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Citations for Agilent :
3001 - 3050 of 3556 citations for 6 Methoxy 3 4 Dihydro 2H Quinoxaline 1 Carboxylic Acid tert Butyl Ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... incubated with DAPI (1:10’000) to label nuclei for 10 min and mounted using Fluorescence mounting medium (Dako, S3023). Fluorescent images were recorded with a Leica SP5 Confocal Microscope.
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bioRxiv - Neuroscience 2020Quote: ... and followed by incubation with primary antibodies or negative isotype controls diluted in blocking buffer for 1 hour (Covance PRB-278P rabbit polyclonal IgG anti-PAX6, 10μg/mL; Dako Z0334 rabbit polyclonal IgG anti-GFAP ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei staining with Hoechst (1:10.000 in PBS) applied between initial washes or with ProLong Gold DAPI mounting medium (Dako). Micrographs were recorded using a Zeiss LSM710 point laser (Argon Lasos RMC781272 ...
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bioRxiv - Cancer Biology 2019Quote: ... This was followed by sequential 1-hour incubations with the secondary antibodies (En Vision+ System-HRP-Labeled Polymer; Dako) and visualization with the Liquid DAB+ Substrate Chromogen System (Dako) ...
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bioRxiv - Neuroscience 2020Quote: ... was generated by site directed mutagenesis using primers oM18_17 and oM18_18 (Table 1) and a Quikchange II Site-directed Mutagenesis kit (Agilent, 200523). The PCR product was digested with DpnI enzyme to remove template vector and transformed into Stbl2 chemically competent cells (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... and cDNA library size was checked using Fragment Analyzer HS NGS Fragment Kit (1-6000bp) (Agilent formerly Advanced Analytical). Only high-quality cDNA libraries without notable degradation were used for sequencing library construction ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA concentration and quality were assessed using the Advanced Analytical Fragment Analyzer (Agilent Technologies, CA, USA, table 1) prior to library construction ...
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bioRxiv - Biochemistry 2020Quote: ... equipped with a DB-1 MS capillary column (15 m x 0.25 mm x 0.25 μm, Agilent 122-0112). Chromatography was conducted with helium gas ...
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bioRxiv - Plant Biology 2020Quote: ... The powder (1 g) was immediately transferred to a 20-mL head-space vial (Agilent, Palo Alto, CA, USA) containing 2 mL NaCl-saturated solution to inhibit any enzyme reaction ...
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bioRxiv - Systems Biology 2021Quote: ... and a mass accuracy of <1 ppm was often achieved using the standard tuning mixture (G1969-85000, Agilent Technologies). The m/z scan range was 100−2000 ...
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bioRxiv - Immunology 2021Quote: Synthesised antigen constructs were transformed into competent XL-1 Blue Escherichia coli cells (Agilent Technologies, Santa Clara, CA, USA) by electroporation ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies and dilutions used were as follows: anti-Mouse Immunoglobulins/FITC (1:400, F0479, Dako, Carpinteria, CA, USA) and anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Single cells were separated using a 100 μm strainer and labelled with CD31 antibody (1:200, M082329-2, DAKO) at 4°C for 45 min followed by 30 min staining with a goat anti-mouse secondary antibody conjugated to AlexaFluor 488 or 555 (1:400 ...
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bioRxiv - Biophysics 2020Quote: ... in 20 min at a flow rate of 0.4 mL min-1 using a Zorbax Narrow Bore (2.1×50 mm, 5 μm) C18 column (Agilent Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... Primary Axl antibody (RnD Systems, AF154) was diluted 1:400 in Antibody Diluent with Background Reducing Components (Dako, S3022). 200 µL of antibody solution was put on each slide to cover all TMA specimens and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies used were Polyclonal Goat Anti-Rabbit or Polyclonal Rabbit Anti-Mouse (1:1000) HRP-conjugated (Dako Agilent), or IRDye® 680RD (1:20,000)- or 800CW (1:10,000)-conjugated for quantitative fluorescence measurements on an Odyssey® Fc Dual-Mode Imaging System (LICOR Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies used were Polyclonal Goat Anti-Rabbit or Polyclonal Rabbit Anti-Mouse (1:1000) HRP-conjugated (Dako Agilent), or IRDye® 680RD (1:20,000)- or 800CW (1:10,000)-conjugated for quantitative fluorescence measurements on an Odyssey® Fc Dual-Mode Imaging System (LICOR Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products ...
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bioRxiv - Immunology 2022Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Basal OCR and ECAR were measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies were followed by respective HRP-conjugated anti-mouse or anti-rabbit secondary antibody (1: 2000; Dako). Detection of peroxidase activity was performed by using the Amersham ECL western blot detection kit (GE Healthcare).
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bioRxiv - Genetics 2022Quote: ... followed by incubation with goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP) (1:2000, Agilent Cat# P0448, RRID:AB_2617138). Ubiquitin was detected using mouse monoclonal anti-ubiquitin antibody (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... retinal cross-sections were treated with antigen retrieval solution at 99 °C for 1 h (PH 6.1; Dako #S1699) and washed in 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used were listed as follows: rabbit anti-GFAP (a marker for astrocytes, 1:1000, Cat# Z0334, DAKO), rabbit anti-NeuN (a marker for neurons 1:1000 ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation variants found in P.1 and 10-mutation variant (BZΔ10) were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana VSV (rVSV ...
