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Citations for Agilent :
3701 - 3750 of 4652 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products ...
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bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies were followed by respective HRP-conjugated anti-mouse or anti-rabbit secondary antibody (1: 2000; Dako). Detection of peroxidase activity was performed by using the Amersham ECL western blot detection kit (GE Healthcare).
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bioRxiv - Genetics 2022Quote: ... followed by incubation with goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP) (1:2000, Agilent Cat# P0448, RRID:AB_2617138). Ubiquitin was detected using mouse monoclonal anti-ubiquitin antibody (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... retinal cross-sections were treated with antigen retrieval solution at 99 °C for 1 h (PH 6.1; Dako #S1699) and washed in 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used were listed as follows: rabbit anti-GFAP (a marker for astrocytes, 1:1000, Cat# Z0334, DAKO), rabbit anti-NeuN (a marker for neurons 1:1000 ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation variants found in P.1 and 10-mutation variant (BZΔ10) were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana VSV (rVSV ...
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bioRxiv - Molecular Biology 2020Quote: ... XL10-Gold ultracompetent cells (Tetr Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F’proAB lacIqZΔM15 Tn10 (Tetr) Amy Camr] (Agilent Technologies) were used.
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bioRxiv - Neuroscience 2020Quote: ... The nuclei were stained with DAPI (1:500 in 0.1M PBS) and mounted on glass slides using fluorescent mounting media (DAKO) and examined in a fluorescence Observer Z1 microscope with ApoTome 2 under a Plan-Apochromat 40x/1.3 Oil DIC objective (Carl Zeiss) ...
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bioRxiv - Biochemistry 2019Quote: ... 1 µl sample was injected in splitless mode with an Agilent 7693 autosampler injector into deactivated splitless liner (Agilent 5190-3167 ...
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Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetesbioRxiv - Cell Biology 2019Quote: ... Pancreas tissue sections (8 um) were stained using the following primary antibodies: guinea pig anti-insulin (DAKO; 1:500), goat anti-pancreatic polypeptide (abcam ...
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bioRxiv - Microbiology 2020Quote: ... The sample (1 μL) was injected onto a VF5-MS column (30 m × 0.25 mm × 0.25 μm; Agilent Technologies) with an inlet temperature of 280 °C and a split ratio of 20:1 ...
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bioRxiv - Pathology 2021Quote: ... The slides were treated with 1× Dako Peroxidase-Blocking Solution® (cat S2023; Agilent Technologies Inc., Santa Clara, CA) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were probed with appropriate primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1/5000 (Dako Agilent). Proteins were detected with ECL Western Blotting Substrate (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were probed with appropriate primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1/5000 (Dako Agilent). Proteins were detected with ECL Western Blotting Substrate (Promega ...
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bioRxiv - Cancer Biology 2019Quote: ... the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies). The enzymatic reaction was conducted with 1-Step Ultra TMB-ELISA (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... samples were passed through an immobilized pepsin column (2mm × 2cm) at 50μl min-1 and the digested peptides were captured on a 2mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was transferred to nitrocellulose membrane for 1 hour via vacuum transfer as described in Stratagene’s Membranes Instruction Manual (Stratagene) and crosslinked using a Stratalinker UC Crosslinker (Stratagene) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μl of each RNA extract was used for testing RNA quality and quantity using a Bioanalyzer Systems (Agilent) at the LUMC hospital in Leiden (the Netherlands ...
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bioRxiv - Neuroscience 2021Quote: ... Heat-based antigen retrieval was performed using 1× antigen retrieval solution at pH 9 (Agilent Technologies; Santa Clara, CA) for 1 hour (30 min at 95C ...
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bioRxiv - Immunology 2020Quote: ... A total of 1 × 106 PBMC was seeded in 24-well XF24 cell culture microplates (Agilent Technologies, Waldbronn, Germany), while four wells were kept free from cells as background correction ...
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bioRxiv - Immunology 2021Quote: ... and a 1:10 dilution of the resulting cDNA was run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess cDNA quality and yield ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated for 1hr at room temperature with polyclonal HRP-conjugated goat anti-rabbit (1:5000; Dako, Denmark) or anti-mouse (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were then incubated with anti-PD-L2 (CST #82723) diluted 1:25 in EnVision FLEX antibody diluent (Dako) over night at 4º C ...
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bioRxiv - Biochemistry 2022Quote: ... The column exit was connected to the ESI source of the mass spectrometer using PEEK tubing (0.005” inner diameter × 1/16” outer diameter, Agilent). Mass spectra were acquired in the positive ion mode over the range m/z = 350 to 1800 using the Orbitrap mass analyzer ...
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bioRxiv - Cell Biology 2022Quote: ... the cell nuclei were stained with a 1:2000 dilution of SPY-DNA dye (Spirochrome) in antibody diluent (Dako). SPY-DNA 505 ...
