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Citations for Agilent :
351 - 400 of 842 citations for Ephrin type A receptor 8 EphA8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The Lys162Met variant for all AURKA biosensors analysed in the study were obtained from the corresponding wild-type constructs by QuikChange site-directed mutagenesis (Stratagene) with the following primers ...
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bioRxiv - Developmental Biology 2019Quote: ... was created by introducing the point mutation in wild-type vasa cDNA sequence by site directed mutagenesis using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). The transgene is subsequently cloned downstream of GFP into the pUASK10attB vector (gift of Beat Suter) ...
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bioRxiv - Developmental Biology 2019Quote: ... pBMN-HIPK4-Y175F-3xFLAG-IRES-mCherry was later generated by site-directed mutagenesis of the wild type construct using the primers 5’-CGCTATGTGAAGGAGCCTTTCATCCAGTCCCGCTTC TAC-3’ and 5’-GTAGAA GCGGGACTGGATGAAAGGCTCCTTCACATAGCG-3’ and PfuUltra II Fusion polymerase (Agilent).
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bioRxiv - Microbiology 2021Quote: ... D950N) were prepared from wild-type SARS-CoV-2 spike using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: ... Parkin mutant plasmids were cloned beginning with a Homo sapiens wild type eGFP-parkin vector [36] and performing PCR point mutagenesis (Quikchange Mutagenesis kit: Stratagene) utilizing primers ordered from Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: Cys to Ala substitution mutations were introduced at specific sites in the ectodomain of wild type toll by using QuickChange II XL site-directed mutagenesis kit (Stratagene) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... These genes were cloned into an Adenovirus type 5 replication-defective E1/E3 deleted vector using the Ad-Easy Adenoviral Vector System (Agilent). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... All K-Ras secondary mutant and wild-type K-Ras constructs were generated by a PCR based strategy using a site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: 2 μl of extracted metabolite samples were separated on a Cogent Diamond Hydride Type C Column of 1200 liquid chromatography (Agilent) which was coupled to an Agilent Accurate-Mass 6220 Time-of-Flight spectrometer ...
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bioRxiv - Genetics 2023Quote: ... The C211G mutant was generated by site-directed mutagenesis of the wild-type vector using the QuikChange II protocol (Agilent). To generate the Myc-tagged versions ...
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bioRxiv - Biophysics 2023Quote: ... A clone encoding tagless wild type PUS1 was then generated from PUS1D134A by converting alanine 134 back to wild type aspartate 134 using a QuickChange II XL kit and protocol (Agilent).
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bioRxiv - Bioengineering 2023Quote: ... The library of CjCas9 variants was produced by random mutagenesis of the wild-type sequence using error-prone PCR approach (GeneMorph II kit from Agilent) (for primers ...
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bioRxiv - Molecular Biology 2023Quote: ... mutant plasmids encoding the different RNA exosomopathy amino acid substitutions were generated by site-directed mutagenesis of the respective wild-type plasmids (pAC3656, pAC3652, pAC3482) using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the desired missense mutations as previously described [33 ...
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bioRxiv - Cell Biology 2023Quote: Basal oxygen consumption rates in kidneys from non-diabetic and diabetic wild-type and Angptl4-/- mice were characterized using the Seahorse Flux Analyzer (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Extracted metabolites were separated on a Cogent Diamond Hydride Type C column of 1200 liquid chromatography coupled to an Mass 6220 TOF spectrophotometer (Agilent). Ion abundances of metabolites were estimated using Profinder 8.0 and log2 fold changes were analyzed and plotted using Gene Cluster 3.0 and Java Treeview.
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bioRxiv - Biochemistry 2023Quote: Oxygen consumption rates (OCR) of wild-type C2C12 under various concentrations of uncouplers were measured with Seahorse XFe24 analyzer (Agilent). Experiments were conducted under the same process as explained above ...
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bioRxiv - Molecular Biology 2020Quote: Cybrid cells were plated according to manufacturer’s instructions on 8-well Seahorse XFp plates (Agilent, 103010-100). The day of the assay ...
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bioRxiv - Physiology 2021Quote: ... Génopole Toulouse Midi-Pyrénées) using Sureprint G3 Mouse GE v2 microarrays (8×60K, design 074809, Agilent Technologies), according to the manufacturer's instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 200 μl of distilled water was added to the sensor-containing Seahorse 8-well fluxpaks (Agilent Technology), which were coated with Poly-D-Lysine ...
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bioRxiv - Biochemistry 2019Quote: ... all samples were processed and hybridized to SurePrint G3 Mouse Gene Expression 8 × 60 K (Agilent Technologies). Fluorescence was detected using Agilent DNA Microarray Scanner ...
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bioRxiv - Neuroscience 2023Quote: Neurospheres were removed from 96-well plates and pipetted into 8-well Seahorse XF HS miniplates (Agilent) coated with 15 µg/mL poly-L-ornithine and 10µg/mL laminin ...
