Labshake search
Citations for Agilent :
301 - 350 of 599 citations for 9 Z 12 E OCTADECADIENOIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Prior to immunohistochemistry antigen retrieval was performed using Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent). Quenching of endogenous peroxidase was performed by a 10 min incubation with Peroxidase-Blocking Solution (Dako REAL S2023) ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids expressing gfpA206K fusions to mgrB mutants (pSY3-9, pSY72-75, pJC1-7, pTG1-15) were created by either QuikChange site-directed mutagenesis (Stratagene) or Inverse PCR [58] using pAL38 as template ...
-
bioRxiv - Immunology 2021Quote: ... Epitope retrieval was performed at 97C for 10 min with the Dako Target Retrieval Solution pH 9 (Agilent, S236784-2) on a Lab Vision PT Module (Thermo Fisher Scientific).
-
bioRxiv - Genomics 2022Quote: ... Hi-C material was then amplified from the beads with 7-9 cycles of PCR using Herculase II Fusion DNA polymerase (Agilent) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: AAV was produced by HHMI-Janelia Viral Tools using a PEI triple transfection protocol into AAV293T cells (an ITR-containing plasmid, 2/9 capsid helper from UPenn Vector Core, and the E1-deleted pHelper plasmid from Agilent). The cells were grown under serum-free conditions (three 150mm culture dishes at ∼3×107 cells/dish for each 100 µl batch) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Heat-mediated epitope retrieval was performed by incubating slides in a beaker containing Tris EDTA solution (pH 9; Dako, #S2367) in a pressure cooker for 20 minutes ...
-
bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 (Dako Target Retrieval Solution, S236784-2) in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 (Dako Target Retrieval Solution, S236784-2) in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2024Quote: ... dissolved in 15 µl pyridine and derivatized with 30 µl N-methyl-N-(trimethylsilyl)trifluoracetamid (MSTFA) before being analyzed by GC-MS (Agilent 7890B GC-Agilent 5977N-MSD) as previously described (Berghoff et al. ...
-
bioRxiv - Neuroscience 2021Quote: ... (12) mouse anti-PCNA was used at 1:1000 (M0879; Dako Immunochemicals). This antibody was raised to a synthetic amino acid sequence (LVFEAPNQEK ...
-
Sex Differences in Brain Tumor Glutamine Metabolism Reveal Sex-Specific Vulnerabilities to TreatmentbioRxiv - Cancer Biology 2021Quote: Derivatized samples were run on an Agilent 7890A GC coupled to an Agilent 5975C MS and data was acquired and analyzed in MSD ChemStation E.02.02.1431 (Agilent). The GC temperature program was set to ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in DMEM with 10% of FCS and transferred into xCELLigence microplates (E-plate 16, Agilent) at the density of 20 000 cells for real-time analysis of cell adhesion and growth ...
-
Sex Differences in Brain Tumor Glutamine Metabolism Reveal Sex-Specific Vulnerabilities to TreatmentbioRxiv - Cancer Biology 2021Quote: Derivatized samples were run on an Agilent 7890A GC coupled to an Agilent 5975C MS and data was acquired and analyzed in MSD ChemStation E.02.02.1431 (Agilent). The GC temperature program was set to ...
-
bioRxiv - Neuroscience 2022Quote: ... Derivatized samples were run on an Agilent 7890A GC coupled to an Agilent 5975C MS and data was acquired and analyzed in MSD ChemStation E.02.02.1431 (Agilent). The GC temperature program was set to ...
-
bioRxiv - Systems Biology 2023Quote: ... The cells were plated at 15,000 cells/well onto fibronectin-coated (5 μg/cm2) xCELLigence E-plate (Agilent) wells in serum free medium ...
-
bioRxiv - Cancer Biology 2023Quote: ... 30,000 LM7 or LM7-KO cells were added to each well of a 96 well E-Plate (Agilent) in complete RPMI ...
-
bioRxiv - Plant Biology 2023Quote: ... Raw GC/MS files were exported to NetCDF format using Agilent MSD ChemStation software (Revision E.02.01.1177; Agilent Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... All tissue slices were routinely stained with hematoxylin and eosin (H&E) using the Dako Coverstainer (Agilent Technologies) and were reviewed by an experienced pathologist ...
-
bioRxiv - Microbiology 2023Quote: Cells were seeded at 3,000 cells/well in 150 μL of complete media (above) in E-plates (Agilent) and grown overnight while being monitored with an xCELLigence SP system (Agilent) ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by staining with hematoxylin and eosin (H&E) (Eosin, Dako CS701, Hematoxylin Dako S3309, bluing buffer CS702). The brightfield images were taken on a Leica DMI8 whole-slide scanner at 10x resolution.
-
bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
-
bioRxiv - Bioengineering 2023Quote: ... ITO slides were then stained for H&E and imaged using the Cytation 5 (Agilent, Santa Clara, CA) and Epsilon scanner at 3000 dpi to select regions of interest using the open-source program ...
-
bioRxiv - Bioengineering 2020Quote: ... The concentration of the ester in the n-hexadecane phase was quantified using a gas chromatography-mass spectrometry (GC-MS, Agilent Technologies 6890N, Santa Clara, CA) equipped with an HP-5 column (60m×0.25 mm ...
