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Citations for Agilent :
3401 - 3450 of 3768 citations for 8 CHLORO 2 CHLOROMETHYL 6 TRIFLUOROMETHYL IMIDAZO 1 2 A PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... For all experiments the TIMS elution voltages were calibrated by known 1/K0 values from at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Biochemistry 2024Quote: ... The signals were obtained with 8 cm-1 spectral resolution (unless specified otherwise) by performing 35 consecutive readings per measurement in 4,000-650 cm-1 range using MicroLab PC software (Agilent Technologies) and further processed with Spectragryph (F ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixture was acidified by addition of 1% trifluoroacetic acid (TFA) and desalted on a reversed phase (RP) C18 OMIX tip (Agilent). The tip was first washed 3 times with 200 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 hour before being placed into a Seahorse XFe24 Analyzer (Agilent). For oxygen consumption rate (OCR ...
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bioRxiv - Neuroscience 2023Quote: ... The steroid extraction was performed as earlier described for plasma [39] with a further modification of the CSF analysis to encompass a different size of solid-phase extraction columns (100 mg Bond elute C18 solid-phase extraction cartridges; 1 ml; Agilent) and therefore corresponding volume changes for conditioning (1 ml MeOH followed by 2×1 ml dH2O) ...
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bioRxiv - Microbiology 2023Quote: ... skimmed milk powder and probed with desired primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1:5000 (Dako Agilent). Protein bands were detected using a G:BOX (Syngene ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal rabbit anti-mouse secondary antibody IgG/HRP (P0260; Agilent Technologies/Dako) diluted 1:2000 in 3% BSA.
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bioRxiv - Genetics 2023Quote: ... the Prab-3p::hpk-1 plasmid was used and mutations were performed with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All the assembled plasmids were validated by sequencing prior to microinjection ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated for 30 minutes with the rabbit anti-human HES1 antibody diluted 1:100 in a low background diluent (Dako). A linker rabbit was used to amplify the signal ...
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bioRxiv - Cancer Biology 2023Quote: ... ~100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with either horse radish peroxidase (HRP)-labeled anti-rabbit IgG (1:20000, Agilent Dako, Poland) or HRP-labeled anti-mouse IgG (1:20000 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with either horse radish peroxidase (HRP)-labeled anti-rabbit IgG (1:20000, Agilent Dako, Poland) or HRP-labeled anti-mouse IgG (1:20000 ...
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bioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent).
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were incubated overnight with the following primary antibodies: rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:6,000, DAKO, UK), rabbit polyclonal anti-allograft inflammatory factor 1 (IBA1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were incubated in the following primary antibodies for 3 h at 24L: gastrin (1:1000, Cat #A0568, A0568, AB_2757270, Dako Agilent Technologies), Chromogranin A (1:100 ...
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bioRxiv - Immunology 2023Quote: ... and Seahorse XF Cell Mito Stress Test Kit (catalog number 103015-100, contains 1 each of oligomycin, FCCP, and rotenone/antimycin A.) were all purchased from Agilent Technologies ...
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bioRxiv - Physiology 2023Quote: ... Membranes were then washed in TBST and incubated with the secondary HRP-conjugated antibodies: goat anti-rabbit (1∶1000, P0448, DAKO) or polyclonal anti-mouse (1∶2000 ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with a goat anti-rabbit HRP-conjugated secondary antibody (Dako P0448, 1:2000 dilution in 5% BSA-PBST) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Shimadzu LC-10AT; autosampler: HiP sampler G1367A, T = 4°C, 10 µL injection; flow rate: 1 mL/min; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with 1x PBST for 5 min and incubated with secondary antibodies anti-mouse-HRP (1:10,000) (Agilent Dako, #P0447) and anti-rabbit-HRP (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality was determined by running 1 μL of the sample on a BioAnalyzer RNA Nano chip (Agilent, No. 5067-1511) for RIN ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... The percentage of the genome fragmented to sizes between 1 – 4 kb was then empirically measured by fragmenting 1 µg of genomic DNA followed by quantification on a Genomic DNA ScreenTape assay (Agilent). Hpy166II fragments 41% of the genome to within the target size range ...
