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Citations for Agilent :
3551 - 3600 of 4015 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... FACS sorted MPs were plated on XF96 cell culture microplates coated with 10% Matrigel in warm assay medium (Agilent), the cell culture plate was centrifuged with 200 g for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Seahorse records were corrected for well-to-well differences in the cell numbers and analyzed using Wave software (Agilent). Leukemia cells (40,000 cells/well for cell lines ...
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bioRxiv - Cancer Biology 2020Quote: ... A549N cells were seeded in normal media in Seahorse xFP plates (Cat# 103022-100, Agilent Technologies, Santa Clara, CA) 24 h prior to conducting the assay ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2×107 HeLa cells were crosslinked on ice with 0.8 J/cm2 UV 254 nm in a UV Stratalinker 2400 (Stratagene), washed in PBS with 2 mM EDTA and 0.2 mM PMSF ...
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bioRxiv - Immunology 2021Quote: Synthesised antigen constructs were transformed into competent XL-1 Blue Escherichia coli cells (Agilent Technologies, Santa Clara, CA, USA) by electroporation ...
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bioRxiv - Cancer Biology 2020Quote: Untargeted metabolomics profiling of PCK1-overexpressing cells was performed using ultra-high-performance liquid chromatography (Agilent 1290 Infinity LC) coupled to with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Subsequently we strictly adhered to the Seahorse XF Cell Mito Stress Test Kit protocol (Agilent Technologies Cat# 103015-100). Cells were counted before and after the assay using a Celigo S Image cytometer (Nexcelom Biosciences ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... at 22.4µg/ml concentration per well to prepare Cell-Tak coated XF96 microplates according to the manufacturers guidelines (Agilent Technologies). Cells were seeded into the coated microplates immediately before the assay by centrifugation with 200g for 1min without brake ...
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bioRxiv - Cell Biology 2020Quote: ... 2 x 104 cells were seeded into each well of a V3 96-well plate (Agilent, Cat. # 101085-004) and cultured 24 h before measuring OCR ...
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bioRxiv - Cell Biology 2020Quote: ... The number of cells per well was directly incorporated into the Wave software (Seahorse Wave Controller 2.4 software, Agilent) to normalize OCR and ECAR values ...
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bioRxiv - Bioengineering 2020Quote: ... VO2max = 1.04×10−16 mol/s/cell was measured for hiPSC-Heps using the Seahorse XF24 cellular respirometer (Agilent) (see ...
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bioRxiv - Cell Biology 2021Quote: Oxygen consumption rates (OCR) from differentiated SVF cells were analyzed by the Seahorse XF24 Extracellular Flux Analyzer - (Agilent Technologies). Cells were seeded ...
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bioRxiv - Cancer Biology 2022Quote: ... and read by brightfield and fluorescence imaging on a BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies) every six hours for a total of six days ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Isolated mouse cardiomyocytes were seeded at 2K per well in the Seahorse XF96 cell culture microplates (Agilent Technologies, 102416100) pre-coated with laminin mouse protein at 2μg/cm2 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were overlaid with 175 μL of mitochondrial assay solution [33] supplemented with the Seahorse Plasma Membrane Permeabilizer (Agilent), 4 mM ADP (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... The myr(–)MA and myr(–)MA99 proteins were overexpressed in Escherichia coli BL21 Codon Plus-RIL cells (Agilent Technologies). Cells were grown in LB broth supplemented with 100 mg/L Ampicillin at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: iNeurons were replated on DIV 4 onto polyornithine/laminin coated in the Seahorse XF96 Cell Culture Microplate (Agilent Technologies) at a density of 50 000/well ...
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bioRxiv - Neuroscience 2019Quote: Mitochondrial respiratory function in live SH-SY5Y cells was measured using the Seahorse XFe24 Extracellular Flux Analyzer (Seahorse Bioscience) via changes in the oxygen consumption rate (OCR ...
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bioRxiv - Immunology 2019Quote: ... harvested after 72 hours of culture and plated on 96-well XF cell culture microplates in XF assay medium (pH 7.4, both from Agilent) supplemented with D-glucose (20mM ...
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bioRxiv - Biochemistry 2021Quote: ... These data were then normalized to the corresponding DNA/protein/cell number in the Wave data analysis software (Agilent) and exported to Microsoft Excel.
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from cryopreserved tumour samples or cultured cells using the Total RNA Isolation Micro kit (Agilent) and cDNA then synthesized using SuperScript VILO cDNA synthesis kit (Life Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... and centrifuged again to remove the supernatant.RNA was then extracted from the isolated disc cells using Absolutely RNA Nanoprep Kit (Stratagene), following manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... the fragments were joined by Gibson assembly and used to transform chemically competent BL21-Gold(DE3) cells (Agilent Technologies). Individual clones were sequence-verified by Sanger sequencing (Microsynth AG) ...
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bioRxiv - Molecular Biology 2019Quote: ... N≥50 worms (10 worms/ well) were transferred to the Seahorse XF96 Cell Culture Microplate (Agilent Technologies, 101085-004) in a total volume of 180 uL ...
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bioRxiv - Cell Biology 2020Quote: Oxygen consumption rates (OCR) were measured in iBAs cells using a Seahorse XFe96 (Agilent Technologies, Santa Clara, CA, USA). Briefly ...
