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Citations for Agilent :
4251 - 4300 of 4314 citations for 7 BROMO 2 1 3 BENZOTHIADIAZOLE 4 SULFINIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: DNA from cell lines was isolated as previously described 12 and used to analyze copy number variations by the Array-CGH SurePrint G3 Human CGH Microarray 1×1M (Agilent, Santa Clara, California, USA). Array experiments were performed as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... and with secondary goat anti-rabbit (for polyclonal Ab) or rabbit anti-mouse (for monoclonal Ab) IgG HRP-conjugated Ab (1:2000, Dako A/S, Glostrup, Denmark). Signals were visualized by Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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bioRxiv - Microbiology 2019Quote: ... Desalted tryptic peptides corresponding to 1 μg of protein were loaded for LC-MS/MS analysis with an Agilent 1100 Capillary LC system (Agilent Technologies, San Jose, CA) and run on a Q Exactive mass spectrometer (ThermoFisher Scientific Inc.) ...
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bioRxiv - Biochemistry 2020Quote: ... The TIMS elution voltage was calibrated linearly to obtain reduced ion mobility coefficients (1/K0) using three selected ions (m/z 622, 922, and 1222) of the ESI-L Tuning Mix (Agilent, Santa Clara, CA, USA).
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bioRxiv - Biochemistry 2020Quote: ... Tyrosine fluorescence measurements were carried out in triplicate at 20 °C using a quartz cuvette (Hellma Asia Pte. Ltd. Singapore) at 1 cm path length on a Varian Cary Eclipse (Agilent Technologies Singapore Pte. Ltd) fluorescence spectrophotometer with excitation and emission wavelengths of 275 nm and 305 nm respectively ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 45 minutes at room temperature with the anti-SARS spike glycoprotein antibody 3A2 (rabbit, Abcam ab272420 1:100 diluted in Dako REAL antibody diluent #S2022), which has been validated in previous publications20,41 ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations were generated within the miR-1 seed sequences using the QuikChange Lightning or QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) to disrupt miR-1’s binding and regulatory function (Gregory et al. ...
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bioRxiv - Cell Biology 2022Quote: ... T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM; 103575-100) supplemented with 4mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... Phe322 was substituted for the Tyr322 residue of WT and G307S DNAM-1 by using QuikChange II Site-Directed Mutagenesis Kits (Agilent technologies, Palo Alto, CA) with primers (5′-GAGAGGATATTTTTGTCAACTATCCAACC-3′ and 5′-GGTTGGATAGTTGACAAAAATATCCTCTC-3′) ...
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bioRxiv - Genomics 2019Quote: ... We also ensured the quality of library pools by running 1 µL on a Bioanalyzer High Sensitivity chip (Agilent Technologies, Santa Clara, CA, USA). We combined the iTru and iNext E ...
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bioRxiv - Pathology 2020Quote: ... Abcam, Cambridge, MA), CD68 (LV n=25, IVS n= 26, RV n=22) for macrophages (1:800, clone KP1, Dako/Agilent, Santa Clara, CA), and CD163 (LV n=26 ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblot analysis. Membranes were incubated with primary antibodies (Suppl. Table 1) overnight and probed with respective secondary antibodies (Dako-Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... 1 µl of the sample was injected into an Agilent 7890B equipped with an Agilent 7000D mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). The injector was set to 170°C and heated to 350°C with 200°C/min and hold for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Biochemistry 2021Quote: ... Antigen retrieval using a standard pH 6 sodium citrate buffer (BioGenex) was performed and sections were stained with Monoclonal Mouse Anti-Human CD45 (Dako, M0701, dilution 1:200) using the M.O.M ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with 50 µl/well of HRP-conjugated rabbit anti-mouse total IgG (1:1,000 in ELISA dilution buffer; P0260, Dako Agilent, Santa Clara, CA, USA), goat-anti-mouse IgG1 (1:8,000 in ELISA dilution buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and fluorescence intensity was measured at 340/440 nm over 1 hr at 37 °C using a BioTek Synergy HTX (Agilent, Santa Clara, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... The fluorogenic substrate was added to each well of a 96-well plate and the fluorescence intensity was measured at 340/440 nm over a 1 hr period at 37 °C using a Bio-Tek Synergy HTX (Agilent, Santa Clara, CA, USA) plate reader.
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bioRxiv - Plant Biology 2023Quote: ... Peptides were desalted using C18 solid phase extraction columns (Bond Elut SPEC C18, 96 round-well plate, 15 mg, 1 mL, Agilent Technologies, Santa Clara, USA) and a water-jet (vacuum ...
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bioRxiv - Bioengineering 2024Quote: ... GBM cells within hydrogels were seeded at a density of 1 × 105 cells/well in 96-well spheroid microplates (Agilent, catalog number 102959-100). In the Mito Stress Test ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections on slides were baked at 60°C for 1 h and then deparaffinized and retrieved on Dako Omnis fully automated IHC platform (Agilent, Santa Clara, CA, USA) using EnVision FLEX target retrieval solution ...
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bioRxiv - Microbiology 2023Quote: ... tissues were incubated with proteinase K diluted 1:10 in 1X TBS (Fisher Scientific, Cat. #BP24711; and PNA ISH kit, Agilent Dako, Cat. #K5201) for 10 min at RT in a humidity chamber ...
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bioRxiv - Neuroscience 2023Quote: ... samples were treated with Bond Elut Plexa Solid Phase Extraction (SPE) cartridges (30 mg, 1 mL, Part No. 12109301, Agilent Technologies, Inc., CA, USA) and hormones were separated on an Infinity Lab Poroshell HPH-C8 column (2.1 × 50 mm ...
