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Citations for Agilent :
4051 - 4100 of 4217 citations for Mouse Anti Dengue Virus Serotype 2 NS1 Antibody CM435 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed once with DMEM Assay Medium (XF DMEM [Agilent] supplemented with XF glucose [10 mM, Agilent], XF glutamine [2 mM, Agilent] and XF pyruvate [1 mM, Agilent]), then left in DMEM Assay Medium and placed in a non-CO2 incubator for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... VWF in supernatant was detected by Western blot with a rabbit polyclonal antibody against VWF ((Dako Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: ... VWF in supernatant was detected by Western blot with a rabbit polyclonal antibody against VWF ((Dako Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Immunology 2022Quote: ... tissues fixed for 24-30 hours in 10% neutral-buffered formalin (NBF; 3.7% formaldehyde) as previously described (Wiarda et al., 2020) with polyclonal rabbit α-human CD3ε antibody (Dako A0452) and polyclonal goat α-rabbit horseradish peroxidase (HRP)-conjugated antibody (Dako K4003) ...
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bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
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bioRxiv - Developmental Biology 2019Quote: ... Brain sections that were stained with antibodies that required antigen retrieval were incubated in Dako 1X target retrieval solution (Agilent Dako) or sodium acetate buffer ...
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bioRxiv - Immunology 2019Quote: ... Immuno-affinity purification through Protein-A Sepharose beads covalently bound to W6-32 antibodies was performed in a format of 96-well single-use micro plate with 10 µm polypropylene membranes (SeaHorse Bioscience). The plates were then washed 4 times with 2mL 150 mM NaCl and 20 mM Tris HCl (buffer A) ...
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bioRxiv - Neuroscience 2019Quote: Immunohistochemistry was performed on colon sections as described earlier on brain sections58 using additional antibodies (α-Syn: ab27766, Abcam or L001, Enzo; NeuN: MAB377, Merck; MAP2: ab70218 Abeam; TH: 657012, Merck; GFAP: Z0334, Dako).
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bioRxiv - Neuroscience 2021Quote: ... and the membrane was blocked with 5% milk in tris-buffered saline with Tween 20 (TBST) before immunodetection with the following primary antibodies: tau (1:1000, DAKO, Denmark), pTau-S396 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunostaining was performed as described previously(45) with the following antibodies: guinea pig α-insulin (1:2000, Millipore/1:800, DAKO), mouse α-glucagon (1:2000 ...
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bioRxiv - Cancer Biology 2020Quote: ... paraffin-embedded human sections were immunolabeled for CD68 and CD163 (details of antibodies are available in Supplementary Table S3) and counterstaining with Mayer’s hematoxylin (cat. #S3309; Dako, Jena, Germany). Immunohistochemical images were analyzed in collaboration with the anatomical pathology laboratory of Hospital North using Calopix software (TRIBVN ...
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bioRxiv - Neuroscience 2022Quote: ... 3x 5 minutes) and incubated in the secondary antibody-horseradish peroxidase (HRP) complex as part of REAL EnVision detection system (Dako #K5007) for 1h at RT ...
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bioRxiv - Genetics 2020Quote: ... serial sections were stained singly with antibodies against BCL6 and CD8 and a DAB DAB visualization kit (Envision Double Stain system, Dako; USA) for bright field microscopy ...
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bioRxiv - Neuroscience 2019Quote: ... The slides were incubated overnight in primary antibody (ATRX: 1:200, H-300 Santa Cruz Biotechnology Inc; GFAP: 1:200, Agilent Technologies ...
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bioRxiv - Physiology 2021Quote: ... sections were immunostained with alpha-smooth muscle actin (α-SMA) antibody (Clone 1A4, DAKO, M0851, Agilent Technologies, Santa Clara, CA, USA). Slides containing gastrocnemius muscle sections were deparaffinized in xylene and rehydrated using a graded ethanol series ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA ...
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bioRxiv - Immunology 2020Quote: ... The primary antibodies used for immunohistochemistry were: antihuman HLA-DR (referred to from here as MHC-II) (Dako M0746; 1:40); anti-human CD3 (Dako A0452 ...
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bioRxiv - Developmental Biology 2020Quote: ... The sections were washed in TBS and Tween-20 and stained for 1 hour with HRP-labelled secondary antibody (Dako K4003). The staining was developed with diaminobenzidine (DAB ...
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bioRxiv - Pathology 2024Quote: ... sections were incubated overnight at 4 ⁰C with a cocktail of the primary antibodies αSyn (clone KM51 1:500, Monosan Xtra, The Netherlands) and glial fibrillary acidic protein (GFAP, Z0334, 1:4000, DAKO, Denmark). After rinsing in PBS ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... the sections were incubated with a secondary antibody labeled with a polymer as part of the Envision+ system containing horseradish peroxidase (Dako Cytomation). The following primary antibodies and dilutions were used to stain mouse tissues ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies). Lager scale transient transfections were purified on the Akta FPLC system using 1mL HiTrap MabSelect PrismA™ affinity chromatography resin (Cytiva) ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were incubated in the following primary antibodies for 3 h at 24L: gastrin (1:1000, Cat #A0568, A0568, AB_2757270, Dako Agilent Technologies), Chromogranin A (1:100 ...
