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Citations for Agilent :
401 - 450 of 2432 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Samples were acidified with TFA to a pH < 2 and desalted by C18 using the Bravo AssayMAP platform (Agilent) before enrichment of crosslinked peptides with Fe(III)-NTA cartridges in the Bravo AssayMAP platform as previously described[23].
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bioRxiv - Microbiology 2023Quote: ... ammonia and separated using high pH reverse phase Zorbax 300 extend C-18 4.6 mm x 250 mm (Agilent) column and liquid chromatography AKTA Micro system (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed in DAKO PT Link in a DAKO Target Retrieval (Low pH) Solution (DAKO, Cat# S1699) at 98°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Both TMT were fractionated in a basic pH reversed phase chromatography using a HPLC equipped with a 3.5 µm Zorbax 300 Extended-C18 column (Agilent). Fractions were collected in a 96-well plate ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture media was replaced with assay media (Seahorse XF DMEM medium pH 7.4, Cat. No. 103575-100, Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture media was replaced with assay media (Seahorse XF DMEM medium pH 7.4, Cat. No. 103575-100, Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently fractionated though basic pH reversed phase chromatography using a HPLC equipped with a 3.5 µm Zorbax 300 Extended-C18 column (Agilent). The fractions were first collected into a 96-well plate and then combined into 24 samples ...
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bioRxiv - Cancer Biology 2023Quote: ... used a two solvent gradient (Mobile Phase A: 10 mM Ammonium Acetate in 90/10 Water/Acetonitrile, pH 9.2, 5 μm Agilent InfinityLab Deactivator Additive ...
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bioRxiv - Neuroscience 2024Quote: ... Selected de-waxed brain paraffin sections containing dorsal hippocampus were treated with DakoTarget retrieval solution (pH 9) (Dako, Denmark) at 95° in a Dako PT Link to retrieve protein antigenicity ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibody dilutions were prepared in antibody diluent (Agilent, London, UK). The primary antibodies and concentrations used are as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were diluted in Dako Real Antibody Diluent (Dako, S2022). Staining was performed on the BenchMark XT immunostainer (Ventana Medical Systems).
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted in Dako antibody diluent (S0809, Dako) and incubated on sections overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted in Dako antibody diluent (S0809, Dako) and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Anti-iba1 microglial antibody (AlphaLaboratories) or anti-GFAP antibody (Agilent) were used with biotinylated polyclonal goat anti-rabbit immunoglobulin secondary antibodies (Dako ...
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bioRxiv - Cancer Biology 2024Quote: ... primary antibodies were typically diluted in antibody Diluent (DAKO, S2022) with 2% milk (v/v ...
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bioRxiv - Physiology 2019Quote: ... Secondary antibodies (Dako) anti-mouse ...
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bioRxiv - Biochemistry 2020Quote: ... Antibodies: Ubiqutin (Dakocytomation); Cdc53/yCul1 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies (Dako Donkey anti-goat P0449 ...
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bioRxiv - Developmental Biology 2023Quote: ... CD3 antibody (Dako M725429-2 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were pooled in a 1:1 ratio and then separated into 96 fractions on a 250 mm C18 column via high-pH reverse-phase LC (Agilent) with a gradient shifting over the course of 120 minutes from ammonium formate and 2% ACN Buffer A to ammonium formate and 90% ACN buffer B ...
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bioRxiv - Cell Biology 2020Quote: ... Organoids treated with PBS control or IL-1β were added with dispase to remove Matrigel and washed twice with XF Base Medium (DME, pH 7.4) supplemented with 1mM glutamine (Seahorse Bioscience). 30,000 cells were seeded on each well and incubated for 1hr at 37°C in non-CO2 incubator before measurement ...
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bioRxiv - Cancer Biology 2019Quote: ... the slides were incubated in citrate buffer (pH 6.0) in a pressure cooker (Pascal, Agilent Technologies, Santa Clara, CA, USA). The endogenous peroxidase was blocked with a commercial solution (Protein Block ...
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bioRxiv - Cancer Biology 2020Quote: ... France) Citrate buffer pH6 was used for antigen retrieval 20min à 96°C (Target Retrieval so-lution low pH, Dako) and DAB and Hematoxylin staining were revealed using ImPath detection kit (DAB OB Sensitive Detection Kit ...