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bioRxiv - Immunology 2022Quote: ... The tetrameric detection reagents were generated immediately prior to use by combining biotinylated FcɣR proteins (Ackerman Laboratory) with a 1/4th molar ratio of Streptavidin-Allophycocyanin (ProZyme) and mixing by inversion for 10 min ...
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bioRxiv - Immunology 2022Quote: ... and a 1:10 dilution of the resulting cDNA was run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess cDNA quality and yield ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... then reconstituted in 1 mL of 0.1 % trifluoroacetic acid and desalted using an Agilent Macroporous Reversed-Phase C18 column (4.6 × 50 mm mRP-C18, Agilent) on a 1260 Infinity HPLC system (Agilent).
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bioRxiv - Neuroscience 2022Quote: ... The slides were washed with 0.1 M Sorensen’s phosphate buffer and the staining was developed with a 1:50 solution of DAB chromogen (K3468, Dako) and 0.005% hydrogen peroxide in Sorensen’s phosphate buffer for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The staining was developed using a 1:50 dilution of 3,3’-diaminobenzidine (DAB) chromogen in DAB+ substrate buffer (GV825, Dako) for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... CFSs were diluted in SDW (ratio 1/20) and injected into a high-performance liquid chromatography (HPLC) system (Agilent 1100 Series 203 HPLC System ...
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bioRxiv - Cell Biology 2022Quote: The following secondary antibodies were used for western blot experiments: peroxidase-conjugated goat anti-rabbit (P448, DAKO, 1:1000) and goat anti-mouse (P0447 ...
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bioRxiv - Genomics 2024Quote: ... The quality of nucleic acids was assessed by agarose gel electrophoresis (1% for RNA, 0.7% for DNA) and on-chip electrophoresis (Bioanalyzer 2100, Agilent).
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bioRxiv - Synthetic Biology 2024Quote: ... and an Agilent 7693A autosampler fitted with a 100 μL gastight syringe was used to inject headspace gas (50 μL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 μm film; Agilent). Data analysis was performed using Agilent MassHunter WorkStation Quantitative Analysis ...
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bioRxiv - Synthetic Biology 2024Quote: ... and an Agilent 7693A autosampler fitted with a 100 μL gastight syringe was used to inject headspace gas (50 μL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 μm film; Agilent), and (iii ...
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bioRxiv - Cell Biology 2024Quote: ... GC-FID was performed using an Agilent 7890 gas chromatograph equipped with a DB-WAX column (15 m x 0.53 mm, 1 µm; Agilent) and flame ionization detection ...
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bioRxiv - Synthetic Biology 2023Quote: ... An Agilent 7693A autosampler fitted with a 100 µL gastight syringe was used to inject headspace gas (50 µL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 µm film; Agilent). Experiments were performed using a 55:1 split ratio ...
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bioRxiv - Microbiology 2023Quote: ... RNA integrity was assessed with 1% (m/v) agarose gel and Agilent 2100 Bioanalyzer System (Agilent Technologies, 5067-4626). For antibiotic treatment conditions ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Developmental Biology 2023Quote: ... the beads were transferred into a 1-mL PP filtration microplate (Agilent, 7 µm frit, cat. no. 202501-100) and washed 3x with 200 µL 1x PBS and 2x 200 µL ultrapure water ...
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bioRxiv - Biophysics 2023Quote: Drp1 isoform 1 (Genbank accession number AB006965) was PCR amplified with Pfu Turbo DNA polymerase (Stratagene, La Jolla, CA) as an NdeI/XhoI fragment ...
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bioRxiv - Biochemistry 2023Quote: ... and m6A(1-100 mM, MCE HY-N0086) were analyzed on RapidFire 300 high-throughput SPE system (Agilent Technologies) interfaced with a Sciex 6500 (Sciex) ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary anti-mouse-HRP (Santacruz sc-516102 Lot#c1419) was diluted 1: 10,000 or anti-rabbit-HRP (Dako P0448) 1:1000 in blocking buffer and incubated for 1hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... RNA quality was measured by 1% agarose gel electrophoresis (Figure S3A) and by High Sensitivity (HS) DNA Assay (Agilent) (Figure S3B).
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were also assessed for size distribution and adapter dimer presence (<0.5%) by the High Sensitivity NGS Fragment 1-6000bp kit on a 96-channel Fragment Analyzer (Agilent). All sequencing libraries were then normalized on the MicroLab STAR (Hamilton) ...
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bioRxiv - Cell Biology 2024Quote: ... and qualitatively assessed with the High Sensitivity NGS Fragment 1-6000bp Kit on a 96-channel Fragment Analyzer (Agilent). Final libraries were normalized ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed in PBS-T (0.05% Tween- 20 in PBS) and PBS before incubation with either 1:20,000 dilution of HRP-conjugated rabbit anti-human IgG (Dako) or 1:10,0000 dilution of HRP-conjugated goat anti-mouse IgG (Seracare ...