Labshake search
Citations for Agilent :
4051 - 4100 of 4135 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: 384 well qPCR reaction plates for the multiplexed activation of endogenous genes (Figure 5 and 6) were set up using the Brilliant II SYBR master mix (Agilent cat #600828) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2020Quote: ... 5890 Series II gas chromatograph equipped with an HP-5 column (30 m×0.32 mm ID× 0.25 mm) (Agilent, Palo Alto, CA, USA). The oven temperature was held at 40 °C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... column protected with an ZORBAX RRHD Eclipse Plus C18 5 × 2.1 mm ID (1.8 μm) guard column (Agilent Technologies, Foster City, CA). The mobile phase consisted of water and methanol (both added 0.1% formic acid ...
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bioRxiv - Genomics 2020Quote: Dried peptides were separated by high pH reverse phased liquid chromatography on an Agilent 1100 HPLC system using a Zorbax Eclipse column (2.1 × 150 mm, 5 um) (Agilent Technologies, Palo Alto). Peptides were eluted with a linear gradient of 20 mM ammonium formate pH 10 ...
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bioRxiv - Molecular Biology 2023Quote: A fluorescent image of rehydrated brain tissue with a thickness of 275 μm stained with Amy tracker and placed on glass microscopic slides was acquired using the manual mode in a Cytation 5 multimode reader (Agilent Technologies, USA). Montage images were collected at 4x and 20x magnification ...
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bioRxiv - Molecular Biology 2023Quote: ... column protected with an ZORBAX RRHD Eclipse Plus C18 5 x 2.1 mm ID (1.8 µm) guard column (Agilent Technologies, Foster City, CA). The mobile phase consisted of water and methanol (both added 0.1% formic acid ...
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bioRxiv - Neuroscience 2023Quote: ... Pools were diluted to 5 ng/µL and quality and size are assessed using Agilent DNA High Sensitivity chip (Agilent Technologies, Inc.) on the Bioanalyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... The dried labelled peptide plexed sample was then resuspended in 400uL of 1 M TEAB and fractionated using an Agilent 1100 HPLC system and high pH reversed phase chromatography on a Zorbax Eclipse XDB-C18 column (4.6 x 150mm, 5-micron) from Agilent (Santa Clara, CA). Sixty fractions were collected at 0.5 mL/min over 105 min using a gradient of 0-5% solvent B in 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... PSC27 was seeded at a density of 5 × 104 cells/ well in the XF24 cell culture microplate (Agilent Technologies, 04721 and Q01321) at a 37[5% CO2 incubator for overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Drug was then washed out and 1x105 cells plated in 5 replicate wells for immediate analysis using the Mito Stress Test Kit (Agilent, 103015-100) according to the manufacturer’s instructions and using the following drug concentrations ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed in PBS 5 x 5 mins, then mounted with a cleaned glass coverslip (#1.5, 24 mm x 60 mm) in mounting media (Dako; Pathology Products, S3023).
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bioRxiv - Immunology 2023Quote: ... Bacteria were fixed with 0.5% formaldehyde at RT for 30 min and then wells were washed twice with a washer dispenser (BioTek EL406, Agilent Technologies, US) with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... fibroblasts were seeded at 45 × 103 cells per well (5-10 replicates per cell line) in fibroblast growth media in a Seahorse XFe24 Microplate (Agilent, 100777-004) and incubated for 24 hours to allow cells to adhere ...
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bioRxiv - Bioengineering 2023Quote: ... were obtained by high pressure liquid chromatography on an Agilent Bio SEC-5 500 Å (for KWOCA assemblies) or 1000 Å column (for I32-10) (Agilent) at a flow rate of 0.35 mL/min by injection of 10 μL of purified eluate using a mobile phase of Tris-buffered saline (50 mM Tris pH 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were washed thrice for 5 min in TBS-T and incubated with Horseradish peroxidase secondary antibodies (DAKO, Santa Clara, USA) for 1 hr at RT ...
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bioRxiv - Plant Biology 2023Quote: ... samples were separated on an Eclipse XDB-C18 analytical column (250 mm x 4.6 mm, 5 μm, Agilent, Santa Clara, CA, USA). The mobile phase solvents were composed of 1% acetic acid in water (solvent A ...
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bioRxiv - Cancer Biology 2023Quote: ... Blocking with endogenous peroxidase was performed with Protein Block (Novocastra Laboratories) for 5 min and subsequent steps were performed in DAKO autostainer link 48 (Agilent Technologies, Inc.). Slides were counterstained in hematoxylin-eosin ...
