Labshake search
Citations for Agilent :
4251 - 4300 of 5930 citations for Rat N acylethanolamine hydrolyzing acid amidase NAAA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Final and cDNA libraries were then QCed on a bioanalyzer (Bioanalyzer High Sensitivity DNA kit: Agilent Technologies, Inc., 5067-4626) and qubit (dsDNA high sensitivity kit ...
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bioRxiv - Cell Biology 2020Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Constructs for BaxT182I and G179P were generated with a QuickChange site-directed mutagenesis kit (#210518; Agilent Technologies, Santa Clara, CA) on the pTYB1 WT-human Bax plasmid (Suzuki et al. ...
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bioRxiv - Genomics 2019Quote: The mutagenesis reactions to remove the Adenosine nucleotides near the cleavage sites were carried out using the QuikChange Site-Directed Mutagenesis Kit (Agilent). The mutagenesis DNA primers for the site mutation reactions are available in Supplemental Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... at 4 °C overnight and immunostained using the streptavidin-biotin peroxidase technique (Envision universal peroxidase kit; Dako Cytomation, Milan, Italy). After incubation ...
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bioRxiv - Cancer Biology 2019Quote: ... were quantified using KAPA library quantification kits (KAPA) and fragment size distributions were determined using a Bioanalyzer or Tapestation (Agilent). Sequencing was performed on a HiSeq 4000 (Illumina).
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bioRxiv - Plant Biology 2020Quote: ... Library validation included size and purity assessment with the Agilent 2100 Bioanalyzer and the Agilent DNA 1000 kit (Agilent Technologies). Samples were diluted to 10nM and pooled to create the 12X multiplexed DNA libraries ...
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bioRxiv - Biochemistry 2019Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The RNA concentration was measured with an RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... the kaiC36803 gene in the pGEX-kaiC36803 vector was modified by site directed mutagenesis using the Quick-Change Site-Directed Mutagenesis Kit (Stratagene) or Q5 Site-Directed Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2019Quote: ... Agilent gene expression microarray 60K slide (Design ID: 72363. SurePrint G3 Human Gene Expression 8 × 60K Microarray Kit, Agilent Technologies) were used ...
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bioRxiv - Developmental Biology 2019Quote: ... Mutations in the miR-204 binding sites were generated using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA) and the mutated sequences were confirmed by DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and fragment size was checked using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed normally about 500 bp ...
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Microbiology 2019Quote: ... Fragment size distribution was analyzed by capillary gel electrophoresis using Agilent High Sensitivity DNA kit in a Bioanalyzer 2100 instrument (Agilent) then indexed sequencing libraries were prepared using TruSeq Nano DNA LT kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... The quality of the library was checked on DNA high sensitivity assay kit on Bio-analyser 2100 (Agilent Technologies, USA) and were sequenced on the Ion S5 Plus sequencing platform using 530 chip.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Deletion and site-directed mutants were generated in human ENaC cDNAs cloned into a pGEM HE vector using the QuikChange II Site-Directed Mutagenesis kit (Stratagene) (Ji & Benos ...
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bioRxiv - Cancer Biology 2020Quote: ... The DNAJB1-PRKACA K128H kinase-dead mutant was cloned using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using the following primers (5’-CCTTCTGTTTGTCGAGGATATGCATGGCATAGTGGTTCCCG-3’ and 5’-CGGGAACCACTATGCCATGCATATCCTCGACAAACAGAAGG-3’) ...
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bioRxiv - Developmental Biology 2019Quote: Pkin-29::kin-29SER517ALA was generated by modifying Pkin-29::kin-29cDNA using PCR-based mutagenesis (Quickchange II XL site-directed mutagenesis kit, Stratagene). The following primers were used ...
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bioRxiv - Molecular Biology 2020Quote: ... the gene of FASTD71V,P73T was randomly mutagenized by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent). The PCR product was digested with NheI and BamHI ...
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bioRxiv - Cell Biology 2019Quote: ... The HsMyo1e E337S TEDS site mutation was generated in pBS-HsMyo1e construct using the QuickChange kit (Stratagene; La Jolla, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Isolated RNA was reverse transcribed into cDNA was by Prime Script TM RT reagent Kit with gDNA Eraser (Stratagene, Takara). Real-time PCR was performed on ABI-7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... For quality control libraries were analyzed with a DNA High Sensitivity Kit on a 2100 Bioanalyzer system (Agilent Technologies, USA) and again quantified on a Quantus™ Fluorometer (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... The KEN-box mutant was generated in the pMT-Cyclin B1-GFP plasmid using a site-directed mutagenesis kit (Agilent), following the manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: Desired mutations were introduced into mhsT wild-type gene via site-directed mutagenesis (Mutagenesis QuikChange Lightning Kit, Agilent Technolgies, Inc) using designed primers ...
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bioRxiv - Microbiology 2019Quote: ... Spike-in RNAs were added to 100 ng total RNA using the One-color RNA Spike-In kit (Agilent Technologies) and Cy3-labeled cRNAs were synthesized using the Low Input Quick Amp Labeling kit (one-color ...
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bioRxiv - Developmental Biology 2019Quote: ... The total RNA quantity and purity were analyzed on Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Systems Biology 2020Quote: ... After double XP beads purifications (with beads added in a ratio 1:1) libraries were quantified on a Fragment Analyzer run with a NGS Assay Kit (Agilent) and loaded on a HiSeq 3000 flowcell with 50 cycles single end sequencing by pooling the samples based on molarity aiming for 30 mio ...
