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Citations for Agilent :
2951 - 3000 of 3581 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade AE CM240 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The primary incubation was performed overnight at 4°C (phospho-Ser129, BioLegend Cat # 825701, 1:2000; GFAP, DAKO Cat # GA524 ...
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bioRxiv - Neuroscience 2024Quote: ... 1:300) was used with the Dako High pH Target Retrieval Solution (Tris/EDTA, pH 9; Agilent, USA) (20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibody in TBS-T (1:3000, Dako, Glostrup, Denmark) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 105 CUTLL1 were plated per well on poly-D-lysine coated Seahorse XFe96 microplates (Agilent #101085-004) in XF RPMI medium (Agilent #103576-100 ...
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bioRxiv - Cell Biology 2024Quote: ... MONX10283) followed by goat-anti-rabbit or goat-anti-mouse conjugated to horseradish peroxidase (1:2000, Dako, P0448) and then visualized (VS200 slide scanner ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA fragment sizes and integrity were checked with a 1% agarose gel and a Femto Pulse system (Agilent). Long DNA fragments were sequenced from a single male individual using Nanopore PromethION in one flongle cell and run for 72 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were counterstained with DAPI for 1 min at RT and mounted with Fluorescence Mounting Medium (S3023; Dako). Fluorescence was observed with a SP8-DLS confocal microscope (Leica Microsystems) ...
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bioRxiv - Plant Biology 2023Quote: ... A 1-µL aliquot was injected in splitless mode into a VF-5ms capillary column (Varian CP9013, Agilent). The GC was operated at a constant helium flow of 1 mL per minute and the injector was set to 280°C ...
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bioRxiv - Microbiology 2023Quote: ... BQ.1.1 and XBB.1 was constructed by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) using the BA.2 or BA.4/5 SΔ19 gene as a template ...
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bioRxiv - Neuroscience 2023Quote: ... 5–20 ms at 1 Hz) controlled by a Picospritzer III (General Valve) and a pulse generator (Agilent). The speed of injection was ∼0.1 μl/10 min ...
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bioRxiv - Biochemistry 2023Quote: ... coli (genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacIqZΔM15 Tn10(Tetr)]) from Agilent Technologies were used for cloning ...
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bioRxiv - Cell Biology 2023Quote: ... Library quality was assessed on a fragment analyser instrument using SS NGS fragment kit (1-6,000 bp; Agilent). Sequencing was performed on NovaSeq 6000 platform using a 200 cycles SP Reagent Kit v1.5 (both Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... in a citrate buffer for 20 min at 95°C for TP53 (1:800, Dako, M7001 DO-7), BCL-10 (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-rabbit or –mouse IgG HRP-linked Antibody (#P0399 and #P0260, DAKO GmbH, Jena, Germany; WB: 1:400) and goat anti-Rabbit IgG Alexa Fluor 555 (#A-21428 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of RNA was reverse transcribed using random hexamers and the AffinityScript Multi-Temp reverse transcriptase (Agilent) following the manufacturer’s instructions ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... as the first dimension (1D) column and VF-17ms (1 m × 0.1 mm I.D. × 0.1 μm df; Agilent Technologies) as the second dimension (2D ...
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bioRxiv - Molecular Biology 2023Quote: ... the sections were washed and horseradish peroxidase conjugated secondary antibody Rabbit Anti-Mouse (1:100, P0260, Dako, Denmark) was added to FBLN2 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibody in TBS-T (1:3000, Dako, Glostrup, Denmark) for 1 hour at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Then slides were incubated with E2F1 (VD301790, Thermo Fischer, 1:100 diluted in Dako REAL Antibody Diluent S2022) or E2F8 antibody (ab185727 ...
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bioRxiv - Evolutionary Biology 2023Quote: Mutants were constructed by: 1) site-directed mutagenesis as described in the QuickChange Site-Directed Mutagenesis manual (Agilent), or 2 ...
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bioRxiv - Plant Biology 2023Quote: ... We assessed the genomic integrity of a 1:100 dilution using a TapeStation (Agilent Technologies, Santa Clara, USA).
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bioRxiv - Microbiology 2024Quote: ... coli XL1-Blue (recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac) (Stratagene/ Agilent, Santa Clara, CA, USA), E ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were then incubated overnight at 4 °C with appropriate dilution of anti-Ki67 (1:100, Dako, M7240) in TBS containing Horse Serum (2%) ...
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bioRxiv - Immunology 2024Quote: ... 4×105 treated NK cells were resuspended in Seahorse XF DMEM Medium containing glutamine (1 mM; Agilent, USA) and seeded into an 8-well XF cell culture microplate (Agilent ...
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bioRxiv - Microbiology 2024Quote: ... sections were blocked for 1 h at room temperature in serum-free blocking buffer (Agilent Technologies, X090930-2), and then incubated with polyclonal rabbit antibody to ASBT (kindly provided by Paul Dawson ...
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bioRxiv - Microbiology 2024Quote: ... coli XL1-Blue (recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac) (Stratagene/ Agilent, Santa Clara, CA, USA), E ...
