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Citations for Agilent :
3301 - 3350 of 3556 citations for 6 Methoxy 3 4 Dihydro 2H Quinoxaline 1 Carboxylic Acid tert Butyl Ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... The TEV eluates were then incubated with a fresh 1:100 dilution preparation of RNaceIt (RNase A and T1 mixture; Agilent, 400720) for exactly 5 minutes at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were treated with 1:10 normal goat serum in PBS for 15 minutes and incubated for 60 minutes with 1:200 diluted Rabbit anti-human thyroglobulin (Dako, Denmark) or 1:400 diluted Rabbit anti-human calcitonin (Dako ...
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bioRxiv - Cancer Biology 2021Quote: WT and CCN4-KO melanoma cells were cultured overnight (1 × 104 cells/well) in Seahorse XFe96 cell culture microplates (Agilent, CA) with complete DMEM medium ...
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bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies were horseradish peroxidase-conjugated goat anti-mouse or goat anti-rabbit (used at dilution of 1:2,000; Dako UK, Ltd.). Reactive proteins were visualized by chemiluminescence with ECL plus (Amersham plc).
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bioRxiv - Immunology 2021Quote: ... sections were washed three times in 1 X PBS for 5 minutes and the sections allowed to dry slightly before mounting with DAKO mounting medium (Agilent Technologies) and allowing to airdry overnight in the dark at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... for 15 min and subsequently replaced with anti-249T-P pAbs diluted at 1:250 with antibody diluent solution (Agilent Technologies). Slides were incubated at 4°C overnight and then washed and visualized with DAB+ substrate chromogen (Agilent Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... The dried FAME samples were dissolved in 20 μL dichloromethane and analysed by injection of 1 μL into a GC-MS (GC-6890N, MS detector-5973; Agilent Technologies) using a ZB-5 column (30 m × 25 mm × 25 mm ...
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bioRxiv - Microbiology 2019Quote: ... the ciliate samples were added to a 1:1000 dilution of fluorescein isothiocyanate (FITC) conjugated rabbit/ anti-mouse Ig (DAKO, Denmark) and incubated for 1h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... cDNA was subjected to quality control using 1 µl of amplified cDNA on an Agilent 2100 BioAnalyser and Agilent High Sensitivity DNA kits (Agilent Technologies). 5 µl of cDNA per cell was cleaned using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... 1) were elucidated by 1D and 2D NMR [Varian MERCURY plus AS400 (400 MHz)] and HRTOF-MS spectroscopy (Agilent 1200/6530) (Supplemental Table 1).
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bioRxiv - Synthetic Biology 2021Quote: ... Desorption was performed at 250 °C for 1 minute (splitless mode) in the injection port of a 6890N gas chromatograph (Agilent Technologies). After desorption ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing was performed on genomic DNA from Patients 1 and 2 and their parents using a SureSelect Human All Exon kit (Agilent Technologies) for targeted enrichment ...
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bioRxiv - Neuroscience 2021Quote: ... Microgliosis and the presence of protease resistant-prion deposits were visualized by staining brain sections with GFAP (1∶1000, Agilent technologies), IBA1 (1∶2500 ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR was performed with 5 μl of 20x-diluted cDNA and a primer concentration of 1 μM in a 20 μl reaction with Brilliant III Ultra-Fast SYBR GREEN Master Mix (Agilent, www.agilent.com) with a BioRad CFX Connect thermocycler (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Samples were subsequently fixed with 1% formalin and samples acquired and analyzed by flow cytometry (Novocyte) using NovoExpress software (Agilent Technologies).
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bioRxiv - Microbiology 2020Quote: ... The newly constructed pAdtrack-VP2/VP3/VP4 vectors were linearized with Pme I digestion and then cotransformed with pAdEasy-1 vector (AdEasy Adenoviral vector system; Stratagene, USA)[49] into E ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Immunology 2020Quote: ... The primary antibodies used for immunohistochemistry were: antihuman HLA-DR (referred to from here as MHC-II) (Dako M0746; 1:40); anti-human CD3 (Dako A0452 ...
