Labshake search
Citations for Agilent :
301 - 350 of 3532 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... mCTLA-4 cDNA was sub-cloned into the pCMV-Tag4A vector (Agilent Technologies) and an exon3 deletion construct was made by the KOD Plus Mutagenesis Kit (TOYOBO) ...
-
bioRxiv - Cancer Biology 2023Quote: ... with small RNA ladder (4-150nt) were used (cat no. 5067-1550, Agilent), all accessory reagents were also from Agilent (cat no ...
-
bioRxiv - Immunology 2023Quote: ... The tissue was then incubated overnight at 4°C with anti-CD4 (Dako) in a 1:10 dilution ...
-
bioRxiv - Cancer Biology 2022Quote: ... Slides were next incubated overnight at 4°C with anti-PCNA antibody (DakoCytomation, clone PC10 ...
-
Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... Lactate content was monitored using a Synergy 4 automatic microplate reader (Agilent BioTek) at 570 nm.
-
Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... Nonspecific binding was minimized by incubating the sections with 4% Goat Serum (Agilent) for 30 min ...
-
bioRxiv - Immunology 2024Quote: ... Each sample was run on a 4% agarose gel and TapeStation 4150 (Agilent) to verify the presence of PCR products of the appropriate size (383 bp) ...
-
bioRxiv - Physiology 2019Quote: ... for 15 minutes and then incubated overnight at 4°C with primary antibodies diluted in DAKO antibody diluent: anti-Insulin (DAKO, 1:1000), anti-BRD4 (Bethyl laboratories ...
-
bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
-
bioRxiv - Molecular Biology 2022Quote: ... Adapter dilution (1:4) and PCR amplification (26 cycles) were optimized in a pilot experiment using Bioanalyzer DNA1000 (Agilent, Sant Clara, USA) chips as a read out of library size and quantity ...
-
Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
-
bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in quadruplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
-
bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in triplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
-
bioRxiv - Plant Biology 2019Quote: ... PAD4LLD was obtained by site-directed mutagenesis on pENTR/D-TOPO PAD4 according to the QuikChangeII site-directed mutagenesis manual (Agilent) (See Table S1 for primers) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Immunohistochemistry (IHC) for SEMA4C was performed using a rabbit anti-SEMA4C primary antibody (#AF6125, R & D Systems) on an autostainer (Dako). Detection was achieved using the Envision rabbit detection system (Dako ...
-
bioRxiv - Immunology 2019Quote: ... 5×105 CD8+ T-cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose ...
-
bioRxiv - Plant Biology 2020Quote: ... Eluting metabolites were fragmented in electron impact mode with an ionization voltage of 70 eV and data was acquired using MSD ChemStation software (revision D.01.02.SP1, Agilent Technologies). Analytes were identified based on their mass fragmentation patterns by comparison with those of authentic standards using the National Institute of Standards and Technology Mass Spectral Search Program (version D.05.00) ...
-
bioRxiv - Neuroscience 2022Quote: ... Libraries were analyzed with Qubit and fragment size was accessed using the high sensitivity D-5000 screen tapes (5067-5592 Agilent) on a Tapestation.
-
bioRxiv - Developmental Biology 2023Quote: ... 60sal- MUT2 and 60sal-MUT3 were generated from pENTR/D-TOPO-UAS(5x)-60sal(1x) using Pfu polymerase and Quick Change Site-Directed Mutagenesis (Stratagene). Sequences of 6 nucleotides were changed C to A and G to T using the oligonucleotide pairs 60sal- MUT1 Fw and 60sal-MUT1 Rv ...
-
bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
-
bioRxiv - Genomics 2023Quote: ... and mock-edited control cells (CTRL) were plated on poly-D-lysine coated 96-well Seahorse assay plates (XF96, Agilent) in DMEM (Gibco A144300 ...
-
bioRxiv - Neuroscience 2023Quote: ... and 5.47*105 cells/cm2 were plated in each well of a poly-D-lysine-coated Seahorse XFe24 V7 PS Cell Culture Microplate (Agilent) or a 24-well tissue culture plate ...
-
bioRxiv - Cancer Biology 2022Quote: ... clone DO-7 (M7001, DAKO) and the BOND™ Polymer Refine Detection System (DS9800 ...
-
bioRxiv - Cancer Biology 2021Quote: ... organoids were seeded in 3 μL matrigel per well in XF24 cell culture microplates (Seahorse Bioscience). 1 hour before the measurements ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were washed 3 times with TNT and mounted with a fluorescence mounting medium (Dako, S3023) under coverslips.
-
bioRxiv - Bioengineering 2020Quote: ... The sections were deparaffinized in xylene and treated for 3 hours with antigen retrieval solution (Dako) at 80°C ...
-
bioRxiv - Immunology 2019Quote: ... Diluted monoclonal mouse anti-Ki-67 antibody (TEC-3 antibody [Dako Code M7249]; Dako, Glostrup, Denmark) was then applied and slides incubated overnight at room temperature in a humidified chamber ...
