Labshake search
Citations for Agilent :
3701 - 3750 of 5601 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Total RNA quantity and purity were analyzed of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN > 7.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... the cdc14hm mutation (encoding 414QPRK➔414AARA substitutions) was generated with the QuikChange Lightning site-directed mutagenesis kit (210518, Agilent) using pJC347 as the template and confirmed by DNA sequencing ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Mpro mutants were generated with QuikChange® II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-Mpro as the template ...
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bioRxiv - Cell Biology 2022Quote: ... To investigate mitochondrial respiration and energetic phenotypes a Seahorse XFp Cell Mito Stress Test kit (103010-100, Agilent Technologies) was used ...
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bioRxiv - Biochemistry 2024Quote: ... Variants were introduced into the ENTRY construct by site-directed mutagenesis using the QuikChange(TM) II XL kit (Agilent). For the expression of the RocCOR construct in bacteria ...
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bioRxiv - Biophysics 2024Quote: ... Mutagenesis of nCPEB4 phosphorylation sites was performed using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, 210513), with oligonucleotides purchased from Sigma-Aldrich ...
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bioRxiv - Systems Biology 2024Quote: ... Mutation of the miRNA seed-binding sites was performed using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) (Supplementary Figure 2) ...
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bioRxiv - Immunology 2024Quote: ... RNA quantity and purity analysis was done using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Bioengineering 2024Quote: ... Error-prone PCR reactions were carried out following the protocol provided by GeneMorph II Random Mutagenesis Kit (200550, Agilent). Primers were designed to flank residue 1 to 119 of the binder ...
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bioRxiv - Genetics 2024Quote: ... Quality control for the libraries was performed using the Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626). Libraries were sequenced using the Illumina Novaseq 6000 system with 2 × 150 paired-end kits ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to remove genomic DNA contamination before cDNA was synthesised using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent) using Oligo DT ...
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bioRxiv - Genomics 2023Quote: ... and its quality was assessed using the Agilent Fragment analyzer 5200 with the DNA (50kb) kit (Agilent, DNF-467). Next ...
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bioRxiv - Biophysics 2024Quote: ... where residues I3 and I5 were replaced with alanine using the Quick-Change Lightning kit following the manufacturer’s instructions (Agilent). Following mutagenesis ...
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bioRxiv - Cancer Biology 2023Quote: ... Alanine mutagenesis of the Survivin sequence (pGST-Survivin) was carried out using the QuikChangeR Site-Directed Mutagenesis Kit (Stratagene). Plasmid encoding GFP-Survivin D70A/D71A (pGFP-Survivin D70A/D71A ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Oxygen Consumption Rates (OCR) were measured using the Seahorse XF Cell Mito Stress Test Kit (Agilent, Cat. 103708-100) with a Seahorse XFe96 analyzer (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... The CDKN1B Serine 10 to alanine mutant reporter was cloned using the Quikchange II site-directed mutagenesis kit (Agilent).
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bioRxiv - Developmental Biology 2024Quote: ... the slides were treated with the Dako Real Envision Detection System kit (K500711-2, Agilent, Santa Clara, CA, USA) and counterstained with hematoxylin.
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bioRxiv - Immunology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Cyanine 3-labelled cRNA was synthesized from 150 ng RNA using the Low Input Quick Amp Labeling Kit (Agilent) and predicated using RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Neuroscience 2024Quote: ... libraries were visualized on an Agilent 2100 Bioanalyzer using Agilent High Sensitivity DNA kit (Agilent Technologies, Cat. # 5067-4626) and quantified using Qubit dsDNA HS DNA Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Point mutations of Tmem120a were generated using the QuikChange II XL Site Directed Mutagenesis Kit (Agilent, Cat. Nu. 200522) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Fragment traces were determined with a 2100 Bioanalyzer using the Bioanalyzer High Sensitivity DNA kit (Agilent, cat.no.:5067-4626), concentrations were measured using a Qubit dsDNA HS Assay Kit (Thermofisher ...
