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Citations for Agilent :
351 - 400 of 666 citations for Recombinant NRG4 Protein GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Non-specific protein binding was blocked by incubation with 10% normal goat serum (DAKO). Primary antibodies were applied overnight at 4 ...
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bioRxiv - Cell Biology 2019Quote: ... The fusion protein was expressed in BL21 Codon Plus (DE3) RIPL Escherichia coli (Agilent) using 0.5 mM IPTG in 2xYT broth 5 hrs at 37°C and purified under native condition over Ni-NTA Agarose (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Glial Fibrillary Acidic Protein (GFAP) (Polyclonal, Dako Agilent, Santa Clara, CA, USA); mouse anti-Calbindin1 (clone CB-955 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Glial Fibrillary Acidic Protein (GFAP) (Polyclonal, Dako Agilent, Santa Clara, CA, USA); mouse anti-Calbindin1 (clone CB-955 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-Glial Fibrillary Acidic Protein (GFAP) (Polyclonal, Dako Agilent, Santa Clara, CA, USA); mouse anti-NeuN (clone A60 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-Glial Fibrillary Acidic Protein (GFAP) (Polyclonal, Dako Agilent, Santa Clara, CA, USA); mouse anti-NeuN (clone A60 ...
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bioRxiv - Cancer Biology 2022Quote: ... and the slides were blocked for 1 h with Serum Free Protein Block (Dako). Slides were incubated with antibodies directed against PARP-1 (Abcam ab194586) ...
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bioRxiv - Bioengineering 2021Quote: ... and Iba-1 (ionized calcium binding adaptor protein, 1:5000, Dako, Cat#019-19741). Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... All three proteins were expressed in BL21-Gold (DE3) competent cells (Agilent Technologies; 230132). The cells were cultured in 4 L of Luria Bertani broth (LB ...
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bioRxiv - Biochemistry 2019Quote: ... Mutant proteins were also overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) and purified using the same procedures described above ...
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bioRxiv - Microbiology 2019Quote: ... and width of protein fibrils were measured manually using PicoView1.10 software (Agilent Technologies, USA).
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bioRxiv - Molecular Biology 2021Quote: ... After blocking for 1 hour in serum-free protein blocking buffer (DAKO, catalog X0909), sections were incubated overnight at 4°C with anti–phospho-hsp25 (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were washed and blocked for 10 min (Dako protein block, Agilent, CA, USA) before being treated with primary antibodies overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were washed and blocked for 10 min (Dako protein block, Agilent, CA, USA) before being treated with primary antibodies overnight ...
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bioRxiv - Synthetic Biology 2020Quote: ... Affinity purification of biotinylated proteins was carried out in a Bravo AssayMap platform (Agilent) using AssayMap streptavidin cartridges (Agilent) ...
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bioRxiv - Developmental Biology 2019Quote: ... then blocked in serum-free protein blocking solution (Agilent Dako, Santa Clara, CA, USA) for 1hr at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... then blocked in serum-free protein blocking solution (Agilent Dako, Santa Clara, CA, USA) for 1hr at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... All Mtr4 proteins were recombinantly expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL (Stratagene) using an autoinduction protocol (Studier ...
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bioRxiv - Biochemistry 2021Quote: ... The purified S protein was separated by an SEC column (BioSEC-3, Agilent, USA) connected to an HPLC system (Analytical HPLC 1260 LC system ...
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bioRxiv - Biochemistry 2021Quote: The KlNmd4 protein was expressed in Escherischia coli BL21(DE3) Gold strain (Agilent technologies) using pMG897 plasmid and 1 L of auto-inducible terrific broth media (ForMedium AIMTB0260 ...
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bioRxiv - Immunology 2021Quote: ... Non-specific protein binding was blocked by incubation with 10% normal goat serum (DAKO). The nonspecific binding of antibodies was blocked using 10% normal goat serum (DAKO ...
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP) staining was performed (rabbit-anti-GFAP primary antibody, DAKO Z0334 ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were desalted using solid phase extraction C18 cartridges (Agilent, 5 μL bed volume). Then ...
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bioRxiv - Biophysics 2023Quote: dCas9-Cys protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with plasmid 10xHis-MBP-TEV-S ...