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bioRxiv - Neuroscience 2021Quote: ... The quality of the libraries is determined using the Standard High sensitivity NGS Fragment analysis kit (DNF-474, 1-6000 base pair) on the Agilent Fragment analyzer (Agilent, USA), yielding approximately 260 bp size fragments ...
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bioRxiv - Developmental Biology 2020Quote: ... The sections were washed in TBS and Tween-20 and stained for 1 hour with HRP-labelled secondary antibody (Dako K4003). The staining was developed with diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed on 1:10 diluted cDNA using either SYBR-containing Brilliant III Ultra-Fast QPCR Master Mix (Agilent Technologies) and gene-specific primers ...
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bioRxiv - Microbiology 2022Quote: ... ΔNef ANCH3 (HIV-1 ANCH3 IND116A) was obtained by insertional mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent #200522), for integrase (IN ...
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bioRxiv - Biochemistry 2022Quote: ... Point mutations (I91F and K176E) were introduced using the QuickChange II XL Site-Directed Mutagenesis Kit (SI Table 1; Agilent Technologies). The full-length sequence of the PAI-1 constructs was confirmed by Sanger sequencing (SI Table 1) ...
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bioRxiv - Bioengineering 2022Quote: Cells were dissociated from differentiation wells and seeded at 8 × 104 cells well−1 in CM support medium on Matrigel-coated XFe96 monolayer plates (Agilent Technologies). Culture medium was changed the next day to the well’s respective treatment ...
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bioRxiv - Cell Biology 2022Quote: ... The sulfo-SANPAH solution was placed on gels and activated using 1 Joule of 254 nm UV light (Stratagene UV crosslinker). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice and incubated for 1 h at 37°C in the non-CO2 chamber with 180 μl of the assay medium (Agilent; 103575) supplemented with L-glutamine (2 mM ...
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bioRxiv - Cell Biology 2022Quote: ... treated cells were then equilibrated in seahorse XF base medium supplemented with 1g/L glucose at 37°C in non-CO2 incubator for 1 hour prior to analysis using a Seahorse XF24 Extracellular Flux Analyzer (Seahorse Bioscience).
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bioRxiv - Immunology 2022Quote: ... The biotinylated antibodies were detected with HRP-conjugated streptavidin (for two replicate dilution series) and with a 1:1000 dilution of HRP-conjugated anti-mouse antibody (Dako, P0260) (for another two replicate dilution series) ...
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bioRxiv - Genomics 2022Quote: ... in a final volume of 50 μL and were diluted to 1.5 ng uL-1 to be measured in the High-Sensitivity ScreenTape D1000 reagents (Agilent Technologies, Germany). We included blank controls in all laboratory processes together with our samples ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently rinsed with PBS supplemented with 0.01% Tween 20 and probed with horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (1 in 500 dilution) (DAKO, Agilent Technologies) for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... containing 1 µM sulfadimethoxine as an internal standard before analyzing 15 µL on a 1290 Infinity II UHPLC system (Agilent Technologies) coupled to an ImpactII ultra-high resolution Qq-TOF mass spectrometer (Bruker Daltonics ...
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bioRxiv - Microbiology 2023Quote: ... all tissue section slides were treated with a mouse monoclonal Anti-Human IgG p27KIP1 (Clone SX53G8.5 dilution 1:100; Code M7203; Dako Cytomation, Denmark) that cross-reacts with p27kip1 using a protocol described previously [24] ...
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bioRxiv - Microbiology 2024Quote: ... however with GFP Mouse mAb as a primary antibody diluted 1:1000 in PBST (Cell Signalling, 4B10) and Anti-mouse peroxidase (Dako, P0447) as a secondary antibody diluted 1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-CD61 immunohistochemistry (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:75, Clone Y2/51, Dako Omnis, Code Number: M0753) was also employed to evaluate the presence of platelet-enriched microthrombi ...
