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Citations for Agilent :
4551 - 4600 of 5718 citations for Rat Cystatin S CST4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... we created all other Gαi3 mutants featured in this work using a QuikChange® Site-Directed Mutagenesis Kit as per manufacturer’s protocol (Stratagene, USA). A variant that is insensitive to ADP-ribosylation by Pertussis Bordetella toxin (PTX ...
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bioRxiv - Genetics 2023Quote: ... after which DNA fragments in a range of 400-700 bp were excised and extracted using Gel Extraction Kit (A&A Biotechnology) The quality and quantity of the libraries were verified with TapeStation system (Agilent) and Qubit 2.0 fluorometer ...
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... The quality control of the final library products was performed using the Qubit Fluorometer and the Agilent 2100 bioanalyzer with a High Sensitivity Kit (Agilent). Library products were pooled to a final concentration of 4 nM ...
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bioRxiv - Microbiology 2023Quote: ... The quality and quantity of RNA were determined by capillary electrophoresis using an HS RNA kit and 5200 Fragment Analyzer (Agilent). For single-end RNA sequencing (RNA-seq) ...
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bioRxiv - Immunology 2023Quote: ... Agilent Seahorse XF Cell Mito Stress Test Kit was used to perform Mitostress test in the presence of 10mM glucose (Agilent) with addition of Oligomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA integrity and quantity were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). A total amount of 1 μg RNA per sample was used as input material for the libraries preparations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sections were washed and incubated with a secondary antibody (Polymer-HRP Anti-rabbit and DAB following kit protocol (DC EnVision + System, HRP; DAKO). Slides were counterstained with hematoxylin ...
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bioRxiv - Neuroscience 2023Quote: ... Generation of GluK2 point mutants was performed by site-directed mutagenesis using the QuickChange Mutagenesis Kit (Stratagene, La Jolla, CA). Forward and reverse primer for creation of mutants were ...
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bioRxiv - Genetics 2023Quote: ... and the point mutations in the PWWP domain of DNMT3B (W263A, D266A, S270P, K276E and K294E) were introduced using the QuikChange II site-directed mutagenesis kit (Agilent). DNMT3BΔN starts from M200 and was generated by PCR ...
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bioRxiv - Genomics 2023Quote: ... R838W and F867L Ephb4 mutations were introduced by site-directed mutagenesis using a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... exome capture was performed on 200 ng DNA using a customised version of the Agilent Human All Exome V5 Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Genetics 2023Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and post library QC was performed using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed as expected ...
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bioRxiv - Cancer Biology 2023Quote: Whole genome paired-end sequencing was performed using the Agilent SureSelect v3 library preparation kit (Agilent Technologies, Santa Clara, CA). Paired-end 150 base pair reads were generated using an Illumina HiSeq 2500 sequencing instrument ...
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bioRxiv - Cell Biology 2023Quote: ... and Pst I restriction sites of the multiple cloning site of pEGFP-Mieap were introduced using QuikChange Site-Directed Mutagenesis Kits (Agilent) with primers Mut-F1 ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial ATP-linked respiration and extra cellular acidification rate was measured by the Seahorse XFe96 Analyzer using the Mito Stress Test kit (Agilent). 4×104 cells were seeded and grown for 24 h n DMEM containing 10 % FBS ...
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bioRxiv - Cell Biology 2023Quote: All TTBK2 mutations were generated by PCR-based site-directed mutagenesis by using QuickChange Lighting Site-Directed-Mutagenesis kit (Agilent) using as a template pENTR/D-TOPO (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... the HA epitope sequence was inserted immediately 3’ to the signal peptide sequence by site directed mutagenesis using the QuickchangeXL site directed Mutagenesis kit (Agilent) to obtain the following intermediate vector ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA libraries were finally assessed for quality with the Agilent 2100 BioAnalyzer using DNA high sensitivity kit (Agilent: 5067-4626) and quantified using Qubit DNA high sensitivity assay (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2023Quote: ... RNAseq analysis was performed by LC Sciences (Houston, TX) for RNA purity/quantity assessment via Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent), mRNA extraction ...
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bioRxiv - Genetics 2023Quote: ... The final libraries were quality checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent, 5067-4626), Qubit dsDNA HS Assay kit (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... The RNA quality was validated using NanoDrop and Agilent DNF-472 HS RNA (15 nt) Kit (Agilent Technologies, CA, USA) on the 5200 Agilent system to confirm the purity ...