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bioRxiv - Cell Biology 2020Quote: ... the formalin-fixed and paraffin-embedded bronchial organoid samples were treated with pH 6.0 citrate buffer for 30 sec at 125°C in a pressure cooker (Dako Japan) as antigen retrieval ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subjected to antigen retrieval antigen retrieval (pH = 6.0 for cleaved caspase-3 and pH = 9.0 for cleaved PARP-1) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Cancer Biology 2021Quote: ... while pH = 9.0 was used for cleaved PARP-1) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Cancer Biology 2022Quote: ... FFPE sections were loaded into an Agilent pre-treatment module to be dewaxed and undergo heat induced epitope retrieval (HIER) either using low or high pH target retrieval solution (TRS) (K8005, K8004, Agilent). Sections for αSMA staining underwent antigen retrieval using low pH TRS ...
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bioRxiv - Biophysics 2022Quote: ... was pre-equilibrated with 10 mM pH 7 sodium phosphates with 150 mM NaCl on an Infinity 2 1260 HPLC system (Agilent) with an inline degasser ...
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bioRxiv - Immunology 2022Quote: ... approximate ~1 ×105 TH17 cells were suspended in 50 μL assay medium (Seahorse XF RPMI Assay Medium, pH 7.4, Agilent Technologies) containing 10 mM glucose ...
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bioRxiv - Neuroscience 2022Quote: ... Human brain sections were rehydrated and antigen demasking was done using EnVisionTM FLEX Target Retrieval Solution (Agilent Technologies, pH 9.0) under heat ...
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bioRxiv - Bioengineering 2022Quote: Retinal cross-sections after deparaffinization were treated with target retrieval solution at 99 °C for 1 h (pH 6.1; Dako #S1699) and washed with 1x PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to antigen retrieval (Sodium Citrate 10mM, pH=6.0) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to antigen retrieval (Sodium Citrate 10mM, pH=6.0) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Cell Biology 2021Quote: ... samples were offline fractionated over 90 minutes and separated into 96 fractions by high pH reverse-phase HPLC (Agilent LC1260) through an Agilent ZORBAX 300Extend C18 column (3.5-μm particles ...
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bioRxiv - Cell Biology 2022Quote: ... and extracellular acidification rates (ECAR) were measured in XF DMEM assay medium with pH adjusted to 7.4 (103680-100, Agilent Technologies) containing 10 mM glucose (103577-100 ...
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bioRxiv - Cancer Biology 2019Quote: ... MSH2 and PMS2 and at pH 9 for MSH6) using a detection system suitable for the Dako Autostainer Link 48 (EnVision FLEX, Dako).
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bioRxiv - Developmental Biology 2020Quote: ... IHC followed a routine protocol using a PT link antigen retrieval module and high pH antigen retrieval solution (both Dako Pathology/Agilent Technologies ...
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bioRxiv - Zoology 2020Quote: ... Slides were rinsed with PBS (pH = 7.4) and then subjected to antigen retrieval procedure using proteinase K 1:30 (Dako, Denmark). Nonspecific binding sites were blocked with 2% BSA for 30 min in a humid chamber at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Antigen retrieval was performed either with Proteinase K (for F4/80) or boiling the sections in a high pH antigen retrieval buffer (Dako Target retrieval Solution ...
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bioRxiv - Cell Biology 2020Quote: ... followed by washes in buffered Seahorse Assay medium (pH 7.4) and subsequent metabolic testing according to the manufacturer’s protocol (Agilent Seahorse XF96). Oligomycin (2 μM ...
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bioRxiv - Physiology 2021Quote: ... pH (using the indicator dye purified m-cresol purple [34] in an Agilent 8453 spectrophotometer (Agilent, Santa Clara, CA, USA)) ...
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bioRxiv - Immunology 2021Quote: ... Epitope retrieval was performed at 97C for 10 min with the Dako Target Retrieval Solution pH 9 (Agilent, S236784-2) on a Lab Vision PT Module (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... and antigen retrieval was performed by heat-induced epitope retrieval (HIER) in Dako PT Link with a high pH buffer (Dako). The staining was performed with an autostainer (Autostainer Link 48 ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: The gene for Pyrococcus horikoshii (Ph) LeuRS was synthesized by Gene Universal and expressed from pET-11a in BL21-CodonPlus (DE3)-RIPL (Agilent). Cells were grown at 37°C and induced with 300 µM IPTG for 4 hours then harvested and stored overnight at -20°C ...