Labshake search
Citations for Agilent :
4101 - 4150 of 4255 citations for 6H 1 2 Thiazine 6 ethoxy 5 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... A capillary column was the stationary phase (30 m x 0.53 mm x 1 µm film thickness; Agilent Technologies, Santa Clara, CA), and helium was used as the mobile phase (at a flow rate of 8.5 mL/min) ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody detection was followed by 45 min of incubation with goat anti-mouse-biotin (DAKO, E0433, 1:200 in TNB blocking buffer) and subsequent incubation with Streptavidin-HRP Conjugate (PerkinElmer ...
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bioRxiv - Neuroscience 2019Quote: ... Endogenous peroxides were quenched with 1 % hydrogen peroxide in 10 % methanol for 10 minutes followed by blocking in antibody diluent (Dako /S 202230) for 10 minutes ...
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bioRxiv - Physiology 2019Quote: ... for 15 minutes and then incubated overnight at 4°C with primary antibodies diluted in DAKO antibody diluent: anti-Insulin (DAKO, 1:1000), anti-BRD4 (Bethyl laboratories ...
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bioRxiv - Synthetic Biology 2020Quote: ... and was allowed to pass through the packed catalyst bed at space velocities in the range of 1-45 s−1 by helium as the diluent gas.44 The injection was immediately followed by separation using HP-PLOTQ column (Agilent, 19091P-QO4), and compounds in the effluent stream were identified using online Agilent 5975C Triple-Axis MS detector ...
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bioRxiv - Microbiology 2020Quote: ... Cells were next washed three times with PBS and incubated with a secondary fluorescein isothiocynate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200) in 1% BSA for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with Bond Wash buffer 3 x 30s and then incubated with either mouse anti-CD68 (M0814, 1:100, Dako, Carpenteria, CA), mouse anti-βIII-Tubulin (G7121 ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies are diluted in 1x blocking solution and incubated with antibodies against insulin (1:2000, guineapig, Dako, cat. no. A-0546), glucagon (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... 190 to 601 nm (steps: 1 nm) and a Q-TOF (tandem mass spectrometry, quadrupole and Time of flight detector combined) (Agilent, Waldbronn, Germany). Electrospray ionization was performed in positive mode (ESI+ ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Immunology 2021Quote: ... and a 1:10 dilution of the resulting cDNA was run on a Bioanalyzer High Sensitivity DNA chip (Agilent Technologies #5067-4626) to assess cDNA quality and yield ...
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bioRxiv - Immunology 2021Quote: ... post-amplification and a 1:10 dilution of the resulting HTO libraries were run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess HTO quality and yield ...
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bioRxiv - Cell Biology 2021Quote: ... and deparaffinized prior to antigen retrieval in 0.1M sodium citrate pH6 and staining with guinea pig anti-insulin antibody (Dako A0564, 1:250) overnight at 4 degrees in the dark and visualized with Anti-Guinea Pig biotin conjugated antibody (Vectastain BA7000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... The phPAI-1 library was generated by error prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) with primers that maintain the AscI and NotI restriction sites (SI Table 1) ...
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bioRxiv - Biophysics 2022Quote: Fluorescence titrations were carried out at 25 °C using 1 × 0.3 cm path-length cuvettes in a Cary Eclipse spectrofluorimeter (Agilent Technologies, Waldbronn, Germany). Experiments were carried out in 20 mM K-phosphate ...
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bioRxiv - Immunology 2022Quote: ... post-amplification and a 1:10 dilution of the resulting HTO libraries were run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess HTO quality and yield ...
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bioRxiv - Molecular Biology 2022Quote: ... Adapter dilution (1:4) and PCR amplification (26 cycles) were optimized in a pilot experiment using Bioanalyzer DNA1000 (Agilent, Sant Clara, USA) chips as a read out of library size and quantity ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-mouse IgG (DAKO, cat. P0447) or HRP-labelled polyclonal goat anti-rabbit IgG (DAKO ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-rabbit IgG (DAKO, cat. P0448) or HRP-labelled polyclonal goat anti-mouse IgG (DAKO ...
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bioRxiv - Cell Biology 2024Quote: Protein expression was initiated by transforming ∼80 ng of each DNA plasmid into 50 µL of XL-1 Chemically Supercompetent Cells (Agilent Technologies, 200236) at 42 °C for 30 seconds followed by 5 minutes of incubation on ice ...
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bioRxiv - Immunology 2024Quote: ... The final PCR products were purified by AMPure XP beads at a 0.8:1 bead to sample ratio and quality controlled by TapeStation (D1000, Agilent, cat# 5067-5584). The samples were pooled at equimolar concentrations and further purified by gel extraction and AMPure XP bead clean up.
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bioRxiv - Plant Biology 2024Quote: ... 1:500 cDNA dilutions were analyzed using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies, Santa Clara, CA, USA), required primer pairs (Table S2) ...
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bioRxiv - Cancer Biology 2024Quote: ... at 1:400 for two hours and then incubated for 30 minutes with Rabbit Envision HRP System reagent (Agilent, Santa Clara, CA). For vimentin staining ...
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bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.
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bioRxiv - Microbiology 2023Quote: ... Fluorescence was measured at 50°C over 1 h at excitation 360 nm emission 460 nm on a BioTek Synergy HTX Multimode Reader (BioTek, Agilent, Tx, USA). The reaction rate was calculated as the maximum velocity over 12 sequential readings ...