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bioRxiv - Neuroscience 2024Quote: ... Pools were diluted to 5 ng/μL and quality and size are assessed using Agilent DNA High Sensitivity chip (Agilent Technologies, Inc.) on the Bioanalyzer ...
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bioRxiv - Plant Biology 2024Quote: ... CMV genomic RNAs (strain Fny) were synthesized with 5’ monomethylated cap analog by T7 RNA Polymerase (Agilent Technologies, Santa Clara, CA, #600123) from PCR product templates (see Supplementary Table S1 for primer sequences) ...
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bioRxiv - Neuroscience 2024Quote: ... The gas chromatograph was equipped with a 5 m guard column + 30 m (I.D. 250 μm, film 0.25 μm) DB-35MS capillary column (Agilent J&W GC Column). Helium was used as the carrier gas with a constant flow rate of 1.2 mL/min.
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bioRxiv - Biochemistry 2024Quote: ... containing the assembled ribosomes from 25 µL mixtures and 5 nM DNA template encoding sfGFP were incubated at 37 °C in Mx3005P (Agilent Technologies, USA), and sfGFP fluorescence was monitored during incubation.
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bioRxiv - Biochemistry 2024Quote: ... containing the assembled ribosomes from 25 µL mixtures and 5 nM DNA template encoding sfGFP were incubated at 37 °C in Mx3005P (Agilent Technologies, USA), and sfGFP fluorescence was monitored during incubation.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (NeuN anti-mouse EMD Millipore MAB377, GFAP anti-mouse, PSD-95 anti-rabbit abcam ab18258, S100B anti-rabbit Dako Z0311, SYT-1 anti-goat LSBio LS-B5899), secondary antibodies (488 anti-mouse ...
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bioRxiv - Microbiology 2020Quote: ... Sample quality control was assessed using a Qubit 4 Fluorometer (using Qubit™ dsDNA BR assay Kit) and Agilent 2200 TapeStation (using Agilent D5000 ScreenTape assay kit). Library construction and sequencing was undertaken at Novogene (Beijing ...
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bioRxiv - Cancer Biology 2020Quote: ... blocked in 0.5% hydrogen peroxide for 5 minutes at room temperature and then blocked with 20% normal rabbit or goat serum (DAKO, Agilent, Cheadle, Cheshire, UK) for 20 min and incubated with the primary antibody overnight at +4°C ...
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bioRxiv - Biophysics 2021Quote: ... Mutations of key contact points between docked ligands and the human 5-HT5AR binding pocket were made via site-directed mutagenesis as directed (Stratagene, La Jolla, CA). Quickchange II mutagenic primer sets were the following ...
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bioRxiv - Microbiology 2021Quote: The determination conditions of bacillomycin D and fengycin were established by injecting 10 μL samples into an HPLC column (Eclipse XDB-C18, 5 μm; Agilent, Santa Clara, CA). The temperature was maintained at 30 °C during the experiment ...
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bioRxiv - Cell Biology 2021Quote: ... was then mutated to either R to mimic deacetylated c-Myc (K323R) or Q to mimic acetylated c-Myc (K323Q) using QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200522-5) against pPHAGE-EF1α-HA-Puro-c-Myc ...
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bioRxiv - Biophysics 2022Quote: ... 1 and 3.5 μm diameter fibers were manufactured from 2 and 5 wt% of high molecular weight polystyrene (MW: 15,000,000 g/mol, Agilent Technologies, Santa Clara, CA, USA) and equally spaced at approximately 60 μm ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples analyzed by mass spectrometry were resolved using a Poroshell StableBond 300 C8 (2.1 x 75 mm, 5 μm, Agilent Technologies part #660750-906) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Biochemistry 2022Quote: ... The pcDNA3.1 Flag-mRBPJ A284V CRr and the pcDNA3.1 Flag-mRBPJ F261A/A284V CRr were generated via site directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies 200521-5) accordingly to manufacturer’s instructions with the oligos listed in Table S6 and using the pcDNA3.1 Flag-mRBPJ WT CRr and the pcDNA3.1 Flag-mRBPJ A284V CRr as templates ...
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bioRxiv - Microbiology 2020Quote: ... The derivatized samples were analyzed on an Agilent GC-MS (GC model 7890A, MS Model 5975C) equipped with a (5% phenyl)-methylpolysiloxane capillary column (Agilent model HP-5MS). The injection port temperature was held at 280 °C and the oven temperature program was held at 80 °C for 1 min ...