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bioRxiv - Immunology 2023Quote: One microliter of resuspended Captiva flow-through fraction or 1 uL of standard were injected on LC-MS systems consisting of UHPLC (Agilent, 1200 Infinity II Biocompatible) coupled to triple quadrupole MS (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... Aliquots of 1 μL were injected into the column (HP5-MS, 30 m, 250 μm ID, 2.5-μm film, Agilent Technologies, Palo Alto, CA, USA) using a pulsed split-less mode and He as carrier gas at a flow rate of 1 mL min-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF10A shCtrl and shEIF3E cell lines were transfected in 100 mm tissue culture plates with 5 μg of DNA at a ratio of 1:50 of a T7-tagged PRP4K expression vector (16) or pBluescript SK-(Stratagene/Agilent Technologies, Santa Clara, CA, USA) using the Neon Transfection System (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2 or with negative isotype control mouse IgG2a (cat. X0943 - Agilent Technologies, Santa Clara, CA, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA integrity and quality were assessed by means of a 1% agarose electrophoresis gel and in Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Bioengineering 2020Quote: ... a high-power resistor of 1 Ω was placed in series with the coils and voltage drop across the resistor was observed with an oscilloscope (Agilent Technologies, Santa Clara, CA) during PEMF exposure period ...
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bioRxiv - Genomics 2020Quote: ... and assessed for quality using a 1 % (w/v) pulse-field gel electrophoresis and with genomic ScreenTape on the TapeStation 2200 platform (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... A 1 µL sample of the derivatized material was analyzed in splitless mode using a 7200 GC-QTOF system (Agilent Technologies, Santa Clara, CA, USA). A solution of fatty acid methyl esters (C8 to C30 ...
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bioRxiv - Cell Biology 2020Quote: ... and pom1521-200-GFP (pKBB520) fusions were generated by amplifying sGFP from pJK19-1 using PCR with PfuUltra™ HF (Agilent, Inc. Santa Clara, CA) and primers that generated chimeric Pom152-sGFP PCR products for homologous recombination in yeast ...
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bioRxiv - Microbiology 2021Quote: ... The integrity of RNA extracts was evaluated using gel electrophoresis on 1% agarose in 1x TBE and the Agilent 2200 TapeStation System (Agilent Technologies, Santa Clara, CA, USA). Libraries were prepared from three samples per treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblot analysis. Membranes were incubated with primary antibodies (Suppl. Table 1) overnight and probed with respective secondary antibodies (Dako-Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Plant Biology 2021Quote: ... Dried metabolites were methoximated (20 mg/mL methoxyamine in pyridine) and trimethylsilylated (MSTFA: TMSCI, 99:1) and then analyzed by GC-MS (Agilent 5975, GC/single quadrupole MS). GC-MS data were processed by Agilent MSD ChemStation ...
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bioRxiv - Microbiology 2020Quote: ... and D67N/K70R/T215F/K219Q viruses were generated by site-directed mutagenesis on an xxLAI HIV-1 backbone using the QuikChange XL Site-Directed Mutagenesis kit (Agilent Technologies Inc, Santa Clara, CA), according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: ... the cells were washed once with the same buffer and incubated with FITC-conjugated polyclonal swine anti-rabbit immunoglobulin antibody from DAKO (dilution 1:40 in BP) for 1 hr at 4°C on ice with shaking ...
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bioRxiv - Microbiology 2022Quote: ... CFSs were diluted in SDW (ratio 1/20) and injected into a high-performance liquid chromatography (HPLC) system (Agilent 1100 Series 203 HPLC System, Agilent Technologies, Palo Alto, CA, USA), equipped with a diode array and quaternary pump ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Microbiology 2024Quote: ... Twenty-microliter reactions were prepared with 1×Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies, Santa Clara, CA, USA), 1 µg μl-1 bovine serum albumin (New England Biolabs® Inc. ...
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bioRxiv - Microbiology 2023Quote: Headspace CO2 of fungal incubations (1 mL) were measured for CO2 concentration on a Hewlett Packard HP 5890 Series II GC (Agilent Technologies, Inc, Santa Clara, USA) coupled with a Thermal conductivity detector (TCD) ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at room temperature for 1 hour and analyzed using gas chromatography-mass spectrometry (6890N GC, coupled to 5873N Mass Selective Detector; Agilent Technologies, Santa Clara, CA, USA). Statistical significance was assessed by mixed linear model with pBMI ...
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bioRxiv - Molecular Biology 2023Quote: Optimal temperature was measured using the enzyme activity assay described above in 1 mL cuvettes using a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Santa Clara, CA, USA) at 22 °C and temperatures from 30 °C to 50 °C with 5 °C increments using a circulating water bath ...
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bioRxiv - Neuroscience 2023Quote: ... IDH 1 (R132H) (QM002; quartett GmbH, Potsdam, Germany), ATRX (BSB-108; Bio SB, Goleta, CA, USA) and Ki67 (MIB1; Agilent Technologies, Santa Clara, CA, USA) were routinely performed according to standard protocols.
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bioRxiv - Cancer Biology 2023Quote: ... IHC was performed on an automated Ventana Discovery Ultra staining system using primary antibodies recognizing Ki-67 (M7240, DAKO, mouse monoclonal, clone MIB1, 1:50) for 30min ...
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bioRxiv - Microbiology 2023Quote: K65R and M184V mutations in RT were generated by site-directed mutagenesis on an HXB2 or LAI HIV-1 backbone using the QuikChange XL site-directed mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... Desorption was performed at 250°C for 1’ (splitless mode) in the injection port of a 6890 N gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) coupled to PEGASUS 4D mass spectrometer (LECO Corporation) ...