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bioRxiv - Pathology 2023Quote: ... then washed in PBS and exposed to the detection kit (EnVision + System-HRP Anti-Rabbit, Dako Denmark A/S) which included 3,3′-diaminobenzidine tetrahydrochloride as chromogen and counterstained with Harris hematoxylin ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The concentration and quality of the isolated RNA was checked on an Agilent Bioanalyzer (Agilent RNA 6000 Nano Kit) at the Beijing Genomics Institute (BGI) ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA (260/280>1.8) was extracted using TRIZOL and converted to cDNA using the SensiFast cDNA synthesis kit (Agilent). qRT-PCR was performed against target genes and quantified using a CFX96 Real-Time System (Bio-Rad) ...
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bioRxiv - Genetics 2024Quote: ... the E171Q variant was introduced into an SCN5A expression plasmid (pCDNA3.1) using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent). HEK293T cells were grown at pH 7.4 in filtered sterile DMEM nutrient medium (Life Technologies) ...
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bioRxiv - Genomics 2024Quote: ... and the cfDNA quality was assessed by the Fragment Analyzer High Sensitivity DNA Kit (Agilent, Santa Clara, California, USA).
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bioRxiv - Cell Biology 2024Quote: ... To investigate mitochondrial respiration and energetic phenotypes a Seahorse XFp Cell Mito Stress Test kit (103010-100, Agilent Technologies) was used.
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bioRxiv - Cell Biology 2024Quote: ... a high-quality library was constructed and its integrity was verified using the DNA 1000 assay Kit from Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... Library concentration and fragment sizes were evaluated with the Agilent High Sensitivity DNA kit (Cat. No. 5067-4626; Agilent). Libraries were sequenced on an Illumina NovaSeq 6000 sequencer (2x150 pb ...
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bioRxiv - Microbiology 2024Quote: ... R80A and H71R) or pLRGatewayIRESeYFP-Vpr plasmids were synthesised using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions and primers listed in Table 2 ...
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bioRxiv - Neuroscience 2023Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and Bioanalyzer Agilent 2100 using a High Sensitivity DNA chip (Agilent Genomics). Indexed libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Genetics 2023Quote: ... Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518, Agilent Technologies), according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... and its quality was spot-checked for random samples using the Bioanalyzer High Sensitivity RNA Kit (Agilent 5067-1513). 100 ng of total RNA was quantified using the Qubit RNA High Sensitivity Assay (ThermoFisher Q32852 ...
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bioRxiv - Immunology 2023Quote: Introduction of specific mutations in the SOSIP Envs was performed using QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) according to manufacturer’s instructions and verified by sequencing analysis (Eton Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was performed using the streptavidin-biotin-peroxydase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Slides were finally counterstained with haematoxylin ...
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bioRxiv - Immunology 2022Quote: ... The quantity of RNA was determined using Qubit RNA assay kit with Qubit 4.0 and the quality of RNA was tested using agarose gel electrophoresis and High Sensitivity Tape station Kit (Agilent 2200 ...
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bioRxiv - Microbiology 2023Quote: ... extracted RNA quality and concentration were measured using the Bio- analyser RNA Pico kit (Agilent, Cat. No. 5067-1513). Library preparation and sequencing were performed by the Oxford Genomics Centre (https://www.well.ox.ac.uk/ogc/) ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Molecular Biology 2023Quote: ... megaprimers were synthesized and cloned into the TFAM-mScarlet_pWPXL plasmid following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid containing an IDE E111Q mutant was constructed using a QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) and the pProEx-IDE-wt plasmid ...
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bioRxiv - Genetics 2023Quote: ... We measured library fragment sizes on the Agilent Tapestation using the Agilent High Sensitivity DNA 1000 kit (Agilent Technologies) and quantified library concentration using the Qubit IX dsDNA High Sensitivity Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Biophysics 2023Quote: ... 104 and 140) cDNA in pET-7 vector was modified by site directed mutagenesis (QuikChange Lightning kit; Agilent Technologies) to introduce one of four single cysteine mutants (T26C ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA integrity was assessed using a microcapillary electrophoresis (TapeStation 4200, G2991BA) with RNA ScreenTape kit (Agilent Technologies, #5067-5576).
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bioRxiv - Physiology 2023Quote: ... SIK3-Flag-S884A and S884D plasmids were generated by introducing site-directed mutations into SIK3-Flag plasmid using the QuikChange II XL site-directed mutagenesis kit according to manufacturer’s instruction (Agilent). Lenti-Puro-SIK3-Flag plasmid was constructed by subcloning the “mSIK3-3xFlag” coding sequences (CDS ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...