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bioRxiv - Plant Biology 2023Quote: CDS sequences from PhDEF and PhGLO1 were amplified from Petunia x hybrida R27 inflorescence cDNAs with primers MLY2382/MLY2383 and MLY2384/2385 respectively (Supplementary Table 1) and cloned into the in vitro translation vector pSPUTK (Stratagene) by NcoI/XbaI restriction ...
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2023Quote: ... and kept at 4°C overnight with primary antibodies (chicken anti-GFP, Aves Labs, 1:4000; rabbit anti-GFAP, Agilent Pathology Solutions ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue samples were incubated for 60 min at room temperature with primary antibodies (Monoclonal Mouse Anti-Human Ki67, clone MIB-1, Dako North America Inc. ...
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bioRxiv - Systems Biology 2023Quote: ... we calibrated the TIMS elution voltages by known 1/K0 values from at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-incubated for 1 h and treated in Agilent Seahorse XF Base Medium (Agilent Technologies, cat#103334-100), which is optimized for extracellular flux assays and contains thiamine at 8.2 μM concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Double immunofluorescence for activated pro- and anti-inflammatory microglial cells was performed as above with rat anti-CD86 (M1; 1:1600 dilution, Dako) and goat anti-CD206 (M2 ...
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bioRxiv - Plant Biology 2023Quote: ... RNA integrity was confirmed by agarose (1% w/v) gel electrophoresis and by microfluidic electrophoresis using a Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... the first-strand complementary DNA (cDNA) was synthesized from 1 μg of RNA using oligo (dT) primers with Reverse Transcriptase (Agilent). Quantitative reverse transcription polymerase chain reaction (qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed in PBS and incubated in DAPI (1:500) for 5 min before mounting with the immunofluorescence mounting media (DAKO).
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bioRxiv - Genetics 2023Quote: ... Membranes were labelled with primary antibody for 1 hour at room temperature or overnight at 4°C followed by incubation with HRP-conjugated secondary antibodies (Dako). Membranes were developed using the Western Lightning ECL system (Perkin Elmer) ...
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bioRxiv - Microbiology 2023Quote: Log phase cultures of cells grown without inducer were diluted to an OD600 of 0.05 and grown in 96-well plates for 24 h at 30°C with constant shaking on a BioTek Cytation 1 plate reader (Agilent), measuring the absorbance at 600 nm every 30 min for three technical replicates for each strain ...
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bioRxiv - Cell Biology 2023Quote: ... Kidneys were equilibrated at 37°C for 1 h in a non-CO2 incubator and then assayed on a Seahorse XFe24 Analyzer (Agilent) following a 12-min equilibration period ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient-matched primary tumor tissues and PDTOs were immunostained for diagnostic NSCLC markers with antibodies against cytokeratin (CK)7 (mouse, M7018, 1:100; Dako), CK5/6 (mouse ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous peroxidases were blocked by incubation with 3 % H2O2 and then blocked for 1 h (Dako Serum-free protein block). Sections were then incubated with the 1st primary antibody (FABP7 ...
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bioRxiv - Immunology 2023Quote: ... 10 µg/mL of either mouse anti-human LAIR-1 (clone Dx26; purified in house) or mouse isotype control IgG1 (Dako) diluted in PBS+1% BSA buffer were incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... For staining of macrophages and platelets the aortic tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times (5 min) in TBST prior to addition of secondary antibody (goat anti-rabbit HRP 1:10,000; Dako - P00448). Blots were washed 3 times in TBST prior to development using SuperSignal West Pico Plus substrate (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... The library profiles were checked using High Sensitivity NGS Fragment Analysis kit (1-6000bp) (Agilent Technologies, reference DNF-474-1000) on the 5300 Fragment Analyzer System ...
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bioRxiv - Cell Biology 2024Quote: ... Qualitative and quantitative measurement of cDNA was conducted with the High Sensitivity NGS Fragment 1-6000bp kit on a 48-channel Fragment Analyzer (Agilent) and 1x dsDNA kit on the Qubit 4 Fluorometer (ThermoFisher) ...