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bioRxiv - Biochemistry 2021Quote: HMECs were cultured at a density of 3 x 104 cells/well in 24-well Seahorse XFe24 plates (Agilent) and incubated at 37 °C under a humidified 5% CO2 atmosphere overnight in the presence or absence of DMF ...
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bioRxiv - Neuroscience 2021Quote: ... shuttle plasmids were co-transfected with the pDF9 helper plasmid into HEK293-AAV cells (Agilent Technologies, Santa Clara, USA). Cells were lysed 72h following transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked in 0.2% triton X-100 solution containing 1% BSA and 5% normal goat serum (DAKO, X0907) for 1 hour.
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bioRxiv - Developmental Biology 2021Quote: ... at a density of 7 x 105 cells per cm2 in Seahorse XF DMEM (Agilent cat. no. 103575-100) supplemented with 10mM glucose (Agilent cat ...
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bioRxiv - Cell Biology 2020Quote: Forty-thousand HUVECs representing each genotype (0RV, 1RV, and 2RV) were seeded on V7 cell culture plates (Seahorse Bioscience). Cells were either left untreated or treated with IFNɣ (50pg/mL ...
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bioRxiv - Microbiology 2020Quote: ... The MUF production (fmol MUF cell-1 h-1) was measured with a Cary Eclipse spectrofluorometer (FL0902M009, Agilent Technologies) at 359 nm (excitation ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2×107 cells were crosslinked on ice with 0.8 J/cm2 UV 254 nm in a UV Stratalinker 2400 (Stratagene), washed in PBS with 2 mM EDTA and 0.2 mM PMSF ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.104 cells were seeded into 96 well plates suitable for a 96-well metabolic analyser (Seahorse XF96, Agilent Technologies). 24 hours later and prior to the assay ...
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bioRxiv - Immunology 2020Quote: ... A total of 1 × 106 PBMC was seeded in 24-well XF24 cell culture microplates (Agilent Technologies, Waldbronn, Germany), while four wells were kept free from cells as background correction ...
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bioRxiv - Immunology 2021Quote: ... After 2h of AIM2 activation cells were processed per manufacturer’s directions and analyzed using the Agilent Seahorse Mito Stress Test kit (Agilent). For over-night stimulation with LPS cells were treated 2h after plating with 10 ng/mL LPS and incubated overnight at 37°C.
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p97/VCP induces GLI1 to control XBP1-dependent endoplasmic reticulum stress transcriptional responsebioRxiv - Cancer Biology 2021Quote: ... Then in the dark (to protect the signal from light) the cells were incubated with 3,3’--diaminobenzidine (DAB, diaminobenzidine, EnVision System, Dako) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... cooled down to room temperature for 20 minutes and used for the transformation of XL1 blue competent cells (Agilent). All identified mutations (Fig ...
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bioRxiv - Genetics 2022Quote: The wild-type and mutant BRD-1-BRC-1 chimeras were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). The cells were grown at 37°C until OD600 0.6 and were induced by 0.2mM isopropyl-β-D-thiogalactoside in the presence of 100μM ZnCl2 at 37°C for 6 hrs ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and mounted on glass slide using mounting media (Dakocytomation Fluorescence Mounting Media, Dako, USA). The cells were examined using either a Nikon Eclipse 80i (Japan ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μM fluoro-carbonyl cyanide phenylhydrazone (FCCP) and 0.5μM/0.5μM rotenone/antimycin A (all XFp Cell Mito Stress Test Kit, Agilent Technologies, 103010-100) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fragments were cloned into the StrataClone TA-cloning vector and transformed into StrataClone SoloPack competent cells according to the manufacturer’s protocol (StrataClone PCR Cloning Kit, Agilent), resulting in pFF-162 (SpoY) ...
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bioRxiv - Cell Biology 2022Quote: ... To investigate mitochondrial respiration and energetic phenotypes a Seahorse XFp Cell Mito Stress Test kit (103010-100, Agilent Technologies) was used ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed once with DMEM Assay Medium (XF DMEM [Agilent] supplemented with XF glucose [10 mM, Agilent] ...
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bioRxiv - Cell Biology 2024Quote: The oxygen consumption ratio (OCR) was determined using Seahorse XF Cell Mito Stress Test (Agilent, Santa Clara, CA, US). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Cellular OCR was measured using a Seahorse XF Cell Mito Stress Test and a Seahorse Bioanalyzer XFe96 (Agilent Technologies) according to the manufacturer’s standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture media was replaced with assay media (Seahorse XF DMEM medium pH 7.4, Cat. No. 103575-100, Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: HAECs were seeded at a density of 5,000 cells/well per on Seahorse XF24 tissue culture plates (Seahorse Bioscience). On the day of experiment ...
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bioRxiv - Neuroscience 2024Quote: RNA samples of sorted Gsh2-lineage cells (eGFP+) from control and cKO embryos (E15.5) were submitted for quality control with the BioAnalyzer (Agilent) at the Genomics Core (KU Leuven) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed three times with PBS and mounted on glass - slides using fluorescent-DAKO mounting media (Agilent Technologies). Images of tubulin morphology and costainings of tubulin and Par3 were captured at the Sanford Burnham Prebys Cell Imaging Core ...
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bioRxiv - Cell Biology 2024Quote: ... To investigate mitochondrial respiration and energetic phenotypes a Seahorse XFp Cell Mito Stress Test kit (103010-100, Agilent Technologies) was used.