Labshake search
Citations for Agilent :
3751 - 3800 of 5601 citations for Rat CD178 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... We measured library fragment sizes on the Agilent Tapestation using the Agilent High Sensitivity DNA 1000 kit (Agilent Technologies) and quantified library concentration using the Qubit IX dsDNA High Sensitivity Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was performed using the streptavidin-biotin-peroxydase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Slides were finally counterstained with haematoxylin ...
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bioRxiv - Immunology 2022Quote: ... The quantity of RNA was determined using Qubit RNA assay kit with Qubit 4.0 and the quality of RNA was tested using agarose gel electrophoresis and High Sensitivity Tape station Kit (Agilent 2200 ...
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bioRxiv - Microbiology 2023Quote: ... extracted RNA quality and concentration were measured using the Bio- analyser RNA Pico kit (Agilent, Cat. No. 5067-1513). Library preparation and sequencing were performed by the Oxford Genomics Centre (https://www.well.ox.ac.uk/ogc/) ...
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bioRxiv - Molecular Biology 2023Quote: All mutagenesis reactions were carried out using the QuikChange II Site-Directed or Lightning MultiSite-Directed Mutagenesis Kits (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The pHD1344tub(PAC)-Nterm3V5-A3 plasmid was mutagenized using the QuickChange Lightning site-directed mutagenesis kit (Agilent, product #210518) and primers listed in Table S2 ...
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bioRxiv - Physiology 2023Quote: ... SIK3-Flag-S884A and S884D plasmids were generated by introducing site-directed mutations into SIK3-Flag plasmid using the QuikChange II XL site-directed mutagenesis kit according to manufacturer’s instruction (Agilent). Lenti-Puro-SIK3-Flag plasmid was constructed by subcloning the “mSIK3-3xFlag” coding sequences (CDS ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... The concentration and quality of RNA samples from day 70 was assessed using a Bioanalyzer RNA kit (Agilent Technologies). Total RNA was prepped with the NuGEN Universal with AnyDeplete Human Ribo kit from ∼100 ng of total RNA input ...
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bioRxiv - Immunology 2023Quote: ... site-directed mutagenesis using specific primers was performed based on the QuikChange site-directed mutagenesis Kit II (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: EEF1A2 mutations in both the CMV-NGFP-EEF1A2-Neo vector and the pcDNA3.1-CMV-Flag-EEF1A2 vector were generated using the QuikChange II site directed mutagenesis kit (Agilent) according to the manufacturers protocol using the following primers.
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bioRxiv - Cell Biology 2023Quote: ... Mutations were inserted by site-directed mutagenesis using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA libraries were constructed using TruePrep DNA Library Prep Kit V2 for Illumina (TD503-02, Vazyme) following the manufacturer’s instructions and quantified by Agilent Bioanalyzer 2100 system ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 TPS was determined using the Dako PD-L1 IHC 22C3 pharmDx kit (Agilent Technologies, Santa Clara, CA), which is approved by the US Food and Drug Administration.
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were validated using DNA Kit on the Agilent 5600 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5600 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Genomics 2023Quote: ... Fragment size was controlled by capillary electrophoresis with the DNA high sensitivity kit and a Bioanalyzer 2100 (Agilent Technologies). DNA ends were repaired using the End-It DNA End-Repair Kit (Epicentre) ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in the secondary antibody-horseradish peroxidase (HRP) complex from the REAL EnVision detection kit (Dako #K5007) for 1h at RT ...
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bioRxiv - Pathology 2023Quote: ... three clusters of lysine- and arginine-residues and one cluster of asparagine residues were exchanged for alanine within mouse Prnp in a pcDNA3.1 vector by site-directed mutagenesis (QuikChange site-directed mutagenesis kit; Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting PCR products were subcloned into the holding vector pSC-B (StrataClone Blunt PCR Cloning Kit, Agilent Technologies) and 16 colonies (white ...
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bioRxiv - Biochemistry 2023Quote: ... For generation of single point LIR mutants and ΔLIR BAG3 the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518) was utilized according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Concentration and quality of the synthesised cDNA were assessed using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627). cDNA was successfully amplified from the suspension containing 8,000 nuclei however cDNA prepared from the suspension containing 8,000 cells was undetectable and therefore library preparation was discontinued at this step ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.2 kb) 20 was labelled by [a-32P]-dCTP by Prime-It II Random Primer Labeling Kit (Agilent Technologies) and hybridized with Hybond-N ...
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bioRxiv - Cell Biology 2023Quote: Single point mutations were inserted in the human RAB6A sequence at position 156 using the QuickChange mutagenesis kit (Agilent).