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bioRxiv - Molecular Biology 2020Quote: ... XL10-Gold ultracompetent cells (Tetr Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F’proAB lacIqZΔM15 Tn10 (Tetr) Amy Camr] (Agilent Technologies) were used.
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bioRxiv - Cancer Biology 2021Quote: ... 2% BSA (PAA Laboratories GmbH, Germany) and 1:50 dilution of normal goat serum (Dako Denmark A/S, Denmark). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei were stained with DAPI (1:500 in 0.1M PBS) and mounted on glass slides using fluorescent mounting media (DAKO) and examined in a fluorescence Observer Z1 microscope with ApoTome 2 under a Plan-Apochromat 40x/1.3 Oil DIC objective (Carl Zeiss) ...
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bioRxiv - Biochemistry 2022Quote: ... Sample from each PNT3 variant at 5 mg mL-1 in buffer B containing 6M GDN was injected onto an AdvanceBio SEC 2.7 µm (Agilent) SEC column ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with 1 × blocking buffer (5% goat serum [#X0907, Dako], 2.5% BSA, 0.1% Triton X-100 in PBS) (38) ...
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bioRxiv - Biochemistry 2019Quote: ... 1 µl sample was injected in splitless mode with an Agilent 7693 autosampler injector into deactivated splitless liner (Agilent 5190-3167 ...
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Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetesbioRxiv - Cell Biology 2019Quote: ... Pancreas tissue sections (8 um) were stained using the following primary antibodies: guinea pig anti-insulin (DAKO; 1:500), goat anti-pancreatic polypeptide (abcam ...
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bioRxiv - Biophysics 2020Quote: ... After rinsing for 5 minutes in PBS they were incubated with secondary antibody (Rabbit Anti-Mouse 1/200, Dako) for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The sample (1 μL) was injected onto a VF5-MS column (30 m × 0.25 mm × 0.25 μm; Agilent Technologies) with an inlet temperature of 280 °C and a split ratio of 20:1 ...
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bioRxiv - Immunology 2020Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Pathology 2021Quote: ... The slides were treated with 1× Dako Peroxidase-Blocking Solution® (cat S2023; Agilent Technologies Inc., Santa Clara, CA) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were probed with appropriate primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1/5000 (Dako Agilent). Proteins were detected with ECL Western Blotting Substrate (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were probed with appropriate primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1/5000 (Dako Agilent). Proteins were detected with ECL Western Blotting Substrate (Promega ...
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bioRxiv - Cancer Biology 2019Quote: ... the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies). The enzymatic reaction was conducted with 1-Step Ultra TMB-ELISA (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... samples were passed through an immobilized pepsin column (2mm × 2cm) at 50μl min-1 and the digested peptides were captured on a 2mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was transferred to nitrocellulose membrane for 1 hour via vacuum transfer as described in Stratagene’s Membranes Instruction Manual (Stratagene) and crosslinked using a Stratalinker UC Crosslinker (Stratagene) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μl of each RNA extract was used for testing RNA quality and quantity using a Bioanalyzer Systems (Agilent) at the LUMC hospital in Leiden (the Netherlands ...
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bioRxiv - Neuroscience 2021Quote: ... Heat-based antigen retrieval was performed using 1× antigen retrieval solution at pH 9 (Agilent Technologies; Santa Clara, CA) for 1 hour (30 min at 95C ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked in 0.2% triton X-100 solution containing 1% BSA and 5% normal goat serum (DAKO, X0907) for 1 hour.
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bioRxiv - Immunology 2020Quote: ... A total of 1 × 106 PBMC was seeded in 24-well XF24 cell culture microplates (Agilent Technologies, Waldbronn, Germany), while four wells were kept free from cells as background correction ...
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bioRxiv - Immunology 2021Quote: ... and a 1:10 dilution of the resulting cDNA was run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess cDNA quality and yield ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated for 1hr at room temperature with polyclonal HRP-conjugated goat anti-rabbit (1:5000; Dako, Denmark) or anti-mouse (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were then incubated with anti-PD-L2 (CST #82723) diluted 1:25 in EnVision FLEX antibody diluent (Dako) over night at 4º C ...
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bioRxiv - Biochemistry 2022Quote: ... The column exit was connected to the ESI source of the mass spectrometer using PEEK tubing (0.005” inner diameter × 1/16” outer diameter, Agilent). Mass spectra were acquired in the positive ion mode over the range m/z = 350 to 1800 using the Orbitrap mass analyzer ...
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bioRxiv - Cell Biology 2022Quote: ... the cell nuclei were stained with a 1:2000 dilution of SPY-DNA dye (Spirochrome) in antibody diluent (Dako). SPY-DNA 505 ...
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bioRxiv - Immunology 2022Quote: ... The tetrameric detection reagents were generated immediately prior to use by combining biotinylated FcɣR proteins (Ackerman Laboratory) with a 1/4th molar ratio of Streptavidin-Allophycocyanin (ProZyme) and mixing by inversion for 10 min ...