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Citations for Agilent :
351 - 400 of 3941 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Comparator an d frozen shoulder capsular tissue sections were obtained through deparaffinization and target retrieval steps (high pH, heat-mediated antigen retrieval) using an automated PT Link (Dako). Antibody staining was performed using the EnVision FLEX visualization system with an Autostainer Link 48 (Dako) ...
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bioRxiv - Developmental Biology 2023Quote: For IHC staining slides were thawed and antigen-retrieval performed by incubating the sections with preheated Target Retrieval Solution (DAKO) 20 min at 70°C ...
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bioRxiv - Cell Biology 2022Quote: ... Immediately after the slides were immersed in pre-heated (90-100 degree Celsius) 1X antigen retrieval solution (Dako, cat # S1699) for 25 min ...
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bioRxiv - Cancer Biology 2023Quote: The 4-6um thick TMA sections were deparaffinized and antigens were unmasked using EnVision FLEX Target Retrival Solution Low pH (Dako Agilent) in PT link device (PT200 ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Biochemistry 2019Quote: The genomic DNA template was amplified by the polymerase chain reaction (PCR) using Pfu Ultra High-Fidelity DNA polymerase (Agilent Technologies). Each 50 μL reaction consisted of genomic DNA (~12 ng) ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed in triplicate using master mix (Brilliant III Ultra-Fast SYBR Green QPCR Master Mix, Agilent Technologies) and a real-time PCR system (AriaMax Real PCR System ...
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bioRxiv - Bioengineering 2021Quote: ... Mutations were cloned into the expression vector by polymerase chain reaction (PCR) using a PFUultra II Hotstart PCR Master Mix (Agilent Technologies). The obtained PCR products were subsequently treated with DPNI (New England Biolabs ...
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bioRxiv - Physiology 2021Quote: Two truncated recombinant fibrinogen α-chain variants (α220 and α390) were produced using QuikChange II Site-Directed Mutagenesis Kits from Agilent Technologies (Stockport ...
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bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
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bioRxiv - Immunology 2020Quote: ... The RM19R Fab heavy chain plasmid was made introducing two stop codons following residue D234 in the RM19R IgG1 heavy chain vector using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). The RM19R Fab was expressed in HEK293F cells (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Fab expression vectors were made by introducing two stop codons following residue D234 (Kabat numbering 57) in the IgG heavy chain vectors using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). Sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Cell Biology 2019Quote: ... and indels were determined by amplification of the sgRNA target site by polymerase chain reaction using Herculase II Fusion DNA Polymerase (Agilent biotechnologies) and Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: Short-chain fatty acids were analyzed by gas chromatography with a flame ionization detector (GC-FID 7890 A Agilent Technologies Inc.) with a fused silica capillary column (Nukon SUPELCO ...
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bioRxiv - Systems Biology 2023Quote: ... bile acids and short chain fatty acids analyses were performed by LC-MS/MS with a 1260 UHPLC (Ultra-High Performance Liquid Chromatography) (Agilent Technologies) coupled to a QQQ 6410 (Agilent Technologies ...
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bioRxiv - Biophysics 2021Quote: ... Norland Products) and three minutes curing by intense UV light at 265 nm (UV Stratalinker 2400, Stratagene). Freshly cleaned and assembled chambers were directly used for experiments.
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bioRxiv - Biochemistry 2024Quote: ... followed by exposure to UV light (365 nm) in a UV cross-inker (Stratagene, UV Stratalinker 1800) placed in a cold room for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... followed by exposure to UV light (365 nm) in a UV cross-inker (Stratagene, UV Stratalinker 1800) placed in a cold room for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with primary antibodies directed against CD31 (M0823, DAKO), GFP (ab6556 ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-µm sections were cut and antigen retrieval was carried out by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies) for Ki67 ...
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bioRxiv - Neuroscience 2020Quote: ... Heat antigen retrieval was performed by steaming at 98°C in target retrieval solution pH 6.1 (Dako, Carpinteira, CA, USA #S1699) for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... heat mediated antigen retrieval was performed for 3 min at 125°C in citrate pH 6.0 target retrieval solution (Dako Cat# S2369) using a decloaking chamber (Biocare Medical) ...
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bioRxiv - Cancer Biology 2022Quote: Formalin-fixed samples tissue microarrays of EwS-samples and normal tissues were stained for GPR64 after antigen retrieval using Target Retrieval Solution (Fa.Agilent Technologies, S1699) with anti-GPR64 (purified using Mouse TCS Antibody Purification Kit (ab128749 ...
