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Citations for Agilent :
401 - 450 of 597 citations for Herpes Simplex Virus gG2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Proteins were expressed in and purified from BL21-CodonPlus(DE3)pLysS Escherichia coli (Agilent Technologies, Santa Clara, CA) as described 48 and dialyzed against PLS buffer ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 14,000 rpm on a tabletop centrifuge to remove aggregated proteins and then sequentially injected into a HPLC (Agilent) equipped with a C18 analytical column (Agilent Poroshell 120).
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bioRxiv - Biophysics 2022Quote: ... The UV–visible spectra of purified NQO1 proteins were measured in a Cary spectrophotometer (Agilent Technologies, Waldbronn, Germany) and used to quantify NQO1 concentration and the content of FAD as described in (12) ...
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bioRxiv - Synthetic Biology 2022Quote: The Cas12a library targeting the protein-coding genes in PO1f was ordered as an oligonucleotide pool from Agilent Technologies Inc ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Abs) against glial fibrillary acidic protein (GFAP, rabbit anti-GFAP (1:2000, Dako, Z0334, RRID: AB_10013382)) and neuronal nuclear antigen (NeuN ...
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bioRxiv - Immunology 2022Quote: ... C antibody (clone W6/32) that was immobilized and covalently linked to Protein A cartridges (Agilent G5496-60000). Peptides were acid eluted from antibody bound ProteinA cartridges using 0.1M acetic acid/0.1%TFA followed by desalting with C18 solid-phase extraction (SPE) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a rinse with TBS and a 30-minute blocking step with the Dako Protein Block (Dako) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Antigens were tetramerized by incubation at a >4:1 ratio of biotinylated protein with streptavidin-PE (Agilent; PJRS25) or streptavidin-APC (Agilent ...
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bioRxiv - Neuroscience 2023Quote: Free-floating brain tissue was stained for polyclonal rabbit anti-glial fibrillary acidic protein primary antibody (GFAP; DAKO, Santa Clara ...
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bioRxiv - Bioengineering 2023Quote: ... cryosections of the tissues were first permeabilized and blocked using a protein block solution (Dako, Carpinteria, CA, USA) containing 0.1% saponin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2023Quote: SECMALS analysis of protein nanoparticles was performed on a 1260 Infinity II high-performance liquid chromatography system (Agilent) coupled with a miniDAWN and Optilab detectors (Wyatt Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were passively cooled to room temperature and then incubated in Protein Block Serum-Free Reagent (Agilent (Dako), Santa Clara ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were passively cooled to room temperature and then incubated in Protein Block Serum-Free Reagent (Agilent (Dako), Santa Clara ...
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bioRxiv - Molecular Biology 2024Quote: The purified IMP2 protein +/− RNA was analyzed by SEC-MALS using an HPLC system (Agilent Technologies 1260 Infinity) connected to a MALS system (Wyatt DAWN HELEOS II Ambient with Optilab TrEX HC differential refractive index detector) ...
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bioRxiv - Biochemistry 2022Quote: ... 50 μl of each protein sample at 8.5-10 mg/ml was injected onto a BioSEC3-300 column (Agilent) at a 0.2 ml/min flow rate ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used were rabbit anti-cow glial fibrillary acidic protein (GFAP; 1:5000; Z0334; Dako, Carpinteria, CA, USA) to detect astrocytes ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were revealed by chemi-luminescence (Super Signal West Dura Substrate, Pierce) using a secondary peroxydase-conjugated antibody (Dako) at a dilution of 1:10000 ...
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bioRxiv - Immunology 2021Quote: ... for 10 minutes at 37°C for F4/80 or blocked with a protein block (catalog number x0909, Dako) for 10 minutes followed by an Fc block (catalog number 553142 ...
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bioRxiv - Cancer Biology 2021Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... as described previously (11) or by using Protein G cartridges on the AssayMAP Bravo (Agilent Technologies, Santa Clara, USA) Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... About 1.0 µg of protein for each sample was injected, bound to a PLRP-S column (2.1mm x 50mm, 5μm, 300Å) (Agilent Technologies), desalted ...
