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Citations for Agilent :
3901 - 3950 of 7361 citations for Chick ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Mutated ARNT constructs were generated in the pReceiver-LV105 vector using the Quickchange kit (Agilent) with primers designed to introduce a single S77D mutation ...
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bioRxiv - Cell Biology 2024Quote: ... using the DNF-474 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies Inc., CA, USA) and quantified on the Qubit 2.0 with the Qubit dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Broad Range kit and RNA quality was checked the High Sensitivity RNA ScreenTape Analysis (Agilent). Libraries were generated with the QIAseq miRNA Library Kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA quality was assessed using the DNF-471 RNA kit on a fragment analyzer (Agilent). RNA sequencing libraries were prepared according to the Smart-seq2 protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries’ size distribution and concentration were analyzed respectively by Bioanalyzer DNA High Sensitivity Kit (Agilent) and Qubit (Qubit dsDNA High Sensitivity Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA libraries were generated using the Agilent SureSelect Human All Exon V6 kit (Agilent Technologies). Sequencing was performed using the Illumina NovaSeq platform ...
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bioRxiv - Biophysics 2024Quote: ... served as templates for introducing point mutations using the QuikChange II XL kit (Agilent Technologies). All constructs were confirmed by automated sequencing (LGC Genomics) ...
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bioRxiv - Cell Biology 2024Quote: ... using the DNF-474 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies Inc., CA, USA). The final library is diluted to 1,5 nM prior to denaturation for analysis on a NovaSeq S1 100 cycles run (Illumina Inc. ...
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bioRxiv - Bioengineering 2024Quote: ... The RNA integrity was tested on the Agilent 2100 Bioanalyzer using smallRNA kits (Agilent Technologies). RNA concentrations were calculated by using the Bioanalyzer software ...
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bioRxiv - Genetics 2024Quote: ... This was achieved by means of the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) using primers 5’-CTCAGTGTTTTTCGGACTTGCCGCATGC-3’ and 5’-GCATGCGGCAAGTCCGAAAAACACTGAG-3’ in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The hybrid capture procedure was conducted using the SureSelect XT HS2 DNA Reagent Kit (Agilent) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNA Nano 6000 Assay Kit with the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), respectively ...
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bioRxiv - Immunology 2024Quote: ... The quality of the samples was assessed using a BioAnalyzer RNA Nano kit (Agilent Technologies). RNA libraries were built using an Illumina Stranded mRNA library Preparation Kit (Illumina ...
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bioRxiv - Genomics 2024Quote: ... Site-directed mutagenesis was performed using QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... RNA integrity and concentration were then determined by RNA 6000 Pico Kit on Bioanalyzer (Agilent). RNA were then normalized and synthesied into cDNA using iScript cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and quality checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent) and Qubit dsDNA HS Assay kit (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... an error-prone PCR library were generated using GeneMorph II Random Mutagenesis Kit (Agilent Technologies) and cloned into a plasmid expressing a tripartite fusion protein that includes barnase ...
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bioRxiv - Neuroscience 2024Quote: ... and the Agilent RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA, Cat#5067-1513). RNA-Seq libraries were prepared using the TruSeq mRNA Library Prep Kit (Illumina ...
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bioRxiv - Neuroscience 2019Quote: ... with 0.3% TritonX-100 for 1 hour at room temperature and then incubated overnight at 4°C with the primary antibodies rabbit anti-Iba-1 (Wako: 019-19741, RRID:AB_839504) at 1:500 or rabbit anti-GFAP (Dako: Z0334, RRID: AB_10013382) at 1:1000 and then secondary antibodies (Alexa goat-647 anti-Rabbit ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour incubation with secondary antibodies diluted in 2.5% milk in TBST (1:5000, goat anti-rabbit HRP, or goat anti-mouse HRP, DAKO, #P0448 & #P0447). Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo ...
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were added for a 1-hour incubation at room temperature: anti-mouse IgG HRP-linked (Dako, p0447) and anti-rabbit IgG HRP-linked (Cell signaling ...
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bioRxiv - Cell Biology 2020Quote: ... VWF in supernatant was detected by Western blot with a rabbit polyclonal antibody against VWF ((Dako Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: ... VWF in supernatant was detected by Western blot with a rabbit polyclonal antibody against VWF ((Dako Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with antibody polyclonal Guinea Pig anti-Human Insulin (cat. A21435 - Agilent Technologies, Santa Clara, CA, USA) diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then incubated overnight at 4°C with primary polyclonal anti-GFAP rabbit antibody (1:1000; Z0334, DAKO, Golstrup, Denmark) or anti-collagen IV rabbit antibody (1:200 ...
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bioRxiv - Immunology 2022Quote: ... tissues fixed for 24-30 hours in 10% neutral-buffered formalin (NBF; 3.7% formaldehyde) as previously described (Wiarda et al., 2020) with polyclonal rabbit α-human CD3ε antibody (Dako A0452) and polyclonal goat α-rabbit horseradish peroxidase (HRP)-conjugated antibody (Dako K4003) ...
