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Citations for Agilent :
3551 - 3600 of 5977 citations for Human CD283 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Mutagenesis was carried out with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Cat # 210518-5). Mutant gerAB sequences were integrated into the original gerAB locus by a double crossover along with an erythromycin (erm ...
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bioRxiv - Molecular Biology 2024Quote: ... and the quality was evaluated with an Agilent Bioanalyzer system (RNA 600 Nano kit – Agilent, Waldbronn, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... site-directed mutagenesis (SDM) was performed using a QuikChange II Site-Directed Mutagenesis Kit (Agilent, Santa Clara, US). Primer sequences for SDM are included in Table S9.
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bioRxiv - Plant Biology 2020Quote: LiAc yeast transformation was performed as described in the GAL4 Two-Hybrid Phagemid Vector Kits manual (Agilent Technologies). The constructions including either DNA binding domain or activation domain were co-transformed into yeast strain PJ69-4a ...
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bioRxiv - Biophysics 2021Quote: ... Point mutants were engineered into the DHFR gene using QuikChange II site-directed mutagenesis kits (Agilent cat#200523) using primers specified in Appendix 1 ...
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bioRxiv - Biophysics 2021Quote: ... R214Q/K219N/R353Q triple mutation) were generate using the Quik Change II XL Site-Directed Mutagenesis Kit (Agilent) and verified by sequencing the entire cDNA construct ...
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bioRxiv - Biophysics 2021Quote: The error-prone PCR reaction for libraries “SH3_EP” was done using the GeneMorph II Mutagenesis Kit (Agilent Technologies) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA integrity was assessed using Agilent RNA 6000 Nano kit and 2100 Bioanalyzer instrument (both from Agilent Technologies). Total RNA was depleted from ribosomal RNA using the Ribo-Zero Depletion Kit for Gram-negative bacteria (Illumina ...
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bioRxiv - Biophysics 2020Quote: ... Site directed mutagenesis was performed with the help of Quick-change Site-Directed Mutagenesis kit (Stratagene, Agilent Technologies) to generate several single tryptophan (W ...
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bioRxiv - Biophysics 2020Quote: ... Site directed mutagenesis was performed with the help of Quick-change Site-Directed Mutagenesis kit (Stratagene, Agilent Technologies) to generate several single tryptophan (W ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA quality was confirmed on the Agilent Bioanalyzer 2100 using the RNA 6000 Nano Kit (Agilent, 5067-1511). Total RNA-seq libraries were generated using NEBNext Poly(A ...
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bioRxiv - Cell Biology 2020Quote: ... The PICK1 missense mutations were introduced into the KD + WT FUGW vector using the QuickChange® kit (Stratagene). The pHSynXW vector encoding eGFP-rPICK1 was a gift from Prof ...
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bioRxiv - Genetics 2021Quote: ... The total RNA was amplified using the Sure Select Stranded RNA-Seq kit (Agilent Technologies; Santa Clara, CA) using standard protocols ...
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bioRxiv - Genetics 2021Quote: ... cloned into an expression plasmid driven by CMV promoter and modified using QuikChange Site-Directed Mutagenesis Kit (Agilent). For each affinity purification ...
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bioRxiv - Developmental Biology 2021Quote: ... and Cy3-labeled complementary RNAs (cRNAs) were synthesized using the Low Input Quick Amp Labeling Kit (Agilent Technologies). The cRNAs were hybridized on a SurePrint G3 Mouse Gene Expression 8 × 60K Microarray (Agilent Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was purified from ∼200 motor neurons per biological replicate using the Absolutely RNA Nanoprep Kit (Agilent). Amplified cDNA was prepared from total RNA using the Ovation PicoSL WTA System V2 Kit (NuGEN ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA quality was evaluated using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Nano Kit (Agilent Technologies, 5067-1511). Libraries were prepared using an Illumina® TruSeq® Stranded Total RNA kit with ribosomal depletion by Ribo-Zero Gold solution from Illumina® according to the manufacturer’s protocol and sequenced on an Illumina® HiSeq 4000 (2x 75 bp).
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bioRxiv - Molecular Biology 2021Quote: ... Catalytically dead mutant variant (D527A; D596A) was obtained by site-directed mutagenesis using QuikChange Lightning Multimutagenesis kit (Agilent), expressed and purified in the same way.
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bioRxiv - Molecular Biology 2021Quote: ... The constructed libraries were verified using the Agilent 2100 Bioanalyzer with DNA 1000 kit (Agilent, cat# 5067-1504) and quantified using KAPA Library Quantification Kits (KAPA Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... and the cytoplasmic RNA purified from RIP was tested for quality using a RNA 6000 nano kit (Agilent) on a Bioanalyzer 2100 (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA integrity was confirmed using an Agilent 2100 Bioanalyzer and the associated RNA 6000 pico kit (Agilent Technologies). Samples containing 4.5 μg of total RNA were depleted for ribosomal RNA using a Ribozero rRNA depletion kit (MRZGP126 ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng of bisulfite-treated mRNA (see above) was quantified using Agilent RNA 6000 Pico Kit (Agilent, #NC1711873) and reverse transcribed into cDNA using HiScript II Q Select RT SuperMix (Vazyme ...
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bioRxiv - Molecular Biology 2021Quote: ... Library quality and size distribution was assessed using the 2100 Bioanalyzer High Sensitivity DNA Kit (Agilent, 5067-4626) followed by qPCR quantification with the Kapa Library Quant Kit (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Quality control was performed on an Agilent 2100 bioanalyser using an RNA 6000 Pico LabChip kit (Agilent Technologies). The RNA integrity number (RIN ...
