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Citations for Agilent :
301 - 350 of 3226 citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Mouse monoclonal antibody against Ki-67 (dilution 1:50; Code No. M7249, Dako, Glostrup, Denmark) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse and/or rabbit IgG (1:1000, 1μg/mL, diluted in antibody diluent, Agilent Technologies) served as negative control ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-p53 (Clone DO-7) (1:1000, #M7001, DAKO, Agilent, Santa Clara, California, USA) and mouse anti-beta Actin (Clone C4 ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-p53 (Clone DO-7) (1:1000, #M7001, DAKO, Agilent, Santa Clara, California, USA) and mouse anti-beta Actin (Clone C4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were stained with mouse monoclonal anti-CKAE1/AE3 antibody (Dako, M3515, 1:200 dilution), guinea pig polyclonal anti-doublecortin antibody (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... PAECs were immunolabelled using primary mouse monoclonal antibodies against CD31 (1:25; clone JC70A; Dako) and vWF (1:50 ...
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bioRxiv - Molecular Biology 2024Quote: ... a 1:4000 dilution of anti-mouse HRP-conjugated secondary antibody into blocking milk (DAKO) was applied to the membrane for 1h at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated for 1 hour at room temperature with biotinylated goat anti-mouse or goat anti-rabbit IgG antibodies (Dako, 1:500), washed 3 times and incubated with streptavidin Cy3 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated antibodies were used as secondary antibodies (Swine anti-rabbit 1:3000, Goat anti-mouse 1:5000, Dako, Glostrup, Denmark). Membranes were developed with Clarity Western ECL Substrate 1:1 (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 min) before incubation with either rabbit anti-mouse IgG (1.3 µg ml−1, 1% BSA in PBST; Agilent, P026002-2), goat anti-rabbit IgG (250 ng ml−1 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in primary antibody (anti-Alk1 Rabbit Abcam #ab68703, 1:100; anti-CD31 Mouse Dako #M0823, 1:500) over-night at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... The concentration and size distribution of Hi-C library DNA after PCR amplification was determined by tapestation (Agilent Technologies), and the Hi-C libraries were sequenced on Illumina Hi-Seq 2500 or Illumina Hi-seq 4000 at 50 cycles.
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Physiology 2020Quote: ... 20 μL samples were injected into Hi-Plex H column (300×7.7 mm; 8 μm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was DNase treated using Turbo DNA free kit according to manufacturer’s protocol (Thermo Fischer scientific) for making cDNA using Accuscript hi-fidelity cDNA synthesis kit (Agilent). The qRT-PCR was set up using brilliant III ultra-fast SYBRgreen qPCR master mix in Mx3005P qPCR system Agilent ...
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bioRxiv - Genetics 2020Quote: ... Synthesis of cDNA was carried out with the AccuScript Hi-Fi cDNA Synthesis Kit (Agilent Technologies, Santa Clara, USA) using Oligo(dT ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of pcDNA3.1-NS3-mCherry-HIS plasmid was done by using PfuUltra HotStart DNA Polymerase (Agilent, Cat #: 600390) and forward and reverse primers (table 2 ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Neuroscience 2020Quote: Mouse brains were analyzed as free-floating sections to localize immunoreactivity for GFAP (Z0334, Dako/Agilent; 1:10,000 primary, 1:10,000 secondary), Iba-1 (019-19741 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight at 4° C with primary antibodies (mouse-anti-Ataxin-3, 1:2500, clone 1H9, MAB5360, MerckMillipore, Darmstadt, Germany; rabbit-anti-Ubiquitin, 1:500, Z0458, Dako, Jena, Germany). After washing the membranes were incubated with secondary antibodies (peroxidase affinePure donkey anti-mouse IgG H+L ...
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bioRxiv - Cell Biology 2022Quote: ... IgG1a monoclonal antibody in PBS with 1% BSA followed by a bridge step labelling with a polyclonal Rabbit Anti-Mouse Immunoglobulins (Dako, 1:50) and protein A-gold 10 nm (UMC Utrecht ...
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bioRxiv - Immunology 2020Quote: ... 1:4000, rat anti-mouse IgG3-biotin (Becton, Dickinson, Franklin Lakes, New Jersey) 1:2000 followed by streptavidin-HRP (Dako, Glostrup, Denmark) 1:1000 incubated at 37°C for 1h.