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bioRxiv - Biochemistry 2021Quote: ... Analyses were performed on an Agilent Infinity 1260 HPLC system equipped with a 300 × 7.5 mm PLgel SE-HPLC column (particle size 5 μm, pore size 100 Å) (Agilent Technologies, Waldbronn, Germany). Dichloromethane was used as eluent at a flow rate of 1.00 ml/min ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli was carried out with a ZORBAX SB-Aq StableBond Analytical column (5 μm, 4.6 × 250 mm, Agilent Teologies, Santa Clara, CA, USA). A linear gradient of acetonitrile and water with 0.1% trifluoroacetic acid was used for development of the column at a flow rate of 0.8 mL/min ...
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bioRxiv - Genetics 2021Quote: ... Samples were frozen at -80 C until volatiles were separated using a DB-5 column on a Agilent 6890N gas chromatograph attached to an Agilent 5975 mass spectrometer (Agilent, Palo Alto, CA). Volatile quantitation was preformed using ChemStation software (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... (N-acetyl)paenilamicin-containing fractions were concentrated and purified subsequently by using a Grace HPLC column (GROM-Sil 120 ODS-5-ST, 10 µm, 250 × 20 mm) coupled to an Agilent 1100 HPLC system (Agilent Technologies, Waldbronn, Germany) with a MWD UV detector ...
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bioRxiv - Immunology 2021Quote: Final V(D)J and 5’ GEX libraries were run on a Bioanalyzer 2100 with a High Sensitivity DNA kit (Agilent, Santa Clara, CA) to assess library size and concentration ...
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bioRxiv - Bioengineering 2024Quote: ... and the plate was incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 48 hours (Fig ...
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bioRxiv - Immunology 2024Quote: ... using an Agilent 7890B gas chromatograph equipped with a 30 m DB-35ms and 5 m Duraguard capillary column (Agilent, Santa Clara, California) for separation of derivatized metabolites ...
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bioRxiv - Neuroscience 2024Quote: ... 10ug of total synaptosomal protein was plated in each well of a polyethyleneimine coated 96 well plate (n=5-6/animal) and analyzed using the MitoStress kit from Agilent (Santa Clara, CA). The MitoStress kit measures oxygen consumption rate (OCR ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). The liquid cultures were centrifuged at 3500 rpm for 5 minutes (Sorvall Legend X1R M20 rotor ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). 108 colony-forming units (CFUs ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Coverslips were then incubated with 1X DAPI for 5 min before mounting on slides using DAKO mounting media (Agilent Technologies, Cat #S302380-2). Images were captured at 60X or 100X using a Nikon C2+ confocal microscope coupled to NIS-Elements AR software (Nikon).
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bioRxiv - Bioengineering 2024Quote: ... and the plates were incubated at 37°C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Bioengineering 2022Quote: ... and the plates were incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Plant Biology 2022Quote: ... We analyzed 50 µL of each sample using an Agilent 1100-series HPLC machine with a diode array detector and a Zorbax Eclipse XDB-C18 column (4.6 x 150 mm, pore size 5 µm; Agilent Technologies, Santa Clara, USA). Desulfinated glucosinolates were separated at 40°C in a mobile phase beginning with 1.5% acetonitrile (ACN ...
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bioRxiv - Plant Biology 2022Quote: ... Samples of the final supernatant (5 μL) were automatically injected into a triple quadrupole (QqQ) LC-MS instrument (6430; Agilent Technologies, Waldbronn, Germany) equipped with an Eclipse Plus C18 column (2.1 × 50 mm ...
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bioRxiv - Plant Biology 2023Quote: ... Ten-μl B314-2 sample was injected into an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 μm, Agilent, Santa Clara, CA, USA) and eluted with the same gradient solvent system described above ...
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bioRxiv - Biochemistry 2023Quote: ... Assays were performed on an Agilent 1100 series HPLC with a reverse phase column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 × 150 mm). Solvent A was water containing 0.1% TFA and solvent B was acetonitrile containing 0.1% TFA ...
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bioRxiv - Microbiology 2023Quote: ... using two different carrier gases (argon and helium) and two stationary phases (CP- Molsieve 5 Å Plot and PoraPLOT U J&W GC columns, Agilent Technologies, Germany). Moreover ...