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bioRxiv - Biophysics 2023Quote: ... cerevisiae Cdc6 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pGEX-6P-1 wt GST-cdc6 using 400 µM IPTG for 16 h at 16 °C ...
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bioRxiv - Pathology 2023Quote: ... In addition to blocking unspecific antibody binding using Serum-Free Protein Block (X0909, Agilent) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... proteins in the supernatant were precipitated with 1% (v/v) StrataClean resin (Agilent Technologies) overnight at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... Affinity purification of biotinylated proteins was carried out in a Bravo AssayMap platform (Agilent) using AssayMap streptavidin cartridges (Agilent) ...
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bioRxiv - Physiology 2023Quote: ... the sections were blocked using Protein Block Serum-Free Blocking (X0909, Agilent, Santa Clara) and incubated with a primary antibody targeting mouse α-SMA (1:300 ...
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bioRxiv - Immunology 2023Quote: ... and rabbit polyclonal antibodies against CD-3 protein (Dako, Glostrup, Denmark; cat. no. A0452).
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bioRxiv - Cancer Biology 2023Quote: ... The protein expression was detected using the liquid DAB+ substrate chromogen system (DAKO, #K3468).
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked and permeabilized in tandem using serum-free protein block (Dako, S0909) containing 0.3% Triton-X 100 at RT for 1h ...
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bioRxiv - Bioengineering 2024Quote: ... peroxidase blocking solution and serum-free protein blocking solution were purchased from Dako (DNK). The O.C.T ...
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bioRxiv - Cell Biology 2020Quote: ... This step was directly followed by an incubation with Protein Block Serum Free (Dako X0909) for 30 minutes to block the sticky sites ...
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bioRxiv - Molecular Biology 2020Quote: ... Target proteins were identified by the horseradish peroxidase-labeled streptavidin-biotin method (1:300, DAKO). In the immunofluorescence experiments ...
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bioRxiv - Cancer Biology 2020Quote: ... Each section was treated with 4 drops of serum-free protein block (Agilent cat # X0909) for 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... Temperature-dependent protein unfolding profiles were measured using a Real-Time PCR Mx3005p machine (Stratagene). The data evaluation and melting temperature calculation were performed as described previously52’53.
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako ...
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bioRxiv - Cancer Biology 2021Quote: Ligand binding to protein was detected using DSF on an MX3005P qPCR system from Agilent Technologies as described elsewhere.37 Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The nsp14 and nsp10 proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Stratagene). The bacteria were grown in Luria–Bertani medium supplemented with 50 mg/mL kanamycin at 37 °C to an OD600 of 0.6 ...
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bioRxiv - Cancer Biology 2019Quote: ... sections were incubated for 5 min at room temperature with Protein Block Serum-Free (Dako). The tissue sections were then incubated with the anti-249T-P pAbs or TpMab-1 mAb (1 ...
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bioRxiv - Microbiology 2019Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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bioRxiv - Physiology 2019Quote: ... Slides were incubated with DAKO Protein Block Serum-Free (Agilent Technologies, Inc, Santa Clara, CA) at room temperature for 10 minutes and then with the primary antibodies diluted in Dako Antibody Diluent (Agilent Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were expressed in BL21-CodonPlus (DE3)-RIL Escherichia coli (Agilent Technologies, catalog no. 230245) at 37°C with 0.25 mM IPTG (Isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were washed and blocked for 10 min (Dako protein block; Agilent, Santa Clara, CA) before being treated with primary antibodies overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasma samples were depleted of high- and mid-abundant proteins using immune-depletion columns (Agilent Multiple Affinity Removal Column MARS3 (Agilent 5188-5218 ...
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bioRxiv - Plant Biology 2023Quote: ... Substrate proteins were induced and expressed in Escherichia coli BL21 CodonPlus(DE3)-RIL cells (Stratagene) over night at 18°C after induction with 1 mM IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used in this study were glial fibrillary acidic protein (GFAP; Source: Dako; Host ...
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bioRxiv - Immunology 2023Quote: ... VP protein masses were then measured using an in-line 6545XT QTOF system (Agilent Technologies) using the following parameters ...
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bioRxiv - Biochemistry 2023Quote: ... Secreted proteins were captured by addition of 100:1 (v/v) secretome:Strataclean resin (Agilent Technologies) (∼2 ml ...