-
bioRxiv - Biochemistry 2023Quote: ... Metabolomics data were acquired using an Agilent Infinity II/6495 LC-MS/MS fitted with an InfinityLab Poroshell 120 HILIC-Z column (Agilent, 2.1 x 150 mm, 2.7 Micron). The mass spectrometer was operated in both positive and negative ion modes using polarity switching ...
-
bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
-
bioRxiv - Plant Biology 2020Quote: ... RT-PCR was performed with 1μL of diluted cDNA (1:9) in a Brillant III ultra fast SYBRgreen QPCR master mix (Agilent Technologies, USA) using a StepOnePlus Real-Time PCR System (Applied biosystems ...
-
bioRxiv - Neuroscience 2021Quote: ... 3 μm paraffin-embedded tissue sections were either dewaxed and subjected to antigen retrieval treatment with Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent) or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005 ...
-
bioRxiv - Cell Biology 2020Quote: ... Prior to immunohistochemistry antigen retrieval was performed using Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent). Quenching of endogenous peroxidase was performed by a 10 min incubation with Peroxidase-Blocking Solution (Dako REAL S2023) ...
-
bioRxiv - Immunology 2019Quote: ... Antigen retrieval was performed either with Proteinase K (for F4/80) or boiling the sections in a high pH antigen retrieval buffer (Dako Target retrieval Solution, pH 9, Dako, Denmark) (for Podocalyxin ...
-
bioRxiv - Neuroscience 2022Quote: ... of RF energy with varying input power to the antenna (9-68 W, measured by a power meter (U2001A Power Sensor connected to N9912A Field Fox Spectrum analyzer, Agilent/Keysight) and a bidirectional coupler (778D ...
-
bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Heat-induced antigen retrieval (HIER) was performed in a coverslip jar containing 1x Dako pH 9 Antigen Retrieval Buffer (Agilent, S2375) while using a PT module filled with 1x PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... 6.5-9) and concentration (5-50 ng/µl; 260/280 values >1.7) were evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, CA) and Nanodrop (Thermo-fisher ...
-
bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 with Dako Target Retrieval Solution (Agilent, USA), in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2020Quote: ... for amino acids and an Eclipse Plus C18 (1.8 μm; Agilent) for TCA and PPP intermediates ...
-
bioRxiv - Microbiology 2021Quote: ... The fatty acid profiles were analyzed by gas chromatography (Agilent 7890A) using the RTSBA6 method/library ...
-
bioRxiv - Genetics 2021Quote: ... and organic acids by a dual-wavelength absorbance detector (Agilent G1314F).
-
bioRxiv - Physiology 2021Quote: Amino acid concentrations were determined with high-performance liquid chromatography (Agilent Technologies 1100 HPLC System ...
-
bioRxiv - Neuroscience 2022Quote: ... Anti glial fibrillary acid protein (Abcam, GFAP, Dako Z0334 1:1000); Anti CD68 (Biorad MCA1957 ...
-
bioRxiv - Neuroscience 2022Quote: ... and glial fibrillary acid protein (GFAP; Z0334, Dako, RRID:AB_10013382, 1:500) were performed as previously described (Muñoz-Manchado et al ...
-
bioRxiv - Molecular Biology 2023Quote: ... Silica beads functionalized with phenyl-boronic acid (Bondesil-PBA 40µm, Agilent) were used for glycopeptides enrichment at an optimized ratio of 1:2.5 sample:PBA beads w/w ...
-
bioRxiv - Neuroscience 2021Quote: ... The quality of the cDNA was assessed on a Tape 12 Station (Agilent). A total of 1 ng purified cDNA was used as starting material for the library preparation ...
-
bioRxiv - Immunology 2021Quote: ... 96 deep well polypropylene plates (0.7 ml) with flat bottom and square wells (EK-2074, E&K Scientific; 201242-100, Agilent), or 24 well cell culture clear plates with flat bottom and circular wells (T1024 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-E-cadherin (diluted 1:100) antibody (Ab) and anti-vimentin Ab (1:1000) were purchased from DakoCytomation, mouse anti-p65 Ab (1:500 ...
-
bioRxiv - Microbiology 2023Quote: Vero TMPRSS2 cells were plated in 96-well tissue culture treated E-Plate VIEW plates (#300-601-020, Agilent) to be subconfluent at time of assay ...
-
bioRxiv - Cancer Biology 2023Quote: All cytotoxicity assays were performed using the xCELLigence RTCA MP analyzer and 96 well PET E-plates (Agilent Technologies). Assays were carried out in RPMI containing 10% fetal calf serum ...
-
bioRxiv - Bioengineering 2021Quote: ... mRNA was isolated from high quality total RNA samples with RIN > 9 and 28S:18S > 1 (measured with an Agilent 2100 Bioanalyzer) using poly-T oligonucleotide beads ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... a 2uL aliquot from each plated sample was diluted 1:9 on a Bravo liquid handling platform (Agilent, Santa Clara, CA, USA). A 5 μL aliquot from each diluted sample was arrayed into a 384 well plate for input into the mNGS library prep ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells used for all experiments were ≤9 passages from stock and tested negative for mycoplasma contamination (Agilent MycoSensor QPCR Assay Kit, 302107).