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bioRxiv - Cancer Biology 2019Quote: ... The RCC1 domain was deleted using the Quickchange® II XL Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Stratagene) using the primer sequences ...
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bioRxiv - Biophysics 2019Quote: ... The γS N14D and γS N76D were generated via site-directed mutagenesis using QuikChangeXL Kit (Agilent Technologies, Santa Clara, CA). Escherichia coli cell stocks containing the plasmid of interest were grown at 37 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... were dissociated and 106 cells were used to extract total RNA following manufacturer’s instructions of the Absolutely RNA Miniprep Kit (#400800, Agilent Technologies). cDNA was synthesised with 200 ng-1 μg of total RNA using M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: Constructs for the ScSth1p bromodomain mutants (Ser1276Glu, His1272Ala, Trp1338Gln, or Tyr1290Ala/Asn1333Ala) were generated using QuikChange site-directed mutagenesis kit (Stratagene). Mutant bromodomain expression and purification were carried out as for the wild-type protein ...
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bioRxiv - Molecular Biology 2019Quote: ... m6A-mut CIRBP reporter was generated by mutating the essential C in the m6A site synonymously to T using two rounds of site-directed mutagenesis with the QuikChange Lightning kit (Agilent).
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bioRxiv - Genomics 2019Quote: ... Library preparation and target enrichment was carried out using the SureSelect XT Mouse All Exon Kit (Agilent cat.# 5190-4641) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA was extracted at 24 h and 120 h using mirVana Isolation Kit according to manufacturer’s instructions and quantified using TapeStation (Agilent Biotechnologies). Bulk RNA sequencing was performed on biological duplicates using Illumina sequencing (75 million reads) ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA labeling was done either with a two-color Quick Amp Labeling Kit (4×44K arrays) or with a one-color Low Input Quick Amp Kit (8×60K arrays) according the supplier’s recommendations (Agilent Technologies).
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bioRxiv - Microbiology 2019Quote: ... The resulting products were then run with a Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Quantification was performed using the following equation ...
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bioRxiv - Genomics 2019Quote: ... or 100 ng of gDNA was assembled into exome sequencing libraries using the SureSelect Human All Exon V7 Exome kit (Agilent). DNA samples were pooled to achieve an on-bait depth of 100× for the buffy coat gDNA and 300× for the plasma cfDNA.
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bioRxiv - Microbiology 2019Quote: ... Mutation of 1380Asp and 1592Asp to Ala was performed by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent) generating pTOPO-IC-DEAD.
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bioRxiv - Cancer Biology 2019Quote: ... Library preparation was performed using SureSelect Human All Exon Kit v5 (cat# 5990-9857EN, Agilent Technologies, Santa Clara, CA, USA), sequencing was performed on the Illumina Xten platform ...
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bioRxiv - Molecular Biology 2019Quote: ... the membranes were incubated with [α-32P]dCTP radioactively labelled DNA probes (Prime-IT RmT Random Primer Labelling Kit, Stratagene) overnight at 65°C ...
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bioRxiv - Biochemistry 2019Quote: ... and pZeoPTPζ-ΔD2 (the D2 deletion mutant of PTPRZ-B) was generated using pZeoPTPζ as a template with a Quikchange multisite-directed mutagenesis kit (Stratagene).
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bioRxiv - Immunology 2019Quote: ... The quality and quantity of all barcoded DNA libraries were analysed using the High Sensitivity DNA analysis kit (Agilent, UK) on the 2100 Bioanalyzer Instrument and Qubit Fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Single-point mutants in PACRG were generated by PCR mutagenesis using the QuickChange II Site Directed Mutagenesis kit (Agilent Technologies). The pDB070.iodo.5 plasmid containing the modified tRNA and tRNA synthetase for p-iodo-L-phenylalanine incorporation was obtained from Addgene (#99397 ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) and RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... RpoB E310A and RpoB E310K were made by site-directed mutagenesis of the wild type constructs using the Quikchcange II XL kit (Agilent). B ...
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bioRxiv - Biochemistry 2020Quote: ... The pcDNA3.1 Hygro-DEST_VGLL2_Ω_loop mutant was generated by use of the QuikChange Lightning Site-Directed Mutagenesis kit (Stratagene, San Diego, CA), with oligo 5’CCC TTG GAG AGA CTG CAG CTT CTA TCA GGC TCC TGT GCC 3’ (removing amino acid positions 134-149 ...
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bioRxiv - Microbiology 2021Quote: ... Quantity and quality of RNA was assessed on an Agilent 2100 Bioanalyzer using Agilent RNA 6000 Nano kit (Agilent, USA). Extracted RNA was reverse-transcribed to cDNA using SuperScript™ IV First-Strand Synthesis System (Invitrogen™ ...
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bioRxiv - Genomics 2020Quote: ... with a Qubit RNA BR (Broad-Range) or Qubit RNA HS assay kit and with RNA Screen Tape and Sample buffer using TapeStation 4150 (Agilent). RNA with an RNA integrity number (RIN ...
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bioRxiv - Genomics 2020Quote: ... Total RNA integrity and quantity of the samples were assessed using the Agilent 2100 Bioanalyzer with the RNA 6000 Nano LabChip Kit (Agilent) and NanoDrop 1000 Spectrophotometer (Thermo Scientific).
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bioRxiv - Genomics 2021Quote: ... and the quality was assessed using an Agilent 2100 Bioanalyzer with a High Sensitivity DNA analysis kit (Agilent, 5067-4626).