-
bioRxiv - Immunology 2019Quote: ... Diluted monoclonal mouse anti-Ki-67 antibody (TEC-3 antibody [Dako Code M7249]; Dako, Glostrup, Denmark) was then applied and slides incubated overnight at room temperature in a humidified chamber ...
-
bioRxiv - Molecular Biology 2019Quote: ... 95-3% 6.5 minutes) using an Agilent 1200 G1312 Binary pump (Agilent Technologies, San Jose, CA). The column was coupled to a home-built electrospray ionization source and LTQ-Velos Orbitrap mass spectrometer (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... and (3) the RNA integrity was measured using a Bioanalyser 2100 (Agilent, Santa Clara, CA, USA). The primers used in this assay are listed in Supplementary Table S2 ...
-
bioRxiv - Neuroscience 2021Quote: ... Lipids were resolved on a 3 150 mm XDB-C8 column (5 μM particle size) (Agilent) at flow rate 0.4 mL/min ...
-
bioRxiv - Biochemistry 2021Quote: ... and 50 μl of sample were injected onto a Bio SEC-3 300 column (Agilent Technologies) pre-equilibrated with the running buffer (20 mM HEPES ...
-
bioRxiv - Immunology 2020Quote: ... followed by incubation in 3% hydrogen peroxide and in serum-free protein block solution (Dako, X0909) before adding primary antibody for 1 hour at room temperature ...
-
bioRxiv - Plant Biology 2020Quote: ... using a Zorbax Eclipse XDB-C18 column (50 x 3 x 4.6 mm, 1.8 μm; Agilent). Formic acid (0.05% ...
-
bioRxiv - Molecular Biology 2020Quote: ... with a specific primer (5’-CCTACACGACGCTCTTCC-3’) using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). Then ...
-
bioRxiv - Neuroscience 2021Quote: ... After 3 final washing steps sections were mounted with Dako fluorescent mounting medium (Agilent Technologies, USA) and sealed with nail polish ...
-
bioRxiv - Biochemistry 2019Quote: ... OCR and ECAR were measured 3 times every 9 minutes using a XFe96 Analyzer (Seahorse Bioscience) at a baseline and after addition of each drug ...
-
bioRxiv - Immunology 2021Quote: ... followed by exposure to 3-amino-9-ethylcarbazole substrate (AEC, Dako, Agilent, Santa Clara, CA, USA). Sections were counter-stained with Mayer’s hematoxylin ...
-
bioRxiv - Immunology 2021Quote: ... followed by exposure to 3-amino-9-ethylcarbazole substrate (AEC, Dako, Agilent, Santa Clara, CA, USA). Sections were counter-stained with Mayer’s hematoxylin ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were analyzed 3 days after transfection using flow cytometry (Dako CyAN ADP, or ACEA Quanteon), as described30,64 ...
-
bioRxiv - Neuroscience 2022Quote: ... the sections were mounted on slides with Dako fluorescent mounting medium (Agilent-a o S3 3). The following primary antibodies were used ...
-
bioRxiv - Neuroscience 2022Quote: ... washed in PBS (3 × 5min) and incubated with serum free protein block (Dako, Cat# X090930-2) for 1h at RT ...
-
bioRxiv - Immunology 2023Quote: ... washed 3 times in PBS (10 minutes each) and mounted using Dako mounting medium (S3023; Agilent). Primary antibodies and detection reagents used ...
-
bioRxiv - Immunology 2023Quote: ... Immunolabeling was visualized by 3-amino-9-ethylcarbazole substrate (AEC, Dako, Agilent, Santa Clara, CA, USA), producing a red-brown signal and sections were counter-stained with Mayer’s hematoxylin ...
-
bioRxiv - Immunology 2023Quote: ... Immunolabeling was visualized by 3-amino-9-ethylcarbazole substrate (AEC, Dako, Agilent, Santa Clara, CA, USA), producing a red-brown signal and sections were counter-stained with Mayer’s hematoxylin ...
-
bioRxiv - Cancer Biology 2024Quote: ... The 3100 OFFGEL Fractionator and the OFFGEL Kit pH 3-10 (Agilent Technologies, Santa Clara, CA) was used following a 24-well set up ...
-
bioRxiv - Cancer Biology 2019Quote: ... For 8 other samples (1 normal and 3 tumor regions each from 2 patients) only DNA was isolated and targeted panel sequencing (Agilent SureSelect run on a HiSeq) was performed for 257 genes ...
-
bioRxiv - Pathology 2023Quote: ... Sections from the first level (main experiment) were stained for smooth muscle alpha-2 actin (ACTA2) and galectin 3 (LGALS3) using mouse monoclonal anti-ACTA2 (Dako, cat. no. M0851, 1:100) after blocking with Fab fragment (Jackson ImmunoResearch ...
-
bioRxiv - Neuroscience 2023Quote: ... Afterwards the sections were incubated in a humidified chamber (overnight 4°C) in a peroxidase conjugated immunoglobulin against UEA (DAKO, P289; 1:50). Finally ...
-
Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...