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bioRxiv - Molecular Biology 2024Quote: Glycolysis and Mitochondrial stress tests were performed on confluent HUVECs according to the manufacturer’s kits and protocols (Agilent Seahorse XF glycolysis stress test kit ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 100 mg/ml CHX) and immediately subjected to RNA extraction using absolutely RNA nanoprep kit (Agilent Technologies) with in-column DNase digestion ...
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity was assessed on a Fragment Analyzer System using the DNF-471 RNA Kit (15 nt) (Agilent). Sequencing-ready libraries were produced using QuantSeq 3’ mRNA-Seq V2 Library Prep Kit with UDI following standard procedures ...
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bioRxiv - Microbiology 2023Quote: ... We evaluated the quantity and quality of cDNA on sixfold dilutions with the RNA 6000 Pico kit (Agilent) on an Agilent 2100 Bioanalyzer.
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bioRxiv - Microbiology 2023Quote: ... and the fragment size distribution was evaluated on the Agilent Bioanalyzer using the High Sensitivity DNA kit (Agilent). Thereafter ...
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bioRxiv - Immunology 2023Quote: ... Appropriate purity and size of the mRNA products was confirmed using an RNA tapestation kit (Agilent, #5067-5579). Prior to use in screens or validation experiments ...
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bioRxiv - Immunology 2023Quote: ... DNA quality was assessed using the Agilent TapeStation instrument with the Genomic DNA Screentape reagent kit by Agilent Technologies (Catalog #5067-5365 and #5067-5366) ...
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bioRxiv - Biophysics 2023Quote: ... Mutations in GIRK2 and GIRK4 were introduced using the PfuUltra II Fusion HS DNA Polymerase kit (Agilent Technologies) and verified by DNA sequencing ...
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bioRxiv - Genetics 2023Quote: ... and the RNA concentration was measured with the Agilent 2100 RNA 6000 Nano Kit (Agilent Technologies, Waldbronn, Germany). First-strand cDNA was generated using the FastKing One-Step RTLPCR Kit (TIANGEN Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... Various PXDLS/T-binding motif base mutations were introduced using a commercial site-directed mutagenesis kit (QuikChange; Stratagene). Chimeric gene constructs were constructed by PCR-based approaches with point mutation primers and GFP tag was fused on the C-terminal to make rat Ribeye -EGFP (rRE-EGFP) ...
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bioRxiv - Neuroscience 2023Quote: ... Single amino acid substitutions of GLIC were engineered using the QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) and oligonucleotide primers (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA integrity number (RIN) was measured using 2100 Bioanalyzer Instrument and Agilent RNA 6000 Nano Kit (Agilent Technologies). RNA samples with the RIN scale ranges from 9 to 10 were used ...
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bioRxiv - Neuroscience 2023Quote: ... and quality was assessed with an Agilent RNA 6000 Nano Kit on a 2100 Bioanalyzer (Agilent Technologies, Inc.). RNA-seq libraries were prepared by the Van Andel Genomics Core from total RNA using the KAPA RNA HyperPrep Kit with RiboseErase (v1.16 ...
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bioRxiv - Plant Biology 2024Quote: ... and its concentration and integrity were assessed using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies). All samples showed an RNA integrity number (RIN ...
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bioRxiv - Genetics 2024Quote: ... Candidate variants were introduced using QuikChange II Site-directed mutagenesis kit according to manufacturer’s protocol (Agilent, CA, USA). The mutations were confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... revelated using diaminobenzidine (DAB) chromogen and counterstained with haematoxylin (EnVision Flex HRP High pH Kit, K8002, Agilent Technologies). The stained slides were scanned at 20X magnification using the Aperio AT2 digital slide scanner (Leica Microsystems) ...
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bioRxiv - Bioengineering 2023Quote: ... and the size distribution was confirmed using a High Sensitivity DNA Kit for Bioanalyzer (Agilent Technologies #5067-4626). Libraries were sequenced on an Illumina NextSeq2000 in paired end mode with the read length of 50 nt and sequencing depth of 20 million reads per library ...
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bioRxiv - Neuroscience 2022Quote: ... RNA fragments were analyzed using the RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer instrument (Agilent Technologies) to determine the RNA integrity number (RIN) ...
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bioRxiv - Immunology 2022Quote: ... and assessment of RNA integrity was performed using the RNA 6000 Nano Kit in the 2100 Bioanalyzer (Agilent). Library preparation ...
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bioRxiv - Immunology 2022Quote: ... Libraries quality was assessed using the HS NGS kit on the Fragment Analyzer (Agilent Technologies, Santa Clara, USA).
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bioRxiv - Developmental Biology 2022Quote: ... 5-μm sections were stained with antibodies or control IgG using the EnVision+System-HRP (DAB) kit (Dako North America Inc ...
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bioRxiv - Bioengineering 2022Quote: ... and the size distribution was confirmed using a High Sensitivity DNA Kit for Bioanalyzer (Agilent Technologies #5067-4626). Libraries were sequenced on Illumina HiSeq2500 in single read mode with the read length of 50 nt and sequencing depth of 20 million reads per library ...
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bioRxiv - Genomics 2022Quote: ... and RNA concentration and quality was measured using the Agilent RNA 6000 Pico Kit (Agilent Technologies, 5067-1513) on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from each brain region using the Absolutely RNA Miniprep Kit (Agilent Technologies, Cat. No. 400800), and stored at —80°C [11] ...
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bioRxiv - Immunology 2022Quote: ... Point mutations (e.g., CARD8 S297A, E274R) were generated using the QuikChange II site-direct mutagenesis kit (Agilent, 200523) following the manufacturer’s protocol ...