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bioRxiv - Microbiology 2021Quote: ... The myr(–)MA and myr(–)MA99 proteins were overexpressed in Escherichia coli BL21 Codon Plus-RIL cells (Agilent Technologies). Cells were grown in LB broth supplemented with 100 mg/L Ampicillin at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... the separation of digested protein samples was performed on an Agilent 1290 Infinity HPLC system (Agilent Technologies, Waldbronn, Germany) coupled to a Q Exactive™ HF (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The digested proteins were desalted using AssayMap C18 cartridges mounted on a BRAVO liquid handling system (Agilent, Columbia, MD), and the organic solvent was removed in a SpeedVac concentrator prior to LC-MS/MS analysis.
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bioRxiv - Biochemistry 2021Quote: ... These data were then normalized to the corresponding DNA/protein/cell number in the Wave data analysis software (Agilent) and exported to Microsoft Excel.
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bioRxiv - Immunology 2021Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The final protein concentration was measured using ultraviolet-visible (UV-vis) spectroscopy on a Cary 100 system (Agilent Technologies). All proteins were aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... 100 μl of each protein sample at 1.7-36 mg/ml were injected onto a BioSEC3-300 column (Agilent), run at a 0.3 ml/min flow rate ...
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bioRxiv - Biochemistry 2022Quote: ... The protein sample is scanned at room temperature using fluorometer (Cary Eclipse Fluorescence Spectrophotometer, Agilent, Santa Clara, CA, USA). During the scan ...
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bioRxiv - Cancer Biology 2022Quote: ... The nonspecific-binding sites on sections were then blocked using Protein Block Serum-Free reagent (Dako North America Inc.) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The tetrameric detection reagents were generated immediately prior to use by combining biotinylated FcɣR proteins (Ackerman Laboratory) with a 1/4th molar ratio of Streptavidin-Allophycocyanin (ProZyme) and mixing by inversion for 10 min ...
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bioRxiv - Immunology 2023Quote: Lung homogenate protein concentrations were determined using a Bio-Rad DC protein assay performed according to the manufacturer’s protocol and read on a Synergy H1 plate reader (Agilent). Concentrations were calculated by extrapolation from a BSA standard curve using Gen5 v.3.00 (Agilent) ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration in the host fraction was measured on a spectrophotometer (BioTek PowerWave XS2, Agilent, Santa Clara CA, USA) using Coomassie Plus Bradford assay reagent (Pierce ...
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bioRxiv - Synthetic Biology 2023Quote: Protein concentrations were obtained by measuring absorbance at 488 nm on a Cary 60 UV-Vis spectrophotometer (Agilent Technologies) and calculating concentrations using Beer’s Law ...
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bioRxiv - Biophysics 2023Quote: ... A non-cleavable T855G mutant of this Lphn3 GAIN domain fusion protein was generated using QuikChange II XL (Agilent) site-directed mutagenesis with primers 5’-ATG CAG CTG TAA TCA CCT GGG CAA CTT TGC TGT CCT GAT G -3’ and 5’-CAT CAG GAC AGC AAA GTT GCC CAG GTG ATT ACA GCT GCA T -3’.
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bioRxiv - Cancer Biology 2023Quote: ... The protein (10 μL) was loaded by an LC system (Agilent 1290 Infinity II, Agilent Technologies, Santa Clara, CA) on a HPLC column (PLRP-S 1000 Å ...
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bioRxiv - Physiology 2023Quote: ... Secreted protein in cell culture media was enriched using StrataClean Resin (Agilent, 10 µl per 5 ml of media) as previously described11,24.
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bioRxiv - Immunology 2023Quote: ... the slides were washed in 1× PBS and then exposed to Dako Serum- free Protein Block solution (Agilent, USA) for 45Lmin at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were incubated for 30 minutes at room temperature prior to injection onto an HPLC (Agilent, 1260 Infinity II). 5 μL of protein complex was injected onto a MAbPac 4 x 300 mm SEC column with 5 µm particle size and 300 Å pore size ...
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bioRxiv - Immunology 2024Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Following permeabilization the cells were washed three times with PBS then blocked with Protein Block (Aligent/Dako, X090930-2). Primary antibodies (ADAR1 (Santa Cruz ...