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bioRxiv - Bioengineering 2023Quote: ... Specific site directed mutations on the S309 and CMAB0 antibody sequence was done using the Quick-change site directed mutagenesis kit II (Agilent technologies).
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with Alexa Fluor®-conjugated secondary antibodies for 1 hour at room temperature and mounted on slides using DAPI-containing mounting media (Dako). Images were acquired using a Zeiss LSM 800 series confocal microscope at the Yale CCMI facility ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibody against pS65-Ub (in- house, 1:250) [20] that was diluted in Dako Antibody Diluent with Background Reducing Components (Agilent, S3022) for 45 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Permeabilised pancreatic slices were blotted with ready-diluted anti-guinea pig insulin (Agilent Technologies, USA) and anti-mouse glucagon (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... followed by polyclonal Rabbit anti-human Glucagon (cat. A0565, Agilent Technologies, Santa Clara, CA, USA) diluted 1:500 in PBS 1X supplemented with 3% BSA ...
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bioRxiv - Genetics 2022Quote: ... Immunohistochemistry was also conducted using anti-neurofilament 200 (Ready to use DAKO clone2F1 - reference: IS607) and anti-synaptophysin Ready to use clone DAKO-SYNAPTO - reference ...
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bioRxiv - Physiology 2022Quote: ... fetal capillary endothelium and placental trophoblasts were distinguished using anti-vimentin (Dako Vim3B4: 1:100) and anti-pan cytokeratin (Sigma C2562 ...
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bioRxiv - Physiology 2020Quote: ... blocked in 20% fetal calf serum (FCS) and incubated with anti-insulin (1/10, Dako) in Antibody Diluent for 2h ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and subsequently polyclonal goat anti-rabbit Immunoglobulins conjugated with HRP (Agilent Dako, Santa Clara, CA), following the manufacturer instructions.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and subsequently polyclonal goat anti-rabbit Immunoglobulins conjugated with HRP (Agilent Dako, Santa Clara, CA), following the manufacturer instructions.
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bioRxiv - Immunology 2019Quote: ... 4°C overnight followed by HRP conjugated goat-anti-rabbit IgG (#P0448, Dako (1:1000). α-Tubulin was used as a housekeeping control (#sc-32293 ...
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bioRxiv - Immunology 2021Quote: ... beads were incubated with 5 μg/ml FITC-conjugated rabbit anti-human C1q (Dako, F0254).
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bioRxiv - Cell Biology 2022Quote: ... Goat Anti-Rabbit Immunoglobulins/HRP (dilution 1:100,000, #P0448, Dako Denmark A/S, Glostrup, Denmark) for 1h in RT ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated in methanol-validated polyclonal rabbit anti-GFAP IgG (Agilent Cat# Z0334, RRID:AB_10013382) at a dilution of 1:1000 in PBS/Triton-X/donkey serum/DMSO solution for 4 days at 37°C ...
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bioRxiv - Physiology 2022Quote: ... Slides were incubated overnight at 4°C in rabbit anti-GFAP (Agilent Cat# Z0334, RRID:AB_10013382) at a dilution of 1:2000 in antibody diluent (Agilent Cat# S0809) ...
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bioRxiv - Neuroscience 2022Quote: ... transferred onto glass slides and mounted for visualization with anti-fading mounting medium (DAKO Fluoromount).
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bioRxiv - Neuroscience 2022Quote: ... transferred and mounted onto glass slides with anti-fading mounting medium (DAKO; Agilent, Cat# S3023) for visualization ...
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bioRxiv - Neuroscience 2022Quote: ... transferred and mounted onto glass slides with anti-fading mounting medium (DAKO; Agilent, Cat# S3023) for visualization ...
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bioRxiv - Neuroscience 2024Quote: ... transferred onto glass slides and mounted for visualization with anti-fading mounting medium (DAKO Fluoromount).
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bioRxiv - Microbiology 2024Quote: ... The western blot analysis was performed on PVDF membrane using rabbit anti-IgG-HRP (Dako), mouse anti-Albumin (Tebu-bio) ...
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bioRxiv - Immunology 2024Quote: ... CBMCs were activated with polyclonal rabbit anti-human IgE Ab (5 µg/mL, Dako, #A0094).
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed in TBST (3 × 15 mins at RT) and then incubated with corresponding peroxidase-conjugated secondary antibodies (Dako, P0447, P0448) for 1h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... 150,000 cells (activated with anti-CD3 and anti-CD28 antibodies 48 hours prior) were plated into individual wells of a poly-D-lysine coated seahorse cell culture microplate (Agilent; 101085-004). T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM ...
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bioRxiv - Immunology 2020Quote: ... followed by the incubation with horseradish peroxidase-labeled secondary antibody (1:10,000) for 1 h at room temperature (DAKO EnVision™+ System; Agilent Technologies (UK). Diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2019Quote: ... Endogenous peroxides were quenched with 1 % hydrogen peroxide in 10 % methanol for 10 minutes followed by blocking in antibody diluent (Dako /S 202230) for 10 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... The slides were washed in PBS (0.05 M, pH 7.2) 3x and incubated with the secondary antibody (DAKO Detection System - K0690, USA). The slides were washed again in PBS and then incubated with the streptavidin-peroxidase (DAKO Detection System - K0690 ...