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Citations for Agilent :
351 - 400 of 748 citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... slides were washed three times in PBS for 5 minutes and mounted in DAKO fluorescence mounting medium (Agilent).
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bioRxiv - Microbiology 2023Quote: ... rabbit F(ab’)2 anti-human C1q-FITC (both at 5 µg/ml, Dako as described in (9)) ...
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bioRxiv - Systems Biology 2023Quote: ... its column was chosen as DB-5 (30 m × 0.25 mm × 0.25 µm) (Agilent, Santa Clara, CA, USA) with reverse phase property ...
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bioRxiv - Bioengineering 2023Quote: All kinetic data were obtained using either a BioTek Synergy H1 or Cytation 5 plate reader (Agilent Technologies). HEX was measured with an excitation peak of 533 nm and an emission peak of 559 nm with a gain of 80 to 100 to ensure fluorescence values were within the linear range of detection ...
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bioRxiv - Genetics 2023Quote: ... slides were carefully rinsed 3 × 5 min with PBS and slides were mounted with Fluorescent Mounting Medium (Dako) and examined under fluorescence using a Zeiss microscope equipped with an AxioCam HRm camera.
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bioRxiv - Biochemistry 2023Quote: ... Peptide purity was assessed with an analytical column (Agilent, ZORBAX 300SB-C18, 4.6 x 150 mm, 5 μm) at a flow rate of 1 mL/min over a 0-90% B gradient in 30 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... equipped with a guard column (EC-C18; 3.0 mm internal diameter, 5 mm length, 2.7 μm particle size; Agilent), was conditioned for 20 minutes with 95 % mobile phase A and 5 % B ...
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bioRxiv - Cancer Biology 2023Quote: ... on a HPLC column (PLRP-S 1000 Å, 5 mm, 50×2.1 mm, Agilent Technologies, Santa Clara, CA). The following solvents were used ...
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bioRxiv - Neuroscience 2023Quote: ... The plate was thoroughly washed 5 times with PBS-T and 100 μL of TMB-Blue substrate (DAKO) was added followed by incubation in the dark for 5-10 min ...
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bioRxiv - Cell Biology 2023Quote: ... and a 5 m guard capillary column coupled to an Agilent 5975C inert mass selective detector (Agilent Technologies). Electron impact ionization (EI ...
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bioRxiv - Biochemistry 2023Quote: ... HPLC was performed on an Agilent 1260 infinity II, equipped with a C-18 column (Mackerey-Nagel, 5 µm, ref 760.100.40) using acetonitrile (HPLC grade, Agilent) and ddH2O (0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... at 5 mM for 2 h or by UV irradiation at 20 mJ/cm2 using Stratalinker 1800 (Stratagene) followed by 2 h incubation at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Dual luciferase activity was measured using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent, Santa Clara, CA). Normalized luciferase activity was determined by calculating Firefly to Renilla signal ratio.
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 2h with primary antibody diluted in Blocking Buffer with 5% Goat Serum (Dako #X090710) (antibodies are dilutions used are listed in Table 2) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The experiment was conducted using a BioTek Synergy HTX Multi-Mode Microplate reader with Gen 5 software (Agilent), set at a temperature of 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... ITO slides were then stained for H&E and imaged using the Cytation 5 (Agilent, Santa Clara, CA) and Epsilon scanner at 3000 dpi to select regions of interest using the open-source program ...
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bioRxiv - Plant Biology 2024Quote: ... The column was an Eclipse Plus C18 column (250 mm x 4.6 mm, 5 µm; Agilent Technologies, USA) with a security guard ultra C18 guard column (4.6 mm x 2 mm ...
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bioRxiv - Molecular Biology 2019Quote: ... The mixture was pre-incubated at 37°C for 5 min and AffinityScript Multiple Temperature Reverse Transcriptase (Agilent Technologies) was added to each toeprinting reaction at a final content of 10.2% (v/v ...
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bioRxiv - Evolutionary Biology 2021Quote: ... An Agilent J&W HP-5 column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Agilent Technologies, USA) was used in the GC ...
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated in secondary antibody solutions (TBS containing 0.1 % Tween-20 and 5 % BSA, HRP-conjugated secondary antibodies (1:5000, Dako)) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were desalted online by reversed-phase chromatography on a Poroshell 300SB C3 column (1.0 × 75 mm, 5 μm, Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Separation occurred with a HP-5 ms capillary column coated with 5% phenyl-95% methylpolysiloxane (30 m×250 µm i.d., 0.25 µm film thickness, Agilent Technologies).
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bioRxiv - Molecular Biology 2019Quote: EMSAs were performed as described and cross-linked for 5 min (∼720 mJ) in a UV Stratalinker 1800 (Stratagene). Samples were taken up in 2x SDS loading buffer and boiled for 5 min before being loaded on a 7.5 % denaturing polyacrylamide gel and run for 1-2 hours in 10 % SDS-running buffer at 130 V ...
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bioRxiv - Microbiology 2019Quote: ... equipped with an HP-5 capillary column (60 m × 0.25 mm × 0.25 μm, Agilent Technologies, Inc., Santa Clara, CA). Pure helium (99.9999 % ...
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bioRxiv - Plant Biology 2019Quote: ... An HP-5MS capillary column (5%phenyl-methyl-siloxane, 30-m, 250-mm, and 0.25-mm film thickness; Agilent) was used with helium carrier gas at 2 mL/min ...
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bioRxiv - Synthetic Biology 2020Quote: ... Ten microliters of each diluted sample were injected and sample separation was achieved using a Zorbax Eclipse Plus C18 guard column (4.6 cm×12.5 cm, 5 μm packing, Agilent Technologies) connected to a Zorbax Eclipse Plus C18 column (4.6 mm × 100 mm ...
