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Citations for Agilent :
3151 - 3200 of 4015 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and the constructed AAV-vector plasmid were transfected into AAV-293 cells (#240073, Agilent) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The cell index was recorded in 15-minute intervals using xCELLigence® RTCA (Agilent) to ensure the cells reached the confluent stage before the assay ...
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bioRxiv - Cell Biology 2024Quote: HUVECs were seeded in the Seahorse XF24 cell culture plate (Agilent, Santa Clara, CA). Wells coated with 0.2% gelatin were used for background subtraction ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 µM Antimycin A + Rotenone (Seahorse XF Cell Mito Stress Test Kit, Agilent) were added sequentially to the Agilent Seahorse XFe96 Extracellular Flux Analyzer via injection ports to determine basal and maximum respiration ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSC and progenitors were seeded on XF96 cell culture microplates (Cat# 102416-100, Agilent). On the day of the experiment ...
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bioRxiv - Immunology 2024Quote: ... cells were treated at different time points with Etomoxir (4 µM; 103672-100; Agilent), Oligomycin (1.5 µM ...
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bioRxiv - Cancer Biology 2024Quote: Cells were washed 3 times with 100 µL of XF Base Media (Seahorse Bioscience) containing 2 mM L-Glutamine ...
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bioRxiv - Immunology 2024Quote: ... Cells counts were determined by flow cytometer Novocyte (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Neuroscience 2020Quote: ... the myoblasts were incubated overnight at 4 °C with primary antibodies against sarcomeric myosin, MF-20 (Developmental Studies Hybridoma Bank, Iowa City, IA, 1:200) and Desmin (Dako, Santa Clara, CA, 1:100). After washing with PBS ...
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products, catalog number GA64461-2). However ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies diluted in blocking solution were incubated overnight at 4°C (anti-GFAP z0334 DAKO 1:400, anti-Iba1 019-19741 Wako 1:400). After three washes in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were examined in a detergent insolubility assay adapted from Drisaldi et al.43 and the proteins in the supernatant and pellet were analyzed by Western blot using the anti-tau rabbit polyclonal antibody DAKO (Agilent Technologies Italy SpA, Milan, Italy) (1:10000 dilution) ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 hour at room temperature followed by treatment with HRP-conjugated anti-mouse secondary antibody (DAKO Envision Plus HRP kit, Dako Denmark A/S, Glostrup, Denmark) for 30 minutes ...
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bioRxiv - Physiology 2019Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:750, DAKO; diluted in 1% goat serum/PBS). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Cell Biology 2021Quote: ... Dissected aortae were fixed in 4% paraformaldehyde for 30 minutes and then washed with 0.1% Triton X-100 in PBS at room temperature, followed by incubation rabbit anti-β-Catenin antibody (Sigma #C2206, St. Louis, MO in blocking solution (Dako-Agilent, K800621, Carpinteria, CA) (dilution 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... then incubated in 5 μg/ml primary antibody in incubation buffer (anti-Mala s 1 mouse monoclonal IgG1 antibody (9G9) from Karolinska Institute (Zagari A et al, 1994, Schmidt et al, 1997) or mouse monoclonal IgG1 antibody (Agilent Dako X093101-2; Santa Clara, USA) as an isotype control for 90 min ...
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bioRxiv - Pathology 2022Quote: ... sections were incubated overnight at 4°C with a primary monoclonal antibody against CYP24A1(sc-365700, Santa Cruz Biotechnology Inc, CA, USA. The sections were then incubated with EnVision (Dako, Agilent, Santa Clara, CA, USA) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... the cells were washed once with the same buffer and incubated with FITC-conjugated polyclonal swine anti-rabbit immunoglobulin antibody from DAKO (dilution 1:40 in BP) for 1 hr at 4°C on ice with shaking ...
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bioRxiv - Microbiology 2022Quote: ... The resulting membrane was blocked for 1 hr in PBS + 5% BSA (Fisher Bioreagents, BP1605-100) before being incubated overnight with primary antibodies [anti-VP1 (Dako, anti-enterovirus clone5-D8/1) or anti-eIF4G (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... Blocking was performed in 0.03% H2O2 containing sodium azide for 5 minutes and primary antibodies (Table S1) were incubated for 60 minutes before detection (Dako EnVision+ System-HRP labeled Polymer) for 30 minutes and development for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with the required primary antibody (ABri: 338 1:1000 from Ghiso lab; ADan: 5282 1:1000 from Ghiso lab; CD68: 1:150 DAKO; CR3/43: 1:100 DAKO) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... IHC was performed on an automated Ventana Discovery Ultra staining system using primary antibodies recognizing Ki-67 (M7240, DAKO, mouse monoclonal, clone MIB1, 1:50) for 30min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunodetection was carried out by blocking PVDF membranes in 5% (w/v) fat-free dried milk powder followed by incubation overnight in rat anti-FLAG antibodies diluted 1:500 (Agilent Technologies, Santa Clara, CA, USA). After a brief rinse in Tris-buffered saline with Tween™ 20 detergent (TBST) ...