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP, Dako, 1:2500), and washed 3 times 15 minutes in TBST again before chemiluminescence detection ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP, Dako, 1:2500), and washed 3 times 15 minutes in TBST again before chemiluminescence detection ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, Dako, Japan) and HRP goat anti-rat IgG antibody (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... GFAP immunohistochemistry for astrocytes (rabbit anti–mouse GFAP polyclonal antibody 1:13000 for 24 min; DAKO) and IBA-1 for microglia (rabbit polyclonal antibody 1:1000 for 24 min ...
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bioRxiv - Cancer Biology 2022Quote: We performed immunochemistry assays using mouse anti-human ki67 antibody (M7240, DAKO, 1/200 at pH9) in a series of paraffin-embedded tissue blocks of HGSOC ...
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bioRxiv - Biochemistry 2022Quote: ... and aSN (primary antibody: anti-Syn-1, BD Transduction Laboratory, secondary antibody: anti-mouse-HRP, Dako). The interaction between endogenous aSN and the endogenous PMCA was studied in the extracts from C57BL/6 mice as described above.
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used for staining of hBVOs were mouse anti-hCD31 (dilution 1:100, Dako, M0823), rabbit anti-hPDGFR-β (dilution 1:100 ...
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bioRxiv - Microbiology 2021Quote: ... After a 30 min incubation with the secondary rabbit-anti mouse IgG antibody (1:25) (Dako) followed a 30 min incubation step with alkaline-phosphatase-anti-alkaline-phosphatase (1:40 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 hour incubation at room temperature with secondary antibodies (HRP-conjugated anti-mouse secondary (Dako #P0447); HRP-conjugated anti-rabbit secondary (Dako #P0448) ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibody incubation was performed in polyclonal rabbit anti mouse – HRP antibody 1:2000 (Dako, P0260) or polyclonal goat anti rabbit – HRP antibody 1:2000 (Dako ...
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bioRxiv - Cell Biology 2023Quote: ... Both membrane parts were incubated with a rabbit-anti-mouse-(1:1000; P0261; DAKO, Agilent, USA) and swine-anti-rabbit-HRP conjugated secondary antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Both membrane parts were incubated with a rabbit-anti-mouse-(1:1000; P0261; DAKO, Agilent, USA) and swine-anti-rabbit-HRP conjugated secondary antibody (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used were: Goat anti-mouse IgG HRP (Cat no. P0447, Dako, 1:1500 dilution) and Goat anti-rabbit IgG HRP (Cat no ...
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bioRxiv - Cell Biology 2023Quote: ... we performed immunochemistry assays using mouse anti- human ki67 antibody (M7240, DAKO, 1/200 at pH9) in a series of paraffin-embedded tissue blocks of HGSOC ...
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bioRxiv - Microbiology 2020Quote: ... Rabbit/Mouse (Agilent). This staining procedure can be used for sequential double staining of two different antigens with primary antibodies raised in the same species by incorporating a blocking step between the first and second antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit/mouse (Dako) was used to visualize the antibody staining ...
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bioRxiv - Immunology 2024Quote: ... Rabbit/Mouse (Agilent) and counterstained with hematoxylin (Wako) ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Systems Biology 2021Quote: ... respectively) on a 300 × 7.7 mm PL Hi-Plex H+ (8 µm particle size) column (Agilent Technologies, Santa Clara, CA) and 4 x 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A 20 µl sample was injected into the Hi-Plex H column (300×7.7 mm; 8 µm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: The CAN97-83 HMPV P construct was cloned into the plasmid pET 302/NT-His between the cleavage sites EcoRI and XhoI and expressed in BL21(DE3) CodonPlus RIL cells (Agilent) overnight at 37 °C in terrific broth (TB ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Cell Biology 2021Quote: ... The three missense mutations identified were introduced into a previously described pcDNA3.1-CD33-V5/HIS vector using a QuikChange XL kit (Agilent 200517) with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis was performed on pGAPZα-His-MspGH25 vector according to the instructions of the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Genetics 2022Quote: ... the other mtr4 mutant plasmids were constructed by site-directed mutagenesis of the MTR4 HIS CEN6 plasmid (pAC4096) with the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the corresponding missense mutations ...
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bioRxiv - Biochemistry 2023Quote: ... R448A and N455A were introduced in the pETNKI-his-JBP1 JDBD plasmid using a modified QuickChange site-directed mutagenesis method (Agilent). Forward and reverse mutagenesis primers containing the single point mutations were purchased from ThermoFisher Scientific ...