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Citations for Agilent :
401 - 450 of 799 citations for 7α 12α Dihydroxycholest 4 en 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... then sections were incubated overnight with primary antibodies at 4°C with Background Reducing Components (DAKO). Alexa Fluor secondary antibodies (Abcam ...
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bioRxiv - Genetics 2021Quote: ... in PBS for 20 minutes at 4°C before mounting with Dako Fluorescence Mounting Medium (Agilent) and glass coverslips ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated at room temperature for 4 h and separated by HPLC using a Bondapak column (Agilent) as described previously(Mosharov et al. ...
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bioRxiv - Biochemistry 2021Quote: Oxygen consumption rate (OCR) was measured using Seahorse XFe96 analyzer (Agilent, with Wave 2.3/4 software) and the Seahorse XFe96 FluxPak (102416-100 ...
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bioRxiv - Physiology 2021Quote: HUVECs (passages 4-6) were seeded in XFe 24 well plates (Agilent, Santa Clara, CA, USA) at 30,000 cells per well in 200 µL EGM for 48 h ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were incubated overnight (4 °C) with rabbit anti-GFAP (1:500; DAKO, Z0334). The antibody was prepared in 10% donkey serum in PBS containing 0.02% sodium azide and 0.3% triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were blotted with the following primary antibodies: anti-insulin (IR00261-2, 1:4; Agilent Techologies) and anti-glucagon (g2654 ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were then quantified using Qubit™ Fluorometer 4 and Fragment analyzer 5200 (Agilent, CA, USA) to ensure the average final insert sizes of 450–550 bp ...
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bioRxiv - Microbiology 2023Quote: ... All plates had lids and were piled up and inserted to a stacker (BioStack 4, Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... at 4 °C ON and afterward incubated with rabbit anti-HA antibody 1:2000 (Dako, A0001) 2 h at RT ...
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bioRxiv - Biophysics 2020Quote: ... Optical absorption spectra of the samples in a 300-900 nm range were recorded within about 10 s at given time intervals (about 0.3-1.0 min) in a 3 mL quartz cuvette (Helma QS1000, 1 cm pathlength) using a Cary 60 spectrometer (Agilent). Alternatively ...
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bioRxiv - Genetics 2019Quote: ... 3 μg of purified RNA were converted into cDNA via the Affinity Script QPCR cDNA synthesis kit (Agilent Technologies). cDNA synthesized without the RT/RNase block enzyme mixture was utilized as a control for genomic DNA contamination ...
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bioRxiv - Systems Biology 2019Quote: ... sections were incubated with 3% H2O2 for 20 minutes to inactivate endogenous peroxidases (Dako EnVision+ System-HRP-DAB, K4010) and subsequently treated with 10% normal goat serum (Dako ...
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bioRxiv - Microbiology 2019Quote: ... 3 µg of purified RNA were converted into cDNA via the Affinity Script QPCR cDNA synthesis kit (Agilent Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... analyte separation was achieved using a Synergi Hydro-RP column (2.5 µm 3×100mm, Phenomenax) at ambient temperature on a 1260 Infinity (Agilent)-QTRAP500 (AB Sciex ...
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bioRxiv - Genomics 2022Quote: ... RT was performed with a specific primer (5′-CCTACACGACGCTCTTCC-3′) using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). RNA degradation was performed by incubating the RT mixture with 10% 1 M NaOH (2μl of RT mixture ...
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bioRxiv - Immunology 2020Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97 °C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97 °C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... From each sample 10 µl was injected onto a SEC-3 HPLC column (300 Å pore size; Agilent Technologies) equilibrated with HKMC at a flow rate of 0.3 ml/min ...
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bioRxiv - Biochemistry 2021Quote: HMECs were cultured at a density of 3 x 104 cells/well in 24-well Seahorse XFe24 plates (Agilent) and incubated at 37 °C under a humidified 5% CO2 atmosphere overnight in the presence or absence of DMF ...
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bioRxiv - Pathology 2021Quote: ... Tissues next underwent antigen retrieval by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: ... Tissues next underwent antigen retrieval by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Polyamines were extracted via gravity alone (nominal flow rate of 0.07 mL/min) onto a 1000 mg Bond Elut-C18 SPE column (3 mL, Agilent) pre-conditioned with methanol and bicarbonate buffer at pH 12 ...
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bioRxiv - Genetics 2020Quote: ... Baseline respiration and maximal respiratory capacity were measured using the Seahorse instrument (XF24 data for Figure 2 and Xe96 data for figure 3) according to manufacturer’s instructions (Agilent). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... or 100μM W-7 for 30m prior to application of vehicle or 100μM NMDA for 3-5m before crosslinking at 254nm in a Stratalinker 2400 (Stratagene) at 1500mJ/cm2 ...