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bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
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bioRxiv - Developmental Biology 2019Quote: ... Brain sections that were stained with antibodies that required antigen retrieval were incubated in Dako 1X target retrieval solution (Agilent Dako) or sodium acetate buffer ...
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bioRxiv - Immunology 2019Quote: ... Immuno-affinity purification through Protein-A Sepharose beads covalently bound to W6-32 antibodies was performed in a format of 96-well single-use micro plate with 10 µm polypropylene membranes (SeaHorse Bioscience). The plates were then washed 4 times with 2mL 150 mM NaCl and 20 mM Tris HCl (buffer A) ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated for 1 hour at room temperature with biotinylated goat anti-mouse or goat anti-rabbit IgG antibodies (Dako, 1:500), washed 3 times and incubated with streptavidin Cy3 (Sigma ...
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bioRxiv - Neuroscience 2019Quote: Immunohistochemistry was performed on colon sections as described earlier on brain sections58 using additional antibodies (α-Syn: ab27766, Abcam or L001, Enzo; NeuN: MAB377, Merck; MAP2: ab70218 Abeam; TH: 657012, Merck; GFAP: Z0334, Dako).
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bioRxiv - Cell Biology 2019Quote: ... anti-H4K20me2 The following secondary antibodies were used for western blot experiments: peroxidase-conjugated goat anti-rabbit (P448 DAKO, 1:2000) and goat anti-mouse (P447 DAKO ...
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bioRxiv - Bioengineering 2021Quote: ... Both HSV-tk and col3 used a secondary polymer-HRP anti-rabbit antibody incubation for 1 hr (1:200 Dako P0448). For col2 and endomucin staining the manufacturer’s instructions were followed (Vectastain Elite ABC kit ...
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bioRxiv - Bioengineering 2020Quote: ... the sections were incubated with the secondary antibody (goat anti mouse IgG HRP, DAKO P0447, 1:100, EnVision+; System-HRP anti-rabbit DAKO, K4003) for 60 min at RT and stained using DAB peroxidase substrate solution (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... Pelleted cells by the centrifugation for 3 min at 300 rcf are stained with FITC-conjugated anti-lysozyme antibody (Dako, F0372) and APC-conjugated anti-CD24 antibody (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... and were then incubated in a solution containing mouse antibody anti-Aβ (clone 6F/3D; 1:50, Dako M0872, Glostrup, Denmark). The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibodies used were as follows: HRP-conjugated goat anti-mouse (1:5,000 dilution, Dako Agilent, Santa Clara, CA, USA), HRP-conjugated rabbit anti-hamster (1:2000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibodies used were as follows: HRP-conjugated goat anti-mouse (1:5,000 dilution, Dako Agilent, Santa Clara, CA, USA), HRP-conjugated rabbit anti-hamster (1:2000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... for 2hrs at 40°C and then incubated over-night at 4°C on a rotating wheel with 2 μg of anti HBc antibody (Dako B0586). Immune-complexes were captured with protein A/G magnetic beads ...
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bioRxiv - Cell Biology 2021Quote: Paraffin-embedded islet grafts within the kidneys or intact mouse or human islets were stained for insulin using polyclonal guinea pig anti-insulin antibody (Dako, A0564), and apoptosis was measured using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL ...
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bioRxiv - Neuroscience 2021Quote: ... and the membrane was blocked with 5% milk in tris-buffered saline with Tween 20 (TBST) before immunodetection with the following primary antibodies: tau (1:1000, DAKO, Denmark), pTau-S396 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunostaining was performed as described previously(45) with the following antibodies: guinea pig α-insulin (1:2000, Millipore/1:800, DAKO), mouse α-glucagon (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were washed for 3 x 10 min in 1 x Animal-Free Blocker and incubated for 1 h at room temperature in HRP-conjugated rabbit anti-goat secondary antibody (1:1000, Dako, USA) for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM Vanadyl-ribonucleoside complex NEB S1402S) mixed with 1:3000 dilution of probes and 1:900 dilution of guinea pig anti-Insulin antibody (Dako, A0564). Hybridization mix was incubated with tissues for overnight in a 30°C incubator ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 was detected using a rabbit polyclonal anti-hACE2 antibody (Cell Signalling, 4355S; citrate antigen-retrieval) and EnVision+ anti-rabbit HRP (Agilent, K4003). Slides were scanned with a bright field slide scanner (Leica ...
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bioRxiv - Cancer Biology 2020Quote: ... paraffin-embedded human sections were immunolabeled for CD68 and CD163 (details of antibodies are available in Supplementary Table S3) and counterstaining with Mayer’s hematoxylin (cat. #S3309; Dako, Jena, Germany). Immunohistochemical images were analyzed in collaboration with the anatomical pathology laboratory of Hospital North using Calopix software (TRIBVN ...
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bioRxiv - Neuroscience 2022Quote: ... 3x 5 minutes) and incubated in the secondary antibody-horseradish peroxidase (HRP) complex as part of REAL EnVision detection system (Dako #K5007) for 1h at RT ...