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bioRxiv - Synthetic Biology 2023Quote: ... composed of 1 ng of the cDNA library quantified using TapeStation with High Sensitivity D5000 ScreenTape (Agilent #5067-559 and #5067-5593), 0.7 µL each of 20 µM forward primer P5-TSO_Hybrid (45 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were loaded onto a ZORBAX SB-C18 column (150 x 1 mm, 3.5 μm, flow of 90 μl/min; Agilent, Les Ulis, France) heated at 40°C ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Biochemistry 2022Quote: ... The metabolite-containing supernatant (195 µL) was transferred into pre-cut Bond® Elut PH (100 mg, 1 ml) SPE cartridges (Agilent, 12102005) placed in 1.7 mL microcentrifuge tubes and then centrifuged at ∼3000 g for 3 min ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal rabbit anti-mouse secondary antibody IgG/HRP (P0260; Agilent Technologies/Dako) diluted 1:2000 in 3% BSA.
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bioRxiv - Developmental Biology 2023Quote: Whole-mount immunostaining of differentiated skeletal muscle cells was performed using the monoclonal hybridoma 12/101 primary antibody [38] (1/200 dilution; DSHB #AB-531892) and the EnVision+ Mouse HRP kit (Agilent Technologies, K4007) according to the manufacturer’s recommendations.
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bioRxiv - Microbiology 2023Quote: ... fitted with a flame photometric detector (FPD) and a J&W DB-1 column (30 m x 0.32 mm Ø; Agilent Technologies, USA). The oven temperature was 180°C ...
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bioRxiv - Physiology 2023Quote: ... For β-cell proliferation analysis in P1 pups every other section was labeled with guinea pig anti-insulin (1:200; DAKO A0564) and mouse anti- BrdU (1:500 Sigma B2531 ...
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Mass spectrometric analysis and biostimulatory effects of boar seminal gel, saliva and semen in pigsbioRxiv - Animal Behavior and Cognition 2023Quote: ... An aliquot of 1 μL was injected into GC-MS (GC 9000 and MS of G7077B, Agilent Technologies, Palo Alto, CA, USA) for further analysis ...
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bioRxiv - Biophysics 2023Quote: ... the following HIV-1 IN mutants were prepared using the QuikChange II XL site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA): E138K ...
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bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.
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bioRxiv - Synthetic Biology 2023Quote: ... 100 μL of the ethyl acetate phase was transferred into a GC vial with insert and 1 μL was analyzed using GC 8890 (Agilent Technologies, USA) equipped with a flame ionization detector (FID ...
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bioRxiv - Microbiology 2023Quote: ... Gas samples from the headspaces (250 μl) were collected using a 1 mL Hamilton’s SampleLock™ syringe and manually injected into a Gas Chromatograph (GC) (Agilent 7820A, AgilentTechnologies). H2 and O2 were separated using a packed column (60/80 Molecular Sieve 5A ...
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bioRxiv - Developmental Biology 2023Quote: ... The quality and quantity of the amplified sequencing libraries were validated using a Fragment Analyzer SS NGS Fragment Kit (1–6000 bp) (Agilent, Waldbronn, Germany). The equimolar pool of samples was spiked into a NovaSeq6000 run targeting 20M reads per sample on a S1 FlowCell (Novaseq S1 Reagent Kit ...
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bioRxiv - Neuroscience 2023Quote: ... followed by site-directed mutagenesis at Cys322 site to Serine using QuikChange Site-directed mutagenesis kit (Agilent, Santa Clara, CA; Fig. 1). 4RCF construct (four microtubule-binding repeats and cysteine-free construct containing C291S and C322S mutations ...
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bioRxiv - Genomics 2023Quote: ... The average library size was obtained via analysis of 1 μL of the library on the Agilent TapeStation 2200 (Agilent, Cat # G2964AA) using D1000 screen tape (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of phosphorylated (pS396/404) tau were determined by a MSD sandwich ELISA using a polyclonal total tau antibody (DAKO, AA002402-1) as a capture antibody and a monoclonal pS396/404-tau antibody (PHF1 ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 1 × 105 cells per well were seeded on a 24-well Xfe96 Cell culture Microplate (Agilent, Cat# 100777-004) coated with poly-L-lysine (Millipore Sigma ...
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bioRxiv - Pathology 2024Quote: ... One hour of shaking incubation at 30℃ resulted in the extraction of 1 mL of gas for ethylene measurement using gas chromatography (Agilent 7820A, USA).
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 90 minutes in a biotin conjugated secondary antibody (a goat anti-rabbit, a goat anti-mouse or a swine anti-rabbit as appropriate; Dako; diluted 1:500) and for 1h in a peroxidase conjugated extravidin (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Purified cDNA was subjected to quality control using 1 µl of cDNA on an Agilent 2100 BioAnalyser (Agilent Technologies, Santa Clara, CA, USA) using the Agilent High Sensitivity DNA kit ...
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bioRxiv - Molecular Biology 2019Quote: ... concentration and purity were evaluated on an Agilent RNA6000 Nano Chip in Reagent Port 1 of the Bioanalyzer Agilent 2100 (Agilent Technologies, CA, USA). Sequencing of the libraries was done on the Illumina HiSeq 4000 platform by BGI Co. ...
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bioRxiv - Bioengineering 2019Quote: ... The following primary antibodies were used for immunocytochemistry experiments: rabbit anti-glial fibrillary acidic protein (GFAP) (DAKO, 1:100, Z-0334, Lot #2002332), mouse anti-vascular endothelial (VE)-cadherin (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako, Santa Clara, CA, USA Z0311, 1:400). Following washing ...