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bioRxiv - Microbiology 2022Quote: ... The plate was then exposed to a temperature gradient for 3 min in a PCR machine (Agilent SureCycler 8800), followed by 3 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... 5 μL of the sample were injected into a C18 reversed phase column (InfinityLab Poroshell 120 EC-C18 2.7 μm 3×100 mm, Agilent) at 40C and detected with an HPLC fluorescence detector (1260 Infinity II Fluorescence Detector ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Size distribution and concentration of 3’ cDNA libraries were verified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). Finally ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Cyanine 3-labelled cRNA was synthesized from 150 ng RNA using the Low Input Quick Amp Labeling Kit (Agilent) and predicated using RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... Myotubes grown on 24 well plates were fasted for 3 h in glucose-free DMEM (103575-100, Agilent, USA). Incubation with 100 nM insulin (Roche Diagnostics ...
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bioRxiv - Neuroscience 2019Quote: Genome-wide expression profiling was performed using the Zebrafish (v3) Gene Expression 4×44K Microarray (Agilent G2519F) containing 43803 probes according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... the plasma was diluted 4:1 with Buffer A for Multiple Affinity Removal LC Columns (Agilent Technologies) and filtered through a 0.22 μm hydrophilic PVDF membrane filter plate (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... The (Cy3)-cRNA samples were hybridized to Agilent mouse 4 × 44 K-format oligonucleotide microarrays (Agilent Technologies). The slides were washed according to the manufacturer’ s instructions and scanned with an Agilent DNA microarray scanner.
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bioRxiv - Neuroscience 2022Quote: ... They were then incubated overnight at +4 °C with the 6F3D anti-Aβ antibody (Dako, 1/200), polyclonal anti-tau antibody (Dako ...
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bioRxiv - Neuroscience 2022Quote: ... P1.SCPN (4)) were evaluated for quality on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), quantified using Qubit 2.0 Fluorometer (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... was applied at 4°C overnight and secondary polyclonal goat anti-mouse-HRP (Agilent P0447; 1:1,000) was applied for 1 h at room temperature ...
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bioRxiv - Pathology 2021Quote: ... OD600 was measured every hour for 24 hours in a BioTek Synergy 4 microplate reader (BioTek-Agilent). In vitro growth graphs were generated using ggplot2 and ggprism ...
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bioRxiv - Physiology 2023Quote: ... The plate was immediately loaded into a Biotek Synergy 4 96-well plate reader (Agilent Technologies, USA), and NADPH fluorescence (340/460 excitation/emission ...
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bioRxiv - Microbiology 2023Quote: ... TIMS dimension was calibrated linearly using 4 selected ions from ESI Low Concentration Tuning Mix (Agilent Technologies) [m/z ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C with anti-total tau primary antibody solution (1: 5000, DAKO A0024). After 1 hour incubation at RT in the corresponding secondary antibody solution (donkey anti-rabbit 800) ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunohistochemical reaction was developed using 3, 30-diaminobenzidine tetrahydrochloride (DAB) or Purple Kit (Chromo Map DAB or Purple Kit, Ventana, Roche; DAB (Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
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bioRxiv - Cell Biology 2020Quote: ... Frozen samples were spread to a thin layer on petri dishes pre-cooled on dry ice and UV-crosslinked 3 times at 400 mJ/cm2 using a Stratalinker 2400 (Stratagene). All samples were stored at −80°C until tissue lysis.
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 drops per slide of secondary antibody (EnVision + labeled polymer-HRP anti-mouse, Dako K4001, or anti-rabbit, Dako K4003) were added and incubated for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific point mutations in Hmga1 3’UTRs (shown in Fig 3A) cloned into pRL-CMV vector were generated using site-directed mutagenesis kit (Stratagene). In addition ...
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bioRxiv - Molecular Biology 2020Quote: Per2AS variant 2 overexpression plasmid was generated from 5’RACE and 3’RACE products that were cloned in to pBluescript (Agilent), as well as Per2AS qPCR product cloned into pGEM-T (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... Before separation peptides were first trapped (Dr Maisch Reprosil C18, 3 μm, 2 cm x 100 μm) and then separated on an analytical column (Agilent Poroshell EC-C18 ...
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bioRxiv - Developmental Biology 2020Quote: ... the included linker sequence between 3’ UTR and the start of sfGFP was removed using site-specific mutagenesis (QuikChange, Agilent). The gRNA expression plasmid and donor plasmid were injected by BestGene into embryos of Vas-Cas9 flies (#56552 ...