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Citations for Agilent :
3551 - 3600 of 3831 citations for Platelet endothelial aggregation receptor 1 PEAR1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Qualitative and quantitative measurement of cDNA was conducted with the High Sensitivity NGS Fragment 1-6000bp kit on a 48-channel Fragment Analyzer (Agilent) and 1x dsDNA kit on the Qubit 4 Fluorometer (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: MBP-MPS1104-330/WT and MPS1104-330/4A were obtained by over-expression in 1 L culture of BL21CodonPlus(DE3)-RIL cells (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... infected with VSVΔM51-GFP at the indicated MOIs and treated with 0.3-3µM CBL0137 (or media alone) 1 hpi using the Agilent Bravo liquid handling system (Agilent, CA, USA). 24 hpi ...
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bioRxiv - Microbiology 2024Quote: ... The amplified vectors were digested with Dpn1 and 1 µL of each (vector and promoter region insert) were transformed into ultracompetent XL-10 Gold cells (Agilent) for in vivo assembly ...
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bioRxiv - Immunology 2024Quote: ... Serum samples were diluted 1:50 in PBS + 25% FBS and data were acquired with a BioTek Synergy H1 plate reader (Agilent). ELISA data were analyzed with GraphPad Prism (Dotmatics ...
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bioRxiv - Immunology 2024Quote: ... The single and triple-site mutations were introduced into the pcDNA3.1 vector encoding SARS-CoV-2 wildtype or BA.1 Spike using QuickChange site-directed mutagenesis (Agilent 210519) according to the instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The antigen retrieval process involved incubating the sections with DAKO Target Antigen Retrieval Solution (diluted 1:10; Agilent Technologies, USA) in a water bath at 90 °C for 45 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... One aliquot was solubilized with 1% SDS to determine protein content and the other aliquot was mixed with 1 mL 30% HNO3 and subjected to ICP/MS 8900 (Agilent). Data was normalized by the corresponding protein concentration.
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bioRxiv - Plant Biology 2024Quote: ... A splitless injection volume of 1 μL was injected onto an HP-5 column with 5% phenyl methylpolysiloxane stationary phase (Agilent) with dimensions of 30 m x 0.25 mm x 0.25 μm ...
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bioRxiv - Biochemistry 2024Quote: The HAdV5HVR7 vectors (“1st generation” vectors) were obtained by cloning the gene of interest into the pAdEasy-1 plasmid system from Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mM Seahorse XF L-glutamine solution and 1 mM Seahorse XF pyruvate solution (103577-100, 103579-100 and 103578-100, all from Agilent) and cells were kept in a CO2 free incubator for 45 min prior to starting the Cell Mito Stress Test ...
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bioRxiv - Molecular Biology 2024Quote: ... which was amplified in 15 PCR cycles using Herculase II Fusion DNA polymerase in a 100-μl reaction mixtures (1x Herculase II reaction buffer, 1 mM dNTPs, 200 pM Agilent library ...
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bioRxiv - Neuroscience 2024Quote: Cells were counted and plated at 1 × 105 (mouse astrocytes or neurons) cells per well in a Seahorse XF24 Cell Culture Microplate for all experiments (Agilent). Cells were then treated with indicated inhibitors or agonists in each experiment for 20 h ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 h before being placed into a Seahorse XFe24 Analyzer (Agilent). For OCR and ECAR mitostress test experiments ...
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were subsequently separated by Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Bands were quantified and PSI was calculated as follows ...
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bioRxiv - Physiology 2024Quote: ... or 32°C (naked mole- rat) and the O2 level was set to 1%O2 using the gas controller (Agilent). After 4 hours of hypoxia ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a DB-5 column (inner diameter, 0.25 mm; length, 30 m; film thickness, 1 μm; #122-5033, Agilent Technologies). Helium was used as carrier gas ...
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bioRxiv - Biophysics 2024Quote: ... a ∼5 V cm-1 electric field was manually applied across the compartment using a DC power supply (E3620A, Agilent) to pull the chromosome into the bottom capillary ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed and re-suspended in HBSS with 2% FBS with 1 μg/ml propidium iodide before acquisition on a Novocyte Quanteon or Penteon (Agilent).
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed 5 times with 1X PBST and incubated with horseradish peroxidase conjugated secondary anti-mouse or anti-rabbit IgG (1:5000, Cat # P0161 and P0448, respectively; DAKO) in 1X PBST for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence was monitored with excitation and emission filters set at 465 and 590 nm during gradual heating (1 K/min) from 25 °C to 95 °C in a MX3005P real-time PCR instrument (Stratagene). Data was evaluated using MXPro3005 software (Stratagene ...
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bioRxiv - Cancer Biology 2024Quote: ... The LZK Q240S drug-resistant construct and K195M kinase-dead mutant (1) was introduced using a Site-Directed Mutagenesis Kit (Stratagene) (Primers listed in Supplementary table S2) ...
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bioRxiv - Cell Biology 2024Quote: ... RPE1 cells (1×104) of the indicated genotypes were seeded in 96-well Seahorse XFe96 cell culture microplates (Agilent Technologies) for 24 h in eight replicate wells ...
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Bioengineering 2021Quote: ... 10-20 µl injection volume depending on protein concentration) were injected on a Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) at a flow rate of 100 µl/min using solvent A (0.1% formic acid and 0.05% trifluoroacetic acid in water ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to synthesize cDNA from 1 ug RNA and Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) was used for the Quantitative RT-PCR reactions ...