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bioRxiv - Cancer Biology 2023Quote: ... All oligo- conjugated antibodies were generated as previously described and were run on a Bioanalyzer Protein 230 electrophoresis chip (Agilent Technologies, cat. no 5067-1517) to verify successful conjugation6.
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bioRxiv - Genomics 2020Quote: ... Blots were washed three times with TBST for 15min at room temperature and subsequently incubated for 2h at room temperature with HRP-conjugated secondary antibodies (goat anti-rabbit, Dako; rabbit anti-goat, R&D Systems) at a dilution of 1:5000 in TBST ...
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bioRxiv - Pathology 2022Quote: ... sections were incubated overnight at 4°C with a primary monoclonal antibody against CYP24A1(sc-365700, Santa Cruz Biotechnology Inc, CA, USA. The sections were then incubated with EnVision (Dako, Agilent, Santa Clara, CA, USA) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal goat anti-rabbit secondary antibody IgG/HRP (P044801-2; Agilent Technologies/Dako, Santa Clara, 527 CA, United States) diluted 1:2000 in milk ...
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bioRxiv - Biophysics 2021Quote: ... cells were resuspended in 200 μL PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... cells that were kept in GM were washed and incubated in XF Base Medium (Agilent) containing 1 mM sodium pyruvate ...
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bioRxiv - Neuroscience 2022Quote: ... the plasmid was amplified using chemically competent cells deficient for recombinase activity (Agilent Technologies #200152), purified (Macherey-Nagel #740416.10) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and analyzed by flow cytometry (NovoCyte Flow Cytometer Systems, Agilent, CA, USA). The tetramer positive staining wells were pooled and CD8/Tetramer double positive population were sorted using flow cytometric sorting (ARIA II sorter ...
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bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...
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bioRxiv - Developmental Biology 2019Quote: ... cell culture medium was replaced with the appropriate pre-warmed Seahorse XF Base Medium (Agilent Biotechnology ...
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bioRxiv - Immunology 2019Quote: Cell culture supernatants were harvested and concentrated with StrataClean resin beads (Agilent Technologies, CA, USA), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: The pHMT-KTN1 plasmid was transformed into BL21-CodonPlus (DE3)-RIPL competent cells (Agilent Technologies). Protein was affinity-purified using Ni-NTA agarose (Qiagen #301210) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and analyzed by flow cytometry (NovoCyte Flow Cytometer Systems, Agilent, CA, USA). The tetramer positive staining wells were pooled and CD8/Tetramer double positive population were sorted using flow cytometric sorting (ARIA II sorter ...
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bioRxiv - Biochemistry 2021Quote: ... Expression vectors containing gene of interest were transformed into BL21-Gold (DE3) competent cells (Agilent) and the cells were cultured to a density of ~1 OD600/ml at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Barcoded single cell capture plates were prepared with a Bravo automated liquid handling platform (Agilent) as described previously28 ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were tested for mycoplasma using the PCR based mycoplasma detection kit (Agilent).
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bioRxiv - Cell Biology 2021Quote: ... OCR and ECAR were measured using Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). 1 × 104 cells/well were plated onto 7 μg/mL Poly-D-Lysine coated (Ruhanen et al ...
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bioRxiv - Neuroscience 2020Quote: ... Washing procedure was repeated and cells were coversliped using an aqueous fluorescent mounting medium (Dako). The specimens were analysed under a confocal microscope Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Neuroscience 2020Quote: Mitochondrial function was assessed using a Seahorse XF24 cell MitoStress Test (Agilent Technologies, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... mitochondrial stress test was performed using a Seahorse XF Cell Mito Stress Test kit (Agilent) according to the manufacture manual ...
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bioRxiv - Microbiology 2020Quote: ... Pt cones and an Octopole Reaction System (ORS) collision cell (Agilent Technologies, Santa Clara, CA). The data was collected using a 3-point peak pattern ...
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bioRxiv - Cell Biology 2020Quote: ... Barcoded single cell capture plates were prepared with a Bravo automated liquid handling platform (Agilent) as described previously16 ...
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bioRxiv - Biophysics 2020Quote: The hSirt3102-399 was expressed in E.coli Arctic Express (DE3) cells (Agilent Technologies, Wilmington, DE) as per manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV plasmids were co-transfected with a pDP6 helper plasmid into HEK293-AAV cells (Agilent). Cells were lysed 72 h after transfection and viral particles were purified using Iodixanol gradient followed by separation ion-exchange chromatography (GE Healthcare) ...