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bioRxiv - Immunology 2023Quote: ... SAMD9L-F886Lfs*11-E198A/D243A were generated from pMAX:mTFP1-SAMD9L plasmid using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... λgt10 phage vectors in genomic DNA were excised using commercial packaging extract kits (Agilent Technology, Santa Clara, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and fragment size distribution with the Agilent® High Sensitivity DNA Kit (Agilent Technologies, Inc., Cat. No. 5067-4626). Libraries were sequenced as paired-end 150×150bp on a NovaSeq® 6000 System (Illumina ...
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bioRxiv - Genomics 2023Quote: ... and quality was determined with a DNA ScreenTape Kit on the TapeStation 2200 system (Agilent; 5067-5582, 5067-5583). Sequencing was done using a HiSeq 2500 (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies). Individual libraries were diluted to 4nM and pooled for sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplicons were further cloned into a pSC-amp/kan vector using StrataClone PCR cloning kit (240205, Agilent Technologies). We picked ∼20-50 single colonies per sample to determine the presence ...
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bioRxiv - Genomics 2023Quote: ... and size distribution was determined using the high sensitivity DNA kit on the Bioanalyzer 2100 (Agilent, Santa Clara, CA). Pooled libraries were sequenced on the Illumina NovaSeq (paired-end 150 ...
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bioRxiv - Biochemistry 2022Quote: AtCOSY mutants were generated according to the protocol described in the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) using plasmid pHis8-4b::AtCOSY as the template and the primer sequences in Supplementary Table 5 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA quality was assessed using an Agilent 2100 bio-analyzer and the RNA 6000 pico kit (Agilent 5067-1513). Next ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The quantity and quality of extracted RNA samples were analyzed using RNA 6000 Nano kit on a bioanalyzer (Agilent). RNA samples with RNA integrity number (RIN)>7 were used for library construction using the 3′ mRNA-Seq Library Prep Kit FWD for Illumina (QuantSeq-LEXOGEN ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA) and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB ...
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bioRxiv - Physiology 2023Quote: ... followed by Vectastain Elite ABC HRP detection kit (Vector PK-6100) and detection with DAB (Agilent Technologies K346811-2). Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The TEAD-binding defective HPV8 E6 K136N mutant was made using the QuikChange II site-directed mutagenesis kit (Agilent). Nuclear/cytoplasmic fractionation was performed using the previously published Rapid ...
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bioRxiv - Biochemistry 2023Quote: ... The C121S ΔPTPN1 mutant was generated from the ΔPTPN1 vector using a QuikChange II Site Directed Mutagenesis kit (Agilent), and the mutant sequence verified by whole plasmid sequencing (Azenta) ...
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bioRxiv - Biochemistry 2023Quote: ... Library quality was assessed using an Agilent Bioanalyzer 2100 and the Agilent-1000 DNA kit (Agilent Cat# 5067-1504), and libraries were quantified using the Qubit dsDNA Quantitation ...
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bioRxiv - Biophysics 2023Quote: ... 104 and 140) cDNA in pET-7 vector was modified by site directed mutagenesis (QuikChange Lightning kit; Agilent Technologies) to introduce one of four single cysteine mutants (T26C ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA integrity was assessed using a microcapillary electrophoresis (TapeStation 4200, G2991BA) with RNA ScreenTape kit (Agilent Technologies, #5067-5576).
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bioRxiv - Cancer Biology 2023Quote: The mitochondrial function of MAFs was measured with a Seahorse XF Cell Mito Stress Test Kit (103015-100, Agilent) using a Seahorse XF96 Extracellular Flux Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: Site directed mutagenesis was performed on the pEGF-N2-DHRS7 vector according to the manufacturer’s recommendations using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) with primers designed using the Agilent on-line tool (https://www.agilent.com/store/primerDesignProgram.jsp).
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bioRxiv - Neuroscience 2023Quote: ... Quantification and quality checked of libraries was done using an Bioanalyzer 2100 instrument and DNA 7500 kit (Agilent Technologies). Libraries were pooled and sequenced in two lanes on a HiSeq 2500 ...
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bioRxiv - Cell Biology 2023Quote: ... The α10-N839Q mutation was generated by QuickChange mutagenesis kit following the instruction (cat# 200517-5, Agilent Technologies, Inc.). All DNA constructs were identified by sanger sequencing service provided by Retrogen ...
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bioRxiv - Genetics 2023Quote: ... Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518, Agilent Technologies), according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: Introduction of specific mutations in the SOSIP Envs was performed using QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) according to manufacturer’s instructions and verified by sequencing analysis (Eton Bioscience ...