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bioRxiv - Microbiology 2020Quote: The quality of purified libraries was analyzed on a Bioanalyzer High Sensitivity DNA chip (Agilent; see Figure 1 for an example) by following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: 3000 control or p107KO cells or p107KO cells 24 hours post transfection with pCMV6-OCT-HA-eGFP alone or together with p107fl or p107mls were seeded in DMEM (Wisent) containing 10% FBS and 1% penicillin streptomycin on microplates (Agilent Technologies) and treated according to the required experiment ...
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bioRxiv - Immunology 2021Quote: Total IgG from individual donors was purified from ∼1 µL plasma using the AssayMAP Bravo platform (Agilent Technologies, Santa Clara, USA) with Protein G-coupled cartridges as described elsewhere (Larsen et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The quality of the libraries is determined using the Standard High sensitivity NGS Fragment analysis kit (DNF-474, 1-6000 base pair) on the Agilent Fragment analyzer (Agilent, USA), yielding approximately 260 bp size fragments ...
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bioRxiv - Plant Biology 2020Quote: ... RT-PCR was performed with 1μL of diluted cDNA (1:9) in a Brillant III ultra fast SYBRgreen QPCR master mix (Agilent Technologies, USA) using a StepOnePlus Real-Time PCR System (Applied biosystems ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and then diluted to 1 ng/uL before checking insert size on an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany) and quantifying to greater accuracy by quantitative Q-PCR (library molarity >2 nM) ...
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bioRxiv - Systems Biology 2021Quote: ... using an Aminex HPX-87H ion-exchange column operated at 60°C with 5 mM H2SO4 as the mobile phase with a flow rate of 0.6 mL min-1 (Agilent, Santa Clara). The OD660 was measured with a Jenway 7200 spectrophotometer (Jenway ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... The extent of shearing was monitored with a 1% agarose gel and confirmed by separation on a 2100 High sensitivity Bioanalyzer chip (Agilent Technologies) upon completion of the immunoprecipitation ...
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bioRxiv - Biochemistry 2020Quote: ... The combined organic extract was concentrated to ~1 mL prior to clean-up by SPE (Agilent Bond Elut SI, 500 mg). The SPE columns were conditioned with hexane and the extract eluted with 6 mL 10% dichloromethane in hexane ...
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bioRxiv - Neuroscience 2020Quote: ... and then diluted to 1 ng/µl before checking insert size on an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany) and quantifying to greater accuracy by quantitative Q-PCR (library molarity >2 nM) ...
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bioRxiv - Molecular Biology 2022Quote: ... we created pcDNA3-AhR-1(LBD)-VP16 by site-directed mutagenesis of the pcDNA3-AhR-1-VP16 plasmid with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523). The following primer pair was used to create an L to A substitution at L363 ...
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bioRxiv - Neuroscience 2022Quote: ... Plasma (50 μL) was diluted by adding 150 μL of Agilent buffer A (1:4 dilutions, as recommended by Agilent Technologies), then filtered using a 0.45μm spin filter (Spin-X centrifuge tube filter ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Arrays were then washed with probing buffer (0.1% Tween 20, 1% BSA, 10% PBS) and incubated for 1.5 h in a hybridization chamber (Agilent, Santa Clara, CA) in a reaction mixture containing 80 μg of purified His-SETD6 in probing buffer in a total reaction volume of 950 μl ...
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bioRxiv - Plant Biology 2021Quote: ... samples by using modified CTAB and LiCl method [24,25] The quality of isolated RNA measured by UV spectrophotometer at 260/280and 260/230 nm wavelengths and 1% agarose gel electrophoresis followed by measuring RNA concentration using a 2100 Bioanalyzer (Agilent Technologies). The concentration of RNA was obtained in SaSHc 1460.90 ng/μl and in SaSLc 12.65 ng/μl ...
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bioRxiv - Molecular Biology 2021Quote: ... approximately 8 µg of protein was injected on a Zorbax 300SB-C18 column (5 µm, 300Å, 1×250mm IDxL; Agilent Technologies) and separated using a 30 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Cancer Biology 2021Quote: ... W503F (PBD, polo-box domain 1) and H629A, K631M (PBD, polo-box domain 2) were generated by site-directed mutagenesis (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The PmeI-linearized pShuttle-vectors carrying different cDNA fragments were introduced into the replication-deficient adenoviral vector pAdEasy-1 through homologous recombination in BJ5183 cells (Agilent Technologies). Successfully recombined adenoviral vector was verified by sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... Extracts of the cross-linked pellets were processed into sequencing libraries as previously described (Bohnsack, Tollervey, and Granneman 2012) using 1µl of a 1:100 dilution of 10U/µl RNace-IT (Agilent Technologies, 400720) per sample ...
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bioRxiv - Genetics 2021Quote: ... and the quality of the sample was checked by running a 1% agarose gel and by RNA 6000 Nano assay on a 2100 Bioanalyzer (Agilent Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... the slides were treated with 1:10 normal goat serum in PBS for 15 minutes and incubated for 60 minutes with 1:200 diluted Rabbit anti-human thyroglobulin (Dako, Denmark) or 1:400 diluted Rabbit anti-human calcitonin (Dako ...
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bioRxiv - Cancer Biology 2021Quote: WT and CCN4-KO melanoma cells were cultured overnight (1 × 104 cells/well) in Seahorse XFe96 cell culture microplates (Agilent, CA) with complete DMEM medium ...
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bioRxiv - Cell Biology 2020Quote: ... The Samples were diluted 4 fold with water and 1 μl supernatant was loaded on a Bioanalyzer High Sensitivity DNA electrophoresis chip (Agilent Technologies).