Labshake search
Citations for Agilent :
351 - 400 of 1553 citations for Mouse KIF26B shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... Transcriptional profiling was performed on mouse colon samples using the SurePrint G3 Mouse GE8×60K Microarray kit (design ID:028005, Agilent Technologies). Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were then co-transfected with two helper plasmids (pHelper and pAAV-RC; Stratagene) and one of the pAAV plasmids (pAAV-NT ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The control plasmid was pCMV-3Tag-3A (pCMV) (Agilent Technologies, Santa Clara, CA, U.S.A.). HEK293 cells were seeded at a density of 1 × 104 cells per well in either 96- or 24-well plates and transfected 24 hours later using JetPRIME (Polyplus Transfection ...
-
bioRxiv - Molecular Biology 2021Quote: ... template WT plasmid was digested by the addition of 1 μL DpnI (Agilent Technologies) for 5 min at 37 °C ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were propagated in Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, CA), after growth in Lysogeny broth (LB ...
-
bioRxiv - Molecular Biology 2019Quote: ... All sequence changes to the plasmid were accomplished by site directed mutagenesis (QuikChange, Agilent). The histone H3 plasmid from this reference contains an H3-G102A mutation ...
-
bioRxiv - Microbiology 2021Quote: ... The pShuttle-ACE2 plasmids were then electroporated 440 into BJ5183 AD-1 cells (Agilent), which were pretransformed with pAdEasy-1 to facilitate recombination with the pShuttle-CMV vector ...
-
bioRxiv - Biochemistry 2021Quote: ... Sequence-verified plasmids were transformed into ArcticExpress (DE3) competent cells (Agilent Technologies, California, USA) for protein expression ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were then co-transfected with two helper plasmids (pHelper and pAAV-RC; Stratagene) and one of the pAAV plasmids (pAAV-DCUg-NT ...
-
bioRxiv - Cell Biology 2021Quote: ... This plasmid was used as template for site-directed mutagenesis (QuickChange kit, Agilent Technologies) with the following oligonucleotides:
-
bioRxiv - Systems Biology 2021Quote: ... For plasmid propagation chemically competent Escherichia coli XL1-Blue (Agilent Technologies, Santa Clara, CA) cells were used which were grown in lysogeny broth (LB ...
-
bioRxiv - Molecular Biology 2020Quote: ... The plasmid was transformed into Escherichia coli strain BL21-Codon-plus(DE3)-RIL (Stratagene). Bacteria was grown in LB broth in a shaker at 37°C until reaching the log phase (A600nm between 0.4 and 0.5) ...
-
bioRxiv - Biochemistry 2022Quote: ... The GYG1p.Y195F mutant was generated from this plasmid using the QuickChange mutagenesis kit (Stratagene). Codon optimised genes for GYS1 and aa 264/294-350 GYG1 (GYG1ΔCD ...
-
bioRxiv - Biophysics 2022Quote: ... Channel mutations were introduced into this plasmid using the QuickChange Lightning kit from Agilent. All portions of the resulting construct that were subject to PCR were confirmed by DNA sequencing.
-
bioRxiv - Microbiology 2021Quote: ... Mutated plasmids were generated by Quick-Change Site-directed Mutagenesis (Table 1) (Agilent Technology) based on the secondary structure model of the 5’UTR of A3G mRNA (40 ...
-
bioRxiv - Biochemistry 2021Quote: pGEX4T-3 expression plasmids were transformed into BL21-Codon Plus RIPL competent cells (Agilent). Protein expression was induced at 20°C for 3.5 hr ...
-
bioRxiv - Synthetic Biology 2020Quote: Mutagenesis was performed on the pET21b(+)-TrpR-Wt plasmid using the QuikChange technique (Stratagene) following the manufacturer’s protocol.
-
bioRxiv - Biophysics 2019Quote: ... The recombinant plasmid was subsequently transformed into Escherichia coli BL21 (DE3) cells (Agilent Technologies) for protein expression ...
-
bioRxiv - Microbiology 2020Quote: ... High concentration plasmid solutions were produced using XL1-Blue Competent Cells (200249, Agilent technology) and plasmid extraction was performed using Qiagen Plasmid Maxikit (12163 ...
-
bioRxiv - Cell Biology 2022Quote: ... Amplified cDNA was cloned into pSC plasmid (StrataClone Blunt PCR Cloning Kit, STRATAGENE, 240207). Then ...
-
bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
-
bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
-
bioRxiv - Molecular Biology 2024Quote: ... The expression plasmids were transformed into Escherichia Coli strain BL21-Codonplus(DE3)-RIL (Stratagene). The expression and purification of each protein was performed as described previously.77 Briefly ...
-
bioRxiv - Biochemistry 2024Quote: The codon-optimized plasmid for hPLIN1 was expressed in Escherichia coli ArcticExpress (DE3) (Agilent) while codon optimized plasmids hPLIN2 and hPLIN3 were expressed in E ...