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bioRxiv - Microbiology 2020Quote: ... Bands were excised and purified for cloning with the StrataClone PCR Cloning kit (#240205, Agilent, Santa Clara, CA), following manufacturer’s guidelines ...
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bioRxiv - Genetics 2019Quote: ... and analyzed on an Agilent 2100 Bioanalyzer using Agilent 6000 RNA Nano Kit (Agilent Technologies, Santa Clara, California). cDNA was generated using the Superscript Double-Stranded cDNA Synthesis kit (ThermoFisher) ...
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bioRxiv - Immunology 2019Quote: Mutations were introduced into plasmid constructs using the QuickChange Site-Directed Mutagenesis Kit (Agilent Technologies, San Jose, CA) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2019Quote: All experiments were performed with a one-step enzymatic kit in a Stratagene Mx3005P qPCR system (Agilent Technologies). For this experiment Brilliant III ultra-fast qRT-PCR master mix (Agilent ...
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bioRxiv - Physiology 2019Quote: ... For QC and to quantify the library concentration we used both the BioAnalyzer (Agilent BioAnalyzer High Sensitivity Kit) and qPCR (Kapa Quantification kit for Illumina Libraries) ...
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bioRxiv - Genomics 2019Quote: ... and their quality and concentration were assessed using a 2100 Bioanalyzer with the High Sensitivity DNA Kit (Agilent) and a Qubit Fluorometer with the Qubit dsDNA HS Assay Kit (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... Library size distribution was evaluated using the High sensitivity dsDNA kit on a 2100 Bioanalyzer (Agilent, 5067-4626) and qPCR based library quantification was performed using the KAPA Library Quantification kit for Illumina platforms (KAPA Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... RNA concentration and quality was analysed with Agilent RNA 6000 Nano Kit at a Bioanalyzer 2100 (Agilent Technologies).
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bioRxiv - Biophysics 2019Quote: Point mutations were introduced into hamster SUR1 cDNA in pECE using the QuikChange site-directed mutagenesis kit (Stratagene). Mutations were confirmed by DNA sequencing ...
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bioRxiv - Microbiology 2019Quote: ... and Cy3-labeled cRNAs were synthesized using the Low Input Quick Amp Labeling kit (one-color, Agilent Technologies). Labeled probes were purified with RNeasy kit (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: ... The quality of the total RNA was analysed using an Agilent Bioanalyzer RNA 6000 Pico kit (Agilent Technnology). Poly(A ...
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bioRxiv - Cancer Biology 2019Quote: ... Variant calls were made with GATK UnifiedGenotyper using the mm9 reference sequence in regions targeted by the Agilent capture kit (version G7550_20110119_mm9 from Agilent). Calls with at least 14X read coverage and dual strand support were retained and further filtered using GATK VariantFiltration (arguments - clusterWindowSize 10 - cluster 3) ...
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bioRxiv - Genetics 2019Quote: The Cas9 high fidelity variants were generated by “QuikChange Multi Site-Directed Mutagenesis Kit” (Agilent Technologies, CA, USA) with primers listed in supplemental table 1 ...
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bioRxiv - Biophysics 2019Quote: ... HIV-1 MAG2A-EGFP was made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). HIV-1 MAG2A-ΔHBR-EGFP was generated from HIV-1 MAG2A-EGFP by deleting amino acids 18-32 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2019Quote: ... and were subjected to quality check with a Bioanalyzer 2100 using an RNA 6000 nano kit (Agilent Technologies). RNA sequencing was performed using Illumina HiSeq 4000 ® (for lung cancer tissue RNAs ...
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bioRxiv - Neuroscience 2019Quote: ... RNA was eluted by adding 100 µl Lysis Buffer and purified using the Absolutely RNA Nanoprep Kit (Agilent).
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bioRxiv - Genetics 2021Quote: ... and quality checked on Agilent Bioanalyzer using the Agilent RNA 6000 Nano kit (Agilent, Santa Clara, CA, USA). Single end ...
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bioRxiv - Genetics 2021Quote: Mutagenesis of the sequence encoding Sir3464-728 using the GeneMorph II EZClone Domain Mutagenesis Kit (Agilent, 200552-5) was performed by PCR on 9,5 µg of pACT2-SIR3464-728 with 20 cycles of amplification to allow low mutation rate ...
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bioRxiv - Genetics 2020Quote: ... The total RNA was amplified using the Sure Select Stranded RNA-Seq kit (Agilent Technologies; Santa Clara, CA) using standard protocols ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library size distributions were assessed using the Agilent Technology 2100 Bioanalyzer and the High Sensitivity DNA kit (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the quality and quantity of that pool was assessed via electrophoresis (High-Sensitivity DNA Kit and Agilent Bioanalyzer ...
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bioRxiv - Developmental Biology 2021Quote: ... product size distribution and quantity were assessed on a Bioanalyzer using a High Sensitivity DNA Kit (Agilent Technologies). A total of 140 pg of the amplified cDNA was fragmented using Nextera XT (Illumina ...
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bioRxiv - Immunology 2021Quote: ... RNA quality was checked using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies). Gene expression was analyzed using the NanoString’s nCounter digital barcode technology ...
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bioRxiv - Genomics 2021Quote: ... RNA integrity and purity were assessed using an Agilent 2100 Bioanalyser and RNA 6000 Pico Kit (Agilent, USA) and a Tapestation (Agilent) ...
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bioRxiv - Neuroscience 2020Quote: ... The V990M and P1090Q mutants were generated using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). For the GPCR regulation of these mutants ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting dscDNA was validated by determining size (∼1kb) using a BioAnalyzer DNA High Sensitivity kit (Agilent Technologies). Then ...