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bioRxiv - Biochemistry 2024Quote: ... delivered at a flow rate of 200 μL.min−1 (1260 Infinity II Quaternary pump, Agilent Technologies, Waldbronn, Germany). Desalted peptides were then separated on a reversed-phase analytical column (LUNA Omega Polar C18 Column ...
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bioRxiv - Microbiology 2020Quote: ... Sections were then incubated with the primary antibody (diluted at 1 µg/ml in Antibody Diluent [Dako, Carpinteria, CA]) for 30 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) TFA as mobile phase on a 4.6 mm x 250 mm Zorbax 300SB-C8 column (Agilent) at 80 °C column temperature and 1 ml min-1 flow rate (Supplementary Fig ...
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bioRxiv - Biophysics 2020Quote: ... Optical absorption spectra of the samples in a 300-900 nm range were recorded within about 10 s at given time intervals (about 0.3-1.0 min) in a 3 mL quartz cuvette (Helma QS1000, 1 cm pathlength) using a Cary 60 spectrometer (Agilent). Alternatively ...
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bioRxiv - Cancer Biology 2021Quote: The appropriate pGEX-6P-1 constructs were transformed into the chemically competent BL21-Codon Plus (DE3)-RIPL (Agilent Technologies) E ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed three times with PBS containing 0.05% Tween-20 and incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibodies (DAKO) at a dilution of 1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of this solution was subjected to TapeStation RNA ScreenTape Analysis (Agilent #5067-5576, #5067-5577, #5067-5578) to detect ssDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was purified using 1.8:1 AMPure XP Beads: DNA ratio and libraries were quantified and sized on a D1000 Tapestation tape (Agilent). Libraries ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then incubated for 1 hour at 37°C in atmospheric CO2 in pH 7.4 unbuffered RPMI1640 with 1.67 mM glucose (Seahorse bioscience). Islets were first stimulated with high glucose (16.7 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... DAPI (1:500, Dojindo) was used for nuclear staining and the sections were mounted with fluorescence mounting medium (Dako). Fluorescent images were captured using an all-in-one fluorescence microscope (BZ-X700 ...
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated in secondary antibody solutions (TBS containing 0.1 % Tween-20 and 5 % BSA, HRP-conjugated secondary antibodies (1:5000, Dako)) at room temperature ...
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bioRxiv - Immunology 2022Quote: ... CF (R&D/BT10500-050) was incubated at a 4:1 molar ratio with either streptavidin-PE (Prozyme PJRS25) or streptavidin-APC (Prozyme PJ27S ...
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bioRxiv - Genomics 2020Quote: Twenty µg of high molecular weight (HMW) DNA (50 ng µl-1) was evaluated on a Fempto Pulse (Agilent) with a three hour run time and converted to SMRTbell® libraries using SMRTbell® Express Template Prep kit according to the manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Site mutagenesis was performed on pCMV4 CYP2D6*1 plasmid23 with QuikChange II Site-Directed Mutagenesis Kit (Agilent, CA, US). Plasmid cDNA encoding variants with the following amino acid exchanges were created ...
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bioRxiv - Neuroscience 2019Quote: ... The blots were washed again and an alkaline-phosphate-conjugated goat anti mouse antibody was added (DAKO, 1:2000). Another washing step with TBST and B3 buffer (100 mM Tris ...
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bioRxiv - Developmental Biology 2019Quote: ... DAPI (1:500, Dojindo) was used for nuclear staining and the sections were mounted with fluorescent mounting medium (Dako). At least three embryos of each genotype were used for each analysis.
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bioRxiv - Plant Biology 2019Quote: ... Total RNA quality was examined on 1% agarose gels and RNA Nanochips on an Agilent 2100 Bioanalyzer (Agilent Technologies). Samples with RNA integrity number ≥ 7.0 were used for library preparation ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting 40 samples were diluted 4:1 with Buffer A for Multiple Affinity Removal LC Columns (Agilent Technologies), filtered through a 0.22 μm hydrophilic PVDF membrane filter plate (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... with 5% BSA and 0.4% triton X-100) followed by incubation overnight at 4°C with 1:500 rabbit anti-C3 antibody (Dako) and 1:10,000 guinea pig anti-vGluT2 (Synaptic Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with DAPI (1:10’000) to label nuclei for 10 min and mounted using Fluorescence mounting medium (Dako, S3023). Fluorescent images were recorded with a Leica SP5 Confocal Microscope.
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bioRxiv - Neuroscience 2020Quote: ... and followed by incubation with primary antibodies or negative isotype controls diluted in blocking buffer for 1 hour (Covance PRB-278P rabbit polyclonal IgG anti-PAX6, 10μg/mL; Dako Z0334 rabbit polyclonal IgG anti-GFAP ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei staining with Hoechst (1:10.000 in PBS) applied between initial washes or with ProLong Gold DAPI mounting medium (Dako). Micrographs were recorded using a Zeiss LSM710 point laser (Argon Lasos RMC781272 ...