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bioRxiv - Genomics 2023Quote: ... Purified RNAs were quantified by Qubit and quality was assessed using RNA Pico Assay kit with Bioanalyzer (Agilent Technologies, USA). chrRNA were then subjected to library preparation using True-seq stranded total RNA library prep gold kit (Ref#220599 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... An A to C mutation was made at base pair 219 within the CAT-I gene using the QuickChange Lightning Site-Directed Mutagenesis kit (Agilent) to match the native E ...
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bioRxiv - Biophysics 2023Quote: ... A clone encoding tagless wild type PUS1 was then generated from PUS1D134A by converting alanine 134 back to wild type aspartate 134 using a QuickChange II XL kit and protocol (Agilent).
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The mRNA was purified and fragmented from a total amount of 1 μg RNA per sample ...
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bioRxiv - Cancer Biology 2022Quote: Percent ATP produced by mitochondria and the level of compensatory glycolysis were measured using a Seahorse XF Real-Time ATP Rate Assay Kit and XFe96 Analyzers (Agilent) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The total RNA yield was measured with a Nanodrop and the RNA integrity values (RIN) were obtained with a Bioanalyzer 2100 (Pico RNA 6000 Kit, Agilent). The values obtained for each of the samples are shown in Supplementary Table S1.
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bioRxiv - Immunology 2023Quote: ... following the manufacturer’s instructions and were run on an Agilent 2100 Bioanalyzer using an Agilent High Sensitivity DNA kit (Agilent Technologies) to ensure the proper range of cDNA length distribution ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 µg DNase-treated RNA was reverse transcribed using a cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA), following the respective manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Bioengineering 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Genetics 2023Quote: ... Point mutations in KCNJ1 were introduced into the resulting pRS415TEF1-ROMK1 vector using either two-step overlap extension mutagenesis (141) or site-directed mutagenesis with the QuikChange kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Point mutants of the vectors were constructed by site-directed mutagenesis using a QuikChange Site-Directed Mutagenesis kit (Stratagene, CA) (Yoshida et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... according to manufacturer’s instructions and the quality was assessed with the Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent Technologies). PolyA-enriched RNA libraries were prepared with the KAPA Stranded mRNA Sample Preparation Kit (Kapa Biosystems).
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bioRxiv - Cancer Biology 2023Quote: ... The mutagenic primers were designed using QuickChange primer design program at www.agilent.com/genomics/qcpd according to the guidelines mentioned in the manufacturer’s protocol (QuickChange Lightning Site-Directed Mutagenesis kit, Cat# 210518, Agilent Technologies). This was followed by mutant strand synthesis and transformation was performed as mentioned in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 50 μM 2-deoxy-glucose (2-DG) + 1μg/μL Hoechst (Seahorse XF Glycolysis Stress Test Kit, Agilent, 103020) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The concentration and quality of the synthesized MPXV mRNA were measured using an Agilent 2100 Bioanalyzer and RNA Nano 6000 Assay Kit (Agilent), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... DNA integrity was evaluated with a Fragment Analyzer (Advanced Analytical Technologies) using the HS NGS Fragment Kit (DNF-467, Agilent). dsDNA HS assay (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... UHOR_02675 active site mutants (Ribo1-ME80Q/D98N) were created from by using QuickChange XL Site-Directed Mutagenesis Kit from Agilent Technology according to manufactures instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and run on the Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA Kit (Agilent Technologies, Santa Clara CA, USA) to confirm amplicon length and purity ...
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bioRxiv - Genetics 2023Quote: ... PREPLs was cloned into the bacterial expression vector pVP68K and mutations were introduced using the QuikChange II site directed mutagenesis kit (Stratagene) and confirmed by direct Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... The quality and size distribution of the DNA was assessed on a Fragment Analyzer using the HS NGS Fragment Kit (Agilent). The pooled samples were then sequenced on a NovaSeq 6000 sequencer (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Transcribed cDNA was diluted ten-fold and used for real-time quantitative PCR (QuantiTect SYBR Green PCR Kit; Quiagen; 204145) performed with the MX3005P thermocycler (Stratagene).
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bioRxiv - Cell Biology 2024Quote: ... Point mutations in Cebpa p42 were introduced by site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene #200518). Primers used for single mutant exchange were ...
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bioRxiv - Cell Biology 2024Quote: Mitochondrial bioenergetics of MDMs and DCs were measured using the Seahorse XF Cell Mito Stress Test Kit and the XF24 Extracellular Flux Analyzer (Agilent), as previously described (100) ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer’s instructions ...