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bioRxiv - Neuroscience 2023Quote: ... 0-8 min with 0.1% formic acid) and a C-18 column (4.6 × 50 mm, 1.8 μm) (Agilent). Each compound was assessed in triplicate ...
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bioRxiv - Neuroscience 2023Quote: ... 0-8 min with 0.1% formic acid) and a (C-18 column (4.6 × 50 mm, 1.8 μm) (Agilent). The photoproducts of DEAC-OXM were generated and analyzed in an analogous manner ...
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bioRxiv - Immunology 2024Quote: Initial experiments were conducted with an 8-well Seahorse XFp extracellular flux analyser (Seahorse Bioscience, Inc, USA) to determine cell seeding density and FCCP concentration (Supplementary Data) ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Cancer Biology 2021Quote: ... immunohistochemical staining was done with an antibody against human Ki67 (M7240, DAKO). 10 μm-thick tumor cryosections were fixed with a 4% PFA solution ...
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bioRxiv - Genomics 2020Quote: ... pre-capture PCR and SureSelectXT Human All Exon V5+UTR baits (Agilent). Post-capture fragments were amplified by PCR ...
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bioRxiv - Genomics 2020Quote: ... Lysates and positive controls (Human Universal Reference RNA - uhrRNA (Agilent Cat# 740000), and Human Brain Total RNA brRNA (ThermoFisher AM7962)) ...
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bioRxiv - Cell Biology 2019Quote: ... Human KIF4A was amplified using Pfu hot start turbo polymerase (Agilent Technologies). Mammalian expression constructs were made in pcDNA5/FRT/TO (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... The antibodies used were the polyclonal rabbit anti-human β2M antibody (Dako) (used at 1/1000) ...
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bioRxiv - Immunology 2020Quote: ... Antibodies used in IHC assays include: mouse-anti-human CD3 (Dako, F7.2.38), rabbit-anti-human Granzyme B (eBiosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-human CD20 (Dako, clone L26, 1:1000, pH 6 retrieval), mouse anti-human FOXP3 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were treated with polyclonal rabbit anti-human IgG (Agilent, A0423) at 7.5 μg/mL for 8 or 24 h ...
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bioRxiv - Genetics 2021Quote: ... we used the i) Agilent SureSelectXT Human All Exon V6 (Agilent Technologies), ii ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were incubated with rabbit anti-human PGP 9.5 (DAKO 1:100) overnight at 4°C.
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bioRxiv - Cancer Biology 2022Quote: The human CDK1 S39A mutation was introduced using Quickchange (Agilent, cat #600670) using specific primers ...
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bioRxiv - Cancer Biology 2021Quote: ... The exons were captured using SureSelect XT2 Human All Exon V6 (Agilent), and sequenced by paired-end 75 bp sequencing on HiSeq4000 (Illumina) ...
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bioRxiv - Immunology 2022Quote: ... GFAP was detected with polyclonal rabbit-anti human GFAP (1:500, Agilent) and donkey anti-rabbit IgG AF55 detection (1:500 ...
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bioRxiv - Immunology 2019Quote: ... Sections were incubated with mouse anti-human CD68 (#M0814, Clone KP1, Dako) antibody (1:100 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-human PECAM-1 (CD31) (M0823, Agilent Dako, Santa Clara, CA), GAPDH mouse anti-human (10R-G109b ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-human PECAM-1 (CD31) (M0823, Agilent Dako, Santa Clara, CA), GAPDH mouse anti-human (10R-G109b ...
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bioRxiv - Immunology 2020Quote: ... or rabbit anti-human IgA/HRP (Dako: cat. P0216, 1:5’000 dilution). Assays were developed by addition of 3,3’,5,5’-Tetramethylbenzidine (TMB ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibodies were detected using HRP-labelled rabbit anti–human IgG (Dako) or anti-hamster IgG and TMB (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: Immunohistochemistry on human (antigen retrieval by Target Retrieval Solution pH6.0 (DAKO #S1699)) and mouse fetal tissue was performed using Autostainer (Dako ...
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bioRxiv - Genomics 2021Quote: ... MAQCA is the Quantitative PCR Human Reference Total RNA (#750500, Agilent technologies), extracted from cell lines representing different human tissues ...
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bioRxiv - Immunology 2020Quote: ... Monoclonal mouse IgG1 anti-human CD32 (clone KB61) was purchased from Dako, Santa Clara ...
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bioRxiv - Molecular Biology 2022Quote: ... Whole-exome sequencing was performed using SureSelect Human All Exon V7 (Agilent) according to manufacturer’s protocol and sequenced on an Illumina NextSeq 500 (paired-end 150 bp reads) ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with primary antibodies against human CD31 (1:50, JC70A, Dako), PDGFRβ (1:100 ...
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bioRxiv - Genetics 2023Quote: ... Microdeletions were identified by array-CGH (Human Sureprint 2×105K, Agilent technologies) and confirmed with semiquantitative PCR ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody was mouse anti-human Aβ (Dako #M0872; 1:400) and the secondary antibody was Vectastain ABC kit anti-mouse secondary antibody (Vector Laboratories #PK-4002 ...