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bioRxiv - Neuroscience 2021Quote: ... 3 μm paraffin-embedded tissue sections were either dewaxed and subjected to antigen retrieval treatment with Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent) or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005 ...
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bioRxiv - Neuroscience 2021Quote: ... or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005, Dako-Agilent) for 20 min at 97°C using a PT Link (Dako – Agilent) ...
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bioRxiv - Immunology 2019Quote: ... Antigen retrieval was performed either with Proteinase K (for F4/80) or boiling the sections in a high pH antigen retrieval buffer (Dako Target retrieval Solution, pH 9, Dako, Denmark) (for Podocalyxin ...
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bioRxiv - Immunology 2021Quote: ... Slides were then rinsed with double distilled water (ddH2O) and boiled in 1X Dako pH9 antigen retrieval solution (Agilent, S236784-2) for 10 min ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed on 4-μm thick formalin-fixed paraffin-embedded (FFPE) whole tissue serial sections following antigen retrieval (Target Retrieval Solution, pH 6.1; Dako, Carpinteria, CA). Immunostains performed included Connexin 26 (Cx26 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Cancer Biology 2023Quote: The 4-6um thick TMA sections were deparaffinized and antigens were unmasked using EnVision FLEX Target Retrival Solution Low pH (Dako Agilent) in PT link device (PT200 ...
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bioRxiv - Molecular Biology 2024Quote: ... paraffin sections were rehydrated through graded alcohols and antigen retrieval was performed using Target Retrieval Solution (Dako, Carpinteria, CA, United States), pH 6.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5µm sections were deparaffinized with xylene and rehydrated through graded ethanol washes followed by antigen retrieval in sodium citrate buffer (10 mM, pH 6.0) with a pressure cooker (Dako, Carpinteria, CA), blocked ...
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bioRxiv - Evolutionary Biology 2021Quote: ... segetum to the synthetic mixture of C6-C10 straight chain acids were recorded on an Agilent 7890 gas chromatograph equipped with a flame ionization detector (FID) (Agilent Technologies, USA) and an electroantennographic detector (EAD ...
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bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
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bioRxiv - Cancer Biology 2021Quote: ... suspended in saline in 12 mm glass vials and 2H-MR spectra acquired using a 16 mm 2H single loop surface coil (DOTY Scientific) at a Varian 14.1T vertical MR scanner (Agilent Technologies). A pulse-acquire sequence (TR=260ms ...
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bioRxiv - Genetics 2019Quote: ... coversliped and exposed to 365-nm UV light for 30 min using a UV Stratalinker 2400 transilluminator (Stratagene). Slides were rinsed with H20 and drained ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with HRP-conjugated rabbit anti-mouse antibody (DaKo, P0161) diluted 1:5000 in blocking buffer for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with primary antibodies CD31 (1:200, M082329-2, DAKO), NG2 (1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were stained with antibodies: Guinea pig anti-insulin (1:100, Dako) followed by anti-guinea pig-Alexa Fluor® 555 (1:100 ...
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bioRxiv - Pathology 2023Quote: ... cells were incubated in anti-BrdU primary monoclonal antibody (DAKO, Glostrup, Denmark), and fluorescein isothiocyanate-conjugated anti-mouse secondary antibody ...
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bioRxiv - Immunology 2022Quote: Specific amino acid changes on envelope clones and CAP206-CH12 heavy chain CDRH3 were introduced using the QuikChange Lightning Site-Directed Mutagenesis Kit (catalog #210519, Agilent Technologies, CA, USA). Mutations were confirmed by sequence analysis.
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bioRxiv - Cancer Biology 2023Quote: ... and hypoxanthine guanine phosphoribosyl transferase (HPRT) were assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR, Mx3005P QPCR System (Agilent, Santa Clara, CA). After 24 or 48 h of incubation in normoxia ...
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence microscopy was performed on de-paraffinized tissue sections that received heat-induced antigen retrieval using Target Retrieval Solution (Agilent Dako, S169984-2). After washing and permeabilization in TBS-T universal buffer (0.2% Triton X-100 in tris-buffered saline) ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were then treated with Proteinase K (20 μg/mL) for 15 minutes for antigen retrieval and blocked with DAKO background reducing protein blocking agent (Agilent, X090930-2) prior to 1 hour incubation of CD206 (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 μm FFPE tissue sections were cut followed by an antigen retrieval by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies, Germany). Slides were incubated with a monoclonal anti-γH2A.X (phosphor-S139 ...
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bioRxiv - Neuroscience 2021Quote: ... or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005, Dako-Agilent) for 20 min at 97°C using a PT Link (Dako – Agilent) ...