-
bioRxiv - Biochemistry 2023Quote: ... each plasmid was transformed into BL21-CodonPlus(DE3)-RIPL competent cells (Agilent Technologies, #230280). A single colony was picked and inoculated in 1 mL of terrific broth (TB ...
-
bioRxiv - Physiology 2023Quote: Plasmid constructs used for transient transfection were amplified using XL10-gold ultracompetent cells (Agilent Technologies Inc. ...
-
bioRxiv - Biophysics 2023Quote: ... The resultant recombinant plasmids were transformed into ultra-competent ArcticExpress (DE3) cells (Agilent Technologies). ArcticExpress cells are derived from BL21(DE3) ...
-
bioRxiv - Biochemistry 2023Quote: ... The point mutation plasmids were generated using QuikChange II kit (Agilent, cat no. 200521) with corresponding primers ...
-
bioRxiv - Biochemistry 2023Quote: The bacterial expression plasmid (pk19pA-Apex2-6xHis) was transformed into Bl21DE3 Gold (Stratagene# 230132) according to manufacturer instruction ...
-
bioRxiv - Neuroscience 2024Quote: ... and the constructed AAV-vector plasmid were transfected into AAV-293 cells (#240073, Agilent) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... primary antibody was produced in-house from HB 65 mouse anti-nucleoprotein and diluted 1:2500 followed by HRP-conjugated rabbit anti-mouse diluted 1:500 (Dako, Glostrup, Denmark). The titration was repeated on a different day and TCID50 titres were calculated using Reed and Muench (29).
-
bioRxiv - Cancer Biology 2021Quote: ... mouse anti-CD44 (1:50, clone DF1485, Dako, M7082), rabbit anti-EGFR (prediluted ...
-
bioRxiv - Molecular Biology 2020Quote: ... and polyclonal goat anti-mouse IgG HRP (Dako, Agilent). The oxidants used in this study were 30% hydrogen peroxide (H2O2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and polyclonal goat anti-mouse IgG HRP (Dako, Agilent). The oxidants used in this study were 30% hydrogen peroxide (H2O2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Alkaline Phosphatase/RED Rabbit/Mouse was used (K5005, Dako). For xenograft slides ...
-
bioRxiv - Cell Biology 2020Quote: ... or goat anti-mouse IgG/HRP (1:5000, Dako) for 1 h at room temperature before being washed ...
-
bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase-conjugated anti-mouse (Dako, #P0447; 1:4,000) or anti-rabbit IgG (gamma-chain specific ...
-
bioRxiv - Genetics 2021Quote: ... and mouse-anti-Ki67 (Agilent, M7248, dilution 1:100). Goat-anti-mouse-488 and Goat-anti-rabbit-568 were used as secondary antibodies and sections were counterstained with DAPI ...
-
bioRxiv - Cancer Biology 2020Quote: ... or anti-mouse secondary antibody (Dako Cytomation, Carpinteria, CA). Signal amplification was achieved using a commercially available tyramide- based avidin/biotin amplification system (Dako Cytomation ...
-
bioRxiv - Microbiology 2021Quote: ... then 1:1500 goat anti-mouse IgG-HRP (Dako); 1:1000 anti-HA (Roche) ...
-
bioRxiv - Microbiology 2021Quote: ... then 1:1500 goat anti-mouse IgG-HRP (Dako). Membranes were washed for 3 × 5 mins in TBST after each antibody step ...
-
bioRxiv - Cell Biology 2022Quote: ... or goat anti-mouse (DAKO, cat# P0447; 1:4,000).
-
bioRxiv - Biochemistry 2022Quote: ... HRP-conjugated anti-rabbit and anti-mouse antibody (DAKO-Agilent ...
-
bioRxiv - Neuroscience 2021Quote: ... Mouse anti-Ki67 (1:400, M724029-2, Agilent, UK) was used to label proliferative cells and rabbit anti-cleaved-Caspase3 (1:40 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Horseradish Peroxidase conjuguated anti-mouse IgG (Dako, 1/5000) and anti-rabbit IgG (Dako ...
-
bioRxiv - Molecular Biology 2021Quote: ... and HRP-conjugated secondary antibodies (anti-mouse (Dako, P0447) or anti-rabbit (Dako ...
-
bioRxiv - Cell Biology 2019Quote: ... or goat anti mouse-HRP (1:100, P0447, DAKO) (type II collagen ...
-
bioRxiv - Developmental Biology 2019Quote: ... or mouse anti-human CD31 (Dako, M0823, 1:50) overnight at 4°C and then goat anti-mouse Alexa488 (ThermoFisher Scientific ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... respectively) was measured using Whole Mouse Genome Microarrays (Agilent), as described in Turner et al ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse clone CR3/43 (1:20, Agilent, M077501–2). Sections were incubated for 1 hour at room temperature with secondary antibodies (1:1000) ...