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bioRxiv - Neuroscience 2022Quote: A one-in-six series of coronal brain sections from each mouse were stained on slides using a modified immunofluorescence protocol22 for the following antibodies - astrocytes (rabbit anti-GFAP, 1:1000, Agilent Z0334), microglia (rat anti-mouse CD68 ...
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bioRxiv - Neuroscience 2024Quote: Mouse hind paws were fixed in Zamboni’s fixative for 24-48 hours at 4°C and processed as previously described.44 50 μm floating tissue sections were stained with the primary antibody rabbit anti-protein-gene-product 9.5 (PGP 9.5) (Dako, Z5116, 1:500) overnight and the secondary biotinylated goat anti-rabbit IgG antibody (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of the library was hybridized to the Agilent SureSelect XT Human All Exon V4 probes (Agilent Technologies 5190-4634). The targeted exon fragments were captured by Streptavidin beads (Fisher 65602) ...
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bioRxiv - Bioengineering 2024Quote: ... IHC antibodies: rabbit anti-EGFR (clone SP84, Cell Marque #14R-15) at 1:25 and mouse anti-Ki67 (clone MIB1, Dako #M7240) at 1:50 with ME Kit ...
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bioRxiv - Evolutionary Biology 2024Quote: The prepared carbon dots were studied using UV−vis (1 cm cell quartz cuvette) in a Cary 100 UV−vis spectrophotometer (Agilent Technologies) in DI water ...
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bioRxiv - Molecular Biology 2024Quote: ... washed in TBST and incubated for 1-2 h with the peroxidase-coupled secondary antibody (HRP-coupled anti-rabbit IgG (Dako, #P0448), HRP-coupled anti-mouse IgG (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed three consecutive times with Tris buffer (5min) and 1:10000 diluted donkey- or goat-anti-rat-HRP (Agilent Technologies) or 1:25000 diluted goat-anti-mouse-HRP (BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: The total RNA extracted from the floral meristem of species listed in Supplementary Table 1 were checked for quality using RNA ScreenTape (Agilent Technologies) and Qubit 2.0 fluorometer was used for determining the concentration ...
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bioRxiv - Microbiology 2023Quote: ... skimmed milk powder and probed with desired primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1:5000 (Dako Agilent). Protein bands were detected using a G:BOX (Syngene ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and for fragment size on a Fragment Analyzer using the HS NGS Fragment kit (1–6000 bp) (Agilent Technologies Inc., USA). Final enriched libraries were pooled at equimolar quantities ...
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bioRxiv - Microbiology 2022Quote: ... was performed to remove adapter dimers and 1 μl of the cleaned pool was visualised using the Agilent High Sensitivity DNA Kit for Bioanalyzer 2100 (Agilent Technologies) to confirm adapter-dimer removal.
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bioRxiv - Immunology 2023Quote: ... brucei parasites using a polyclonal rabbit antibody raised against T. brucei luminal binding protein 1 (BiP) (J. Bangs, SUNY, USA) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s Haematoxylin ...
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bioRxiv - Immunology 2023Quote: Real-time analysis of oxygen consumption rates (OCR) were measured in THP-1 cells using XF24/96 Extracellular Flux Analyzer (Seahorse Bioscience). Cells were seeded in Seahorse XF96 cell culture microplate (#101085-004 ...
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bioRxiv - Cell Biology 2023Quote: ... frozen femur sections were blocked with 3% BSA and 5% goat serum in PBS, incubated with Cgrp antibody (rabbit polyclonal, abcam #ab47027, 1:300 in DAKO antibody diluent (Agilent, #S0809)) ...
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bioRxiv - Cell Biology 2023Quote: ... gene expression libraries were constructed using 1-100 ng of each amplified cDNA library and quantified using the TapeStation High Sensitivity D1000 kit (Agilent, Germany) to determine the average fragment size and library concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed a further 3 times in TBS-T for 5 minutes and developed with DAB diluted at a 1:50 ratio in DAB-chromagen (Agilent; #K3468) until appearance of brown staining ...