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bioRxiv - Biophysics 2020Quote: ... They were then stained for 5 min with 4,6-diamidino-2-phenylindole (DAPI) and mounted using fluorescence mounting medium (DAKO, Agilent). Pictures were taken with an UltraVIEW ERS spinning disk confocal microscope (Zeiss 63X/1.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... the TMT-labelled peptides were fractionated using high pH reverse phase chromatography on a C18 column (150 × 2.1 mm i.d. - Kinetex EVO (5 μm, 100 Å)) using an HPLC system (Agilent, LC 1260 Infinity II ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μg of pooled total RNA were tested for RNA quality on an ABI2000 Bioanalyzer (Agilent; Santa Clara, CA), labeled ...
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bioRxiv - Biochemistry 2020Quote: Concentrated protein samples were diluted to 0.02 mg/mL in 0.1% formic acid and 50 μL was injected on to a 2.1 mm x 12.5 mm Zorbax 5 μm 300SB-C3 guard column (Agilent Technologies) housed in a column oven set at 40°C ...
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bioRxiv - Microbiology 2021Quote: ... with a 30 x 0.25 mm (0.25 µm thickness) DB-5 MS capillary column (Agilent J & W capillary GC) and coupled to a Polaris Q benchtop Ion Trap Mass Spectrometer ...
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bioRxiv - Biophysics 2020Quote: ... in 20 min at a flow rate of 0.4 mL min-1 using a Zorbax Narrow Bore (2.1×50 mm, 5 μm) C18 column (Agilent Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... HPTS and RuBPY fluorescence were monitored for 17 h using a Cytation 5 device (BioTek, Agilent, Winooski, VT, USA). Excitation was provided by a monochromator ...
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bioRxiv - Cancer Biology 2022Quote: ... and read by brightfield and fluorescence imaging on a BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies) every six hours for a total of six days ...
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bioRxiv - Biochemistry 2022Quote: ... The gas chromatograph was equipped with a DB-5 ms column (30 m × 0.25 mm, film thickness 0.25 μm; including 10 m DuraGuard column, Agilent Technologies) and a GC inlet liner (ultra inert ...
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bioRxiv - Synthetic Biology 2022Quote: ... fitted with a guard column (Zorbax Eclipse XDB-C18, 2.1 x 5 mm 1.8 μm, Agilent part # 821725-903) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 9.0 for 30 minutes followed by a 5-minute incubation in DAKO Peroxidase Block (DAKO, Catalog No. S2001) to block endogenous peroxidase activity ...
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bioRxiv - Genomics 2022Quote: ... RT was performed with a specific primer (5′-CCTACACGACGCTCTTCC-3′) using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). RNA degradation was performed by incubating the RT mixture with 10% 1 M NaOH (2μl of RT mixture ...
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bioRxiv - Plant Biology 2021Quote: Quantitative analysis was performed using a HP-5MS capillary column (5% phenyl-methyl-siloxane, 30 m x 250 mm, 0.25 mm film thickness; Agilent) with helium carrier gas at 1.5 mL/min ...
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bioRxiv - Microbiology 2021Quote: ... samples (5 uL) were injected onto a ZORBAX RRHD Extend-C18 column (2.1 x 150 mm, 1.8 µm; Agilent Technologies) with a ZORBAX SB-C8 (2.1 mm × 30 mm ...
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bioRxiv - Neuroscience 2020Quote: ... and loaded onto a ZORBAX Extend 300 C18 columns (4.6 mm x 250 mm with 5 µm beads) from Agilent. An Agilent 1100 HPLC system was used to carry out the fractionation ...
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bioRxiv - Microbiology 2022Quote: ... containing peptaibols was carried out by semi-preparative HPLC on an SSI system (Teledyne SSI Lab Alliance Series III pump system and Series 1500 Photodiode Array Detector) with an Eclipse XDB-C18 column (9.4 × 250 mm, 5 μm, Agilent). Acetonitrile (A ...
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bioRxiv - Microbiology 2022Quote: ... After centrifugation plate was incubated for 5 minutes before being loaded into a Seahorse XF96 Extracellular Flux Analyzer (Agilent). Basal respiration was measured over six hours with 36 ten-minute protocol cycles including a ...
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bioRxiv - Biochemistry 2022Quote: ... Sample from each PNT3 variant at 5 mg mL-1 in buffer B containing 6M GDN was injected onto an AdvanceBio SEC 2.7 µm (Agilent) SEC column ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with 1 × blocking buffer (5% goat serum [#X0907, Dako], 2.5% BSA, 0.1% Triton X-100 in PBS) (38) ...
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bioRxiv - Microbiology 2019Quote: ... A Zorbax SB-C8 reversed-phase column (5 μm, 2.1 × 50 mm) was obtained from Agilent (Palo Alto, CA). The LC eluent was introduced into the ESI source of the mass spectrometer ...
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bioRxiv - Plant Biology 2019Quote: ... 25 μg of peptides were loaded unto 20 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Zoology 2020Quote: ... GC-SSR was performed on a GC-7890 Agilent Gas Chromatogram equipped with a DB-5 column (30 m, 0.25 mm; 0.25 μm; Agilent Technologies) and connected with a transfer line and a temperature control unit from Syntech ...
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bioRxiv - Biophysics 2020Quote: ... After rinsing for 5 minutes in PBS they were incubated with secondary antibody (Rabbit Anti-Mouse 1/200, Dako) for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... HPLC with DAD was performed on an Agilent 1200 Series instrument equipped with an Eclipse Plus C18 column (4.6 × 250 m; particle size = 5 μm; Agilent). Activities of the enzymes were estimated as Turnover Number nmoles of metabolized product / nmol of CYP / min (min-1) ...